SIST EN 16934:2017

Automotive fuels and fat and oil derivates - Determination of steryl glycosides in fatty acid methyl esters (FAME) - Method by GC-MS with prior purification by SPE

This European Standard describes a procedure for the determination of steryl glycosides (SG) in pure fatty acid methyl ester (FAME, often referred to as biodiesel).
This method is suitable for the analysis of FAME prepared from vegetable oils. It is possible to quantify the SG content in homogenous samples as well as in samples where the SG started to precipitate.
WARNING — The use of this standard can involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this standard to take appropriate measures to ensure the safety and health of personnel prior to application of the standard, and fulfil statutory and regulatory requirements for this purpose.

Kraftstoffe und Erzeugnisse aus Fetten und Ölen - Bestimmung des Gehaltes an Sterylglycosiden in Fettsäure-Methylester (FAME) - Verfahren mittels GC-MS und vorausgehender Reinigung mit SPE

Diese Europäische Norm beschreibt ein Verfahren zur Bestimmung des Gehaltes von Sterylglycosiden (SG) in Fettsäure Methylester (FAME) im Bereich zwischen (20 und 38) mg/kg.
ANMERKUNG   Sterylglycoside (SG) befinden sich hauptsächlich in Pflanzenölen.
WARNUNG - Die Anwendung dieser Norm kann die Anwendung gefährlicher Stoffe, Arbeitsgänge und Geräte mit sich bringen. Diese Norm beansprucht nicht, alle damit verbundenen Sicherheits¬probleme zu behandeln. Es liegt in der Verantwortung des Anwenders dieser Norm, vor ihrer Anwendung geeignete Maßnahmen für die Sicherheit und den Gesundheitsschutz des Personals zu ergreifen, und dafür Sorge zu tragen, dass behördliche und gesetzliche Maßnahmen eingehalten werden.

Carburants pour automobiles et produits dérivés des corps gras - Détermination des stérols glucosides dans les esters méthyliques d’acides gras (EMAG) - Méthode par GC-MS avec purification préalable par SPE

La présente Norme européenne décrit une méthode de détermination de la teneur en stérols glucosides (SG) dans des esters méthyliques d'acides gras (EMAG) sur un intervalle compris entre 20 et 38 mg/kg.
NOTE   Les stérols glucosides (SG) sont principalement présents dans les huiles végétales.
AVERTISSEMENT — L'utilisation de la présente norme peut impliquer l'intervention de produits, d'opérations et d'équipements à caractère dangereux. La présente norme n'est pas censée aborder tous les problèmes de sécurité concernés par son usage. Il est de la responsabilité des utilisateurs de cette norme de prendre les mesures appropriées pour assurer la sécurité et préserver la santé du personnel avant son application, et pour répondre aux exigences réglementaires et statutaires à cette fin.

Goriva za motorna vozila ter maščobni in oljni derivati - Določevanje sterilglikozidov v metilnih estrih maščobnih kislin (FAME) - Metoda z GC-MS s predhodnim čiščenjem s SPE

Ta evropski standard opisuje postopek za določevanje sterilglikozidov (SG) v očiščenih metilnih estrih maščobnih kislin (FAME, ki se pogosto imenujejo biodizel).
Ta metoda je primerna za analizo metilnih estrov maščobnih kislin, pripravljenih iz rastlinskih olj. Vsebnost sterilglikozidov je mogoče kvantificirati v homogenih vzorcih in vzorcih, pri katerih se je sterilglikozid začel izločati.
OPOZORILO: Pri uporabi tega standarda so lahko prisotni nevarni materiali, postopki in oprema. Ta standard ne obravnava vseh varnostnih težav, ki se nanašajo na njegovo uporabo. Za sprejetje ustreznih ukrepov za zagotavljanje varnosti in zdravja osebja pred uporabo standarda ter izpolnjevanje zakonskih in regulativnih zahtev za ta namen so odgovorni uporabniki tega standarda.

