Foods of plant origin - Multimethod for the determination of pesticide residues using GC- and LC-based analysis following acetonitrile extraction/partitioning and clean-up by dispersive SPE - Modular QuEChERS-method

This European Standard stipulates a method for the analysis of pesticide residues in foods of plant origin, such as fruits (including dried fruits), vegetables, cereals and many processed products thereof by using GC, GC-MS(/MS), and/or LC-MS(/MS). The method has been collaboratively studied on a large number of commodity/pesticide combinations. Precision data are summarized in FprCEN/TR 17063. Guidelines for calibration are outlined in FprCEN/TS 17061.

Pflanzliche Lebensmittel - Multiverfahren zur Bestimmung von Pestizidrückständen mit GC und LC nach Acetonitril-Extraktion/Verteilung und Reinigung mit dispersiver SPE - Modulares QuEChERS-Verfahren

Diese Europäische Norm beschreibt ein Verfahren für die Analyse von Pestizidrückständen in pflanzlichen Lebensmitteln wie z. B. Früchten (einschließlich Trockenfrüchten), Gemüse (einschließlich getrocknetem Gemüse), Getreide und vielen verarbeiteten Erzeugnissen aus diesen Produkten unter Verwendung von GC, GC MS(/MS) und/oder LC MS(/MS). Das Verfahren wurde im Ringversuch mit einer großen Anzahl an Rohstoff /Pestizidkombinationen geprüft. Die Präzisionsdaten sind in CEN/TR 17063 zusammen¬gefasst. Leitfäden für die Kalibrierung sind in CEN/TS 17061 angegeben.

Aliments d'origine végétale - Multiméthode de détermination des résidus de pesticides par analyse CG et CL après extraction/partition avec de l'acétonitrile et purification par SPE dispersive - Méthode modulaire QuEChERS

La présente Norme européenne décrit une méthode d’analyse CG, CG-SM(/SM) et/ou CL-SM(/SM) des résidus de pesticides dans les aliments d’origine végétale, notamment les fruits (y compris les fruits secs), les légumes, les céréales et les nombreux produits transformés dérivés de ceux-ci. La méthode a fait l’objet d’un essai interlaboratoires portant sur plusieurs combinaisons denrée/pesticide. Les données de fidélité sont résumées dans le FprCEN/TR 17063. Les lignes directrices relatives à l’étalonnage sont données dans la FprCEN/TS 17061.

Hrana rastlinskega izvora - Večelementna metoda za določanje ostankov pesticidov z uporabo analize na osnovi GC in LC po delitvi in izpiranju acetonitrila z disperzivno SPE - Modularna metoda QuEChERS

Ta evropski standard določa metodo za analizo ostankov pesticidov v živilih rastlinskega izvora, kot so sadje (vključno s suhim sadjem), zelenjava, žitarice in številni predelani izdelki rastlinskega izvora z uporabo GC, GC-MS(/MS) in/ali LC-MS(/MS). Ta metoda je bila raziskana z večstranskimi študijami na veliki količini kombinacij proizvodov in pesticidov. Podatki o natančnosti so povzeti v standardu FprCEN/TR 17063. Smernice za umerjanje so opisane v standardu FprCEN/TS 17061.

General Information

Status
Published
Public Enquiry End Date
19-Mar-2017
Publication Date
06-Jun-2018
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
30-May-2018
Due Date
04-Aug-2018
Completion Date
07-Jun-2018

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SLOVENSKI STANDARD
SIST EN 15662:2018
01-julij-2018
1DGRPHãþD
SIST EN 15662:2009
+UDQDUDVWOLQVNHJDL]YRUD9HþHOHPHQWQDPHWRGD]DGRORþDQMHRVWDQNRY

