Characterization of waste - Preparation of waste samples for ecotoxicity tests

Charakterisierung von Abfällen - Herstellung von Abfallproben für ökotoxikologische Untersuchungen

Caractérisation des déchets - Préparation des échantillons de déchets en vue d'essais écotoxicologiques

Karakterizacija odpadkov - Priprava vzorcev odpadka za ekotoksikološke preskuse - Popravek AC

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SIST EN 14735:2005/AC:2007
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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Characterization of waste - Preparation of waste samples for ecotoxicity testsKarakterizacija odpadkov - Priprava vzorcev odpadka za ekotoksikološke preskuse - Popravek ACCaractérisation des déchets - Préparation des échantillons de déchets en vue d'essais écotoxicologiquesCharakterisierung von Abfällen - Herstellung von Abfallproben für ökotoxikologische UntersuchungenTa slovenski standard je istoveten z:EN 14735:2005/AC:2006SIST EN 14735:2005/AC:2007en,de13.030.01Odpadki na splošnoWastes in generalICS:SLOVENSKI

STANDARDSIST EN 14735:2005/AC:200701-april-2007

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14735:2005/ACJune 2006Juin 2006Juni 2006ICS 13.030.01English versionVersion FrançaiseDeutsche FassungCharacterization of waste - Preparation of waste samples for ecotoxicitytestsCaractérisation des déchets - Préparationdes échantillons de déchets en vued'essais écotoxicologiquesCharakterisierung von Abfällen -Herstellung von Abfallproben fürökotoxikologische UntersuchungenThis corrigendum becomes effective on 14 June 2006 for incorporation in the three official languageversions of the EN.Ce corrigendum prendra effet le 14 juin 2006 pour incorporation dans les trois versions linguistiquesofficielles de la EN.Die Berichtigung tritt am 14.Juni 2006 zur Einarbeitung in die drei offiziellen Sprachfassungen der ENin Kraft.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36

B-1050 Brussels© 2006 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members.Tous droits d'exploitation sous quelque forme et de quelque manière que ce soit réservés dans le monde entier auxmembres nationaux du CEN.Alle Rechte der Verwertung, gleich in welcher Form und in welchem Verfahren, sind weltweit den nationalen Mitgliedernvon CEN vorbehalten.Ref. No.:EN 14735:2005/AC:2006 D/E/F

EN 14735:2005/AC:2006 (E/F/D) 2 Introduction Delete the numbering of clauses: 0, 0.1 and 0.2. Replace the last sentence by: This European Standard describes the necessary steps to be performed before carrying out ecotoxicity tests on wastes within the context of assessment of ecotoxic properties for classification purposes.

3.1 Replace the definition 3.1 by: 3.1 dilution medium liquid or solid used for the preparation of control vessels and the preparation of test mixtures

3.8 Replace the definition 3.8 by: 3.8 liquid sludge sludge that has the characteristic of a liquid as specified in the definition of a liquid waste (3.9)

5 Taking of laboratory sample Replace the NOTE by: NOTE Sampling devices are described in a Technical Report under development (prCEN/TR 15310-2).

6 Transport Replace the last sentence by: A transport time of less than 48 h and/or a low temperature conditions (4 ± 2) °C are appropriate to maintain the properties of laboratory samples.

7.2 Waste sample Replace second paragraph by: A storage time of less than two months and low temperature conditions (4 ± 2) °C are appropriate to maintain the properties of waste samples.

EN 14735:2005/AC:2006 (E/F/D) 3 8 Waste characterization Replace the NOTE by: NOTE The method described in Annex A of ISO 14238:1997 has been found to be appropriate for most kinds of waste.

11.2.1 Leaching procedure Replace the third paragraph by : Ecotoxicological tests usually require several litres of eluate leading to an adjustment of mass of the test portion. When a large volume of eluate is required, it is possible to perform the leaching test in a vessel of appropriate capacity into which the corresponding number of test portions [a dry mass of (90 ± 5) g] are introduced or to perform parallel leaching tests at the same time with an appropriate number of vessels of 1 l capacity each (see above). In that case, all the individual eluates shall be mixed in order to obtain the test sample. When the leaching test is performed in one vessel, the size of the vessel shall be appropriate to minimise the headspace (≤ 5 % of the total capacity of the vessel).

