Soil quality - Guidance on laboratory testing for biodegradation of organic chemicals in soil under aerobic conditions (ISO 11266:1994)

This International Standard provides guidance on the selection and conduct of appropriate test methods for
the determination of biodegradation of organic chemicals in aerobic soils. lt does not describe any specific test method.

Bodenbeschaffenheit - Anleitung für Laboratoriumsuntersuchungen zur biologischen Abbaubarkeit von organischen Chemikalien im Boden unter aeroben Bedingungen (ISO 11266:1994)

Diese Internationale Norm enthält eine Anleitung für die Auswahl und Durchführung geeigneter Prüf-verfahren zur Bestimmung der biologischen Abbaubarkeit von organischen Chemikalien in Böden unter aeroben Bedingungen. Sie beschreibt kein spezifisches Prüfverfahren.

Qualité du sol - Lignes directrices relatives aux essais en laboratoire pour la biodégradation de produits chimiques organiques dans le sol sous conditions aérobies (ISO 11266:1994)

Kakovost tal - Navodilo za laboratorijsko preskušanje biološke razgradljivosti organskih spojin v tleh pri aerobnih pogojih (ISO 11266:1994)

General Information

Status
Published
Public Enquiry End Date
02-Feb-2020
Publication Date
23-Sep-2020
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
19-Aug-2020
Due Date
24-Oct-2020
Completion Date
24-Sep-2020

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SLOVENSKI STANDARD
SIST EN ISO 11266:2020
01-november-2020
Kakovost tal - Navodilo za laboratorijsko preskušanje biološke razgradljivosti
organskih spojin v tleh pri aerobnih pogojih (ISO 11266:1994)

Soil quality - Guidance on laboratory testing for biodegradation of organic chemicals in

soil under aerobic conditions (ISO 11266:1994)
Bodenbeschaffenheit - Anleitung für Laboratoriumsuntersuchungen zur biologischen
Abbaubarkeit von organischen Chemikalien im Boden unter aeroben Bedingungen (ISO
11266:1994)
Qualité du sol - Lignes directrices relatives aux essais en laboratoire pour la

biodégradation de produits chimiques organiques dans le sol sous conditions aérobies

(ISO 11266:1994)
Ta slovenski standard je istoveten z: EN ISO 11266:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 11266:2020 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 11266:2020
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SIST EN ISO 11266:2020
EN ISO 11266
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Guidance on laboratory testing for
biodegradation of organic chemicals in soil under aerobic
conditions (ISO 11266:1994)

Qualité du sol - Lignes directrices relatives aux essais Bodenbeschaffenheit - Anleitung für

en laboratoire pour la biodégradation de produits Laboratoriumsuntersuchungen zur biologischen

chimiques organiques dans le sol sous conditions Abbaubarkeit von organischen Chemikalien im Boden

aérobies (ISO 11266:1994) unter aeroben Bedingungen (ISO 11266:1994)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11266:2020 E

worldwide for CEN national Members.
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SIST EN ISO 11266:2020
EN ISO 11266:2020 (E)
Contents Page

European foreword ....................................................................................................................................................... 3

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SIST EN ISO 11266:2020
EN ISO 11266:2020 (E)
European foreword

The text of ISO 11266:1994 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of the

International Organization for Standardization (ISO) and has been taken over as EN ISO 11266:2020 by

Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of

which is held by NEN.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by November 2020, and conflicting national standards

shall be withdrawn at the latest by November 2020.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,

Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,

Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of

North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the

United Kingdom.
Endorsement notice

The text of ISO 11266:1994 has been approved by CEN as EN ISO 11266:2020 without any modification.

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SIST EN ISO 11266:2020
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SIST EN ISO 11266:2020
ISO
INTERNATIONAL
11266
STANDARD
First edition
1994-09-15
Guidance on laboratory
Soil quality -
testing for biodegradation of organic
chemicals in soil under aerobic conditions
Quakt6 du so/ - Guide relatif aux essais en laboratoire pour Ia
biodegradation de produits chimiques organiques dans Ie sol sous
conditions aerobies
Reference number
ISO 11266:1994(E)
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SIST EN ISO 11266:2020
ISO 11266:1994(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation of national Standards bodies (ISO member bodies). The work of
preparing International Standards is normally carried out through ISO
technical committees. Esch member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard ISO 11266 was prepared by Technical Committee
lSO/TC 190, Seil quality, Subcommittee SC 4, Biological methods.
Annexe A of this International Standard is for information only.
0 ISO 1994

