SIST-TP CEN/TR 15641:2007

Food analysis - Determination of pesticide residues by LC-MS/MS - Tandem mass spectrometric parameters

This Technical Report lists mass spectrometric parameters which are useful for the application of European Standards for the determination of pesticide residues in foods of plant origin that use LC-MS/MS, such as the standards in preparation:
prEN 15637 "Foods of plant origin — Determination of pesticide residues using LC-MS/MS following methanol extraction and clean up using diatomaceous earth"
prEN 15662 "Foods of plant origin — Determination of pesticide residues using GC-MS and/or LC-MS/MS following acetonitrile extraction/partitioning by dispersive SPE — QuEChERS-method”
To facilitate the determination of active substances and/or metabolites using LC-MS/MS, Table 1 specifies the precursor ions and product ions suitable for quantification, which can be used independently of the type of triple quadrupole mass spectrometer. However, using an ion trap mass spectrometer other product ions can be generated or at least the relative intensities of the ions are different to triple quadrupole instruments. Furthermore, the additional parameters declustering potential (DP), collision energy (CE), relative retention times and an approximate classification of detection sensitivity are presented in Table 1. These were derived using the API 2000 ) and should be applicable at least for other instruments of the API type (Applied Biosystems).

Lebensmitteluntersuchung - Bestimmung von Pestizidrückständen mit LC-MS/MS - Parameter für die Tandem-Massenspektrometrie

Analyse des produits alimentaires - Détermination des résidus de pesticides par CL-SM/SM - Parametres de spectrométrie de masse en tandem

Analize živil - Določevanje ostankov pesticidov z LC-MS/MS - Parametri tandemske masne spektrometrije

General Information

• Status: Published
• Publication Date: 30-Sep-2007
• ICS: 67.050 - General methods of tests and analysis for food products
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 05-Sep-2007
• Due Date: 10-Nov-2007
• Completion Date: 01-Oct-2007
• Ref Project: CEN/TR 15641:2007 - Food analysis - Determination of pesticide residues by LC-MS/MS - Tandem mass spectrometric parameters

Overview

CEN/TR 15641:2007 is a CEN Technical Report that provides tandem mass spectrometric parameters to support LC‑MS/MS determination of pesticide residues in foods of plant origin. The document lists precursor and product ions (SRM transitions), declustering potentials (DP), collision energies (CE), relative retention times (RRT) and an approximate classification of detection sensitivity for hundreds of pesticides and metabolites. Parameters were derived on an Applied Biosystems API 2000 instrument and include example LC and MS operating conditions to aid transferability to other systems.

Key topics

• SRM transitions for LC‑MS/MS: Table entries specify precursor ions and two product ions recommended for quantification and confirmation (selected reaction monitoring).
• Instrument parameters: Declustering potential (DP), collision energy (CE), dwell times and focusing potentials are provided to optimize sensitivity for each analyte.
• Ionization and adducts: Guidance on ESI vs APCI, formation of [M+H]+, [M-H]- and common adducts (e.g., [M+NH4]+), and how adducts affect quantification.
• Method transferability: Practical notes on adapting DP/CE for other instruments (e.g., increase DP by +20 V for API 3000/4000) and on differences with ion trap vs triple quadrupole systems.
• LC and MS example conditions: Representative LC conditions (column type, mobile phases with ammonium formate, gradient, injection volume) and MS source settings (Turbo Ion Spray, gas temperatures/pressures, ion spray voltage) to support reproducible retention time (RRT) alignment.
• Confirmation requirements: Recommendation to record at least two SRM transitions per compound when LC‑MS/MS is used for both quantification and confirmation.

Applications

• Food testing laboratories: Routine multi‑residue analysis of fruits, vegetables, cereals and other plant‑origin foods using LC‑MS/MS.
• Method developers and validation teams: Starting point for tuning SRM parameters and transferring methods between instrument types.
• Regulatory and compliance labs: Supports implementation of European standards for pesticide residue monitoring and enforcement.
• Instrument vendors and service labs: Useful reference for instrument‑specific tuning and advice on expected sensitivity classes.

Who should use this standard

• Analytical chemists in food safety and environmental labs
• Quality assurance teams in food industry
• Regulatory agencies and accredited testing laboratories
• Developers of multi‑residue LC‑MS/MS methods and SOPs

Related standards

• prEN 15637 - Foods of plant origin: LC‑MS/MS following methanol extraction/diatomaceous earth cleanup
• prEN 15662 - Foods of plant origin: GC‑MS and/or LC‑MS/MS following QuEChERS (acetonitrile extraction/dispersive SPE)

CEN/TR 15641:2007 is a practical, instrument‑oriented reference for implementing LC‑MS/MS pesticide residue analysis, helping labs optimize SRM transitions, DP and CE settings, and align retention times for robust multi‑residue methods.