SHVWLFLGRY]XSRUDERDQDOL]HQDRVQRYL*&LQ/&SRGHOLWYLLQL]SLUDQMXDFHWRQLWULOD

]GLVSHU]LYQR63(0RGXODUQDPHWRGD4X(&K(56

Foods of plant origin - Multimethod for the determination of pesticide residues using GC-

and LC-based analysis following acetonitrile extraction/partitioning and clean-up by

dispersive SPE - Modular QuEChERS-method

Pflanzliche Lebensmittel - Multiverfahren zur Bestimmung von Pestizidrückständen mit

GC und LC nach Acetonitril-Extraktion/Verteilung und Reinigung mit dispersiver SPE -

Modulares QuEChERS-Verfahren

Aliments d'origine végétale - Multiméthode de détermination des résidus de pesticides

par analyse CG et CL après extraction/partition avec de l'acétonitrile et purification par

SPE dispersive - Méthode modulaire QuEChERS
Ta slovenski standard je istoveten z: EN 15662:2018
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST EN 15662:2018 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 15662:2018
---------------------- Page: 2 ----------------------
SIST EN 15662:2018
EN 15662
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2018
EUROPÄISCHE NORM
ICS 67.050 Supersedes EN 15662:2008
English Version
Foods of plant origin - Multimethod for the determination
of pesticide residues using GC- and LC-based analysis
following acetonitrile extraction/partitioning and clean-up
by dispersive SPE - Modular QuEChERS-method

Aliments d'origine végétale - Multiméthode de Pflanzliche Lebensmittel - Multiverfahren zur

détermination des résidus de pesticides par analyse CG Bestimmung von Pestizidrückständen mit GC und LC

et CL après extraction/partition avec de l'acétonitrile nach Acetonitril-Extraktion/Verteilung und Reinigung

et purification par SPE dispersive - Méthode modulaire mit dispersiver SPE - Modulares QuEChERS-Verfahren

QuEChERS
This European Standard was approved by CEN on 27 December 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,

Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15662:2018 E

worldwide for CEN national Members.
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SIST EN 15662:2018
EN 15662:2018 (E)
Contents Page

European foreword ....................................................................................................................................................... 3

1 Scope .................................................................................................................................................................... 4

2 Normative references .................................................................................................................................... 4

3 Principle ............................................................................................................................................................. 4

4 Preparation and storage of the samples ................................................................................................. 4

5 Procedure........................................................................................................................................................... 5

6 Evaluation of results ................................................................................................................................... 15

7 Confirmatory tests ....................................................................................................................................... 22

8 Precision .......................................................................................................................................................... 22

9 Test report ...................................................................................................................................................... 22

Annex A (informative) Description of modules .............................................................................................. 23

Annex B (informative) Complementary information ................................................................................... 76

Annex C (informative) Abbreviations ................................................................................................................ 78

Bibliography ................................................................................................................................................................. 80

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SIST EN 15662:2018
EN 15662:2018 (E)
European foreword

This document (EN 15662:2018) has been prepared by Technical Committee CEN/TC 275 “Food

analysis - Horizontal methods”, the secretariat of which is held by DIN.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by November 2018, and conflicting national standards

shall be withdrawn at the latest by November 2018.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN 15662:2008.

With the revised version, some amendments and improvements have been taken into consideration,

notably:

— the more precise differentiation between feasible modes of operation (Table 1 to Table 5);

— the opportunity to report the applied modes of operation (e.g. extraction or clean-up modules) in a

simple way;

— clear indications of approved modes of operation for particular commodities (Table 6);

— the optimization of extraction efficiency by longer extraction time;

— the specification of suitable parameters for the detection with UPLC-MS/MS and GC-MS/MS;

— new approaches for the quantitation of pesticide residues including a simplified procedure for the

calculation of residue levels;

— references to the improved validation data for the method (see Table 7 and CEN/TR 17063);

— a list of abbreviations has been added in Annex C.

WARNING — The application of this standard may involve hazardous materials, operations and

equipment. This standard does not claim to address all the safety problems associated with its

use. It is the responsibility of the user of this standard to establish appropriate safety and health

practices and to determine the applicability of regulatory limitations prior to use.

