ISO 8784-2

Pulp, paper and board — Microbiological examination — Part 2: Enumeration of bacteria, yeast and mould on surface

Pâtes, papiers et cartons — Analyse microbiologique — Partie 2: Dénombrement des bactéries, des levures et des moisissures en surface

General Information

• Status: Not Published
• ICS: 07.100.99 - Other standards related to microbiology
• ICS: 85.040 - Pulps
• ICS: 85.060 - Paper and board
• Technical Committee: ISO/TC 6/SC 2 - Test methods and quality specifications for paper and board
• Drafting Committee: ISO/TC 6/SC 2/WG 26 - Microbiological test methods
• Current Stage: 6000 - International Standard under publication
• Completion Date: 14-Apr-2023

Standards Content (Sample)

FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 8784-2
ISO/TC 6/SC 2
Pulp, paper and board —
Secretariat: SIS
Microbiological examination —
Voting begins on:
2023-02-16
Part 2:
Voting terminates on:
Enumeration of bacteria, yeast and
2023-04-13
mould on surface
Pâtes, papiers et cartons — Analyse microbienne —
Partie 2: Dénombrement des bactéries, des levures et des moisissures
en surface
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/FDIS 8784-2:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023
---------------------- Page: 1 ----------------------
ISO/FDIS 8784-2:2023(E)
FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 8784-2
ISO/TC 6/SC 2
Pulp, paper and board —
Secretariat: SIS
Microbiological examination —
Voting begins on:
Part 2:
Voting terminates on:
Enumeration of bacteria, yeast and
mould on surface
Pâtes, papiers et cartons — Analyse microbienne —
Partie 2: Dénombrement des bactéries, des levures et des moisissures
en surface
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023

All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may

be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on

the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below

or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: [phone]
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: [e-mail]
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/FDIS 8784-2:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
© ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023
---------------------- Page: 2 ----------------------
ISO/FDIS 8784-2:2023(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

Introduction .................................................................................................................................................................................................................................v

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ..................................................................................................................................................................................... 1

3 Terms and definitions .................................................................................................................................................................................... 1

4 Principle ........................................................................................................................................................................................................................ 2

5 Culture media - Contact plates for bacteria, yeast and mould ............................................................................ 2

6 Apparatus .................................................................................................................................................................................................................... 2

7 Sampling ....................................................................................................................................................................................................................... 3

8 Preparation of the test material.......................................................................................................................................................... 3

9 Procedure ....................................................................................................................................................................................................................3

9.1 Conditions ................................................................................................................................................................................................... 3

9.2 Collection of bacteria, yeast and mould ........................................................................................................................... 3

9.3 Incubation ................................................................................................................................................................................................... 5

10 Enumeration of the colonies ....................................................................................................................................................................5

11 Expression of results and report ........................................................................................................................................................ 6

11.1 Expression of results ........................................................................................................................................... .............................. 6

11.2 Interpretation .......................................................................................................................................................................................... 6

12 Report .............................................................................................................................................................................................................................. 6

iii
© ISO 2023 – All rights reserved
---------------------- Page: 3 ----------------------
ISO/FDIS 8784-2:2023(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www.iso.org/patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO's adherence to

the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see

www.iso.org/iso/foreword.html.

This document was prepared by Technical Committee ISO/TC 6, Paper, board and pulps, Subcommittee

SC 2, Test methods and quality specifications for paper and board.
A list of all parts in the ISO 8784 series can be found on the ISO website.

Any feedback or questions on this document should be directed to the user’s national standards body. A

complete listing of these bodies can be found at www.iso.org/members.html.
© ISO 2023 – All rights reserved
---------------------- Page: 4 ----------------------
ISO/FDIS 8784-2:2023(E)
Introduction

Because of the exacting techniques required in aseptic procedures, reproducible, good quality results

can only be ensured by skilled microbiological technicians.
© ISO 2023 – All rights reserved
---------------------- Page: 5 ----------------------
FINAL DRAFT INTERNATIONAL STANDARD ISO/FDIS 8784-2:2023(E)
Pulp, paper and board — Microbiological examination —
Part 2:
Enumeration of bacteria, yeast and mould on surface
1 Scope

This document specifies a method for determining the bacteria, yeast and mould population on the

surface of paper and paperboard. The enumeration relates to specific media.

This document is applicable to all kinds of paper and paperboard, to dry market pulp in sheet form and

to packaging material.
2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 186, Paper and board — Sampling to determine average quality
ISO 7213, Pulps — Sampling for testing

ISO 7218, Microbiology of food and animal feeding stuffs — General rules for microbiological examinations

3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.

ISO and IEC maintain terminology databases for use in standardization at the following addresses:

— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
bacteria

microscopic, single-cell organisms that possess a prokaryotic type of cell structure, which reproduce

by fission a
...