Standards Content (Sample)

SLOVENSKI STANDARD
01-november-2007
$QDOL]HåLYLO'RORþHYDQMHRVWDQNRYSHVWLFLGRY]/&06063DUDPHWULWDQGHPVNH
PDVQHVSHNWURPHWULMH
Food analysis - Determination of pesticide residues by LC-MS/MS - Tandem mass
spectrometric parameters
Lebensmitteluntersuchung - Bestimmung von Pestizidrückständen mit LC-MS/MS -
Parameter für die Tandem-Massenspektrometrie
Analyse des produits alimentaires - Détermination des résidus de pesticides par CL-
SM/SM - Parametres de spectrométrie de masse en tandem
Ta slovenski standard je istoveten z: CEN/TR 15641:2007
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

TECHNICAL REPORT
CEN/TR 15641
RAPPORT TECHNIQUE
TECHNISCHER BERICHT
August 2007
ICS 67.050
English Version
Food analysis - Determination of pesticide residues by LC-
MS/MS - Tandem mass spectrometric parameters
Analyse des produits alimentaires - Détermination des Lebensmitteluntersuchung - Bestimmung von
résidus de pesticides par CL-SM/SM - Paramètres de Pestizidrückständen mit LC-MS/MS - Parameter für die
spectrométrie de masse en tandem Tandem-Massenspektrometrie
This Technical Report was approved by CEN on 25 July 2007. It has been drawn up by the Technical Committee CEN/TC 275.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TR 15641:2007: E
worldwide for CEN national Members.

Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Analyte specific parameters for selective reaction monitoring of pesticides.5
2.1 General.5
2.2 LC Parameters.6
2.3 General MS/MS parameters .6
2.4 Analyte specific MS/MS Parameters.7
Bibliography .23

Foreword
This document (CEN/TR 15641:2007) has been prepared by Technical Committee CEN/TC 275 “Food
analysis - Horizontal methods”, the secretariat of which is held by DIN.
Introduction
Pesticide residue analysis employs multi methods involving extraction of residues from foods and clean up of
the extract to obtain as many pesticide residues as possible in the purified extracts. Afterwards the extracts
can be analysed by different kinds of instruments.
The hyphenation of liquid chromatography (LC) and tandem mass spectrometry (MS/MS) has become one of
the most universal, but selective and sensitive analysis techniques for identification and quantification of
pesticide residues in extracts of foods.
For the ionization of the analytes (M) in LC-MS/MS, electro spray ionization (ESI) or atmospheric pressure
chemical ionization (APCI) interfaces are most commonly used. Depending on the selected polarity of the
+ -
ionization, protonated or deprotonated molecular ions like [M+H] or [M-H] are generated. Using ESI,
relatively stable adducts (clusters) of the analytes (M) and components of the mobile phase like ions (e.g.
ammonium, sodium or potassium ions) or solvent molecules (e.g. methanol) can be additionally formed. To
obtain a high yield of quasi molecular ions and therefore to increase the sensitivity of the measurement these
clusters have to be broken. When using ESI the formed adducts are accelerated by a potential (e.g.
declustering potential or cone voltage) in the ion source at atmospheric pressure. Due to collision with neutral
gas molecules the clusters (adducts) are broken in the ion source. Under certain conditions some adduct-ions
+
formed are stable (e.g. with ammonium cation [M+NH ] ). It should be mentioned, however, that ammonium
adducts are usually not generated in the APCI-mode and that their formation when using ESI strongly
+
depends on the concentration of ammonium ions in the LC mobile phase. Adduct ions like [M+NH ] can also
be used alternatively for quantification if they were shown to provide reproducible signals. Sodium adducts are
usually not suitable for quantitative analysis as their formation and decomposition tends to be highly
irreproducible.
The selective determination of each target compound is performed by acquisition of characteristic product ions
of the precursor ion (quasi molecular ion or adduct) in the ‘selected reaction monitoring’ (SRM).
Decomposition of the precursor ions in the collision cell is induced by collision with molecules of the collision
gas (nitrogen or argon). The collision energy (CE) necessary for fragmentation is a very substantial parameter
of the MS/MS optimization for maximum sensitivity.
If LC-MS/MS measurement should be used not only for quantification but also for confirmation of positive
findings, at least two SRM transitions have to be recorded.
1 Scope
This Technical Report lists mass spectrometric parameters which are useful for the application of European
Standards for the determination of pesticide residues in foods of plant origin that use LC-MS/MS, such as the
standards in preparation:
prEN 15637 "Foods of plant origin — Determination of pesticide residues using LC-MS/MS following methanol
extraction and clean up using diatomaceous earth"
prEN 15662 "Foods of plant origin — Determination of pesticide residues using GC-MS and/or LC-MS/MS
following acetonitrile extraction/partitioning by dispersive SPE — QuEChERS-method”
To facilitate the determination of active substances and/or metabolites using LC-MS/MS, Table 1 specifies the
precursor ions and product ions suitable for quantification, which can be used independently of the type of
triple quadrupole mass spectrometer. However, using an ion trap mass spectrometer other product ions can
be generated or at least the relative intensities of the ions are different to triple quadrupole instruments.
Furthermore, the additional parameters declustering potential (DP), collision energy (CE), relative retention
times and an approximate classification of detection sensitivity are presented in Table 1. These were derived
)
using the API 2000 and should be applicable at least for other instruments of the API type (Applied
Biosystems).
2 Analyte specific parameters for selective reaction monitoring of pesticides
2.1 General
All values indicated in Table 2 were acquired using the above mentioned LC-MS/MS system under the
experimental conditions as outlined in 2.2. Comparative investigations showed that these parameters can be
transferred simply on instruments of other types of the same manufacturer or after adjustment also on devices
of other manufacturers (see in 2.3)

