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ASTM E3364-22

(Test Method)

Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Porous and Nonporous Materials Against Staining by Streptomyces species (A Pink Stain Organism)

Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Porous and Nonporous Materials Against Staining by <emph type="ital" >Streptomyces</emph> species (A Pink Stain Organism)

SIGNIFICANCE AND USE
5.1 Methods such as D3273 Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber and D3274 Standard Test Method for Evaluating the Degree of Surface Disfigurement of Paint Films by Fungal or Algal Growth or Soil or Dirt Accumulation provide means for assessing mold and algal staining on paints. The Test Method E1428 Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by Streptomyces species (A Pink Stain Organism) is used for solid polymeric materials, but is not appropriate for all antimicrobial technologies.  
5.2 This test method provides a technique for evaluating antimicrobials in or on polymeric materials against staining by Streptomyces species and should assist in the prediction of performance of treated articles under actual field conditions.
SCOPE
1.1 This test method is intended to assess susceptibility of polymer materials, as well as products that may directly contact the treated polymer, to staining by the Actinomycete Streptomyces species.  
1.2 This test method is also suitable for evaluating dark-pigmented test samples since the bacterial growth inhibition can be assessed.  
1.3 Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Published
Publication Date
30-Sep-2022
ICS
07.100.99 - Other standards related to microbiology
Technical Committee
E35 - Pesticides, Antimicrobials, and Alternative Control Agents
Drafting Committee
E35.15 - Antimicrobial Agents
Current Stage
Ref Project

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ASTM E3364-22 - Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Porous and Nonporous Materials Against Staining by <emph type="ital" >Streptomyces</emph> species (A Pink Stain Organism)ASTM E3364-22 - Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Porous and Nonporous Materials Against Staining by <emph type="ital" >Streptomyces</emph> species (A Pink Stain Organism)
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ASTM E3364-22 - Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Porous and Nonporous Materials Against Staining by <emph type="ital" >Streptomyces</emph> species (A Pink Stain Organism)
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E3364 − 22
Standard Test Method for
Evaluating the Performance of Antimicrobials in or on
Polymeric Porous and Nonporous Materials Against
1
Staining by Streptomyces species (A Pink Stain Organism)
This standard is issued under the fixed designation E3364; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
When certain bacteria and mold species grow on the surface of non-rigid, flexible or “plasticized”
polymers, metabolites such as pigments in the case of certain bacteria and melanin (dark stains from
fungal growth) cause undesirable stains on the polymer surface. These stains may persist even after
the surface growth is removed.This test method is used to determine the performance of antimicrobial
agents used in or on synthetic polymeric porous and non-porous materials against staining by the
Actinomycete, Streptomyces species. This organism has been chosen as an indicator organism,
although other organisms have been known to cause undesirable staining in polymeric porous and
non-porous materials.
1. Scope mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
1.1 This test method is intended to assess susceptibility of
polymer materials, as well as products that may directly
2. Referenced Documents
contact the treated polymer, to staining by the Actinomycete
2
Streptomyces species. 2.1 ASTM Standards:
D3273 TestMethodforResistancetoGrowthofMoldonthe
1.2 This test method is also suitable for evaluating dark-
Surface of Interior Coatings in an Environmental Cham-
pigmented test samples since the bacterial growth inhibition
ber
can be assessed.
D3274 Test Method for Evaluating Degree of Surface Dis-
1.3 Familiarity with microbiological techniques is required.
figurement of Paint Films by Fungal or Algal Growth, or
This test method should not be used by persons without at least
Soil and Dirt Accumulation
basic microbiological training.
E177 Practice for Use of the Terms Precision and Bias in
ASTM Test Methods
1.4 The values stated in SI units are to be regarded as
E2756 Terminology Relating to Antimicrobial and Antiviral
standard. No other units of measurement are included in this
Agents
standard.
E1428 Test Method for Evaluating the Performance of
1.5 This standard does not purport to address all of the
Antimicrobials in or on Polymeric SolidsAgainst Staining
safety concerns, if any, associated with its use. It is the
by Streptomyce species (A Pink Stain Organism)
responsibility of the user of this standard to establish appro-
priate safety, health, and environmental practices and deter-
3. Terminology
mine the applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accor-
3.1 Defintions:
dance with internationally recognized principles on standard- 3.1.1 For definitions of terms used in this method, refer to
ization established in the Decision on Principles for the
Terminology E2756.
Development of International Standards, Guides and Recom-
3.2 Definitions of Terms Specific to This Standard:
1
This test method is under the jurisdiction of ASTM Committee E35 on
2
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct For referenced ASTM standards, visit the ASTM website, www.astm.org, or
responsibility of Subcommittee E35.15 on Antimicrobial Agents. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Current edition approved Oct. 1, 2022. Published November 2022. DOI: Standards volume information, refer to the standard’s Document Summary page on
10.1520/E3364–22 the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E3364 − 22
3.2.1 microbially induced staining, n—undesirable pigmen- 7.5 Sterilizer, suitable for the substrate to be tested, that is,
tation or disfiguration of an object due to surface colonization 70 % isopropanol (optional).
by certain microorganisms. Both bacteria and fungi produce
7.6 Parafilm, sealable plastic bags or equivalent to prevent
metabolic pigments that can result in surface stains on suscep-
moisture loss.
tible objects.
7.7 Spreader, sterile.
3.2.2 pink stain organism, n—an Actinomycete such as
7.8 Waterbath, capable of maintaining water at 47 °C 6
Streptomyces species ATCC 25607 (deposited as Streptoverti-
2 °C.
cillium reticulum) t
...

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