EN 14735:2005/AC:2006 (E/F/D) 4 B.2.4 Coleoptera – Acute test Replace B.2.4 by: 1. Title of the test Effects of pollutants on insect larvae (Oxythyrea funesta) – Determination of acute toxicity using artificial soil substrate 2. Harmonization International 3. References ISO 20963; NF X 31-260 4. Principle Determination of the percent mortality of Cetoniinae larvae during growth period after 10 days 5. Test type Static acute 6. Test organism Third instar larvae of the species Oxythyrea funesta

Breeding stocks
Age ≈ 15 days, wet mass 100 mg to 200 mg
Feeding Dried and finely ground cow-dung 7. Dilution medium Artificial soil
Volume 300 g (dry weight) /container 8. Test conditions
Test chamber Enclosures
Temperature (26 ± 1) °C
PH 6,0 ± 0,5
Light intensity/quality Darkness
Photoperiod -

Soil moisture 50 % of total WHC 9. Number of replicates Three (10 larvae per replicate) 10. Test duration/incubation 10 days 11. Control Artificial Soil 12. Validity criteria Control: mortality < 10 %, biomass increase in the controls > 80 % 13. Reference substance ISO 20963: 2,4,5 trichlorophenol, 60 ≤≤≤≤ LC 50 ≤≤≤≤ 180 mg/kg

NF X31-260:HgCl

2, 15 mg/kg ≤≤≤≤ LC 50 ≤≤≤≤ 45 mg/kg (as Hg2+) 14. Statistics Multiple t-test, u-test, regression analysis 15. Test parameter(s) Mortality, growth (optional) 16. Endpoints LC 50, NOEC (ECx) 17. Application to wastes and water extracts from wastes: limitations and comments The test organism is not sensitive to modifications of the test mixture granulometry.

EN 14735:2005/AC:2006 (E/F/D) 5 B.3.2 Daphnia magna – Inhibition of reproduction Replace B.3.2 by: 1. Title of the test Determination of long term toxicity of substances to Daphnia magna Straus (Cladocera crustacea) 2. Harmonisation International 3. References ISO 10706 ; OECD guideline n°211 4. Principle Inhibition of reproduction and survival of Daphnia magna 5. Test type Chronic, semi-static 6. Test organism Daphnia magna from the second to the fifth brood obtained by acyclical parthenogenesis

Breeding stock Daphnia magna Straus
Age of test organism < 24h

Feeding Unicellular algae (Chlorella sp., Pseudokirchneriella subcapitata or Scenedesmus subspicatus). 0,1 mg carbon/animal/day to 0,2 mg carbon/animal/day 7. Dilution medium ELENDT M4 or M7 synthetic medium ; ASTM reconstituted hard freshwater

Volume 50 ml to 100 ml 8. Test conditions
Test chamber size 50 ml to - 100 ml beakers
Temperature Within 18 °C to 22 °C, variations within less than 2 °C
pH 6,0 to 9,0
Light intensity/quality < 1 200 lux

Photoperiod 16 h light 9. Number of container, number of replicates 5 concentrations × 10 replicates (one animal per vessel is recommended) 10. Test duration 21 days 11. Negative control dilution ELENDT M4 or M7 ; ASTM 12. Validity criteria Mortality of adults or living males ≤ 20 % in the control, mean number of living offspring per parent ≥ 60 in the control, coefficient of variance for control fecundity < 20 % 13. Positive control/reference toxicant, mean EC (and CV) The Daphnid culture may be controlled using K2Cr2O7 (effect on mobility) 14. Statistics Dunnett or Williams test and regression 15. Test parameter Mortality of adults, inhibition of reproduction 16. End points ECx, NOEC 17. Application to wastes and water extracts from wastes: limitations and comments  This test is mainly


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