)
Instruments of the API type are products supplied by Applied Biosystems (Foster City, CA, USA). This information is
given for the convenience of users of this Technical Report and does not constitute an endorsement by CEN of the
product named. Equivalent products may be used if they can be shown to lead to the same results.
2.2 LC Parameters
The following LC operating conditions have been proven to be satisfactory. This is an example for appropriate
experimental conditions. Equivalent conditions may be used if they can be shown to lead to the same results.
® )
HPLC pump HP1100 Binary Pump (G1312A) ®
Autosampler HP1100 (G1313A)
Injection volume 20 µl
® )
Column Phenomenex Aqua 5 µ C18 125 Å, 50 mm × 2 mm
Mobile phase A Methanol/water 2+8 (V/V) with 5 mmol/l ammonium formate
Mobile phase B Methanol/water 9+1 (V/V) with 5 mmol/l ammonium formate
Flow rate 0,2 ml/min
Column temperature 20 °C
Gradient Linear:
0 min 0 % B
11 min 100 % B
23 min 100 % B
25 min  0 % B
36 min 0 % B (equilibration time)
As slight fluctuations in the measurement conditions influence the retention time, usually relative retention
times (RRT), related to a standard substance, are compared. The standard substance for the calculation of
the RRT values in Table 1 was Imazalil (RRT = 1,000).
It could be shown that the use of other mobile phase systems does not shift the order of elution substantially,
except for those compounds which are sensitive to the pH of eluent. Often retention data can be transferred to
HPLC columns of other manufacturers, if a typical reversed phase is used.
2.3 General MS/MS parameters
The following general MS/MS operating conditions have been proven to be satisfactory. This is an example for
appropriate experimental conditions. Equivalent conditions may be used if they can be shown to lead to the
same results ®
MS/MS instrument Applied Biosystems API 2000 ®
Ion source Turbo Ion Spray (ESI)

)
Instruments of the HP type are products supplied by Agilent Technologies Inc. (Palo Alto, CA, USA). This information is
given for the convenience of users of this Technical Report and does not constitute an endorsement by CEN of the
product named. Equivalent products may be used if they can be shown to lead to the same results.
)
HPLC columns of the Aqua type are products supplied by Phenomenex (Torrance, CA, USA). This information is given
for the convenience of users of this Technical Report and does not constitute an endorsement by CEN of the product
named. Equivalent products may be used if they can be shown to lead to the same results.
Table 1 — Ion source and general parameters
Ion polarity positive Gas 2 temperature 400 °C
Curtain gas nitrogen, 35 psi (241 kPa) Resolution MS 1 unit
Collision gas nitrogen, 2 arbitrary units Resolution MS 2 unit
Ion spray voltage 5500 V Dwell time 25 ms
Gas 1 nitrogen, 60 psi (414 kPa) Focusing potential 360 V
Gas 2 nitrogen, 60 psi (414 kPa)

2.4 Analyte specific MS/MS Parameters
The analyte specific parameters for selective reaction monitoring of pesticides are listed in Table 1 [1]. The
names of the individual analytes are supplemented by the CAS number (Chemical Abstracts Service), which
is useful for the search in databases. It is usually taken from [2], but there can be several numbers in
individual cases, e.g. for isomers and racemates. ®
The values for the declustering potential (DP), indicated in Table 1 for the API 2000 , have to be increased by
® ®
20 V for tandem mass spectrometers of the type API 3000 or API 4000 . It is to be considered that DP
breaks not only the clusters but can already induce fragmentation of the precursor ions (at too high values) in
the ESI source before entering into the first MS.
® ®
NOTE It is not necessary to change the collision energy for API 3000 or API 4000 instruments as the differences for
the CE are less than 5 V.
When using tandem mass spectrometers of other manufacturers the correct value for the collision energy
should be determined in tuning experiments for analytes with particularly low and high collision energy since it
has relevant influence on the intensity of the SRM transition. Based on these data, in a first approximation the
values for the collision energy of further pesticides can be derived proportionally from the data in the table and ®
the observed difference (difference of CE at instrument X to the API 2000 ). The values of the declustering
potential (other name “cone voltage”) for other instruments have to be determined individually. On the intensity
of the SRM transitions this parameter has a smaller influence than the collision energy.
Table 2 — MS/MS Parameters of 497 analytes
st nd
Pesticide (Metabolite) 1 SRM 2 SRM

