EN ISO 11133:2014
(Main)Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media (ISO 11133:2014)
Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media (ISO 11133:2014)
ISO 11133:2014 defines terms related to quality assurance of culture media and specifies the requirements for the preparation of culture media intended for the microbiological analysis of food, animal feed, and samples from the food or feed production environment as well as all kinds of water intended for consumption or used in food production. These requirements are applicable to all categories of culture media prepared for use in laboratories performing microbiological analyses.
ISO 11133:2014 also sets criteria and describes methods for the performance testing of culture media. It applies to producers such as:
commercial bodies producing and/or distributing ready-to-use or semi-finished reconstituted or dehydrated media;
non-commercial bodies supplying media to third parties;
microbiological laboratories preparing culture media for their own use.
Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser - Vorbereitung, Herstellung, Lagerung und Leistungsprüfung von Nährmedien (ISO 11133:2014)
Diese Internationale Norm enthält die allgemeine Terminologie hinsichtlich der Qualitätssicherung und legt die Anforderungen an die Zubereitung von Nährmedien fest, die für mikrobiologische Untersuchungen von Produkten zu verwenden sind, die für den menschlichen Verzehr oder als Futtermittel vorgesehen sind, von Umgebungsproben aus der Lebensmittelproduktion sowie aller Arten von Wasser.
Diese Anforderungen gelten für folgende vier Arten von in Laboratorien verwendeten Nährmedien; wobei das Laboratorium für die mikrobiologischen Untersuchungen Nährmedien herstellt und/oder verwendet:
- gewerblich hergestellte Fertigmedien;
- gewerblich hergestellte Medien, die erneut zu verflüssigen, zu supplementieren und abzufüllen sind;
- aus handelsüblichen Trockenformulierungen hergestellte Medien;
- aus einzelnen Grundsubstanzen hergestellte Medien.
Diese Internationale Norm legt außerdem Kriterien fest und beschreibt Verfahren für die Leistungsprüfung von Nährmedien. Diese Internationale Norm gilt für:
- kommerzielle Einrichtungen, die gebrauchsfertig zubereitete Medien, Halbfertigmedien oder Trockenmedien herstellen und/oder vertreiben;
- nichtkommerzielle Einrichtungen, die Medien an Dritte liefern;
- mikrobiologische Laboratorien, die Nährmedien für ihre eigene Verwendung herstellen.
Microbiologie des aliments, des aliments pour animaux et de l'eau - Préparation, production, stockage et essais de performance des milieux de culture (ISO 11133:2014)
L'ISO 11133:2014 définit les termes relatifs à l'assurance qualité des milieux de culture et spécifie les exigences relatives à la préparation des milieux de culture destinés à être appliqués pour l'analyse microbiologique des aliments, des aliments pour animaux et des d'échantillons de la production d'aliments et d'aliments pour animaux ainsi que de tous les types d'eau destinés à la consommation ou utilisés dans la production alimentaire. Ces exigences sont applicables à toutes catégories de milieux de culture préparés pour être utilisés dans les laboratoires qui réalisent des analyses microbiologiques.
L'ISO 11133:2014 définit également des critères et décrit des méthodes pour les essais de performance des milieux de culture. Elle s'applique aux producteurs tels que:
les entités commerciales qui produisent et/ou distribuent des milieux prêts à l'emploi, semi-finis reconstitués ou déshydratés;
les entités non commerciales qui fournissent des milieux à des tiers;
les laboratoires de microbiologie qui préparent des milieux de culture pour leur propre usage.
