SIST ISO 11261:1996

Soil quality - Determination of total nitrogen - Modified Kjeldahl method

Specifies a method for the determination of the total nitrogen (ammonium-N, nitrate-N, nitrite-N and organic N) content of a soil. Nitrogen in N-N-linkages, N-O-linkages and some heterocyclics (especially pyridine) is only partially determined. Applicable to all types of soils.

Qualité du sol - Dosage de l'azote total - Méthode de Kjeldahl modifiée

La présente Norme internationale prescrit une méthode de dosage de la teneur totale en azote dans le sol (sous sa forme ammonium, nitrate, nitrite et organique). L'azote dans les liaisons N-N et N-O, et dans certains hétérocycles (telle que la pyridine) n'est que partiellement dosé. La présente Norme internationale est applicable à tous les types de sol.

Kakovost tal - Določevanje celotnega dušika - Modificirana Kjeldahlova metoda

General Information

• Status: Published
• Publication Date: 30-Sep-1996
• ICS: 13.080.10 - Chemical characteristics of soils
• Technical Committee: KAT - Soil quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 01-Oct-1996
• Due Date: 01-Oct-1996
• Completion Date: 01-Oct-1996
• Directive: TP024 - Pravilnik o obratovalnem monitoringu pri vnosu nevarnih snovi in rastlinskih hranil v tla
• Directive: TP168 - Pravilnik o monitoringu kakovosti tal
• Directive: TP248 - Pravilnik o načinu vzorčenja, zagotavljanju kakovosti in metodologiji laboratorijskega analiziranja organske snovi in hranil na kmetijskih zemljiščih za namen spremljanja emisij in odvzemov toplogrednih plinov v kmetijstvu
• Directive: TP255 - Pravilnik o obratovalnem monitoringu stanja tal
• Ref Project: ISO 11261:1995 - Soil quality — Determination of total nitrogen — Modified Kjeldahl method

Standards Content (Sample)

SLOVENSKI STANDARD
SIST ISO 11261:1996
01-oktober-1996
Kakovost tal - Določevanje celotnega dušika - Modificirana Kjeldahlova metoda
Soil quality - Determination of total nitrogen - Modified Kjeldahl method
Qualité du sol - Dosage de l'azote total - Méthode de Kjeldahl modifiée
Ta slovenski standard je istoveten z: ISO 11261:1995
ICS:
13.080.10 Kemijske značilnosti tal Chemical characteristics of
soils
SIST ISO 11261:1996 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST ISO 11261:1996

---------------------- Page: 2 ----------------------
SIST ISO 11261:1996
INTERNATIONAL
ISO
STANDARD 11261
First edition
1995-03-01
Soil quality - Determination of total
nitrogen - Modified Kjeldahl method
Quake du so/
- Dosage de I ’azo te total - Methode de Kjeldahl modifiee
Reference number
ISO 7 1261: 1995(E)

---------------------- Page: 3 ----------------------
SIST ISO 11261:1996
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation sf national Standards bodies (ISO member bodies). The work
of preparing International Standards is normally carried out through ISO
technical committees. Esch member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard ISO 11261 was prepared by Technical Committee
lSO/TC 190, Seil quality, Subcommittee SC 3, Chemical methods and seil
charac teris tics.
Annex A of this International Standard is for information only.
0 ISO 1995
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronrc or mechanical, rncluding photocopyrng and
microfilm, without Permission in writing from the publisher.
International Organization for Standardization
Case Postale 56 l CH-l 211 Geneve 20 l Switzerland
Printed in Switzerland
ii