2,4-D 94-75-7 ESI - [M-H]- 219,0 -21 160,9 -14 124,9 -34 0,69 ***
2,4-DB 94-82-6 ESI - [M-H]- 247,0 -66 160,8 -12 124,9 -34 0,86 ***
2-Naphthyloxyacetic acid 120-23-0 ESI +[M+NH4]+ 220,1 36 157,1 19 127,1 43 0,66 n.a.
2-Naphthyloxyacetic acid 120-23-0 ESI - [M-H]- 201,1 -71 143,0 -18 114,9 -50 0,66 ***
3,4,5-Trimethacarb 2686-99-9 ESI +[M+H]+ 194,1 61 137,1 15 122,0 35 0,86 ****
4-CPA 122-88-3 ESI - [M-H]- 185,0 -71 126,8 -18 140,7 -12 0,47 **
Acephate 30560-19-1 ESI +[M+H]+ 184,1 6 124,9 25 142,9 13 0,11 ****
Acetamiprid 135410-20-7 ESI +[M+H]+ 223,0 36 126,0 27 90,1 45 0,58 ****
CAS No.
Ionization
Quasi molecular ion
Q1 Mass (amu)
Declustering potential (V)
Q3 Mass (amu)
Collision energy (V)
Q3 Mass (amu)
Collision energy (V)
Relative retention on
a
endcapped RP phase
)
b
Sensitivity of detection
st nd
Pesticide (Metabolite) 1 SRM 2 SRM

Acibenzolar-S-methyl 135158-54-2 ESI +[M+H]+ 210,9 26 136,1 39 140,0 31 0,92 **
Aclonifen 74070-46-5 ESI +[M+H]+ 265,0 56 182,1 39 218,0 33 0,99 **
Acrinathrin 101007-06-1 ESI +[M+NH4]+ 559,1 26 208,1 23 181,1 43 1,20 *
Alachlor 15972-60-8 ESI +[M+H]+ 270,1 31 238,1 15 162,2 25 0,97 ****
Aldicarb 116-06-3 ESI +[M+NH4]+ 208,1 1 89,1 21 116,0 13 0,66 ****
Aldicarb-sulfoxide 1646-87-3 ESI +[M+H]+ 207,1 36 89,1 17 131,9 11 0,15 ****
Aldoxycarb 1646-88-4 ESI +[M+NH4]+ 240,1 11 148,0 19 86,1 27 0,19 ****
Alloxydim 55634-91-8 ESI +[M+H]+ 324,2 11 178,3 27 234,2 19 0,77 ****
Ametryn 834-12-8 ESI +[M+H]+ 228,1 36 186,2 25 96,1 35 0,90 ****
Amidosulfuron 120923-37-7 ESI +[M+H]+ 370,0 21 217,9 31 260,9 19 0,46 ****
Aminocarb 2032-59-9 ESI +[M+H]+ 209,1 16 152,1 19 137,2 31 0,74 ****
Amitraz 33089-61-1 ESI +[M+H]+ 294,2 16 163,1 21 122,1 41 1,19 ****
Amitrole 61-82-5 ESI +[M+H]+ 85,0 51 58,2 29 57,0 23 0,07 **
Aramit 140-57-8 ESI +[M+NH4]+ 352,1 41 191,2 19 105,0 57 1,09 ****
Atrazine 1912-24-9 ESI +[M+H]+ 216,1 21 174,0 25 103,9 27 0,83 ****
Atrazine, 2-hydroxy- 2163-68-0 ESI + [M+H]+ 198,1 66 69,0 47 156,2 25 0,65 ****
Atrazine, desethyl- 6190-65-4 ESI +[M+H]+ 188,1 56 104,0 33 146,0 25 0,59 ***
Atrazine, desethyl-2-hydroxy- 6190-65-4 ESI + [M+H]+ 170,1 66 128,1 23 86,0 31 0,14 ****
Atrazine, desisopropyl- 1007-28-9 ESI + [M+H]+ 174,1 56 104,2 31 96,0 27 0,39 ***
Avermectin B1a 65195-55-3 ESI +[M+NH4]+ 890,5 41 305,1 35 145,2 43 1,33 ***
Avermectin B1b 65195-56-4 ESI +[M+NH4]+ 876,5 41 291,1 35 145,2 43 1,26 ***
Azaconazole 60207-31-0 ESI +[M+H]+ 300,0 56 231,0 23 159,0 37 0,86 ****
Azamethiphos 35575-96-3 ESI +[M+H]+ 325,0 16 183,0 21 139,2 33 0,74 ****
Azimsulfuron 120162-55-2 ESI +[M+H]+ 425,1 31 182,1 23 156,1 43 0,55 ***
Azinphos-ethyl 2642-71-9 ESI +[M+H]+ 346,0 26 132,2 21 160,2 15 0,96 ****
Azinphos-methyl 86-50-0 ESI +[M+H]+ 318,0 16 132,2 21 160,2 13 0,89 ****
Azocyclotin 41083-11-8 ESI +[M-OH]+ 369,2 76 204,8 23 287,0 17 n.a. n.a.
Azoxystrobin 131860-33-8 ESI +[M+H]+ 404,1 36 371,9 19 343,9 29 0,90 ****
Beflubutamid 113614-08-7 ESI +[M+NH4]+ 373,1 26 91,2 47 162,1 39 1,00 ***
Benalaxyl 71626-11-4 ESI +[M+H]+ 326,2 26 148,2 27 208,2 21 1,01 ****
Bendiocarb 22781-23-3 ESI +[M+H]+ 224,1 6 167,2 13 108,9 21 0,76 ****
Benfuracarb 82560-54-1 ESI +[M+H]+ 411,2 1 195,1 31 252,0 19 1,05 ****
Benomyl 17804-35-2 ESI +[M+H]+ 291,1 16 160,1 35 192,2 17 1,03 **
Bensulfuron-methyl 83055-99-6 ESI +[M+H]+ 411,1 51 148,9 27 119,0 51 0,85 ***
CAS No.
Ionization
Quasi molecular ion
Q1 Mass (amu)
Declustering potential (V)
Q3 Mass (amu)
Collision energy (V)
Q3 Mass (amu)
Collision energy (V)
Relative retention on
a
endcapped RP phase
)
b
Sensitivity of detection
st nd
Pesticide (Metabolite) 1 SRM 2 SRM