According to the CEN-CENELEC Internal Regulations, the national standards organisations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,

Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,

France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,

Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,

Turkey and the United Kingdom.
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SIST EN 15662:2018
EN 15662:2018 (E)
1 Scope

This European Standard stipulates a method for the analysis of pesticide residues in foods of plant

origin, such as fruits (including dried fruits), vegetables (including dried vegetables), cereals and many

processed products thereof by using GC, GC-MS(/MS), and/or LC-MS(/MS). The method has been

collaboratively studied on a large number of commodity/pesticide combinations. Precision data are

summarized in CEN/TR 17063. Guidelines for calibration are outlined in CEN/TS 17061.

2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

CEN/TS 17061:2017, Foodstuffs - Guidelines for the calibration and quantitative determination of

pesticide residues and organic contaminants using chromatographic methods
3 Principle

The homogeneous sample is extracted with the help of acetonitrile. Samples with low water content

(<80 %) require the addition of water before the initial extraction to get a total of approximately 10 g of

water. After addition of magnesium sulfate, sodium chloride and buffering citrate salts, the mixture is

shaken intensively and centrifuged for phase separation. An aliquot of the organic phase is cleaned-up

by dispersive solid phase extraction (D-SPE) employing bulk sorbents as well as magnesium sulfate for

the removal of residual water. Following clean-up with amino-sorbents (e.g. primary secondary amine

sorbent, PSA) and if necessary graphitized carbon black (GCB) or octadecylsilane (ODS), extracts are

acidified by adding a small amount of formic acid, to improve the storage stability of certain base-

sensitive pesticides. The final extract can be directly employed for GC- and LC-based analysis. Suitable

detectors for GC analysis are mass-selective detectors (MS or MS/MS) with unit or high mass resolution

or other GC detectors, such as flame photometric detector, FPD, and electron capture detector, ECD. For

the analysis with LC hyphenations with tandem mass-spectrometry (LC-MS/MS) or high resolution

mass-spectrometry are particularly suitable. Quantification may be performed using an internal

standard, which is added to the test portion before the first extraction, but this is not mandatory. Details

for calibration, see CEN/TS 17061.
4 Preparation and storage of the samples
4.1 General

Sample processing and storage procedures should be demonstrated to have no significant effect on the

residues present in the test sample (sometimes also called “analytical sample”). Processing should also

ensure that the test sample is homogeneous enough so that portion to portion (sub-sampling)

variability is acceptable. If a single analytical portion is unlikely to be representative of the test sample,

larger or replicate portions shall be analysed, to provide a better estimate of the true value. The degree

of comminution should support a quantitative residue extraction.
4.2 Laboratory sample

A laboratory sample that is wholly or extensively spoiled or degraded should not be analysed. When

possible, prepare laboratory samples immediately after arrival and in any event, before any significant

physical or chemical changes have taken place. If a laboratory sample cannot be prepared without

delay, it should be stored under appropriate conditions to keep it fresh and to avoid deterioration.

Dried or similarly processed samples should be analysed within their stated shelf life.

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SIST EN 15662:2018
EN 15662:2018 (E)
4.3 Partly-prepared test sample

For preparation of the partly-prepared test sample take only the portion of the laboratory sample to

which the maximum residue level applies. No further plant-parts shall be removed.

The reduction of the laboratory sample shall be carried out in such a way that representative portions

are obtained (e.g. by sub-division into four and selection of opposite quarters). For samples of small

units (e.g. small fruits such as berries, legumes, cereals), the sample shall be thoroughly mixed before

weighing out the partly-prepared test sample. When the samples are made up of larger units, take

wedge-shaped sections (e.g. melons) or cross sections (e.g. cucumbers) that include the skin (outer

surface) from each unit [1].
4.4 Test sample

From each partly-prepared test sample, any parts that would cause difficulties with the homogenization

process should be removed. In the case of stone fruits, the stones shall be removed. This is the test

sample. A record of the plant-parts that have been removed shall be kept. Precautions should be taken

to avoid any losses of juice or flesh. Calculation of the residue shall be based on the mass of the original

test sample (including the stones where it is necessary).

