Algae and algae products - Methods of sampling and analysis - Sample treatment

This document describes the sample preparation of dry and wet samples of micro- and macroalgae, algae-based products and intermediates. This document enables laboratories analysing algae samples to report accurate dry weight percentages and to obtain representative samples possible for further examination.

Algen und Algenprodukte - Methoden zur Probeentnahme und Analyse - Probebehandlung

Dieses Dokument beschreibt die Probenvorbereitung von trockenen und feuchten Proben von Mikro  und Makroalgen, algenbasierten Produkten und Zwischenprodukten. Dieses Dokument ermöglicht es Laboratorien, die Algenproben analysieren, genaue Angaben des Prozentsatzes bezogen auf die Trockenmasse zu machen und repräsentative Proben für weitere Untersuchungen zu erhalten.

Algues et produits à base d’algues - Méthodes d’échantillonnage et d’analyse - Traitement des échantillons

Le présent document décrit la préparation des échantillons secs et humides de micro- et de macro algues, ainsi que de produits et d’intermédiaires à base d’algues. Le présent document permet aux laboratoires qui analysent des échantillons d’algues de consigner dans leurs rapports des pourcentages de poids sec fiables et d’obtenir des échantillons représentatifs en vue de leur examen ultérieur.

Alge in izdelki iz alg - Metode vzorčenja in analize - Obdelava vzorca

General Information

Status
Not Published
Public Enquiry End Date
31-Dec-2020
Technical Committee
Current Stage
5020 - Formal vote (FV) (Adopted Project)
Start Date
05-Aug-2021
Due Date
23-Sep-2021
Completion Date
05-Aug-2021

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SLOVENSKI STANDARD
oSIST prEN 17605:2020
01-december-2020

Alge, izdelki iz alg in vmesni proizvodi - Metode vzorčenja in analize - Obdelava

vzorca

Algae, algae products and intermediates - Methods of sampling and analysis - Sample

treatment
Algen, Algenprodukte und Zwischenprodukte - Methoden zur Probeentnahme und
Analyse - Probebehandlung
Algues et produits à base d’algues - Méthodes d’échantillonnage et d’analyse -
Traitement des échantillons
Ta slovenski standard je istoveten z: prEN 17605
ICS:
13.020.55 Biološki izdelki Biobased products
oSIST prEN 17605:2020 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 17605:2020
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oSIST prEN 17605:2020
DRAFT
EUROPEAN STANDARD
prEN 17605
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2020
ICS 13.020.55
English Version
Algae, algae products and intermediates - Methods of
sampling and analysis - Sample treatment

Algues et produits à base d'algues - Méthodes Algen, Algenprodukte und Zwischenprodukte -

d'échantillonnage et d'analyse - Traitement des Methoden zur Probeentnahme und Analyse -

échantillons Probebehandlung

This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee

CEN/TC 454.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations

which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other

language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC

Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are

aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without

notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17605:2020 E

worldwide for CEN national Members.
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Contents Page

European foreword ............................................................................................................................................ 3

Introduction .......................................................................................................................................................... 4

1 Scope .......................................................................................................................................................... 5

2 Normative references .......................................................................................................................... 5

3 Terms and definitions ......................................................................................................................... 5

4 Principle ................................................................................................................................................... 8

5 Apparatus ................................................................................................................................................ 8

6 Reagents and materials ...................................................................................................................... 9

7 Procedures .............................................................................................................................................. 9

8 Calculations ........................................................................................................................................... 14

9 Quality control ..................................................................................................................................... 15

10 Report ..................................................................................................................................................... 15

Annex A (informative) Example of a sample registration form ........................................................ 16

Bibliography ....................................................................................................................................................... 18

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European foreword

This document (prEN 17605:2020) has been prepared by Technical Committee CEN/TC 454 “Algae and

algae products”, the secretariat of which is held by NEN.
This document is currently submitted to the CEN Enquiry.

This document has been prepared under a mandate given to CEN by the European Commission and the

European Free Trade Association.
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Introduction

This document has been prepared by the experts of CEN/TC 454 'Algae and algae products'.

The European Committee for Standardization (CEN) was requested by the European Commission (EC) to

draft European standards or European standardization deliverables to support the implementation of

Article 3 of Directive 2009/28/EC for algae and algae-based products or intermediates.

This request, presented as Mandate M/547 , also contributes to the Communication on “Innovating for

Sustainable Growth: A Bio economy for Europe”.

The former working group CEN Technical Board Working Group 218 “Algae”, was created in 2016 to

develop a work programme as part of this Mandate. The technical committee CEN/TC 454 'Algae and

algae products' was established to carry out the work programme that will prepare a series of standards.

The interest in algae and algae-based products or intermediates has increased significantly in Europe as

a valuable source including but not limited to carbohydrates, proteins, lipids, and several pigments. These

materials are suitable for use in a wide range of applications from food and feed purposes to other sectors,

such as textile, cosmetics, biopolymers, biofuel and fertilizer/biostimulants. Standardization was

identified as having an important role in order to promote the use of algae and algae products.

The work of CEN/TC 454 should improve the reliability of the supply chain, thereby improving the

confidence of industry and consumers in algae, which include macroalgae, microalgae, cyanobacteria,

labyrinthulomycetes, algae-based products or intermediates and will promote and support

commercialisation of the European algae industry.

This document has been developed with the aim to enable laboratories to conduct a standardized

procedure for sample preparation to be used for analysing samples of micro- and macroalgae, algae-

based products and intermediates.