Bentazone 25057-89-0 ESI - [M-H]- 239,1 -51 132,0 -32 197,0 -24 0,33 ***
Benzoximate 29104-30-1 ESI +[M+H]+ 364,1 1 199,1 17 105,1 35 1,02 ****
Bifenox 42576-02-3 ESI +[M+NH4]+ 358,9 6 309,9 17 189,1 35 1,04 **
Bifenthrin 82657-04-3 ESI +[M+NH4]+ 440,1 36 181,2 21 166,2 55 1,33 ****
Binapacryl 485-31-4 ESI +[M+NH4]+ 340,1 26 83,2 21 54,9 63 1,23 *
Bioresmethrin 28434-01-7 ESI +[M+NH4]+ 356,2 21 171,2 21 128,1 53 1,23 n.a.
Bitertanol 55179-31-2 ESI +[M+H]+ 338,2 1 70,0 25 269,2 15 1,02 ***
Boscalid 188425-85-6 ESI +[M+H]+ 343,0 71 307,0 27 139,9 27 0,92 ****
Bromacil 314-40-9 ESI +[M+H]+ 261,0 21 205,0 19 187,9 37 0,75 ***
Bromophos-ethyl 4824-78-6 ESI +[M+H]+ 394,9 51 338,7 23 366,9 17 1,20 **
Bromoxynil 1689-84-5 ESI -[M-H]- 273,9 -46 79,0 -36 80,9 -40 0,56 ***
Bromuconazole 116255-48-2 ESI +[M+H]+ 378,0 46 159,0 37 69,9 35 0,95 ***
Bupirimate 41483-43-6 ESI +[M+H]+ 317,1 31 166,1 33 108,1 35 0,98 ****
Buprofezin 69327-76-0 ESI +[M+H]+ 306,2 6 201,2 17 116,2 21 1,09 ****
Butafenacil 134605-64-4 ESI +[M+NH4]+ 492,1 36 180,1 59 331,0 29 0,95 **
Butocarboxim 34681-10-2 ESI +[M+NH4]+ 208,1 1 116,1 11 75,0 15 0,66 ****
Butocarboxim-sulfoxide 34681-24-8 ESI +[M+H]+ 207,1 6 131,9 11 75,0 19 0,13 ****
Butoxycarboxim 34681-23-7 ESI +[M+NH4]+ 240,1 6 106,0 19 166,0 13 0,18 **
Buturon 3766-60-7 ESI +[M+H]+ 237,1 41 84,1 21 126,1 37 0,85 n.a.
Butylate 2008-41-5 ESI +[M+H]+ 218,2 66 57,1 29 156,2 17 1,07 ***
Cadusafos 95465-99-9 ESI +[M+H]+ 271,1 66 159,0 19 97,0 47 1,05 ***
Carbaryl 63-25-2 ESI +[M+H]+ 202,1 11 144,9 15 127,0 35 0,79 ****
Carbendazim 10605-21-7 ESI +[M+H]+ 192,1 41 160,0 25 132,0 41 0,64 ****
Carbetamide 16118-49-3 ESI +[M+H]+ 237,1 21 118,1 19 192,0 13 0,72 ****
Carbofuran 1563-66-2 ESI +[M+H]+ 222,1 16 165,1 17 123,0 29 0,75 ****
Carbofuran, 3-hydroxy- 16655-82-6 ESI +[M+H]+ 238,1 21 181,1 15 163,1 19 0,56 ***
Carbosulfan 55285-14-8 ESI +[M+H]+ 381,2 36 118,1 25 160,2 21 1,31 ***
Carboxin 5234-68-4 ESI +[M+H]+ 236,1 26 142,9 21 86,9 33 0,78 ****
Carfentrazone-ethyl 128639-02-1 ESI +[M+H]+ 412,0 66 365,9 25 345,9 31 0,99 ***
Cartap hydrochloride 15263-52-2 ESI + [M+H]+ 238,1 26 73,0 37 150,1 19 0,14 **
Chinomethionat 2439-01-2 ESI +[M+H]+ 234,9 41 207,1 21 163,0 39 0,72 *
Chlorbromuron 13360-45-7 ESI +[M+H]+ 292,9 51 182,1 23 204,0 21 0,92 ***
Chlorbufam 1967-16-4 ESI +[M+NH4]+ 241,0 6 172,1 17 154,1 27 0,91 ***
Chlorfenvinphos 470-90-6 ESI +[M+H]+ 358,9 36 155,0 19 99,2 43 1,00 ****
CAS No.
Ionization
Quasi molecular ion
Q1 Mass (amu)
Declustering potential (V)
Q3 Mass (amu)
Collision energy (V)
Q3 Mass (amu)
Collision energy (V)
Relative retention on
a
endcapped RP phase
)
b
Sensitivity of detection
st nd
Pesticide (Metabolite) 1 SRM 2 SRM

