EN ISO 12966-1:2014

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966-1:2014)

ISO 12966-1:2014 gives an overview of the gas chromatographic determination of fatty acids, free and bound, in animal and vegetable fats and oils following their conversion to fatty acid methyl esters (FAMEs).

Tierische und pflanzliche Fette und Öle - Gaschromatographie von Fettsäuremethylestern - Teil 1: Leitfaden für die moderne Gaschromatographie von Fettsäuremethylestern (ISO 12966-1:2014)

Dieser Teil der ISO 12966 gibt einen Überblick über die gaschromatographische Bestimmung von freien und gebundenen Fettsäuren in tierischen und pflanzlichen Fetten und Ölen, die deren Umwandlung zu Fettsäure-methylestern (FAME) folgt.
Die qualitative und quantitative Bestimmung der Zusammensetzung von Fettsäuren mit der Gas-flüssig-Chromatographie (GLC) ist ein häufig angewendetes Verfahren bei der Lipidanalyse. Es wird zur Charakterisierung von Fetten und Ölen oder fetthaltigen Lebensmitteln nach der Extraktion des Öls aus der Matrix angewendet. Die gebundenen Fettsäuren der Triacylglycerine (TAG) und, in Abhängigkeit vom Ver-fahren der Veresterung, die freien Fettsäuren (FFA) und andere Lipide werden zu Fettsäuremethylestern (FAME) umgewandelt, die mit der Kapillar-Gaschromatographie bestimmt werden. Abhängig von der Anzahl der unterschiedlichen Fettsäuren (theoretisch können mehr als 50 verschiedene Fettsäuren vorliegen) werden für eine Trennung Kapillarsäulen mit einer Länge von 10 m bis 100 m verwendet.
Die GLC von FAME ist für natürliche und synthetische Gemische von Tri-, Di- und Monoacylglycerinen, für Fettsäureester, freie Fettsäuren, Seifen und weitere Fettverbindungen geeignet. Mit dieser Normenreihe können FAME von C4 bis C26 einschließlich gesättigter Fettsäuremethylester, einfach ungesättigter cis- und trans-Fettsäuremethylester und mehrfach ungesättigter cis- und trans-Fettsäuremethylester bestimmt werden.
Für die Bestimmung kurzkettiger Fettsäuren werden häufig Isopropyl- und Butylester verwendet, um damit Interferenzen mit dem Lösemittelpeak zu vermeiden und um die Differenzen in den Detektorresponsen zu reduzieren.

Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des esters méthyliques d'acides gras - Partie 1: Lignes directrices relatives à la chromatographie en phase gazeuse moderne des esters méthyliques d'acides gras (ISO 12966-1:2014)

L'ISO 12966-1:2014 donne un aperçu de la détermination par chromatographie en phase gazeuse des acides gras, libres et liés, dans les corps gras d'origines animale et végétale après leur conversion en esters méthyliques d'acides gras (EMAG).

Živalske in rastlinske maščobe in olja - Plinska kromatografija metilnih estrov maščobnih kislin - 1. del: Smernice za sodobno plinsko kromatografijo metilnih estrov maščobnih kislin (ISO 12966-1:2014)