Where the homogeneity of the test sample is not sufficient or the extraction of residues may be

significantly compromised due to large particle sizes, intensive comminution should be performed

using appropriate means. This is possible at ambient temperature, if separation of flesh and juice or

degradation of target pesticides does not occur to a significant extent. Comminution of samples in a

frozen state can significantly reduce losses of chemically labile pesticides and usually results in smaller

particle sizes and a higher degree of homogeneity. Cutting the samples coarsely (e.g. 3 cm x 3 cm) with a

knife and putting them into the freezer (e.g. −18 °C overnight) prior to comminution facilitates

processing. Processing can be also assisted and improved by cryogenic milling (using dry ice or liquid

nitrogen) by keeping the temperature below 0 °C. Especially in the case of fruits and vegetables,

cryogenic milling is much more effective at homogenizing commodities that have tough skins (e.g.

tomatoes or grapes) compared to milling at ambient temperature. Given the fact that non-systemic

pesticides often predominantly occur on the skin, cryogenic milling significantly reduces sub-sampling

variability. When processing test samples at low temperatures, condensation caused by high humidity

should be avoided. Residual carbon dioxide should be allowed to sufficiently dissipate so that its

contribution to weigh of the sample will be negligible.
4.5 Test portion

Individual test portions each sufficient for one analysis should be taken from the comminuted test

sample. These test portions should be analysed immediately. If test portions cannot be analysed

directly, the test sample or the test portions shall be frozen until required. If it is noted that

homogeneity of the test sample has been compromised during storage, the test sample shall be mixed

before taking test portions to ensure that homogeneity has been re-established.
5 Procedure

Extraction of samples is specified through modules E1 to E9. Extraction is usually followed by a clean-

up of the obtained raw extracts using the modules C1 to C5. Clean-up steps may be omitted if

interference of matrix load during analysis with chromatographic methods described in modules D1 to

D6 is not evident. In some cases clean-up could be replaced by dilution of the raw extracts (module C0).

Prior to the determination usually some stabilization of the extracts is performed (module S1). All

modules are described in detail in Annex A. Complementary information is given in Annex B.

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SIST EN 15662:2018
EN 15662:2018 (E)

Tables 1 to 4 contain brief descriptions of the modules as well as application notes and examples of use.

For the calculation of residue concentrations in the sample extracts all of the calibration procedures and

quantification methods described in options Q1 to Q7 (Table 5) are applicable. Preferred combinations

of modules concerning the extraction of samples and clean-up of raw extracts are listed in Table 6 for a

multitude of commodities (raw as well as processed).
Table 1 — Extraction (E)
module Description Preferred application Examples
Extraction without hydrolysis

E1 A test portion of 10 g without Plant material and edibles with Fruit and vegetables,

any addition of water is high water content (≥80 %) juices
extracted with acetonitrile

E2 10 g test portion is extracted by Plant material and edibles with (a) Lemons, lime, red

10 ml acetonitrile after addition high water content (≥80 %) and current
of (a) 0,6 ml or (b) 0,2 ml high acid content
(b) raspberry, blackberry
sodium hydroxide solution.

E3 A test portion of 10 g is Plant material and edibles with (a) Bananas, root and

completed with (a) 2,5 ml or (b) intermediate water content tuber vegetables
4,5 ml of water and then (> 40 % and < 80 %) (potatoes, yam, parsnip)
extracted with acetonitrile
(b) Bread, fresh dates,
chestnuts

E4 Test sample is homogenized Plant material and edibles with Dried fruit und similar

with water and a test portion of low water content (15 % to 40 %) commodities
13,5 g of the homogenate is
extracted with acetonitrile.

