ASTM E260-96
(Practice)Standard Practice for Packed Column Gas Chromatography
Standard Practice for Packed Column Gas Chromatography
SCOPE
1.1 This practice is intended to serve as a general guide to the application of gas chromatography (GC) with packed columns for the separation and analysis of vaporizable or gaseous organic and inorganic mixtures and as a reference for the writing and reporting of GC methods.
Note 1--This practice excludes any form of gas chromatography associated with open tubular (capillary) columns.
1.2 This standard does not purport to address all the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8 and 9.1.3.
General Information
Relations
Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.
Designation: E 260 – 96
Standard Practice for
1
Packed Column Gas Chromatography
This standard is issued under the fixed designation E 260; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
3
1. Scope CGA P-12 Safe Handling of Cryogenic Liquids
3
HB-3 Handbook of Compressed Gases
1.1 This practice is intended to serve as a general guide to
the application of gas chromatography (GC) with packed
3. Terminology
columns for the separation and analysis of vaporizable or
3.1 Terms and relations are defined in Practice E 355 and
gaseous organic and inorganic mixtures and as a reference for
references therein.
the writing and reporting of GC methods.
NOTE 1—This practice excludes any form of gas chromatography 4. Summary of Practice
associated with open tubular (capillary) columns.
4.1 A block diagram of the basic apparatus needed for a gas
1.2 This standard does not purport to address all the safety
chromatographic system is as shown in Fig. 1. An inert,
concerns, if any, associated with its use. It is the responsibility pressure or flow-controlled carrier gas flowing at a measured
of the user of this standard to establish appropriate safety and
rate passes to the injection port or gas sample valve. A sample
health practices and determine the applicability of regulatory is introduced into the injection port, where it is vaporized, or if
limitations prior to use. Specific hazard statements are given in
gaseous, into a gas sample valve, and then swept into and
Section 8 and 9.1.3. through the column by the carrier gas. Passage through the
column separates the sample into its components. The effluent
2. Referenced Documents
from the column passes to a detector where the response of
2.1 ASTM Standards:
sample components is measured as they emerge from the
E 355 Practice for Gas Chromatography Terms and Rela-
column. The detector electrical output is relative to the
2
tionships
concentration of each resolved component and is transmitted to
E 516 Practice for Testing Thermal Conductivity Detectors
a recorder, or electronic data processing system, or both, to
2
Used in Gas Chromatography
produce a record of the separation, or chromatogram, from
E 594 Practice for Testing Flame Ionization Detectors Used
which detailed analysis can be obtained. The detector effluent
2
in Gas Chromatography
must be vented to a hood if the effluent contains toxic
E 697 Practice for Use of Electron Capture Detectors in Gas
substances.
2
Chromatography
4.2 Gas chromatography is essentially a physical separation
E 840 Practice for Using Flame Photometric Detectors in
technique. The separation is obtained when the sample mixture
2
Gas Chromatography
in the vapor phase passes through a column containing a
E 1140 Practice for Testing Nitrogen/Phosphorus Thermi-
stationary phase possessing special adsorptive properties. The
2
onic Ionization Detectors for Use in Gas Chromatography
degree of separation depends upon the differences in the
2.2 CGA Publications:
distribution of volatile compounds, organic or inorganic, be-
CGA P-1 Safe Handling of Compressed Gases in Contain-
tween a gaseous mobile phase and a selected stationary phase
3
ers
that is contained in a tube or GC column. In gas-liquid
CGA G-5.4 Standard for Hydrogen Piping Systems at Con-
chromatography (GLC), the stationary phase is a nonvolatile
3
sumer Locations
liquid or gum coated as a thin film on a finely-divided, inert
3
CGA P-9 The Inert Gases: Argon, Nitrogen and Helium
support of a relatively large surface area, and the distribution is
CGA V-7 Standard Method of Determining Cylinder Valve
based on partition. The liquid phase should not react with, and
3
Outlet Connections for Industrial Gas Mixtures
should have different partition coefficients for, the various
components in the sample. In gas-solid chromatography
1
(GSC), the stationary phase is a finely divided solid adsorbent
This practice is under the jurisdiction of ASTM Committee E13 on Molecular
Spectroscopy and is the direct responsibility of Subcommittee E13.19 on Chroma-
(see 4.4).
tography.
4.2.1 After separation in the analytical column, the compo-
Current edition approved April 10, 1996. Published June 1996. Originally
nents are detected, and the detector signal is related to the
published as E 260 – 65 T. Last previous edition E 260 – 96.
2
Annual Book of ASTM Standards, Vol 14.02. concentration of the volatile components. Tentative identifica-
3
Available from Compressed Gas Association, Inc., 1725 Jefferson Davis
tions can be made by comparison with the retention times of
Highway, Arlington, VA 22202-4100.
Copyr
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.