Ta del standarda ISO 12966 podaja pregled določanja maščobnih kislin, prostih in vezanih, s plinsko kromatografijo v živalskih ter rastlinskih maščobah in oljih po njihovi pretvorbi v metilne estre maščobnih kislin (FAMEs).
Kvalitativno in kvantitativno določanje sestave maščobnih kislin s plinsko-tekočinsko kromatografijo (GLC) se pogosto uporablja pri analizi maščobnih kislin. Uporablja se za karakterizacijo maščob in olj ali maščobnih živil po ekstrakciji olja iz matrice. Vezane maščobne kisline triacilglicerolov (TAGs) in, odvisno od metode zaestrenja, proste maščobne kisline (FFA) ter druge maščobe so pretvorjene v metilne estre maščobnih kislin (FAMEs), ki se določijo s plinsko kromatografijo s kapilarnimi kolonami. Glede na število različnih maščobnih kislin (teoretično je lahko prisotnih več kot 50 različnih maščobnih kislin) se za separacijo uporabljajo kapilarne kolone z dolžino od 10 m do 100 m. Plinsko-tekočinska kromatografija metilnih estrov maščobnih kislin se uporablja pri vseh naravnih in sintetičnih mešanicah tri-, di- in monoacilglicerolov z estri maščobnih kislin, prostimi maščobnimi kislinami, mili in drugimi maščobnimi sestavinami. S temi standardi je mogoče določiti metilne estre maščobnih kislin od C4 do C26, vključno z nasičenimi metilnimi estri maščobnih kislin, mononenasičenimi metilnimi estri maščobnih kislin v cis in trans obliki ter večkrat nenasičenimi metilnimi estri maščobnih kislin v cis in trans obliki. Za določanje kratkoverižnih maščobnih kislin se pogosto uporabljajo izopropilni in butilni estri, da se prepreči vrhe topila in zmanjša razlike pri odzivih detektorja.

General Information

• Status: Published
• Publication Date: 02-Dec-2014
• Withdrawal Date: 29-Jun-2015
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 03-Dec-2014
• Completion Date: 03-Dec-2014
• Ref Project: SIST EN ISO 12966-1:2015 - Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966-1:2014)

Relations

• Replaces: EN ISO 5508:1995 - Animal and vegetable fats and oils - Analysis by gas chromatography of methyl esters of fatty acids (ISO 5508:1990)
• Effective Date: 08-Jun-2022
• Replaces: EN ISO 15304:2002 - Animal and vegetable fats and oils - Determination of the content of trans fatty acid isomers of vegetable fats and oils - Gas chromatographic method (ISO 15304:2002)
• Effective Date: 08-Jun-2022
• Replaces: EN ISO 15304:2002/AC:2004 - Animal and vegetable fats and oils - Determination of the content of trans fatty acid isomers of vegetable fats and oils - Gas chromatographic method (ISO 15304:2002/Cor.1:2003)
• Effective Date: 22-Apr-2015
• Corrected By: EN ISO 12966-1:2014/AC:2015 - Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966-1:2014)
• Effective Date: 08-Apr-2015

Standards Content (Sample)

SLOVENSKI STANDARD
01-marec-2015
1DGRPHãþD
SIST EN ISO 15304:2002
SIST EN ISO 15304:2002/AC:2005
SIST EN ISO 5508:1996
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD3OLQVNDNURPDWRJUDILMDPHWLOQLKHVWURY
PDãþREQLKNLVOLQGHO6PHUQLFH]DVRGREQRSOLQVNRNURPDWRJUDILMRPHWLOQLK
HVWURYPDãþREQLKNLVOLQ,62
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters -
Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966
-1:2014)
Tierische und pflanzliche Fette und Öle - Gaschromatographie von
Fettsäuremethylestern - Teil 1: Leitfaden für die moderne Gaschromatographie von
Fettsäuremethylestern (ISO 12966-1:2014)
Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des
esters méthyliques d'acides gras - Partie 1: Directive de chromatographie en phase
gazeuse moderne des esters méthyliques d'acides gras (ISO 12966-1:2014)
Ta slovenski standard je istoveten z: EN ISO 12966-1:2014
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 12966-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2014
ICS 67.200.10 Supersedes EN ISO 5508:1995
English Version
Animal and vegetable fats and oils - Gas chromatography of fatty
acid methyl esters - Part 1: Guidelines on modern gas
chromatography of fatty acid methyl esters (ISO 12966-1:2014)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle -
Chromatographie en phase gazeuse des esters Gaschromatographie von Fettsäuremethylestern - Teil 1:
méthyliques d'acides gras - Partie 1: Lignes directrices Leitfaden für die moderne Gaschromatographie von
relatives à la chromatographie en phase gazeuse moderne Fettsäuremethylestern (ISO 12966-1:2014)
des esters méthyliques d'acides gras (ISO 12966-1:2014)
This European Standard was approved by CEN on 20 October 2014.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12966-1:2014 E
worldwide for CEN national Members.