Mikrobiologija živil, krme in vode - Priprava, izdelava, skladiščenje in preskušanje lastnosti gojišč (ISO 11133:2014)
Standard EN ISO 11133 določa izraze, povezane z zagotavljanjem kakovosti gojišč, in opredeljuje zahteve za pripravo gojišč, namenjenih mikrobiološki analizi živil, krme in vzorcev iz proizvodnega okolja živil ali krme ter tudi vseh vrst vode, namenjene pitju ali uporabi pri proizvodnji živil. Te zahteve se uporabljajo za vse kategorije gojišč, pripravljenih za uporabo v laboratorijih, ki izvajajo mikrobiološke analize. Ta mednarodni standard določa tudi merila in opisuje metode za preskušanje lastnosti gojišč. Ta mednarodni standard se uporablja za proizvajalce, kot so: – komercialni organi, ki proizvajajo in/ali distribuirajo na uporabo pripravljena ali polpripravljena rekonstituirana ali dehidrirana gojišča; – nekomercialni organi, ki gojišča dobavljajo tretjim strankam; – mikrobiološki laboratoriji, ki pripravljajo gojišča za lastno uporabo.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-oktober-2014
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SIST-TS CEN ISO/TS 11133-1:2009
SIST-TS CEN ISO/TS 11133-2:2004
SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
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Microbiology of food, animal feed and water - Preparation, production, storage and
performance testing of culture media (ISO 11133:2014)
Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser - Vorbereitung, Herstellung,
Lagerung und Leistungsprüfung von Nährmedien (ISO 11133:2014)
Microbiologie des aliments, des aliments pour animaux et de l'eau - Préparation,
production, stockage et essais de performance des milieux de culture (ISO 11133:2014)
Ta slovenski standard je istoveten z: EN ISO 11133:2014
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 11133
NORME EUROPÉENNE
EUROPÄISCHE NORM
May 2014
ICS 07.100.30 Supersedes CEN ISO/TS 11133-1:2009, CEN ISO/TS
11133-2:2003
English Version
Microbiology of food, animal feed and water - Preparation,
production, storage and performance testing of culture media
(ISO 11133:2014)
Microbiologie des aliments, des aliments pour animaux et Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser
de l'eau - Préparation, production, stockage et essais de - Vorbereitung, Herstellung, Lagerung und
performance des milieux de culture (ISO 11133:2014) Leistungsprüfung von Nährmedien (ISO 11133:2014)
This European Standard was approved by CEN on 20 March 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11133:2014 E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (EN ISO 11133:2014) has been prepared by Technical Committee ISO/TC 34 “Food products”
in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by November 2014, and conflicting national standards shall be withdrawn
at the latest by November 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN ISO/TS 11133-2:2003, CEN ISO/TS 11133-1:2009.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11133:2014 has been approved by CEN as EN ISO 11133:2014 without any modification.
INTERNATIONAL ISO
STANDARD 11133
First edition
2014-05-15
Microbiology of food, animal feed and
water — Preparation, production,
storage and performance testing of
culture media
Microbiologie des aliments, des aliments pour animaux et de l’eau —
Préparation, production, stockage et essais de performance des
milieux de culture
Reference number
ISO 11133:2014(E)
©
ISO 2014
ISO 11133:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2014 – All rights reserved
ISO 11133:2014(E)
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
3.1 General terms and definitions . 2
3.2 Terminology of performance testing . 2
3.3 Terminology of culture media . 3
3.4 Terminology for test microorganisms . 6
4 Quality assurance management . 7
4.1 Documentation . 7
4.2 Storage . 8
4.3 Laboratory preparation of media . 8
4.4 Storage and shelf-life of prepared media .11
4.5 Preparation for use .12
4.6 Incubation of solid media in Petri dishes .14
4.7 Disposal of media .14
5 Test organisms for performance testing.14
5.1 General .14
5.2 Selection of test organisms .14
5.3 Preservation and maintenance of test organisms .15
5.4 Microorganisms for performance testing .16
6 Quality control and performance testing of culture media .19
6.1 General requirements .19
6.2 Physical and chemical quality control .19
6.3 Microbiological quality control .19
6.4 General requirements for microbiological performance testing .20
6.5 Performance evaluation and interpretation of results .21
6.6 Confirmation media and reagents .22
7 Methods for performance testing of solid culture media .22
7.1 General .22
7.2 Methods for quantitative tests.22
7.3 Testing of culture media used for membrane filtration .24
7.4 Methods for qualitative tests .24
8 Methods for performance testing of liquid culture media.25
8.1 General .25
8.2 Quantitative tube method for performance testing of liquid enrichment media (dilution to
extinction method) .25
8.3 Qualitative tube method for performance testing of selective liquid media .26
8.4 Qualitative single tube method (turbidity) for performance testing of liquid media .27
9 Methods for performance testing of diluents and transport media .28
9.1 General .28
9.2 Method for testing diluents .28
9.3 Method for testing transport media .29
10 Documentation of test results .30
10.1 Information provided by the manufacturer .30
10.2 Traceability .30
Annex A (informative) Designation of the components of culture media in Inter
...