Chlorfluazuron 71422-67-8 ESI +[M+H]+ 539,9 71 158,0 27 383,0 27 1,19 ***
Chlorfluazuron 71422-67-8 ESI - [M-H]- 538,0 -36 517,9 -18 354,9 -28 1,18 n.a.
Chloridazon 1698-60-8 ESI +[M+H]+ 222,0 56 92,2 35 104,2 31 0,58 ****
Chlorimuron-ethyl 90982-32-4 ESI +[M+H]+ 415,0 46 121,1 53 186,1 27 0,76 ***
Chlormephos 24934-91-6 ESI +[M+H]+ 234,9 31 97,0 33 143,0 19 0,98 *
Chlorobenzilate 510-15-6 ESI +[M+NH4]+ 342,0 21 307,1 17 139,1 47 1,05 *
Chlorotoluron 15545-48-9 ESI +[M+H]+ 213,1 36 72,0 33 140,0 33 0,83 ****
Chloroxuron 1982-47-4 ESI +[M+H]+ 291,1 51 72,0 41 218,1 33 0,95 ****
Chlorpyrifos 2921-88-2 ESI +[M+H]+ 349,9 21 96,9 41 198,0 25 1,13 ***
Chlorpyrifos-methyl 5598-13-0 ESI +[M+H]+ 321,9 26 125,1 27 289,8 25 1,04 **
Chlorsulfuron 64902-72-3 ESI +[M+H]+ 358,0 51 141,0 23 167,0 25 0,59 ****
Chlorthiamid 1918-13-4 ESI +[M+H]+ 205,9 36 118,9 55 154,0 45 0,57 **
Chlorthiophos 60238-56-4 ESI +[M+H]+ 360,9 41 305,0 21 333,0 17 1,16 ****
Cinidon-ethyl 142891-20-1 ESI +[M+NH4]+ 411,1 41 348,0 29 107,2 45 1,11 ***
Cinosulfuron 94593-91-6 ESI +[M+H]+ 414,1 36 182,9 23 214,9 21 0,62 ****
Clethodim 99129-21-2 ESI +[M+H]+ 360,1 46 268,2 17 164,0 25 0,92 **
Clethodim 99129-21-2 ESI - [M-H]- 358,1 -66 238,1 -30 no 0,85 n.a.
Clethodim-imin-sulfon not available ESI + [M+H]+ 302,1 71 98,0 41 208,1 27 0,58 n.a.
Clethodim-imin-sulfoxid not available ESI +[M+H]+ 286,1 26 208,1 21 166,0 31 0,57 n.a.
Clethodim-sulfon not available ESI +[M+H]+ 392,1 1 164,2 33 208,1 27 0,67 n.a.
Clethodim-sulfon, 5-hydroxy- not available ESI - [M-H]- 406,1 -61 92,8 -30 64,1 -80 0,18 n.a.
Clethodim-sulfon, 5-Hydroxy- not available ESI + [M+H]+ 408,1 16 204,0 27 176,1 35 0,55 n.a.
Clethodim-sulfoxid not available ESI +[M+H]+ 376,1 1 206,0 19 164,1 29 0,67 n.a.
Clodinafop-propargyl 105512-06-9 ESI +[M+H]+ 350,0 41 266,1 21 91,2 41 0,98 ****
Clofentezine 74115-24-5 ESI +[M+H]+ 303,1 56 102,1 47 138,1 21 1,02 ***
Clomazone 81777-89-1 ESI +[M+H]+ 240,1 26 125,0 27 89,1 65 0,88 ****
Clopyralid 1702-17-6 ESI +[M+H]+ 191,9 36 146,0 29 110,1 47 0,10 **
Cloquintocet-mexyl 99607-70-2 ESI +[M+H]+ 336,1 16 192,2 37 238,1 21 1,11 ****
Clothianidin 210880-92-5 ESI +[M+H]+ 250,0 76 169,1 19 132,1 19 0,51 ***
Coumaphos 56-72-4 ESI +[M+H]+ 363,0 66 227,0 35 307,0 23 1,01 ****
Crotoxyphos 7700-17-6 ESI +[M+NH4]+ 332,1 1 127,1 33 211,0 15 0,92 ****
Cyanazine 21725-46-2 ESI +[M+H]+ 241,1 41 214,1 23 104,1 41 0,73 ***
Cyanofenphos 13067-93-1 ESI +[M+H]+ 304,0 46 276,0 17 157,1 27 1,00 **
Cyanophos 2636-26-2 ESI +[M+NH4]+ 261,0 6 125,0 27 212,0 27 0,84 *
CAS No.
Ionization
Quasi molecular ion
Q1 Mass (amu)
Declustering potential (V)
Q3 Mass (amu)
Collision energy (V)
Q3 Mass (amu)
Collision energy (V)
Relative retention on
a
endcapped RP phase
)
b
Sensitivity of detection
st nd
Pesticide (Metabolite) 1 SRM 2 SRM