All rights reserved. Unless otherwise specified, no part of this publication may be reproduced

or utilized in any form or by any means, electronie or mechanical, including photocopying and

microfilm, without Permission in writing from the publisher.
International Organization for Standardization
Case postale 56 l CH-l 211 Geneve 20 l Switzerland
Printed in Switzerland
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SIST EN ISO 11266:2020
0 ISO ISO 11266:1994(E)
Introduction
Organic chemicals may be introduced into the soil both intentionally and
accidentally, after which they may, or may not, degrade biologically. For
chemicals which do degrade, the rate of degradation tan vary con-
siderably, depending not only on the molecular structure of the Chemical,
but also on soil conditions such as temperature, water and Oxygen
availability which influence microbial activity. The activity of micro-
organisms often plays a major role in degradative processes.
lt is necessary to have laboratory tests available to estimate the rate and
extent of biodegradation and thereby the persistente of organic chemicals
in soil. Numerous laboratory methods are available for the estimation of
aerobic biodegradation, but these differ considerably according to the
specific circumstances, for example, soil type, temperature and incubation
times.
This International Standard provides general guidelines for the selection
and conduct of tests for determining the biodegradation of organic
chemicals in aerobic soils.
of writing, fficient agreement
At the time there is insu on methodology for
biod egradability in anaerobic soils for guidelines to be prepared.
testing
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SIST EN ISO 11266:2020
Jhis page intentionaliy left blank
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SIST EN ISO 11266:2020
ISO 11266:1994(E)
INTERNATIONAL STANDARD o ISO
Soil quality - Guidance on laboratory testing for
biodegradation of organic chemicals in soil under aerobic
conditions
ISO 11261: -l), Soil quality - Determination of total
1 Scope
nitrogen - Kjeldahl method using titanium dioxide as
ca talys t.
This International Standard provides guidance on the
selection and conduct of appropriate test methods for
ISO 11274: -3) Soil quality - Determination of the
the determination of biodegradation of organic chemi-
wa ter re ten tion characteris tic - Labora tory me thods.
cals in aerobic soils. lt does not describe any specific
ISO 11277:- ‘1, Soil quality - Determination of
test method.
particle size dis tribu tion.
ISO 11461: -3 Soil quality - Determination o f soil
water content calculated on a volume basis -
2 Normative references
Gravime tric me thod.
The following Standards contain provisions which,
through reference in this text, constitute provisions of
3 Definitions
this International Standard. At the time of publication,
the editions indicated were valid. All Standards are
For the purposes of this International Standard, the
subject to revision, and Parties to agreements based
following definitions apply.
on this International Standard are encouraged to
investigate the possibility of applying the most recent
3.1 biodegradation: The molecular degradation of
editions of the Standards indicated below. Members
an organic substance resulting from the complex
of IEC and ISO maintain registers of currently valid
actions of living organisms.
International Standards.
3.2 primary biodegradation: The degradation of a
ISO 9408: 1991, VVater quality - Evaluation in an
substance to an extent sufficient to remove some
aqueous medium of the “ultimate ” aerobic biodegra-
characteristic property of the parent molecule. In
dability of organic compounds - Method by
practice, this will be determined by analysis as a loss
determining the Oxygen demand in a closed
of parent compound or some specific function of the
respirome ter.
parent compound.
ISO 10381-6: 1993, Soil quality - Sampling - Guid-
3.3. ultimate biodegradation: The breakdown of
ante on the collection, handling and. storage of soil for
an organic compound to carbon dioxide, water, the
the assessment of aerobic microbial processes in the
oxides or mineral salts of any other elements present,
labora tory.
and products associated with the normal metabolic
processes of microorganisms.
ISO 10390: 1994, Seil quality - Determination of pH.
3.4 persistente: The residente time of a Chemical
ISO 10694:- ‘1, Soil quality - Determination of
species in a specifically defined compartment of the
organic and total carbon after dry combustion
environment.
(“Element analysis “).

ISO 11260: 1994, Soil quality - Determination of 3.5 the disappearance time DT-50: The time taken

cation exchange capacity and base Saturation - for the concentration of a given compound to de-

Method using barrum chloride Solution. crease by 50 % of its original value.
1) To be published.
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SIST EN ISO 11266:2020
ISO 11266:1994(E) 0 ISO
2) field water content in accordance with
3.6 the disappearance time DT-90: The time taken
for the concentration of a given compound to de- ISO 11461;
crease by 90 % of its original value.
3) total water holding capacity and/or water
retention characteristic in accordance with
3.7 bound residues; non-extractable residues:
ISO 11274.
Chemical species in plants and soils, originating from,
for example, organic molecules that are not extracted
b) Chemical properties:
by methods which do not significantly Change the

Chemical nature of these residues. These non- 1) pH of the soil in accordance with ISO 10390,

extractable residues are considered to exclude or the pH in KCI Solution or CaC12 Solution;

fragments recycled through metabolic pathways
2) organic matter content in accordance with
leading to natura1 products. (For examples and further
ISO 10694;
information, see [3] in annex A.)
...

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