EN ISO 20743:2013
(Main)Textiles - Determination of antibacterial activity of textile products (ISO 20743:2013)
Textiles - Determination of antibacterial activity of textile products (ISO 20743:2013)
ISO 20743:2013 specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.
ISO 20743:2013 is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).
Based on the intended application and on the environment in which the textile product is to be used and also on the surface properties of the textile properties, the user can select the most suitable of the following three inoculation methods on determination of antibacterial activity:
a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);
b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);
c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).
The colony plate count method and the ATP (ATP = Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.
Textilien - Bestimmung der antibakteriellen Wirksamkeit von textilen Produkten (ISO 20743:2013)
Die vorliegende Internationale Norm legt quantitative Prüfverfahren zur Bestimmung der antibakteriellen Wirkung antibakteriell behandelter textiler Erzeugnisse, einschließlich Vliesstoffe, fest.
Diese Internationale Norm ist anwendbar auf alle textilen Erzeugnisse, einschließlich Stoffe, Wattierung, Garn und Materialien für Bekleidung, Heimtextilien sowie verschiedene Waren, ungeachtet des Typs des verwendeten antibakteriellen Mittels (organisch, anorganisch, natürlich oder künstlich) oder des Anwendungs-verfahrens (eingebaut, Nachbehandlung oder Pfropfen).
Auf der Grundlage der beabsichtigten Anwendung und der Umgebung, für die das textile Erzeugnis zu verwenden ist, kann der Anwender aus den folgenden drei Verfahren zur Bestimmung der antibakteriellen Wirkung das geeignetste auswählen.
a) Absorptionsverfahren (ein Bewertungsverfahren, bei dem die bakterielle Prüfsuspension direkt auf Proben geimpft wird);
b) Übertragungsverfahren (ein Bewertungsverfahren, bei dem Prüfbakterien auf eine Agarplatte aufgebracht und auf Proben übertragen werden);
c) Stempelverfahren (ein Bewertungsverfahren, bei dem Prüfbakterien auf ein Filter aufgebracht und auf Proben gestempelt werden).
Das Kolonie-Plattenzählverfahren und das ATP-Lumineszenzverfahren (ATP = Adenosintriphosphat) werden auch zum Messen der Bakterienzahl festgelegt.
Textiles - Détermination de l'activité antibactérienne des produits textiles (ISO 20743:2013)
L'ISO 20743:2013 spécifie des méthodes d'essai quantitatives permettant de déterminer l'activité antibactérienne de tous les produits textiles antibactériens, y compris les nontissés.
L'ISO 20743:2013 s'applique à tous les produits textiles, y compris l'étoffe, le rembourrage, le fil et les matériaux utilisés pour les vêtements, la literie, l'ameublement et divers articles, quel que soit le type d'agent antibactérien utilisé (organique, inorganique, naturel ou synthétique) ou quelle que soit la méthode d'application (intégration, post-traitement ou greffage).
Tenant compte de l'application prévue et de l'environnement dans lequel le produit textile est destiné à être utilisé, et également des propriétés de surface du textile, l'utilisateur peut choisir la plus adaptée des trois méthodes d'ensemencement suivantes pour la détermination de l'activité antibactérienne:
a) méthode par absorption (méthode d'évaluation dans laquelle la suspension bactérienne d'essai est ensemencée directement sur des éprouvettes);
b) méthode par transfert (méthode d'évaluation dans laquelle les bactéries d'essai sont placées sur une boîte de milieu gélosé, puis transférées sur des éprouvettes);
c) méthode par impression (méthode d'évaluation dans laquelle les bactéries d'essai sont placées sur un filtre, puis imprimées sur des éprouvettes).
La technique de dénombrement et la méthode de mesure par luminescence de l'ATP (adénosine triphosphate) sont également spécifiées pour le dénombrement des bactéries.
