SIST EN ISO 10504:2015
(Main)Starch derivatives - Determination of the composition of glucose syrups, fructose syrups and hydrogenated glucose syrups - Method using high-performance liquid chromatography (ISO 10504:2013)
Starch derivatives - Determination of the composition of glucose syrups, fructose syrups and hydrogenated glucose syrups - Method using high-performance liquid chromatography (ISO 10504:2013)
This International Standard describes a high-performance liquid chromatographic (HPLC) method
for measuring the composition of dextrose solutions, glucose syrups, fructose-containing syrups,
hydrogenated glucose syrups, sorbitol, mannitol and maltitol. The constituents are mainly glucose,
maltose, maltotriose, fructose, sorbitol, mannitol, maltitol and malto-oligosaccharides.
The use of a column packed with cation-exchange resin is essential.
Stärkederivate - Bestimmung der Zusammensetzung von Glucosesirup, Fructosesirup und hydriertem Glucosesirup - Hochleistungs-flüssigchromatographisches Verfahren (ISO 10504:2013)
Produits dérivés de l'amidon - Détermination de la composition des sirops de glucose, des sirops de fructose et des sirops de glucose hydrogénés - Méthode par chromatographie en phase liquide à haute performance (ISO 10504:2013)
ISO 10504:2013 spécifie une méthode d'analyse par chromatographie en phase liquide à haute performance (CLHP) pour déterminer la composition de solutions de dextrose, de sirops de glucose, de sirops contenant du fructose, de sirops de glucose hydrogénés, du sorbitol, du mannitol et du maltitol. Les constituants sont, principalement, le glucose, le maltose, le maltotriose, le fructose, le sorbitol, le mannitol, le maltitol et les maltooligosaccharides.
L'utilisation d'une colonne garnie d'une résine échangeuse de cations est indispensable.
Škrobni derivati - Določevanje sestave glukoznih, fruktoznih in hidrogeniranih glukoznih sirupov - Metoda tekočinske kromatografije visoke ločljivosti (ISO 10504:2013)
Ta mednarodni standard opisuje metodo tekočinske kromatografije visoke ločljivosti (HPLC) za merjenje sestave raztopin dekstroze, glukoznih sirupov, fruktoznih sirupov, hidrogeniranih glukoznih sirupov, sorbitola, manitola in maltitola. Sestavine so večinoma glukoza, maltoza, maltotrioza, fruktoza, sorbitol, manitol, maltitol in malto-oligosaharidi. Nujna je uporaba kolone s kationskim izmenjevalnim gelom.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 10504:2015
01-oktober-2015
1DGRPHãþD
SIST EN ISO 10504:2001
âNUREQLGHULYDWL'RORþHYDQMHVHVWDYHJOXNR]QLKIUXNWR]QLKLQKLGURJHQLUDQLK
JOXNR]QLKVLUXSRY0HWRGDWHNRþLQVNHNURPDWRJUDILMHYLVRNHORþOMLYRVWL ,62
Starch derivatives - Determination of the composition of glucose syrups, fructose syrups
and hydrogenated glucose syrups - Method using high-performance liquid
chromatography (ISO 10504:2013)
Stärkederivate - Bestimmung der Zusammensetzung von Glucosesirup, Fructosesirup
und hydriertem Glucosesirup - Hochleistungs-flüssigchromatographisches Verfahren
(ISO 10504:2013)
Produits dérivés de l'amidon - Détermination de la composition des sirops de glucose,
des sirops de fructose et des sirops de glucose hydrogénés - Méthode par
chromatographie en phase liquide à haute performance (ISO 10504:2013)
Ta slovenski standard je istoveten z: EN ISO 10504:2015
ICS:
67.180.20 Škrob in izdelki iz njega Starch and derived products
SIST EN ISO 10504:2015 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN ISO 10504:2015
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SIST EN ISO 10504:2015
EUROPEAN STANDARD
EN ISO 10504
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2015
ICS 67.180.20 Supersedes EN ISO 10504:2000
English Version
Starch derivatives - Determination of the composition of glucose
syrups, fructose syrups and hydrogenated glucose syrups -
Method using high-performance liquid chromatography (ISO
10504:2013)
Produits dérivés de l'amidon - Détermination de la Stärkederivate - Bestimmung der Zusammensetzung von
composition des sirops de glucose, des sirops de fructose Glucosesirup, Fructosesirup und hydriertem Glucosesirup -
et des sirops de glucose hydrogénés - Méthode par Hochleistungs-flüssigchromatographisches Verfahren (ISO
chromatographie en phase liquide à haute performance 10504:2013)
(ISO 10504:2013)
This European Standard was approved by CEN on 19 June 2015.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10504:2015 E
worldwide for CEN national Members.
