SIST EN ISO 9308-1:2014/A1:2017

Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016)

Wasserbeschaffenheit Zählung von Escherichia coli und coliformen Bakterien Teil 1: Membranfiltrationsverfahren für Wässer mit niedriger Begleitflora (ISO 9308-1:2014/Amd 1:2016)

Qualité de l'eau - Dénombrement des Escherichia coli et des bactéries coliformes - Partie 1: Méthode par filtration sur membrane pour les eaux à faible teneur en bactéries (ISO 9308-1:2014/Amd 1:2016)

Kakovost vode - Ugotavljanje števila Escherichia coli in koliformnih bakterij - 1. del: Metoda membranske filtracije za vode z majhnim številom spremljajočih bakterij - Dopolnilo A1 (ISO 9308-1:2014/Amd 1:2016)

General Information

• Status: Published
• Public Enquiry End Date: 05-Apr-2016
• Publication Date: 16-May-2017
• ICS: 07.100.20 - Microbiology of water
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: KAV - Water quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 11-Apr-2017
• Due Date: 16-Jun-2017
• Completion Date: 17-May-2017
• Directive: 2008-01-0974 - Uredba o upravljanju kakovosti kopalnih voda
• Directive: 2014-01-3849 - Pravilnik o prvih meritvah in obratovalnem monitoringu odpadnih voda
• Directive: TP213 - Pravilnik o minimalnih higienskih zahtevah, ki jih morajo izpolnjevati kopališča in kopalna voda v bazenih
• Directive: TP262 - Uredba o pitni vodi
• Directive: TRRTP178 - Uredba o upravljanju kakovosti kopalnih voda
• Ref Project: EN ISO 9308-1:2014/A1:2017 - Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016)

Relations

• Amends: SIST EN ISO 9308-1:2014 - Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014)
• Effective Date: 01-Mar-2016

Overview

EN ISO 9308-1:2014/A1:2017 is the CEN-endorsed amendment to ISO 9308-1:2014 and prescribes the membrane filtration method for the enumeration of Escherichia coli and coliform bacteria in waters with low bacterial background flora. The amendment (A1:2016) was approved by CEN as EN ISO 9308-1:2014/A1:2017 and clarifies incubation and agar performance requirements to support consistent laboratory results and regulatory compliance.

Key Topics

• Membrane filtration procedure: The method uses membrane filtration to retain bacterial cells from a water sample before culturing on selective chromogenic media.
• Chromogenic Coliform Agar (CCA): The document specifies placement of membranes on CCA and details performance testing criteria for the medium.
• Incubation conditions: Membranes on CCA are incubated at (36 ± 2) °C for 21 to 24 h (as amended), ensuring reproducible colony development for enumeration.
• CCA performance testing: The amendment replaces the referenced table for CCA performance. Key characteristics include:
  • Productivity: Expected recovery for target strains (example: E. coli) with a productivity ratio (PR) ≥ 0.7.
  • Colony reactions: Typical colour reactions are defined (e.g., E. coli: dark‑blue to violet colonies; Enterobacter aerogenes: pink to red colonies).
  • Selectivity and specificity checks: Inhibition of non‑target organisms and confirmation of colourless colonies for certain non‑coliforms are addressed.
• Reference strains and traceability: The amendment refers to standard culture collection strain numbers for performance verification.

Applications

This standard is essential for laboratories and organizations involved in water quality monitoring, public health surveillance, and regulatory compliance. Typical applications include:

• Routine testing of drinking, recreational and environmental waters where low bacterial background is expected.
• Verification of water treatment efficacy and distribution system surveillance.
• Reference method for compliance testing in jurisdictions that adopt EN ISO 9308-1:2014/A1:2017.

The clear incubation regime and CCA performance criteria help ensure reliable, comparable results across accredited testing facilities.

Related Standards

• ISO 9308-1:2014 - Base international standard for membrane filtration enumeration of E. coli and coliforms.
• EN ISO 9308-1:2014/A1:2017 - CEN endorsement of the ISO 2016 amendment (A1) without modification.
• ISO/TC 147 & CEN/TC 230 - Technical committees responsible for water quality and water analysis methods.

For laboratories and water quality professionals, EN ISO 9308-1:2014/A1:2017 provides a robust, standardized approach to enumerate E. coli and coliforms using membrane filtration and chromogenic media, supporting actionable monitoring and regulatory decision‑making.

Standards Content (Sample)

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01-junij-2017
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Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane
filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd
1:2016)
Wasserbeschaffenheit Zählung von Escherichia coli und coliformen Bakterien Teil 1:
Membranfiltrationsverfahren für Wässer mit niedriger Begleitflora (ISO 9308-1:2014/Amd
1:2016)
Qualité de l'eau - Dénombrement des Escherichia coli et des bactéries coliformes -
Partie 1: Méthode par filtration sur membrane pour les eaux à faible teneur en bactéries
(ISO 9308-1:2014/Amd 1:2016)
Ta slovenski standard je istoveten z: EN ISO 9308-1:2014/A1:2017
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 9308-1:2014/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2017
EUROPÄISCHE NORM
ICS 07.100.20
English Version
Water quality - Enumeration of Escherichia coli and
coliform bacteria - Part 1: Membrane filtration method for
waters with low bacterial background flora (ISO 9308-
1:2014/Amd 1:2016)
Qualité de l'eau - Dénombrement des Escherichia coli Wasserbeschaffenheit Zählung von Escherichia coli
et des bactéries coliformes - Partie 1: Méthode par und coliformen Bakterien Teil 1:
filtration sur membrane pour les eaux à faible teneur Membranfiltrationsverfahren für Wässer mit niedriger
en bactéries (ISO 9308-1:2014/Amd 1:2016) Begleitflora (ISO 9308-1:2014/Amd 1:2016)
This amendment A1 modifies the European Standard EN ISO 9308-1:2014; it was approved by CEN on 18 January 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 9308-1:2014/A1:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 9308-1:2014/A1:2017) has been prepared by Technical Committee ISO/TC 147
“Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat
of which is held by DIN.
This Amendment to the European Standard EN ISO 9308-1:2014 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by July 2017, and
conflicting national standards shall be withdrawn at the latest by July 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 9308-1:2014/Amd 1:2016 has been approved by CEN as EN ISO 9308-1:2
...