© ISO 2022 – All rights reserved
ISO/FDIS 8784-2:2022 (E)
2022-12-19
ISO/TC 6/SC 2
Secretariat: SIS
Date: 2023-02-02
Pulp, paper and board — Microbiological examination —
Part 2:
Enumeration of bacteria, yeast and mould on surface
Pâtes, papiers et cartons — Analyse microbienne —
Partie 2: Dénombrement des bactéries, des levures et des moisissures en surface
FDIS stage
© ISO 2023 – All rights reserved
---------------------- Page: 1 ----------------------
ISO/FDIS 8784-2:20222023(E)
© ISO 20212023

All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this

publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical,

including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can

be requested from either ISO at the address below or ISO’s member body in the country of the requester.

ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
EmailE-mail: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2022 2023 – All rights reserved
---------------------- Page: 2 ----------------------
ISO/FDIS 8784-2:2022 2023(E)
Contents

Foreword ............................................................................................................................................................................................ iv

Introduction ........................................................................................................................................................................................ v

1 Scope ....................................................................................................................................................................................... 1

2 Normative references ....................................................................................................................................................... 1

3 Terms and definitions ...................................................................................................................................................... 1

4 Principle ................................................................................................................................................................................. 1

5 Culture media - Contact plates for bacteria, yeast and mould ........................................................................ 2

6 Apparatus .............................................................................................................................................................................. 2

7 Sampling ................................................................................................................................................................................ 2

8 Preparation of the test material ................................................................................................................................... 3

9 Procedure .............................................................................................................................................................................. 3

9.1 Conditions ............................................................................................................................................................................. 3

9.2 Collection of bacteria, yeast and mould .................................................................................................................... 3

9.3 Incubation ............................................................................................................................................................................. 4

10 Enumeration of the colonies ......................................................................................................................................... 4

11 Expression of results and report ................................................................................................................................. 5

11.1 Expression of results ........................................................................................................................................................ 5

11.2 Interpretation ...................................................................................................................................................................... 5

12 Report ..................................................................................................................................................................................... 5

© ISO 2022 2023 – All rights reserved iii
---------------------- Page: 3 ----------------------
ISO/FDIS 8784-2:20222023(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO

collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any

patent rights identified during the development of the document will be in the Introduction and/or on

the ISO list of patent declarations received (see www.iso.org/patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO's adherence to the World

Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see

www.iso.org/iso/foreword.html.

This document was prepared by Technical Committee ISO/TC 6, Paper, board and pulps, Subcommittee

SC 2, Test methods and quality specifications for paper and board.
A list of all parts in the ISO 8784 series can be found on the ISO website.

Any feedback or questions on this document should be directed to the user’s national standards body. A

complete listing of these bodies can be found at www.iso.org/members.html.
Field Code Changed
iv © ISO 2022 2023 – All rights reserved
---------------------- Page: 4 ----------------------
ISO/FDIS 8784-2:2022 2023(E)
Introduction

Because of the exacting techniques required in aseptic procedures, reproducible, good quality results can

only be ensured by skilled microbiological technicians.
© ISO 2022 2023 – All rights reserved v
---------------------- Page: 5 ----------------------
ISO/FDIS 8784-2:2023(E)
Pulp, paper and board — Microbiological examination — —
Part 2:
Enumeration of bacteria, yeast and mould on surface
1 Scope

This document specifies a method for determining the bacteria, yeast and mould population on the

surface of paper and paperboard. The enumeration relates to specific media.

This document is applicable to all kinds of paper and paperboard, to dry market pulp in sheet form and

to packaging material.
2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 186, Paper and board — Sampling to determine average quality
ISO 7213, Pulps — Sampling for testing

ISO 7218, Microbiology of food and animal feeding stuffs — General rules for microbiological examinations

3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.

ISO and IEC maintain terminology databases for use in standardization at the following addresses:

— — ISO Online browsing platform: available at https://www.iso.org/obp
— — IEC Electropedia: available at https://www.electropedia.org/
3.1
bacteria

microscopic, single-cell organisms that possess a prokaryotic type of cell structure, which reproduce by

fission and are able to grow under the specific test conditions
3.2
yeast

single-cell fungus, which multiplies mainly vegetatively by budding, able to grow under specific test

conditions
3.3
mould

mycelium forming microfungus, including spores and conidia, able to grow under specific test conditions

3.4
test piece

portion of the paper or paperboard product which is used in the test to evaluate the number of bacteria,

yeast and mould on the surface of the product
4 Principle

This method describes a procedure for determining the number of colony-forming units (CFU) of

bacteria, yeast and mould transferred from the surface of a sample by making a surface print of the

sample on a nutrient agar medium (contact-plate method). Test pieces of paper, paperboard or dry

© ISO 2022 2023 – All rights reserved 1
---------------------- Page: 6 ----------------------
ISO/FDIS 8784-2:2023(E)

market pulp in sheet form are pressed on to the surface of specified agar culture media contact plates for

10 seconds at room temperature. The contact time between the sample and the nutrient agar medium at

room temperature is 5 h for bacteria and 20 h for yeast and mould. The test pieces are then removed and

contact plates are incubated at 37 °C ± 2 °C for 2 d for bacteria and at 25 °C ± 2 °C for 3 d to 5 d for yeast

and mould. At the end of the incu
...