SLOVENSKI STANDARD
01-oktober-2014
Nadomešča:
SIST ISO 9998:1998
SIST-TS CEN ISO/TS 11133-1:2009
SIST-TS CEN ISO/TS 11133-2:2004
SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
Mikrobiologija živil, krme in vode - Priprava, izdelava, skladiščenje in preskušanje
lastnosti gojišč (ISO 11133:2014)
Microbiology of food, animal feed and water - Preparation, production, storage and
performance testing of culture media (ISO 11133:2014)
Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser - Vorbereitung, Herstellung,
Lagerung und Leistungsprüfung von Nährmedien (ISO 11133:2014)
Microbiologie des aliments, des aliments pour animaux et de l'eau - Préparation,
production, stockage et essais de performance des milieux de culture (ISO 11133:2014)
Ta slovenski standard je istoveten z: EN ISO 11133:2014
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 11133
NORME EUROPÉENNE
EUROPÄISCHE NORM
May 2014
ICS 07.100.30 Supersedes CEN ISO/TS 11133-1:2009, CEN ISO/TS
11133-2:2003
English Version
Microbiology of food, animal feed and water - Preparation,
production, storage and performance testing of culture media
(ISO 11133:2014, Corrected version 2014-11-01)
Microbiologie des aliments, des aliments pour animaux et Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser
de l'eau - Préparation, production, stockage et essais de - Vorbereitung, Herstellung, Lagerung und
performance des milieux de culture (ISO 11133:2014, Leistungsprüfung von Nährmedien (ISO 11133:2014,
Version corrigée 2014-11-01) korrigierte Fassung 2014-11-01)
This European Standard was approved by CEN on 20 March 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11133:2014 E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (EN ISO 11133:2014) has been prepared by Technical Committee ISO/TC 34 “Food products”
in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by November 2014, and conflicting national standards shall be withdrawn
at the latest by November 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN ISO/TS 11133-2:2003, CEN ISO/TS 11133-1:2009.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11133:2014, Corrected version 2014-11-01 has been approved by CEN as
INTERNATIONAL ISO
STANDARD 11133
First edition
2014-05-15
Microbiology of food, animal feed and
water — Preparation, production,
storage and performance testing of
culture media
Microbiologie des aliments, des aliments pour animaux et de l’eau —
Préparation, production, stockage et essais de performance des
milieux de culture
Reference number
ISO 11133:2014(E)
©
ISO 2014
ISO 11133:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2014 – All rights reserved
ISO 11133:2014(E)
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
3.1 General terms and definitions . 2
3.2 Terminology of performance testing . 2
3.3 Terminology of culture media . 3
3.4 Terminology for test microorganisms . 6
4 Quality assurance management . 7
4.1 Documentation . 7
4.2 Storage . 8
4.3 Laboratory preparation of media . 8
4.4 Storage and shelf-life of prepared media .11
4.5 Preparation for use .12
4.6 Incubation of solid media in Petri dishes .14
4.7 Disposal of media .14
5 Test organisms for performance testing.14
5.1 General .14
5.2 Selection of test organisms .14
5.3 Preservation and maintenance of test organisms .15
5.4 Microorganisms for performance testing .16
6 Quality control and performance testing of culture media .19
6.1 General requirements .19
6.2 Physical and chemical quality control .19
6.3 Microbiological quality control .19
6.4 General requirements for microbiological performance testing .20
6.5 Performance evaluation and interpretation of results .21
6.6 Confirmation media and reagents .22
7 Methods for performance testing of solid culture media .22
7.1 General .22
7.2 Methods for quantitative tests.22
7.3 Testing of culture media used for membrane filtration .24
7.4 Methods for qualitative tests .24
8 Methods for performance testing of liquid culture media.25
8.1 General .25
8.2 Quantitative tube method for performance testing of liquid enrichment media (dilution to
extinction method) .25
8.3 Qualitative tube method for performance testing of selective liquid media .26
8.4 Qualitative single tube method (turbidity) for performance testing of liquid media .27
9 Methods for performance testing of diluents and transport media .28
9.1 General .28
9.2 Method for testing diluents .28
9.3 Method for testing transport media .29
10 Documentation of test results .30
10.1 Information provided by the manufacturer .30
10.2 Traceability .
...
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