E5 A test portion of 5 g is Plant material and edibles with Cereal grain and products

completed with 10 ml of water very low water content (<15 %) thereof, honey
and then extracted with and honey
acetonitrile
E6 A test portion of 5 g is Plant material and edibles with Garlic, avocados
completed with 6 ml of water intermediate water content
and then extracted with (>40 % to 80 %) and high matrix
acetonitrile load or high oil content (>5 %)

E7 A test portion of 2 g is Plant material and edibles with Spices, coffee, tobacco,

completed with 10 ml of water very low water content (<15 %) tea, lentils, freeze-dried

and then extracted with and high matrix load as well as fruit
acetonitrile freeze-dried products
Extraction with hydrolysis

E8 Hydrolysis of esters and Plant material and edibles with Fruit and vegetables,

conjugates of acidic pesticides in neutral or acidic pH and high juices, lemons
the slurry of 10 g sample in water content (≥80 %)
acetonitrile followed by
extraction with acetonitrile
(proposed reference test
method for alkaline hydrolysis)

E9 Hydrolysis of esters and Plant material and edibles with Cereal grain and products

conjugates of acidic pesticides in low water content thereof, garlic, spices,
the slurry of 2 g to 5 g sample in coffee, tobacco, tea,
acetonitrile followed by lentils, freeze-dried fruit
extraction with acetonitrile
(proposed reference test
method for alkaline hydrolysis)
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SIST EN 15662:2018
EN 15662:2018 (E)
Table 2 — Clean-up (C)
Module Description Preferred application Examples
C0 No clean-up Base-sensitive and acidic Cucumber, apples,
pesticides (pK < 5) that sufficiently diluted raw-
interact with the amino- extracts
sorbent (PSA) used in
modules C2 to C5, analysis of
extracts with low matrix-load
C1 Freezing-out Removal of co-extracted fat Oranges, lemons, cereal
(even in combination with grain
further clean-up steps, e.g. C2,
C3, C5)
C2 Dispersive SPE with Clean-up of raw-extracts prior Standard-procedure for any
amino-sorbent (PSA) to the determination of basic commodity not shown
and neutral pesticides separately
C3 Dispersive SPE with a Clean-up of raw-extracts of Raw-extracts from modules

larger amount of amino- foods of plant origin with high E5 (e.g. cereal grain and

sorbent (PSA) matrix-load prior to the products thereof) and E7
determination of basic and (e.g. coffee, tea, dried herbs,
neutral pesticides spices)

C4 Dispersive SPE with a Simultaneous clean-up of raw- Citrus fruit, cereal grain and

mixture of amino-sorbent extracts and removal of co- products thereof, avocados,
and silica-based reversed extracted fat olives
phase sorbent (PSA/ODS)

C5 Dispersive SPE with a Clean-up of intensely coloured Iceberg lettuce, head lettuce,

mixture of amino-sorbent raw-extracts prior to the rocket salad
and graphitized carbon determination of basic and
black (PSA/GCB) neutral pesticides
Table 3 — Extract stabilization (S)
Module Description Preferred application Examples
S0 No extract stabilization acid-labile analytes Flazasulfurone,
Mesosulfurone,
Tribenurone,
Triflusulfurone
S1 Extract stabilization with base-labile and acid-stable Majority of analytes
formic acid analytes
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SIST EN 15662:2018
EN 15662:2018 (E)
Table 4 — Detection (D)
Module Description Preferred application Examples
D1 LC-MS/MS Extracts from modules E1 to E9 Analytes ionizable by
subsequently cleaned-up with modules ESI/APCI in extracts from
C0 to C5 any commodity
D2 LC-HR-MS Extracts from modules E1 to E9 Analytes ionizable by
subsequently cleaned-up with modules ESI/APCI in extracts from
C0 to C5 any commodity
D3 GC-MS/MS Extracts from modules E1 to E7 Analytes ionizable by
subsequently cleaned-up with modules EI/PCI/NCI in extracts from
C0 to C5 any commodity
D4 GC-MS (incl. ITD Extracts from modules E1 to E7 Analytes ionizable by
and TOF) subsequently cleaned-up with modules EI/PCI/NCI in extracts from
C0 to C5 commodities with low
matrix-load
D5 GC-FPD Extracts from modules E1 to E7 Organophosphorus and
subsequently cleaned-up with modules sulfur-containing
C1 to C5 compounds
D6 GC-ECD Extracts from modules E1 to E7 Organochlorine compounds
subsequently cleaned-up with modules
C1 to C5