Cyazofamid 120116-88-3 ESI +[M+H]+ 325,0 36 108,0 19 261,0 15 0,97 ****
Cyclanilide 113136--77-9 ESI - [M-H]- 272,0 -56 159,9 -28 228,0 -14 0,82 ****
Cycloate 1134-23-2 ESI +[M+H]+ 216,1 21 154,3 17 134,2 19 1,05 **
Cycloxydim 101205-02-1 ESI +[M+H]+ 326,2 51 280,0 17 180,0 25 0,93 **
Cycloxydim 101205-02-1 ESI - [M-H]- 324,2 -61 235,9 -68 133,8 -68 0,84 n.a.
Cyfluthrin 68359-37-5 ESI +[M+NH4]+ 451,1 26 191,0 21 127,0 41 1,16 **
Cyhalofop-butyl 122008-85-9 ESI +[M+NH4]+ 375,1 31 256,1 21 120,0 41 1,05 *
Cyhexatin 13121-70-5 ESI +[M-OH]+ 369,2 76 204,8 23 287,0 17 n.a. n.a.
Cymoxanil 57966-95-7 ESI +[M+H]+ 199,1 46 128,0 13 110,9 25 0,61 ****
Cypermethrin 52315-07-8 ESI +[M+NH4]+ 433,1 1 191,0 21 127,0 39 1,16 **
Cyphenothrin[(1R)-trans- 39515-40-7 ESI +[M+NH4]+ 393,2 31 151,2 21 123,1 33 1,21 **
isomers]
Cyproconazole 113096-99-4 ESI +[M+H]+ 292,1 16 70,2 35 125,1 35 0,93 ***
Cyprodinil 121552-61-2 ESI +[M+H]+ 226,1 61 76,9 63 92,9 45 1,01 ***
Cyromazine 66215-27-8 ESI +[M+H]+ 167,1 46 125,0 25 108,1 29 0,15 ***
Daminozide 1596-84-5 ESI +[M+H]+ 161,1 46 142,9 15 61,0 19 0,06 **
Deltamethrin 52918-63-5 ESI +[M+NH4]+ 522,9 16 280,7 23 181,3 51 1,20 ***
Demeton-S-methyl 919-86-8 ESI +[M+NH4]+ 248,0 6 89,1 17 61,0 47 0,77 ****
Demeton-S-methyl sulfon 17040-19-6 ESI +[M+H]+ 263,0 71 108,9 37 169,0 21 0,30 ****
Desmedipham 13684-56-5 ESI +[M+NH4]+ 318,1 31 182,2 19 136,0 33 0,87 ****
Dialifos 10311-84-9 ESI +[M+H]+ 394,0 36 208,1 23 186,9 17 1,04 n.a.
Di-allate 2303-16-4 ESI +[M+H]+ 270,0 41 86,1 23 108,8 37 1,05 ****
Diazinon 333-41-5 ESI +[M+H]+ 305,1 21 169,1 29 96,6 41 1,01 ****
Dicamba 1918-00-9 ESI - [M-H]- 219,0 -21 175,0 -6 144,8 -14 0,27 *
Dichlofenthion 97-17-6 ESI +[M+H]+ 314,9 26 258,9 21 286,9 15 1,00 **
Dichlofluanid 1085-98-9 ESI +[M+NH4]+ 350,0 21 123,0 41 223,9 21 0,93 ***
Dichlorprop-P 15165-67-0 ESI - [M-H]- 233,0 -21 161,0 -14 125,1 -36 0,78 ***
Dichlorvos 62-73-7 ESI +[M+H]+ 220,9 26 127,1 27 108,9 25 0,74 ***
Diclobutrazol 75736-33-3 ESI +[M+H]+ 328,1 21 70,1 39 159,0 45 0,99 ****
Diclofop-methyl 51338-27-3 ESI +[M+NH4]+ 358,0 26 281,0 21 120,0 39 1,09 ****
Dicloran 99-30-9 ESI - [M-H]- 205,0 -66 175,0 -20 168,9 -24 0,88 *
Dicrotophos 3735-78-3 ESI +[M+H]+ 238,1 16 127,1 23 112,1 17 0,45 ****
Diethofencarb 87130-20-9 ESI +[M+H]+ 268,1 31 226,1 15 180,1 23 0,90 ****
Difenoconazole 119446-68-3 ESI +[M+H]+ 406,1 41 250,9 37 337,0 23 1,04 ****
Difenoxuron 14214-32-5 ESI +[M+H]+ 287,1 36 123,1 25 72,1 39 0,87 n.a.
CAS No.
Ionization
Quasi molecular ion
Q1 Mass (amu)
Declustering potential (V)
Q3 Mass (amu)
Collision energy (V)
Q3 Mass (amu)
Collision energy (V)
Relative retention on
a
endcapped RP phase
)
b
Sensitivity of detection
st nd
Pesticide (Metabolite) 1 SRM 2 SRM

