EN 17719:2024
(Main)Plant biostimulants - Determination of the anaerobic plate count
Plant biostimulants - Determination of the anaerobic plate count
This document specifies a horizontal method for the enumeration of microorganisms that are able to grow and form colonies in a solid medium after anaerobic incubation at 30 °C.
The method applies to microbial plant biostimulants, except those composed of aerobic bacteria.
This method does not apply to the microbiological monitoring of the environment in which microbial plant biostimulants are manufactured.
No information about potential human pathogens can be inferred from anaerobic plate counts.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of the following component EU fertilizing products categories: Fertilizers, Liming Materials, Soil Improvers, Growing Media, Plant Biostimulants and where the following category Plant Biostimulants is the highest percentage in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not the highest percentage in the blend, the European Standard for the highest percentage of the blend applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the component EU fertilising products used for the blend have identical formulations , the user decides which standard to apply.
Pflanzen-Biostimulanzien - Bestimmung der anaeroben Keimzahl
Dieses Dokument legt ein horizontales Verfahren zur Zählung von Mikroorganismen fest, die nach anaerober Bebrütung bei 30 °C in einem festen Medium wachsen und Kolonien bilden können.
Das Verfahren gilt für mikrobielle Pflanzen-Biostimulanzien, ausgenommen jene, die aus aeroben Bakterien bestehen.
Dieses Verfahren ist nicht anzuwenden für die mikrobiologische Überwachung der Umgebung, in der die mikrobiellen Pflanzen-Biostimulanzien hergestellt werden.
Es können keine Informationen über potentielle humanpathogene Keime aus der Zählung von Anaerobier¬platten abgeleitet werden.
Dieses Dokument ist für die Mischungen von Düngeprodukten anwendbar, in denen eine Mischung aus mindestens zwei der folgenden Kategorien von Komponenten-EU Düngeprodukten besteht: Düngemittel, Kalkdünger, Bodenverbesserungsmittel, Kultursubstrate, Pflanzen-Biostimulanzien und wenn die folgende Kategorie Pflanzen-Biostimulanzien prozentual den höchsten Massen- oder Volumenanteil in der Mischung bzw., bei flüssiger Form, den höchsten Trockenmasseanteil aufweist. Wenn Pflanzen-Biostimulanzien nicht den höchsten prozentualen Anteil in der Mischung ausmachen, ist die für den höchsten prozentualen Anteil geltende Europäische Norm anwendbar. Falls eine Düngemittelmischung aus Komponenten gleicher Menge besteht oder falls die für die Mischung verwendeten Komponenten-EU Düngeprodukte identische Formulierungen haben, entscheidet der Anwender, welche Norm angewendet wird.
Biostimulants des végétaux - Détermination du dénombrement sur plaque des germes anaérobies
Le présent document spécifie une méthode horizontale pour le dénombrement des microorganismes qui sont capables de croître et de former des colonies dans un milieu solide après une incubation anaérobie à 30 °C.
La méthode s’applique aux biostimulants microbiens des végétaux, excepté ceux composés de bactéries aérobies.
La présente méthode ne s’applique pas à la surveillance microbiologique de l’environnement dans lequel les biostimulants microbiens des végétaux sont fabriqués.
Aucune information sur les pathogènes humains potentiels ne peut être déduite des dénombrements sur plaque des germes anaérobies.
Le présent document est applicable aux mélanges de fertilisants constitués d’au moins deux composants appartenant aux catégories de fertilisants UE suivantes : engrais, amendements minéraux basiques, amendements du sol, supports de culture, biostimulants des végétaux et, dans lesquels la catégorie Biostimulants des végétaux représente le pourcentage le plus élevé du mélange en masse ou en volume, ou dans le cas d’un liquide, en masse sèche. Si les biostimulants des végétaux ne représentent pas le pourcentage le plus élevé du mélange, la Norme européenne relative au composant représentant le pourcentage le plus élevé du mélange s’applique. Dans le cas où un mélange de fertilisants contient des composants en quantité égale ou dans le cas où les composants des fertilisants UE utilisés pour le mélange ont des formulations identiques, l’utilisateur décide quelle norme appliquer.
Rastlinski biostimulanti - Ugotavljanje števila anaerobnih mikroorganizmov na mikrotitrskih ploščah
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN 17719:2023
01-maj-2023
Rastlinski biostimulanti - Ugotavljanje števila na anaerobnih mikrotitrskih ploščah
Plant biostimulants - Determination of the anaerobic plate count
Pflanzen-Biostimulanzien - Bestimmung der anaeroben Keimzahl
Biostimulants des végétaux - Détermination du dénombrement sur plaque des germes
anaérobies
Ta slovenski standard je istoveten z: prEN 17719
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17719:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN 17719:2023
oSIST prEN 17719:2023
DRAFT
EUROPEAN STANDARD
prEN 17719
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2023
ICS 65.080 Will supersede CEN/TS 17719:2022
English Version
Plant biostimulants - Determination of the anaerobic plate
count
Biostimulants des végétaux - Détermination du Pflanzen-Biostimulanzien - Bestimmung der anaeroben
dénombrement sur plaque des germes anaérobies Keimzahl
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17719:2023 E
worldwide for CEN national Members.