The gas chromatographic determination with single quadrupole mass spectrometric detection

(preferred in SIM mode), with ion trap detectors and with time-of-flight mass spectrometric detection

(independent of the MS resolution) is suited for all analytes. GC-MS analysis without clean-up is only

possible if the extracts are highly diluted (module C0).
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SIST EN 15662:2018
EN 15662:2018 (E)
Table 5 — Quantification (Q)
Option Description Preferred application Reference
Q1 Quantification using Determinations where matrix- see CEN/TS 17061:2017,
external standards in effects are assumed to be 4.4.2 to 4.4.5
solvent negligible
Q2 Quantification using Determinations where matrix- see CEN/TS 17061:2017,
external standards in matrix effects shall be considered 4.3 and 4.4.2 to 4.4.5
Q3 Quantification using a Determinations where matrix- see CEN/TS 17061:2017,
procedural internal effects are assumed to be 4.5.2
standard and standards in negligible
solvent
Q4 Quantification using Determinations where matrix see CEN/TS 17061:2017,
standard addition to the effects shall be considered and 4.6.2
final extract suitable blank matrices are not
available
Q5 Quantification using a Determinations where matrix- see CEN/TS 17061:2017,
procedural internal effects shall be considered for 4.3, 4.5.2 and 4.5.3
standard and standards in compensation of low recovery
matrix or isotope-labelled
internal standards
Q6 Quantification using Determinations where matrix- see CEN/TS 17061:2017,
standard addition to the effects shall be considered 4.6.3
sample without availability of blank
(control) samples or
incomplete extractions of the
analyte occur

Q7 Quantification by calibration Determinations where matrix- see CEN/TS 17061:2017,

of the entire procedure effects shall be considered or 4.7
incomplete extractions of the
analyte occur
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SIST EN 15662:2018
EN 15662:2018 (E)

Table 6 — Preferred combinations of extraction and clean-up modules for particular