---------------------- Page: 4 ----------------------
SIST ISO 11261:1996
ISO 11261:1995(E)
INTERNATIONAL STANDARD 0 ISO
Soil quality - Determination of total nitrogen -
Modified Kjeldahl method
3 Principle
1 Scope
The method is based on the Kjeldahl-digestion, but
This International Standard specifies a method for the
titanium dioxide (TiO,) is used as the catalyst instead
determination of the total nitrogen (ammonium-N,
of selenium.
nitrate-N, nitrite-N and organic N) content of a soil.
Nitrogen in N-N-linkages, N-0-linkages and some het-
NOTE 1 Titanium dioxide is ecotoxicologically less
erocyclics (especially pyridine) is only partially deter-
harmfull than selenium.
mined. This International Standard is applicable to all
types of soils.
4 Reagents
All reagents shall be of recognized analytical grade.
Use water of grade 2 complying with ISO 3696.
4.1 Salicylic acid/sulfuric acid.
2 Normative references
Dissolve 25 g of salicylic acid in 1 litre of concentrated
sulfuric acid Q = 1,84 g/cm3).
The following Standards contain provisions which, (
through reference in this text, constitute provisions
4.2 Potassium sulfate catalyst mixture.
of this International Standard. At the time of publi-
cation, the editions indicated were valid. All Standards
Grind and thoroughly mix 200 g of potassium sulfate,
are subject to revision, and Parties to agreements
6 g of copper(ll) sulfate pentahydrate and 6 g of
based on this International Standard are encouraged
titanium dioxide, with the crystal structure of anatase.
to investigate the possibility of applying the most re-
cent editions of the Standards indicated below.
4.3 Sodium thiosulfate pentahydrate.
Members of IEC and ISO maintain registers of cur-
rently valid International Standards.
Crush the crystals to form a powder that Passes
through a sieve with an aperture of 0,25 mm.
ISO 3696: 1987, Water for analytical laboratory use -
Specifica tion and tes t me thods.
4.4 Sodium hydroxide, c(NaOH) = 10 mol/l.
ISO 5725:1986, Precision of test methods - Deter-
mina tion of repeatability and reproducibility for a
4.5 Boric acid Solution, p(H,BO,) = 20 g/l.
Standard test method by inter-laboratory tests.
4.6 Mixed indicator.
ISO 11464: 1994, Soil quality - Pretreatmen t of sam-
ples for physico-Chemical analyses.
Dissolve 0,l g of bromocresol green and 0,02 g of
methyl red in 100 ml of ethanol.
ISO 11465: 1993, Soil quality - Determination of dry
matter and water content on a mass basis -
4.7 Sulfuric acid, c(H+) = 0,Ol mol/l.
Gravimetric method.
7

---------------------- Page: 5 ----------------------
SIST ISO 11261:1996
0 ISO
ISO 11261:1995(E)
under the condenser of the distillation apparatus in
5 Apparatus
such a way that the end of the condenser dips into
the Solution. Add 20 ml of sodium hydroxide (4.4) to
Usual laboratory equipment and
the funnel of the apparatus and run the alkali slowly
into t
...

INTERNATIONAL
ISO
STANDARD 11261
First edition
1995-03-01
Soil quality - Determination of total
nitrogen - Modified Kjeldahl method
Quake du so/
- Dosage de I ’azo te total - Methode de Kjeldahl modifiee
Reference number
ISO 7 1261: 1995(E)

---------------------- Page: 1 ----------------------
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation sf national Standards bodies (ISO member bodies). The work
of preparing International Standards is normally carried out through ISO
technical committees. Esch member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard ISO 11261 was prepared by Technical Committee
lSO/TC 190, Seil quality, Subcommittee SC 3, Chemical methods and seil
charac teris tics.
Annex A of this International Standard is for information only.
0 ISO 1995
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronrc or mechanical, rncluding photocopyrng and
microfilm, without Permission in writing from the publisher.
International Organization for Standardization
Case Postale 56 l CH-l 211 Geneve 20 l Switzerland
Printed in Switzerland
ii