oSIST prEN 17719:2023
prEN 17719:2023 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 7
2 Normative references . 7
3 Terms and definitions . 7
4 Principle . 8
4.1 General. 8
4.2 Brief description . 8
5 Culture media and reagents . 8
6 Equipment and consumables . 8
7 Preparation of test sample . 9
7.1 General. 9
7.2 Liquid – water-based formulations . 9
7.3 Liquid – oil-based (emulsifiable concentrate - EC) formulations . 9
7.4 Solid – wettable powder (WP) formulations . 9
7.5 Solid – water dispersible granules (WDG) formulations . 9
7.6 Solid – pellets, granules, microgranules (slow release) formulations . 10
7.7 Solid substrates . 10
8 Procedure . 10
8.1 Test portion, initial suspension and dilutions . 10
8.2 Inoculation and incubation . 10
8.3 Critical control point. 10
9 Calculation . 10
10 Expression of results . 11
11 Performance characteristics of the method . 11
11.1 Interlaboratory studies . 11
11.2 Sensitivity . 11
11.3 Specificity . 11
12 Test report . 11
13 Quality assurance . 11
Annex A (informative) Guidelines for calculating and reporting the Anaerobic Plate Counts
(ANPC) . 12
A.1 General. 12
A.2 Example . 12
Annex B (normative) Composition and preparation of culture media and reagents . 13
B.1 Anaerobe Agar (ANA) . 13
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prEN 17719:2023 (E)
Annex C (informative) Results of the interlaboratory study for the determination of the
anaerobic plate count . 15
C.1 Results of the interlaboratory study . 15
C.2 Statistical analysis . 15
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 17
Bibliography . 18
oSIST prEN 17719:2023
prEN 17719:2023 (E)
European foreword
This document (prEN 17719:2023) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the CEN enquiry.
This document will supersede CEN/TS 17719:2022.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association, and supports essential requirements of EU
Directive(s) / Regulation(s).
For relationship with EU Directive(s) / Regulation(s), see informative Annex ZA, which is an integral part
of this document.
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prEN 17719:2023 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 “Plant Biostimulants”. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 laying down rules on the making available on the market of
EU fertilising products (“FPR” or “Fertilising Products Regulation”). This standardization request,
presented as SR M/564 and M/564 Amd1, also contributes to the Communication on “Innovating for
Sustainable Growth: A Bio economy for Europe”. Working Group 5 “Labelling and denominations”, was
created to develop a work program as part of this standardization request.
Technical committee CEN/TC 455 “Plant Biostimulants” was established to carry out the work program
that will prepare a series of standards. The interest in biostimulants has increased significantly in Europe
as a valuable tool to use in agriculture. Standardization was identified as having an important role in
order to promote the use of biostimulants. The work of CEN/TC 455 seeks to improve the reliability of
the supply chain, thereby improving the confidence of farmers, industry, and consumers in biostimulants,
and will promote and support commercialisation of the European biostimulant industry.
Biostimulants used in agriculture can be applied in multiple ways: on soil, on plants, as seed treatment,
etc. A microbial plant biostimulant consists of a microorganism or a consortium of microorganisms, as
referred to in Component Material Category 7 of Annex II of the EU Fertilising Products Regulation
2019/1009 [1].
The method is applicable to microbial plant biostimulants except those composed of aerobic bacterium
to verify that the concentration of anaerobes does not exceed the respective limits described in the EU
Fertilisers Regulation.
Table 1 summarizes many of the agro-ecological principles and the role played by biostimulants.
Table 1 — Agro-ecological principles and the role played by biostimulants
Increase biodiversity
By improving soil microorganism quality/quantity
Reinforce biological regulation and interactions
By reinforcing plant-microorganism interactions
— symbiotic exchanges i.e. mycorrhize
— symbiotic exchanges i.e. rhizobiaciae/fava
— secretions mimicking plant hormones (i.e. trichoderma)
By regulating plant physiological processes
— e.g. growth, metabolism, plant development…
Improve biogeochemical cycles
— improve absorption of nutritional elements
— improve bioavailability of nutritional elements in the soil
— stimulate degradation of organic matter
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prEN 17719:2023 (E)
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
oSIST prEN 17719:2023
prEN 17719:2023 (E)
1 Scope
This document provides a horizontal method for enumeration of microorganisms that are able to grow
and form colonies in a solid medium after anaerobic incubation at 30 °C.
The method is applicable to microbial plant biostimulants, except those composed of aerobic bacteria, for
verifying that the concentration of anaerobes does not exceed the respective limits outlined in the EU
Fertilizers Regulation [1].
This method does not apply to the microbiological monitoring of the environment in which microbial
plant biostimulants are manufactured.
No information about potential human pathogens can be inferred from anaerobic plate counts.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17708:— , Plant biostimulants — Preparation of sample for microbial analysis
EN 17724:— , Plant biostimulants — Terminology
EN ISO 7218:2007 , Microbiology of food and animal feeding stuffs — General requirements and guidance
for microbiological examinations (ISO 7218:2007)
EN ISO 11133:2014 , Microbiology of food, animal feed and water — Preparation, productio
...
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