commodities
Commodity Extraction Description Clean-up Description Clean-up Description
a b b
(E) (C) (C altern.)
(E) (C) (C altern.)
Apple juice E1 10 g / 0 ml C2 PSA 25 — —
Apple pomace E1 10 g / 0 ml C2 PSA 25 — —
Apples E1 10 g / 0 ml C2 PSA 25 — —
Apples, dried E4 500 g / 850 ml C2 PSA 25 — —
Apricots E1 10 g / 0 ml C2 PSA 25 — —
Apricots, dried E4 500 g / 850 ml C2 PSA 25 — —
Apricot juice E1 10 g / 0 ml C2 PSA 25 — —
Asparagus E1 10 g / 0 ml C2 PSA 25 — —
Aubergine E1 10 g / 0 ml C2 PSA 25 — —
Avocado E6 5 g / 6 ml C1 + C2 Freeze out + C4 PSA 25 + C18
PSA 25 25
Bananas E3a 10 g / 2,5 ml C2 PSA 25 — —
Bananas, freeze-dried E7 2 g / 10 ml C2 PSA 25 — —
Basil E1 10 g / 0 ml C5b PSA 25 +GCB — —
7,5
Bean seeds, fresh E3a 10 g / 2,5 ml C2 PSA 25 — —
Beans, dried E5 5 g / 10 ml C2 PSA 25 — —
Beetroot E1 10 g / 0 ml C2 PSA 25 — —
Blackberries E2b 10 g / NaOH 2 C2 PSA 25 — —
Blackberries, freeze-dried E7 2 g / 10 ml C2 PSA 25 C3a PSA 50
Blueberries E1 10 g / 0 ml C2 PSA 25 — —
Blueberries, dried (14 % E4 500 g / 850 ml C2 PSA 25 — —
water)
Blueberries, freeze-dried E7 2 g / 10 ml C2 PSA 25 C3a PSA 50
Bread (34 % to 43 % E3b 10 g / 4,5 ml C2 PSA 25 — —
water)
Breadfruits (70 % water) E3a 10 g / 2,5 ml C2 PSA 25 — —
Broccoli E1 10 g / 0 ml C2 PSA 25 — —
Carrot E1 10 g / 0 ml C5a PSA 25 + GCB — —
2,5
Carrots, freeze-dried E7 2 g / 10 ml C2 PSA 25 C5a PSA 25 + GCB
2,5
Cauliflower E1 10 g / 0 ml C2 PSA 25 — —
Celeriacs/turnip rooted E1 10 g / 0 ml C2 PSA 25 C5a PSA 25 + GCB
celeries 2,5
Celery E1 10 g / 0 ml C2 PSA 25 — —
Celery, freeze-dried E7 2 g / 10 ml C2 PSA 25 C5b PSA 25 + GCB
7,5
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SIST EN 15662:2018
EN 15662:2018 (E)
Commodity Extraction Description Clean-up Description Clean-up Description
a b b
(E) (C) (C altern.)
(E) (C) (C altern.)
Cereal flour E5 5 g / 10 ml C1 + C3a Freeze out + C4 PSA 25 + C18
PSA 50 25
Cereal grain E5 5 g / 10 ml C1 + C3a Freeze out + C4 PSA 25 + C18
PSA 50 25
Cereal semolina E5 5 g / 10 ml C 1 + C 3a Freeze out + C4 PSA 25 + C18
PSA 50 25
Cereals flakes E5 5 g / 10 ml C1 + C3a Freeze out + C4 PSA 25 + C18
PSA 50 25
Cherries E1 10 g / 0 ml C2 PSA 25 — —
Chestnuts (45 % to 52 % E3b 10 g / 4,5 ml C2 PSA 25 — —
water)
Chinese cabbages E1 10 g / 0 ml C2 PSA 25 — —
Chives E1 10 g / 0 ml C5b PSA 25 + GCB — —
7,5
Chives, freeze-dried E7 2 g / 10 ml C2 PSA 25 C5b PSA 25 + GCB
7,5
Coconut, fresh E6 5 g / 6 ml C2 PSA 25 — —
Coffee beans E7 2 g / 10 ml C3b PSA 75 — —
Coriander E1 10 g / 0 ml C5b PSA 25 + GCB — —
7,5
Corn, freeze-dried E7 2 g / 10 ml C2 PSA 25 — —
Corn, fresh E3a 10 g / 2,5 ml C2 PSA 25 — —
Courgettes E1 10 g / 0 ml C2 PSA 25 — —
Cress E1 10 g / 0 ml C5b PSA 25 + GCB — —
7,5
Cucumber E1 10 g / 0 ml C2 PSA 25 — —