---------------------- Page: 2 ----------------------
ISO 11261:1995(E)
INTERNATIONAL STANDARD 0 ISO
Soil quality - Determination of total nitrogen -
Modified Kjeldahl method
3 Principle
1 Scope
The method is based on the Kjeldahl-digestion, but
This International Standard specifies a method for the
titanium dioxide (TiO,) is used as the catalyst instead
determination of the total nitrogen (ammonium-N,
of selenium.
nitrate-N, nitrite-N and organic N) content of a soil.
Nitrogen in N-N-linkages, N-0-linkages and some het-
NOTE 1 Titanium dioxide is ecotoxicologically less
erocyclics (especially pyridine) is only partially deter-
harmfull than selenium.
mined. This International Standard is applicable to all
types of soils.
4 Reagents
All reagents shall be of recognized analytical grade.
Use water of grade 2 complying with ISO 3696.
4.1 Salicylic acid/sulfuric acid.
2 Normative references
Dissolve 25 g of salicylic acid in 1 litre of concentrated
sulfuric acid Q = 1,84 g/cm3).
The following Standards contain provisions which, (
through reference in this text, constitute provisions
4.2 Potassium sulfate catalyst mixture.
of this International Standard. At the time of publi-
cation, the editions indicated were valid. All Standards
Grind and thoroughly mix 200 g of potassium sulfate,
are subject to revision, and Parties to agreements
6 g of copper(ll) sulfate pentahydrate and 6 g of
based on this International Standard are encouraged
titanium dioxide, with the crystal structure of anatase.
to investigate the possibility of applying the most re-
cent editions of the Standards indicated below.
4.3 Sodium thiosulfate pentahydrate.
Members of IEC and ISO maintain registers of cur-
rently valid International Standards.
Crush the crystals to form a powder that Passes
through a sieve with an aperture of 0,25 mm.
ISO 3696: 1987, Water for analytical laboratory use -
Specifica tion and tes t me thods.
4.4 Sodium hydroxide, c(NaOH) = 10 mol/l.
ISO 5725:1986, Precision of test methods - Deter-
mina tion of repeatability and reproducibility for a
4.5 Boric acid Solution, p(H,BO,) = 20 g/l.
Standard test method by inter-laboratory tests.
4.6 Mixed indicator.
ISO 11464: 1994, Soil quality - Pretreatmen t of sam-
ples for physico-Chemical analyses.
Dissolve 0,l g of bromocresol green and 0,02 g of
methyl red in 100 ml of ethanol.
ISO 11465: 1993, Soil quality - Determination of dry
matter and water content on a mass basis -
4.7 Sulfuric acid, c(H+) = 0,Ol mol/l.
Gravimetric method.
7

---------------------- Page: 3 ----------------------
0 ISO
ISO 11261:1995(E)
under the condenser of the distillation apparatus in
5 Apparatus
such a way that the end of the condenser dips into
the Solution. Add 20 ml of sodium hydroxide (4.4) to
Usual laboratory equipment and
the funnel of the apparatus and run the alkali slowly
into the distillation chamber. Distil about 40 ml of
5.1 Digestion flasks or tubes, of nominal volume
condensate (the amount for quantitative results de-
50 ml, suitable for the digestion stand.
pends on the dimensions of the apparatus), rinse the
end of the condenser, add a few drops of indicator
5.2 Digestion stand.
(4.6) to the distillate and titrate with sulfuric acid (4.7)
to a violet endpoint.
NOTE 2 Glass tubes placed in holes drilled into an alu-
minium block are also suitable.
NOTE 5 A potentiometric titratio
...

SLOVENSKI STANDARD
SIST ISO 11261:1996
01-oktober-1996
Kakovost tal - Ugotavljanje skupnega dušika - Modificirana Kjeldahlova metoda
Soil quality -- Determination of total nitrogen -- Modified Kjeldahl method
Qualité du sol -- Dosage de l'azote total -- Méthode de Kjeldahl modifiée
Ta slovenski standard je istoveten z: ISO 11261:1995
ICS:
13.080.10 .HPLMVNH]QDþLOQRVWLWDO Chemical characteristics of
soils
SIST ISO 11261:1996 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST ISO 11261:1996

---------------------- Page: 2 ----------------------

SIST ISO 11261:1996
INTERNATIONAL
ISO
STANDARD 11261
First edition
1995-03-01
Soil quality - Determination of total
nitrogen - Modified Kjeldahl method
Quake du so/
- Dosage de I ’azo te total - Methode de Kjeldahl modifiee
Reference number
ISO 7 1261: 1995(E)

---------------------- Page: 3 ----------------------

SIST ISO 11261:1996
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation sf national Standards bodies (ISO member bodies). The work
of preparing International Standards is normally carried out through ISO
technical committees. Esch member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard ISO 11261 was prepared by Technical Committee
lSO/TC 190, Seil quality, Subcommittee SC 3, Chemical methods and seil
charac teris tics.
Annex A of this International Standard is for information only.
0 ISO 1995
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronrc or mechanical, rncluding photocopyrng and
microfilm, without Permission in writing from the publisher.
International Organization for Standardization
Case Postale 56 l CH-l 211 Geneve 20 l Switzerland
Printed in Switzerland
ii