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SIST EN ISO 10504:2015
EN ISO 10504:2015 (E)
Contents Page
European foreword .3
2
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SIST EN ISO 10504:2015
EN ISO 10504:2015 (E)
European foreword
The text of ISO 10504:2013 has been prepared by Technical Committee ISO/TC 93 “Starch (including
derivatives and by-products)” of the International Organization for Standardization (ISO) and has been taken
over as EN ISO 10504:2015 by CCMC.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2016, and conflicting national standards shall be withdrawn at
the latest by January 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 10504:2000.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 10504:2013 has been approved by CEN as EN ISO 10504:2015 without any modification.
3
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SIST EN ISO 10504:2015
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SIST EN ISO 10504:2015
INTERNATIONAL ISO
STANDARD 10504
Second edition
2013-09-01
Starch derivatives — Determination
of the composition of glucose syrups,
fructose syrups and hydrogenated
glucose syrups — Method using high-
performance liquid chromatography
Produits dérivés de l’amidon — Détermination de la composition
des sirops de glucose, des sirops de fructose et des sirops de glucose
hydrogénés — Méthode par chromatographie en phase liquide à
haute performance
Reference number
ISO 10504:2013(E)
©
ISO 2013
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SIST EN ISO 10504:2015
ISO 10504:2013(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2013
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
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E-mail copyright@iso.org
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Published in Switzerland
ii © ISO 2013 – All rights reserved
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SIST EN ISO 10504:2015
ISO 10504:2013(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Principle . 1
4 Reagents . 1
5 Apparatus . 2
6 Procedure. 3
6.1 Choice of column . 3
6.2 System start-up . 3
6.3 Calibration of column . 3
6.4 Sample preparation . 4
6.5 Sample analysis . 4
7 Calculation . 4
8 Precision . 5
8.1 Repeatability . 5
8.2 Reproducibility . 5
Annex A (informative) Examples of standard solutions . 7
© ISO 2013 – All rights reserved iii
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SIST EN ISO 10504:2015
ISO 10504:2013(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directives
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received. www.iso.org/patents
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
The committee responsible for this document is ISO/TC 93, Starch (including derivatives and by-products).
This second edition cancels and replaces ISO 10504:1998, of which it constitutes a minor revision.
iv © ISO 2013 – All rights reserved
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SIST EN ISO 10504:2015
INTERNATIONAL STANDARD ISO 10504:2013(E)
Starch derivatives — Determination of the composition
of glucose syrups, fructose syrups and hydrogenated
glucose syrups — Method using high-performance liquid
chromatography
1 Scope
This International Standard describes a high-performance liquid chromatographic (HPLC) method
for measuring the composition of dextrose solutions, glucose syrups, fructose-containing syrups,
hydrogenated glucose syrups, sorbitol, mannitol and maltitol. The constituents are mainly glucose,
maltose, maltotriose, fructose, sorbitol, mannitol, maltitol and malto-oligosaccharides.
The use of a column packed with cation-exchange resin is essential.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the editi
...
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