Currants E2a 10 g / NaOH 1 C2 PSA 25 — —
Currants, freeze-dried E7 2 g / 10 ml C2 PSA 25 — —
Currants juice E2a 10 g / NaOH 1 C2 PSA 25 — —
Dates, dried E4 500 g / 850 ml C2 PSA 25 — —
Dates, fresh (50 % to E3b 10 g / 4,5 ml C2 PSA 25 — —
60 % water)
Durian E6 5 g / 6 ml C1 + C2 Freeze out + C4 PSA 25 + C18
PSA 25 25
Escaroles/broad-leaved E1 10 g / 0 ml C5a PSA 25 + GCB — —
endives 2,5
Figs, dried E4 500 g / 850 ml C2 PSA 25 — —
Fungi cultivated E1 10 g / 0 ml C2 PSA 25 — —
Fungi, dried (e.g. Shitake, E5 5 g / 10 ml C2 PSA 25 — —
boletus)
Garlic (59 % water) E6 5 g / 6 ml C2 PSA 25 — —
Ginger (79 % water) E6 5 g / 6 ml C2 PSA 25 — —
---------------------- Page: 13 ----------------------
SIST EN 15662:2018
EN 15662:2018 (E)
Commodity Extraction Description Clean-up Description Clean-up Description
a b b
(E) (C) (C altern.)
(E) (C) (C altern.)
Ginkgo seeds (55 % E3b 10 g / 4,5 ml C2 PSA 25 — —
water)
Globe artichokes E1 10 g / 0 ml C2 PSA 25 — —
Gooseberrys E2b 10 g / NaOH 2 C2 PSA 25 — —
Grape leaves E3a 10 g / 2,5 ml C2 PSA 25 — —
Grape leaves E1 10 g / 0 ml C5b PSA 25 + GCB — —
7,5
Grapefruit E1 10 g / 0 ml C1 + C2 Freeze out + — —
PSA 25
Grapes E1 10 g / 0 ml C2 PSA 25 — —
Head brassica E1 10 g / 0 ml C2 PSA 25 — —
Head lettuce E1 10 g / 0 ml C5a PSA 25 + GCB — —
2,5
Honey E5 5 g / 10 ml C2 PSA 25 — —
Honeydew melon E1 10 g / 0 ml C2 PSA 25 — —
Horseradish E3a 10 g / 2,5 ml C2 PSA 25 — —
Jackfruit (74 % water) E3a 10 g / 2,5 ml C2 PSA 25 — —
Kales E1 10 g / 0 ml C5b PSA 25 + GCB — —
7,5
Kiwi E1 10 g / 0 ml C2 PSA 25 — —
Kohlrabi E1 10 g / 0 ml C2 PSA 25 — —
Lamb's lettuces E1 10 g / 0 ml C5b PSA 25 + GCB — —
7,5
Leek E1 10 g / 0 ml C2 PSA 25 — —
Leek, freeze-dried E7 2 g / 10 ml C2 PSA 25 — —
Lemon grass, fresh (71 % E6 5 g / 6 ml C2 PSA 25 — —
water)
Lemon juice E2a 10 g / NaOH 1 C2 PSA 25 — —
Lemons E2a 10 g / NaOH 1 C1 + C2 Freeze out + — —
PSA 25
Lentils, dried E5 5 g / 10 ml C2 PSA 25 — —
Lime juice E2a 10 g / NaOH 1 C2 PSA 25 — —
Limes E2a 10 g / NaOH 1 C1 + C2 Freeze out + — —
PSA 25
Lotus roots (79 % water) E3a 10 g / 2,5 ml C2 PSA 25 — —
Lotus seeds E3a 10 g / 2,5 ml C2 PSA 25 — —
Lotus seeds, dried (14 % E5 5 g / 10 ml C2 PSA 25 — —
water)
Mandarins E1 10 g / 0 ml C1 + C2 Freeze out + — —
PSA 25
Mango E1 10 g / 0 ml C5a PSA 25 + GCB — —
2,5
---------------------- Page: 14 ----------------------
SIST EN 15662:2018
EN 15662:2018 (E)
Commodity Extraction Description Clean-up Description Clean-up Description
a b b
(E) (C) (C altern.)
(E) (C) (C altern.)
Mango, freeze-dried E7 2 g / 10 ml C2 PSA 25 C5b PSA 25 + GCB
7,5
Mirabelle E1 10 g / 0 ml C2 PSA 25 — —
Nectarines E1 10 g / 0 ml C2 PSA 25 —
...

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