---------------------- Page: 4 ----------------------

SIST ISO 11261:1996
ISO 11261:1995(E)
INTERNATIONAL STANDARD 0 ISO
Soil quality - Determination of total nitrogen -
Modified Kjeldahl method
3 Principle
1 Scope
The method is based on the Kjeldahl-digestion, but
This International Standard specifies a method for the
titanium dioxide (TiO,) is used as the catalyst instead
determination of the total nitrogen (ammonium-N,
of selenium.
nitrate-N, nitrite-N and organic N) content of a soil.
Nitrogen in N-N-linkages, N-0-linkages and some het-
NOTE 1 Titanium dioxide is ecotoxicologically less
erocyclics (especially pyridine) is only partially deter-
harmfull than selenium.
mined. This International Standard is applicable to all
types of soils.
4 Reagents
All reagents shall be of recognized analytical grade.
Use water of grade 2 complying with ISO 3696.
4.1 Salicylic acid/sulfuric acid.
2 Normative references
Dissolve 25 g of salicylic acid in 1 litre of concentrated
sulfuric acid Q = 1,84 g/cm3).
The following Standards contain provisions which, (
through reference in this text, constitute provisions
4.2 Potassium sulfate catalyst mixture.
of this International Standard. At the time of publi-
cation, the editions indicated were valid. All Standards
Grind and thoroughly mix 200 g of potassium sulfate,
are subject to revision, and Parties to agreements
6 g of copper(ll) sulfate pentahydrate and 6 g of
based on this International Standard are encouraged
titanium dioxide, with the crystal structure of anatase.
to investigate the possibility of applying the most re-
cent editions of the Standards indicated below.
4.3 Sodium thiosulfate pentahydrate.
Members of IEC and ISO maintain registers of cur-
rently valid International Standards.
Crush the crystals to form a powder that Passes
through a sieve with an aperture of 0,25 mm.
ISO 3696: 1987, Water for analytical laboratory use -
Specifica tion and tes t me thods.
4.4 Sodium hydroxide, c(NaOH) = 10 mol/l.
ISO 5725:1986, Precision of test methods - Deter-
mina tion of repeatability and reproducibility for a
4.5 Boric acid Solution, p(H,BO,) = 20 g/l.
Standard test method by inter-laboratory tests.
4.6 Mixed indicator.
ISO 11464: 1994, Soil quality - Pretreatmen t of sam-
ples for physico-Chemical analyses.
Dissolve 0,l g of bromocresol green and 0,02 g of
methyl red in 100 ml of ethanol.
ISO 11465: 1993, Soil quality - Determination of dry
matter and water content on a mass basis -
4.7 Sulfuric acid, c(H+) = 0,Ol mol/l.
Gravimetric method.
7

---------------------- Page: 5 ----------------------

SIST ISO 11261:1996
0 ISO
ISO 11261:1995(E)
under the condenser of the distillation apparatus in
5 Apparatus
such a way that the end of the condenser dips into
the Solution. Add 20 ml of sodium hydroxide (4.4) to
Usual laboratory equipment and
the funnel of the apparatus and run the alkali slowly
in
...

NORME
Iso
INTERNATIONALE
11261
Première édition
1995-03-01
Qualité du sol - Dosage de l’azote
total - Méthode de Kjeldahl modifiée
Soi1 quality - Determination of total nitrogen - Modified Kjeldahl
me thod
Numéro de référence
ISO 7 1261:1995(F)

---------------------- Page: 1 ----------------------
ISO 11261:1995(F)
Avant-propos
L’ISO (Organisation internationale de normalisation) est une fédération
mondiale d’organismes nationaux de normalisation (comités membres de
I’ISO). L’élaboration des Normes internationales est en général confiée aux
comités techniques de I’ISO. Chaque comité membre intéressé par une
étude a le droit de faire partie du comité technique créé à cet effet. Les
organisations internationales, gouvernementales et non gouvernemen-
tales, en liaison avec I’ISO participent également aux travaux. L’ISO colla-
bore étroitement avec la Commission électrotechnique internationale (CEI)
en ce qui concerne la normalisation électrotechnique.
Les projets de Normes internationales adoptés par les comités techniques
sont soumis aux comités membres pour vote. Leur publication comme
Normes internationales requiert l’approbation de 75 % au moins des co-
mités membres votants.
La Norme internationale ISO 11261 a été élaborée par le comité technique
lSO/TC 190, Qualité du sol, sous-comité SC 3, Méthodes chimiques et
caractéristiques du sol.
L’annexe A de la présente Norme internationale est donnée uniquement
à titre d’information.
0 ISO 1995
Droits de reproduction réservés. Sauf prescription différente, aucune partie de cette publi-
cation ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun pro-
cédé, électronique ou mécanique, y compris la photocopie et les microfilms, sans l’accord
écrit de l’éditeur.
Organisation internation ale de normali sation
Case Postale 56 l CH-l 211 Genève 2 0 l Suisse
Imprimé en Suisse
ii