Available at http://ec.europa.eu/growth/tools-databases/mandates/index.cfm?fuseaction=refSearch.search#

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1 Scope

This document describes the sample preparation of dry and wet samples of micro- and macroalgae, algae-

based products and intermediates. This document enables laboratories analysing algae samples to report

accurate dry weight percentages and to obtain representative samples possible for further examination.

2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 24333:2009, Cereals and cereal products — Sampling

ISO 5667-1:2006, Water quality — Sampling — Part 1: Guidance on the design of sampling programmes

and sampling techniques
3 Terms and definitions

For the purposes of this document, the terms and definitions given in EN 17399:2020 and the following

apply.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
lot

quantity of material that is assumed to be of the same production process and represented by specified

sampling rules
[SOURCE: ISO 6498:2012, definition 2.2.1]
3.2
laboratory sample

sample as prepared (from the lot) for sending to the laboratory and intended for inspection or testing

[SOURCE: ISO 6498:2012, definition 2.1.2]
3.3
test sample

subsample or sample prepared from the laboratory sample and from which test portions will be taken

[SOURCE: ISO 6498:2012, definition 2.1.3]
3.4
test portion

quantity of material drawn from the test sample or from the laboratory sample if both are the same

[SOURCE: ISO 6498:2012, definition 2.1.4]
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3.5
reserve sample

material left over from the laboratory sample when divided or subsampled test samples have been taken

and on which no further particle size reduction is done
[SOURCE: ISO 6498:2012, definition 2.1.5]
3.6
sample

laboratory sample, a test sample, a test portion or a reserve sample as described in (3.2), (3.3), (3.4), (3.5)

3.7
dry sample
sample with a moisture content ≤15 % of the total mass
3.8
wet sample
sample with a moisture content > 15 % of the total mass
3.9
homogeneity

degree to which a property or a constituent is uniformly distributed throughout a quantity of material

[SOURCE: ISO 6498:2012, definition 2.4.1]

Note 1 to entry: Homogeneity may be considered to having been achieved in a practical sense when the sampling

error of the processed portion is negligible compared to the total error of the measurement system. Since

homogeneity depends on the size of the units under consideration, a mixture of two materials may be

inhomogeneous at the molecular or atomic level, but sufficiently homogeneous at the particulate level. However,

uniform visual appearance does not ensure compositional homogeneity.
3.10
homogenization

procedure to assure the homogeneity of the sample, including coarse grinding, fine grinding, mixing,

agitating, blending and pulverization
3.11
partial drying

drying procedure for wet samples, in which the sample is carefully dried to allow subsequent sample

preparation procedures to be applied

Note 1 to entry: Partial drying can be performed by oven drying or freeze drying and is presented in % moisture

content reduction.
3.12
coarse grinding

first grinding step of the whole sample when the laboratory sample contains large lumps or when its

particle size is above about 6 mm before mass reduction
[SOURCE: ISO 6498:2012, definition 2.4.3]

Note 1 to entry: Coarse grinding is a special kind of particle size reduction that ensures homogeneity of the

laboratory sample for subsampling purposes.
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3.13
fine grinding

grinding procedure for reducing the particle size to the requested 200-500 µm, achieved by chopping,

crushing, cutting, macerating, milling (grinding), pressing to obtain a homogeneous test sample for

further analysis
3.14
subsampling (splitting)

dividing the pre-treated and homogenized laboratory sample into the test sample and the reserve sample

Note 1 to entry: After subsampling (splitting), all subsamples should have the same properties as the original

laboratory sample.

Note 2 to entry: Definition is similar to definition of mass reduction (ISO 6498:2012).

3.15
true value

value which would be obtained under ideal measuring conditions where no errors occur

3.16
moisture content
loss on drying

mass/mass volatile fraction of the sample lost after drying under test conditions

3.17
dry weight (d.w.)
mass corrected for 100 % dry matter (i.e. a moisture content of 0 %)
3.18
parameter

analyte or constituent or microorganism for which the sample is to be analysed by microscopic,

microbiological, biological or chemical procedures
[SOURCE: ISO 6498:2012, definition 2.2.1]
3.19
stable parameter

analyte or constituent or microorganism which does not degrade during sample preparation on common

handling or storage at room temperatures of 20 °C to 25 °C
[SOURCE: ISO 6498:2012, definition 2.2.1.1]
3.20
unstable parameter

analyte or constituent or microorganism which degrades during sample preparation on common

handling or storage at room temperatures of 20 °C to 25 °C because they are volatile, degradable, or

sensitive to temperature, light, enzymatic degradation or chemical oxidation
[SOURCE: ISO 6498:2012, definition 2.2.1.2]
The relations between the different definitions are given in Figure 1
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Figure 1 — Illustration of relation between the different samples types and sample preparation

procedures
4 Principle

Proper sample preparation is one of the crucial steps in obtaining the best possible result that most likely

corresponds to the “true value”. All sample treatment steps depend on the different properties of the

sample material and on the parameters to be analysed. Algae, algae products and intermediates are

biological material, and not all parameters are stable. Therefore, special instructions concerning sample

preparation for the different analysis methods are described in Clause 7.
5 Apparatus

The apparatus used for sample treatment consist of usual laboratory equipment and in particular the

following. All equipment used should be appropriate to reduce the risk of contamination and oxidation

during sample preparation. More information regarding the criteria for design and proper use is given in

ISO 6498:2012.
5.1 General
The equipment required includes:
5.1.1 Analytical balance with an accuracy of 0,01 mg
5.1.2 Mechanical mill or mechanical microgrinder able to grind 90 % below 500 µm
5.2 Sample storage

In order to provide adequate storage the following facilities should where necessary be provided:

5.2.1 Desiccator containing dry desiccant
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