Tekstilije - Ugotavljanje protibakterijske aktivnosti na tekstilnih izdelkih (ISO 20743:2013)
Ta mednarodni standard določa kvantitativne preskusne metode za ugotavljanje protibakterijske aktivnosti na vseh tekstilnih izdelkih, vključno z vlaknovinami. Ta mednarodni standard se uporablja za vse tekstilne izdelke, vključno z blagom, vato, nitmi in materialom za oblačila, izdelki za opremljanje domov in drugo, ne glede na vrsto uporabljene protibakterijske učinkovine (organskega, neorganskega, naravnega ali kemičnega izvora) ali način uporabe (vgrajeno, naknadna obdelava ali cepljenje). Na podlagi nameravane uporabe in okolja, v katerem se bo tekstilni izdelek uporabljal, lahko uporabnik izbere najbolj primerno izmed naslednjih treh metod za določanje protibakterijske aktivnosti: a) metodo absorpcije (metoda ocenjevanja, pri kateri je testna suspenzija bakterij vcepljena neposredno na vzorce); b) metoda prenosa (metoda ocenjevanja, pri kateri se testne bakterije razlijejo na ploščo gojišča in prenesejo na vzorce); c) metoda tiskanja (metoda ocenjevanja, pri kateri se testne bakterije razlijejo na filter in natisnejo na vzorce). Metoda za štetje kolonij in luminescenčna metoda z ATP-jem (ATP = adenozin trifosfat) sta tudi določeni za merjenje števila bakterij.
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2013
1DGRPHãþD
SIST EN ISO 20743:2007
Tekstilije - Ugotavljanje protibakterijske aktivnosti na tekstilnih izdelkih (ISO
20743:2013)
Textiles - Determination of antibacterial activity of textile products (ISO 20743:2013)
Textilien - Bestimmung der antibakteriellen Wirksamkeit von textilen Produkten (ISO
20743:2013)
Textiles - Détermination de l'activité antibactérienne des produits textiles (ISO
20743:2013)
Ta slovenski standard je istoveten z: EN ISO 20743:2013
ICS:
07.100.99 Drugi standardi v zvezi z Other standards related to
mikrobiologijo microbiology
59.080.01 Tekstilije na splošno Textiles in general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 20743
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2013
ICS 07.100.99; 59.080.01 Supersedes EN ISO 20743:2007
English Version
Textiles - Determination of antibacterial activity of textile
products (ISO 20743:2013)
Textiles - Détermination de l'activité antibactérienne des Textilien - Bestimmung der antibakteriellen Wirkung
produits textiles (ISO 20743:2013) antibakteriell behandelter Erzeugnisse (ISO 20743:2013)
This European Standard was approved by CEN on 22 June 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20743:2013: E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (EN ISO 20743:2013) has been prepared by Technical Committee ISO/TC 38 "Textiles" in
collaboration with Technical Committee CEN/TC 248 “Textiles and textile products” the secretariat of which is
held by BSI.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2014, and conflicting national standards shall be withdrawn at
the latest by January 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 20743:2007.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 20743:2013 has been approved by CEN as EN ISO 20743:2013 without any modification.
INTERNATIONAL ISO
STANDARD 20743
Second edition
2013-07-15
Textiles — Determination of
antibacterial activity of textile products
Textiles — Détermination de l’activité antibactérienne des produits
textiles
Reference number
ISO 20743:2013(E)
©
ISO 2013
ISO 20743:2013(E)
© ISO 2013
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ISO 20743:2013(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Safety precaution . 2
5 Apparatus . 2
6 Reagents and culture media . 4
6.1 Water . 4
6.2 Tryptone soya broth (TSB) . 4
6.3 Tryptone soya agar (TSA) . 4
6.4 Agar for transfer . 5
6.5 Nutrient broth (NB) . 5
6.6 Peptone salt solution . 5
6.7 Physiological saline . 5
6.8 SCDLP medium . 6
6.9 Dilution buffer for shake-out bacterial suspension. 6
6.10 Neutralizing solution . 6
6.11 Enumeration agar (EA) . 7
6.12 Agar for printing . 7
6.13 Cryoprotective solution for bacterial species . 7
6.14 Stock solution of ATP standard reagent . 7
6.15 Buffer solution for ATP luminescent reagent . 8
6.16 ATP luminescent reagent . 8
6.17 ATP extracting reagent . 8
6.18 ATP eliminating reagent . 8
6.19 SCDLP or other medium for preparing ATP reference solution . 9
6.20 Shake-out physiological saline . 9
7 Reference strains . 9
7.1 Strains . 9
7.2 Storage of strains .10
8 Test procedures .11
8.1 Absorption method (see Annex E) .11
8.2 Transfer method (see Annex E) .15
8.3 Printing method (see Annex E) .18
9 Test report .22
Annex A (normative) Strain numbers .23
Annex B (normative) Shaking method .24
Annex C (normative) Quantitative measurement by plate count method .25
Annex D (normative) Quantitative measurement by luminescence method .26
Annex E (informative) Testing examples .28
Annex F (informative) Efficacy of antibacterial activity .31
Bibliography .32
ISO 20743:2013(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take p
...
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