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ISO 11261:1995(F)
NORME INTERNATIONALE 0 Ko
- Méthode
Qualité du sol - Dosage de l’azote total
de Kjeldahl modifiée
*
1 Domaine d’application 3 Principe
La présente méthode est fondée sur la minéralisation
La présente Norme internationale prescrit une mé-
de type Kjeldahl, mais le catalyseur utilisé est le
thode de dosage de la teneur totale en azote dans le
dioxyde de titane (TiO,), au lieu du sélénium.
sol (sous sa forme ammonium, nitrate, nitrite et or-
ganique). L’azote dans les liaisons N-N et N-O, et dans
NOTE 1 Le dioxyde de titane est moins dangereux pour
certains hétérocycles (telle que la pyridine) n’est que
l’environnement que le sélénium.
partiellement dosé. La présente Norme internationale
est applicable à tous les types de sol.
4 Réactifs
Tous les réactifs doivent être d’une pureté analytique
reconnue. Utiliser une eau de qualité 2, conformé-
2 Références normatives
ment à I’ISO 3696.
Les normes suivantes contiennent des dispositions
4.1 Acide salicylique/acide sulfurique.
qui, par suite de la référence qui en est faite, consti-
tuent des dispositions valables pour la présente
Dissoudre 25 g d’acide salicylique dans 1 litre d’acide
Norme internationale. Au moment de la publication,
sulfurique concentré Q = 1,84 g/cm3).
(
les éditions indiquées étaient en vigueur. Toute
norme est sujette à révision et les parties prenantes
catalytique au sulfate de
4.2 Mélange
des accords fondés sur la présente Norme internatio-
potassium.
nale sont invitées à rechercher la possibilité d’appli-
quer les éditions les plus récentes des normes
Broyer et mélanger jusqu’à homogénéisation com-
indiquées ci-après. Les membres de la CEI et de I’ISO
plète 200 g de sulfate de potassium, 6 g de sulfate
possèdent le registre des Normes internationales en
de cuivre pentahydraté et 6 g de dioxyde de titane
vigueur à un moment donné.
ayant la structure cristalline de I’anatase.
ISO 3696: 1987, Eau pour laboratoire à usage analyti-
4.3 Thiosulfate de sodium pentahydraté.
- Spécification et méthodes d’essai.
9ue
Réduire les cristaux en poudre afin qu’ils passent à
ISO 5725: 1986, Fidélité des méthodes d’essai -
travers un tamis de 0,25 mm d’ouverture de maille.
Détermination de la répétabilité et de la reproductibi-
lité d’une méthode d’essai normalisée par essais
4.4 Hydroxyde de sodium, c(NaOH) = 10 mol/l.
in terlabora toires.
4.5 Solution d’acide borique, p(H,BO,) = 20 g/l.
ISO 11464: 1994, Qualité du sol - Prétraitement des
échantillons pour analyses physico-chimiques.
4.6 Indicateur coloré.
ISO 11465: 1993, Qualité du sol - Détermination de
la teneur pondérale en matière sèche et en eau - Dissoudre 0,l g de vert de bromocrésol et 0,02 g de
rouge de méthyle dans 100 ml d’éthanol.
Méthode gravimé trique.

---------------------- Page: 3 ----------------------
0 ISO
ISO 11261:1995(F)
Lorsque le procédé de minéralisation est terminé,
4.7 Acide sulfurique, C(H+) = 0,Ol mol/l.
laisser refroidir le matras, et ajouter lentement, sous
agitation, approximativement 20 ml d’eau. Agiter le
5 Appareil
matras afin d’amener tout produit insoluble en sus-
pension et transférer son contenu dans l’appareil de
Matériel courar t de laboratoire, et
distillation (5.3). Rincer trois fois à l’eau pour achever
le transfert. Ajouter 5 ml d’acide borique (4.5) dans
1 tubes de minéralisation, de 50 ml,
5.1 Matras o
une fiole conique de 100 ml et placer celle-ci en sortie
adaptés au dispositif de minéralisation.
du condenseur, de manière à ce que son extrémité
plonge dans la solution. Ajouter 20 ml d’hydroxyde de
sodium (4.4) dans l’entonnoir de l’appareillage et lais-
5.2 Dispositif de minéralisation.
ser doucement couler la base dans le
...