Water quality - Technical report for the routine sampling of benthic diatoms from rivers and lakes adapted for metabarcoding analyses

This technical report specifies a method for the field sampling of benthic diatoms which will be then analysed by subsequent metabarcoding techniques for ecological status and water quality assessments. Data produced by this method are suitable for production of taxonomical diatom lists.

Wasserbeschaffenheit - Technischer Bericht zur Routine-Probenahme von benthischen Diatomeen in Flüssen und Seen für Metabarcode-Analysen

Qualité de l'eau - Rapport technique concernant l'échantillonnage de routine de diatomées benthiques dans les rivières et les lacs adapté aux analyses reposant sur l'utilisation de métacodes à barres d'ADN

Le présent rapport technique spécifie une méthode d’échantillonnage sur le terrain de diatomées
benthiques qui seront ensuite analysées par des techniques ultérieures en metabarcoding afin d’évaluer
l’état écologique et la qualité de l’eau. Les données produites par cette méthode sont appropriées pour
produire des listes de diatomées taxinomiques.

Kakovost vode - Tehnično poročilo o rutinskem vzorčenju bentoških kremenastih alg v rekah in jezerih, prilagojeno za analize metabarkodiranja

To tehnično poročilo določa metodo za vzorčenje bentoških kremenastih alg na terenu, ki se nato analizirajo z naknadnimi tehnikami metabarkodiranja za ekološko stanje in oceno kakovosti vode. Podatki, pridobljeni s to metodo, so primerni za izdelavo taksonomskih seznamov kremenastih alg.

General Information

Status
Published
Public Enquiry End Date
01-Apr-2018
Publication Date
06-Jun-2019
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
14-Mar-2019
Due Date
19-May-2019
Completion Date
07-Jun-2019

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SLOVENSKI STANDARD
SIST-TP CEN/TR 17245:2019
01-julij-2019
Kakovost vode - Tehnično poročilo o rutinskem vzorčenju bentoških kremenastih
alg v rekah in jezerih, prilagojeno za analize metabarkodiranja

Water quality - Technical report for the routine sampling of benthic diatoms from rivers

and lakes adapted for metabarcoding analyses

Wasserbeschaffenheit - Technischer Bericht zur Routine-Probenahme von benthischen

Diatomeen in Flüssen und Seen für Metabarcode-Analysen
Qualité de l'eau - Rapport technique concernant l'échantillonnage de routine de

diatomées benthiques dans les rivières et les lacs adapté aux analyses reposant sur

l'utilisation de métacodes à barres d'ADN
Ta slovenski standard je istoveten z: CEN/TR 17245:2018
ICS:
13.060.10 Voda iz naravnih virov Water of natural resources
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST-TP CEN/TR 17245:2019 en

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TP CEN/TR 17245:2019
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SIST-TP CEN/TR 17245:2019
CEN/TR 17245
TECHNICAL REPORT
RAPPORT TECHNIQUE
September 2018
TECHNISCHER BERICHT
ICS 13.060.70
English Version
Water quality - Technical report for the routine sampling
of benthic diatoms from rivers and lakes adapted for
metabarcoding analyses

Qualité de l'eau - Rapport technique pour Wasserbeschaffenheit - Technischer Bericht zur

l'échantillonnage en routine de diatomées benthiques routinemäßigen Beprobung benthischer Diatomeen

dans les rivières et les plans d'eau adapté pour les aus Fließgewässern und Seen angepasst für

analyses en metabarcoding Metabarcodinganalysen

This Technical Report was approved by CEN on 14 May 2018. It has been drawn up by the Technical Committee CEN/TC 230.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,

Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TR 17245:2018 E

worldwide for CEN national Members.
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SIST-TP CEN/TR 17245:2019
CEN/TR 17245:2018 (E)
Contents Page

European foreword ....................................................................................................................................................... 3

Introduction .................................................................................................................................................................... 4

1 Scope .................................................................................................................................................................... 5

2 Normative references .................................................................................................................................... 5

3 Terms and definitions ................................................................................................................................... 5

4 Principle ............................................................................................................................................................. 5

4.1 General ................................................................................................................................................................ 5

4.2 Equipment for Field sampling .................................................................................................................... 5

5 Reagents ............................................................................................................................................................. 6

5.1 General ................................................................................................................................................................ 6

5.2 Preservatives .................................................................................................................................................... 6

5.2.1 General ................................................................................................................................................................ 6

5.2.2 90 % Ethanol (C2H5OH) ............................................................................................................................... 6

5.2.3 Deep-freezing ................................................................................................................................................... 6

5.2.4 Other commercial preservatives ............................................................................................................... 6

6 Procedure........................................................................................................................................................... 6

6.1 Choice of substratum ..................................................................................................................................... 6

6.2 Sample site selection ...................................................................................................................................... 6

6.3 Sampling methods .......................................................................................................................................... 6

6.3.1 Moveable natural hard surfaces ................................................................................................................ 6

6.3.2 Method for sampling vertical man-made surfaces in situ ................................................................ 6

6.3.3 Use of introduced (“artificial”) substrata ............................................................................................... 7

6.3.4 Sample collection from submerged macrophytes and macroalgae .............................................. 7

6.3.5 Sample collection from emergent macrophytes .................................................................................. 7

6.4 Sample preservation ...................................................................................................................................... 7

6.4.1 Ethanol preservation ..................................................................................................................................... 7

6.4.2 Deep-freezing preservation ........................................................................................................................ 7

6.4.3 Other commercial preservatives ............................................................................................................... 7

Bibliography .................................................................................................................................................................... 8

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SIST-TP CEN/TR 17245:2019
CEN/TR 17245:2018 (E)
European foreword

This document (CEN/TR 17245:2018) has been prepared by Technical Committee CEN/TC 230 “Water

analysis”, the secretariat of which is held by DIN.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

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SIST-TP CEN/TR 17245:2019
CEN/TR 17245:2018 (E)
Introduction

WARNING — Persons using this document should be familiar with normal laboratory practice. This

technical report does not purport to address all of the safety problems, if any, associated with its use. It

is the responsibility of the user to establish appropriate health and safety practices and to ensure

compliance with any national regulatory conditions.

Diatoms are unicellular microalgae present in all types of water bodies. They are an important

component of aquatic ecosystems and have been used widely for ecological assessment required by the

Water Framework Directive (2000/60/EC) and Urban Waste Water Treatment Directive (91/217/EEC)

in addition to other EU Directives and international agreements. The use of diatoms as indicators of

river and lake quality is based on observations that diatom taxa have distinct preferences for particular

environmental conditions such as nutrients, organic pollution and acidity. Polluted waters will tend to

support higher proportions of those taxa whose optima correspond with the levels of the pollutant in

question. Conversely, certain species are intolerant of elevated levels of one or more pollutants, whilst

others may occur in a wide range of water qualities.

Methods using diatoms to assess water quality and ecological status based on optical microscopy have

been developed in several European countries. Methods for sampling and preparation are similar [6],

[7] leading to the development of European Standards which, in turn, facilitated the harmonization of

ecological assessment approaches [2], [3]. More r
...

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