SIST EN ISO 23753-2:2019/A1:2021

Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) - Amendment 1 (ISO 23753-2:2019/Amd 1:2020)

Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 2: Verfahren mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2019/Amd 1:2020)

No scope available

Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 2: Méthode au chlorure de iodotétrazolium (INT) - Amendement 1 (ISO 23753-2:2019/Amd 1:2020)

Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 2. del: Metoda z jodotetrazolijevim kloridom (INT) - Dopolnilo A1 (ISO 23753-2:2019/Amd 1:2020)

General Information

• Status: Published
• Public Enquiry End Date: 03-May-2020
• Publication Date: 06-Dec-2020
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: KAT - Soil quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 23-Nov-2020
• Due Date: 28-Jan-2021
• Completion Date: 07-Dec-2020
• Ref Project: EN ISO 23753-2:2019/A1:2020 - Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) - Amendment 1 (ISO 23753-2:2019/Amd 1:2020)

Relations

• Amends: SIST EN ISO 23753-2:2019 - Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) (ISO 23753-2:2019)
• Effective Date: 01-Jan-2021

Overview

SIST EN ISO 23753-2:2019/A1:2021 is an internationally recognized European standard that specifies a method for determining dehydrogenase activity in soils. This technique utilizes iodotetrazolium chloride (INT) to accurately assess soil biological quality through enzymatic activity measurement. Developed by ISO Technical Committee 190 and endorsed by the European Committee for Standardization (CEN), this amendment updates the original 2019 version, providing refined procedural guidelines for enhanced reliability.

The standard is essential for soil scientists, environmental researchers, and agricultural professionals who require precise soil quality assessment tools. Dehydrogenases are important soil enzymes involved in microbial oxidative activities, serving as indicators of overall soil health and microbial function.

Key Topics

• Dehydrogenase Activity: Measurement of this enzyme's activity reflects microbial metabolic status and soil biological activity.
• Use of Iodotetrazolium Chloride (INT): This chemical acts as a substrate to produce a quantifiable colored compound, iodonitrotetrazolium formazan (INTF), allowing spectrophotometric analysis.
• Standard Curve and Calculation: The amended method emphasizes constructing a linear standard curve correlating INTF concentration (nmol/ml) to its absorbance, improving accuracy.
• Formula for Enzymatic Activity:
  The dehydrogenases activity (A) is calculated considering:
  • INTF concentrations in sample and control tubes
  • Reaction volume and time
  • Soil mass and dry matter content (%DM)
    This quantification is expressed in milliunits per gram of dry soil sample (mU/g), facilitating comparability between samples.
• Compliance and Standardization: This amendment requires member states of CEN to adopt it by May 2021, ensuring harmonized soil quality evaluation across Europe.

Applications

• Soil Quality Monitoring: Essential for tracking soil biological health in agriculture, forestry, and natural ecosystems.
• Environmental Impact Assessments: Evaluates effects of pollutants or land use changes on soil microbial activity.
• Land Remediation Projects: Assists in monitoring recovery and restoration of microbial function in contaminated or disturbed soils.
• Research and Development: Provides a standardized laboratory method for scientific studies regarding soil microbiology and enzymatic processes.
• Agricultural Management: Supports decision-making to optimize soil fertility and microbial balance for crop production.

Related Standards

• ISO 23753 Series: This document is part 2 of the overall ISO 23753 series focused on soil dehydrogenase activity determination methods.
• ISO 11465: Specifies soil dry matter content measurement, critical for calculating enzyme activity accurately.
• ISO/TC 190 Soil Quality Standards: Other related standards cover physical, chemical, and biological soil properties to complement enzyme activity evaluations.
• CEN/TC 444 Environmental Characterization: Provides standards for testing and characterizing solid environmental matrices, reinforcing soil quality assessments.

---

Keywords: EN ISO 23753-2, soil quality, dehydrogenase activity, iodotetrazolium chloride, INT method, soil enzyme assay, soil microbial activity, environmental soil testing, standardized soil analysis, biological soil properties, soil health indicator, CEN soil standard.

Standards Content (Sample)

SLOVENSKI STANDARD
01-januar-2021
Kakovost tal - Določevanje aktivnosti dehidrogenaze v tleh - 2. del: Metoda z
jodotetrazolijevim kloridom (INT) - Dopolnilo A1 (ISO 23753-2:2019/Amd 1:2020)
Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using
iodotetrazolium chloride (INT) - Amendment 1 (ISO 23753-2:2019/Amd 1:2020)
Bodenbeschaffenheit - Bestimmung der Dehydrogenaseaktivität in Böden - Teil 2:
Verfahren mit Iodotetrazoliumchlorid (INT) (ISO 23753-2:2019/Amd 1:2020)
Qualité du sol - Détermination de l'activité des déshydrogénases dans les sols - Partie 2:
Méthode au chlorure de iodotétrazolium (INT) - Amendement 1 (ISO 23753-2:2019/Amd
1:2020)
Ta slovenski standard je istoveten z: EN ISO 23753-2:2019/A1:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 23753-2:2019/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Determination of dehydrogenases activity in
soils - Part 2: Method using iodotetrazolium chloride (INT)
- Amendment 1 (ISO 23753-2:2019/Amd 1:2020)
Qualité du sol - Détermination de l'activité des Bodenbeschaffenheit - Bestimmung der
déshydrogénases dans les sols - Partie 2: Méthode au Dehydrogenaseaktivität in Böden - Teil 2: Verfahren
chlorure de iodotétrazolium (INT) - Amendement 1 mit Iodotetrazoliumchlorid (INT) (ISO 23753-
(ISO 23753-2:2019/Amd 1:2020) 2:2019/Amd 1:2020)
This amendment A1 modifies the European Standard EN ISO 23753-2:2019; it was approved by CEN on 28 October 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23753-2:2019/A1:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 23753-2:2019/A1:2020) has been prepared by Technical Committee ISO/TC
190 "Soil quality" in collaboration with Technical Committee CEN/TC 444 “Environmental
characterization of solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2021, and conflicting national standards shall be
withdrawn at the latest by May 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 23753-2:2019/Amd 1:2020 has been approved by CEN as EN ISO 23753-
2:2019/A1:2020 without any modification.

----------
...