Diflubenzuron 35367-38-5 ESI - [M-H]- 309,0 -36 155,9 -12 288,9 -8 1,00 ***
Diflufenican 83164-33-4 ESI +[M+H]+ 395,1 56 265,8 33 246,1 45 1,04 ***
Dimefuron 34205-21-5 ESI +[M+H]+ 339,1 61 167,0 29 256,0 21 0,88 ***
Dimethachlor 50563-36-5 ESI +[M+H]+ 256,1 26 224,2 19 148,1 33 0,86 ****
Dimethenamide 87674-68-8 ESI +[M+H]+ 276,1 11 244,1 19 168,1 33 0,90 ****
Dimethoate 60-51-5 ESI +[M+H]+ 230,0 11 125,0 29 198,8 13 0,55 ****
Dimethomorph 110488-70-5 ESI +[M+H]+ 388,1 46 301,1 27 165,0 43 0,92 ****
Dimetilan 644-64-4 ESI +[M+H]+ 241,1 66 72,1 25 196,0 15 0,59 n.a.
Diniconazole 83657-24-3 ESI +[M+H]+ 326,0 56 70,0 45 159,0 39 1,04 ***
Dinoseb 88-85-7 ESI - [M-H]- 239,1 -36 134,0 -52 193,0 -30 0,84 ***
Dinoterb 1420-07-1 ESI - [M-H]- 239,1 -21 207,0 -32 176,0 -48 0,86 ****
Dioxathion 78-34-2 ESI +[M+NH4]+ 474,0 31 271,0 19 97,1 61 1,09 ****
Diphenylamine 122-39-4 ESI +[M+H]+ 170,1 21 93,1 37 151,9 37 0,93 **
Disulfoton 298-04-4 ESI +[M+H]+ 275,0 6 89,2 17 61,1 43 1,04 ***
Dithianon 3347-22-6 ESI - [M-H]-. 296,0 -49 264,0 -24 238,0 -24 0,95 n.a.
Diuron 330-54-1 ESI +[M+H]+ 233,0 66 72,0 31 159,9 33 0,86 ****
DNOC 534-52-1 ESI - [M-H]- 197,0 -26 137,0 -24 108,8 -28 0,56 ***
Dodemorph 1593-77-7 ESI +[M+H]+ 282,3 51 116,1 29 98,2 39 1,21 ****
Edifenphos 17109-49-8 ESI +[M+NH4]+ 328,0 1 109,0 45 283,0 23 1,00 ***
Endosulfansulfat 1031-07-8 ESI -[M-H]- 420,8 -66 96,8 -38 79,9 -130 1,02 ***
EPN 2104-64-5 ESI +[M+H]+ 324,0 46 156,9 29 296,1 19 1,04 **
Epoxiconazole 106325-08-0 ESI +[M+H]+ 330,1 36 121,0 27 101,2 63 0,97 ****
EPTC 759-94-4 ESI +[M+H]+ 190,1 61 128,1 15 86,1 19 0,98 ***
Esfenvalerate 66230-04-4 ESI +[M+NH4]+ 437,1 41 167,2 23 125,0 51 1,19 *
Ethametsulfuron-methyl 97780-06-8 ESI +[M+H]+ 411,1 31 196,1 23 168,1 39 0,68 ****
Ethidimuron 30043-49-3 ESI +[M+H]+ 265,0 81 208,2 19 114,1 25 0,53 n.a.
Ethiofencarb 29973-13-5 ESI +[M+H]+ 226,1 16 107,2 21 164,0 13 0,80 ***
Ethiofencarb-sulfone 53380-23-7 ESI +[M+NH4]+ 275,3 11 106,9 25 201,0 15 0,45 ****
Ethiofencarb-sulfoxide 53380-22-6 ESI +[M+H]+ 242,1 41 107,1 23 185,0 13 0,47 ****
Ethion 563-12-2 ESI +[M+H]+ 385,0 16 199,1 17 171,0 23 1,11 ****
Et
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