General Information

• Status: Published
• Public Enquiry End Date: 03-Jan-2016
• Publication Date: 06-Sep-2017
• ICS: 75.160.20 - Liquid fuels
• Technical Committee: NAD - Petroleum products, lubricants and related products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 30-Aug-2017
• Due Date: 04-Nov-2017
• Completion Date: 07-Sep-2017
• Ref Project: EN 16934:2017 - Automotive fuels and fat and oil derivates - Determination of steryl glycosides in fatty acid methyl esters (FAME) - Method by GC-MS with prior purification by SPE

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 16934:2017
01-oktober-2017
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Automotive fuels and fat and oil derivates - Determination of steryl glycosides in fatty
acid methyl esters (FAME) - Method by GC-MS with prior purification by SPE
Kraftstoffe und Erzeugnisse aus Fetten und Ölen - Bestimmung des Gehaltes an
Sterylglycosiden in Fettsäure-Methylester (FAME) - Verfahren mittels GC-MS und
vorausgehender Reinigung mit SPE
Carburants pour automobiles et produits dérivés des corps gras - Détermination des
stérols glucosides dans les esters méthyliques d’acides gras (EMAG) - Méthode par GC-
MS avec purification préalable par SPE
Ta slovenski standard je istoveten z: EN 16934:2017
ICS:
75.160.20 7HNRþDJRULYD Liquid fuels
SIST EN 16934:2017 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 16934:2017

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SIST EN 16934:2017


EN 16934
EUROPEAN STANDARD

NORME EUROPÉENNE

August 2017
EUROPÄISCHE NORM
ICS 75.160.40
English Version

Automotive fuels and fat and oil derivates - Determination
of steryl glycosides in fatty acid methyl esters (FAME) -
Method by GC-MS with prior purification by SPE
Carburants pour automobiles et produits dérivés des Brennstoffe und Erzeugnisse aus Fetten und Ölen -
corps gras - Détermination des stérols glucosides dans Bestimmung des Gehaltes an Sterylglycosiden in
les esters méthyliques d'acides gras (EMAG) - Méthode Fettsäure-Methylester (FAME) - Verfahren mittels GC-
par GC-MS avec purification préalable par SPE MS und vorausgehender Reinigung mit SPE
This European Standard was approved by CEN on 12 June 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 16934:2017 E
worldwide for CEN national Members.

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SIST EN 16934:2017
EN 16934:2017 (E)
Contents Page
European foreword . 3
1 Scope . 4
2 Normative references . 4
3 Principle . 4
4 Reagents and materials . 4
5 Apparatus . 5
6 Sampling . 6
7 Procedure. 6
7.1 Sample purification . 6
7.2 Sample preparation and analysis . 7
7.3 Identification and integration peaks . 7
8 Calculation of results . 7
9 Expression of results . 8
10 Precision . 8
10.1 General . 8
10.2 Repeatability, r . 8
10.3 Reproducibility, R . 8
11 Test report . 8
Annex A (informative) Example chromatograms . 9
Bibliography . 12

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SIST EN 16934:2017
EN 16934:2017 (E)
European foreword
This document (EN 16934:2017) has been prepared by Technical Committee CEN/TC 19 “Gaseous and
liquid fuels, lubricants and related products of petroleum, synthetic and biological origin”, the
secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2018, and conflicting national standards
shall be withdrawn at the latest by February 2018.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
3

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SIST EN 16934:2017
EN 16934:2017 (E)
1 Scope
This European Standard describes a procedure for the determination of steryl glycosides (SG) content
in fatty acid methyl esters (FAME) in a range between 20 mg/kg and 38 mg/kg.
NOTE Steryl glycosides (SG) are mainly present in vegetable oils.
WARNING — The use of this standard can involve hazardous materials, operations and
equipment. This standard does not purport to address all of the safety problems associated with
its use. It is the responsibility of users of this standard to take appropriate measures to ensure
the safety and health of personnel prior to application of the standard, and fulfil statutory and
regulatory requirements for this purpose.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 3170, Petroleum liquids - Manual sampling (ISO 3170)
EN ISO 3171, Petroleum liquids - Automatic pipeline sampling (ISO 3171)
3 Principle
The steryl glycosides (SG) content of a FAME sample is determined by gas chromatography with mass
spectrometry detector (GC-MS) with cold on column (COC) or programmed temperature vaporization
(PTV) injection and measuring via selected ion monitoring (SIM).
Before injection into the GC instrument the sample is purified via solid phase extraction (SPE) and
derivatized with BSA+TMCS (4.2).
The SG content is quantified by using cholesteryl-β-D-glucopyranoside (4.3) as internal standard (IS).
4 Reagent
...