Contents Page
Foreword .3
Foreword
This document (EN ISO 12966-1:2014) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils
and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by June 2015, and conflicting national standards shall be withdrawn at
the latest by June 2015.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 5508:1995.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 12966-1:2014 has been approved by CEN as EN ISO 12966-1:2014 without any modification.

INTERNATIONAL ISO
STANDARD 12966-1
First edition
2014-12-01
Animal and vegetable fats and oils —
Gas chromatography of fatty acid
methyl esters —
Part 1:
Guidelines on modern gas
chromatography of fatty acid methyl
esters
Corps gras d’origines animale et végétale — Chromatographie en
phase gazeuse des esters méthyliques d’acides gras —
Partie 1: Lignes directrices relatives à la chromatographie en phase
gazeuse moderne des esters méthyliques d’acides gras
Reference number
ISO 12966-1:2014(E)
©
ISO 2014
ISO 12966-1:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Published in Switzerland
ii © ISO 2014 – All rights reserved

ISO 12966-1:2014(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Principle . 1
4 Preparation of FAME . 2
5 Columns . 3
6 GLC of FAMEs . 4
7 Evaluation of the chromatograms . 4
7.1 Peak area and area per cent . 4
7.2 Evaluation by means of an internal standard or correction factors . 4
8 Test report . 5
Bibliography . 6
ISO 12966-1:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
This first edition of ISO 12966-1, together with ISO 12966-4, cancels and replaces ISO 5508:1990 and
ISO 15304:2002 which have been technically revised.
ISO 12966 consists of the following parts, under the general title Animal and vegetable fats and oils — Gas
chromatography of fatty acid methyl esters:
— Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters
— Part 2: Preparation of methyl esters of fatty acids
— Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)
— Part 4: Capillary gas chromatographic method
iv © ISO 2014 – All rights reserved

ISO 12966-1:2014(E)
Introduction
This part of ISO 12966 is one of a suite of four International Standards for the preparation and
determination of fatty acid methyl esters by gas chromatography in animal and vegetable fats and oils.
ISO 12966 (all parts) is applicable to crude, refined, partially hydrogenated or fully hydrogenated fats,
oils and fatty acids derived from animal and vegetable sources.
ISO 12966 (all parts) is not suitable for the analysis of dairy, ruminant fats and oils (including milk and
milk products or fat coming from milk and milk products), or products supplemented with conjugated
linoleic acid (CLA). Furthermore it is not intended to be applied to polymerized and oxidized fats and
oils.
This part of ISO 12966 is a guideline to the modern gas chromatography of fatty acid methyl esters, while
ISO 12966-2 and ISO 12966-3 cover the preparation of fatty acid methyl esters by different methods. In
ISO 12966-4, the conditions for the analysis of fatty acid methyl esters by capillary gas chromatography
are given.
This suite of International Standards replaces the following International Standards:
— ISO 5508:1990 is replaced by ISO 12966-1 and ISO 12966-4
— ISO 15304:2002 is replaced by ISO 12966-1 and ISO 12966-4
— ISO 5509:2000 is replaced by ISO 12966-2 and ISO 12966-3
INTERNATIONAL STANDARD ISO 12966-1:2014(E)
Animal and vegetable fats and oils — Gas chromatography
of fatty acid methyl esters —
Part 1:
Guidelines on modern gas chromatography of fatty acid
methyl esters
1 Scope
This part of ISO 12966 gives an overview of the gas chromatographic determination of fatty acids, free
and bound, in animal and vegetable fats and oils following their conversion to fatty acid methyl esters
(FAMEs).
The qualitative and quantitative determination of the composition of fatty acids by gas liquid
chromatography (GLC) is a widely used application in lipid analysis. It is used for the characterization of
fats and oils, or fatty foodstuffs after the extraction of t
...