EN ISO 11133:2014/A1:2018
(Amendment)Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media - Amendment 1 (ISO 11133:2014/Amd 1:2018)
Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media - Amendment 1 (ISO 11133:2014/Amd 1:2018)
Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser - Vorbereitung, Herstellung, Lagerung und Leistungsprüfung von Nährmedien (ISO 11133:2014/Amd 1:2018)
Den letzten Absatz durch folgenden ersetzen:
Dieses Dokument legt außerdem Kriterien fest und beschreibt Verfahren für die Leistungsprüfung von Nährmedien. Dieses Dokument gilt für Endanwender gebrauchsfertiger Nährmedien und für herstellende Einrichtungen wie
— kommerzielle Einrichtungen, die gebrauchsfertig zubereitete Medien, Halbfertigmedien oder Trockenmedien herstellen und/oder vertreiben,
— nichtkommerzielle Einrichtungen, die Medien an Dritte liefern und
— mikrobiologische Laboratorien, die Nährmedien für ihre eigene Verwendung herstellen.
Microbiologie des aliments, des aliments pour animaux et de l'eau - Préparation, production, stockage et essais de performance des milieux de culture - Amendement 1 (ISO 11133:2014/Amd 1:2018)
Mikrobiologija živil, krme in vode - Priprava, izdelava, skladiščenje in preskušanje lastnosti gojišč - Dopolnilo A1 (ISO 11133:2014/Amd 1:2018)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-maj-2018
0LNURELRORJLMDåLYLONUPHLQYRGH3ULSUDYDL]GHODYDVNODGLãþHQMHLQSUHVNXãDQMH
ODVWQRVWLJRMLãþ'RSROQLOR$ ,62 $PG
Microbiology of food, animal feed and water - Preparation, production, storage and
performance testing of culture media - Amendment 1 (ISO 11133:2014/Amd 1:2018)
Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser - Vorbereitung, Herstellung,
Lagerung und Leistungsprüfung von Nährmedien - Änderung 1 (ISO 11133:2014/Amd
1:2018)
Microbiologie des aliments, des aliments pour animaux et de l'eau - Préparation,
production, stockage et essais de performance des milieux de culture - Amendement 1
(ISO 11133:2014/Amd 1:2018)
Ta slovenski standard je istoveten z: EN ISO 11133:2014/A1:2018
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 11133:2014/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2018
EUROPÄISCHE NORM
ICS 07.100.20; 07.100.30
English Version
Microbiology of food, animal feed and water - Preparation,
production, storage and performance testing of culture
media - Amendment 1 (ISO 11133:2014/Amd 1:2018)
Microbiologie des aliments, des aliments pour animaux Mikrobiologie von Lebensmitteln, Futtermitteln und
et de l'eau - Préparation, production, stockage et essais Wasser - Vorbereitung, Herstellung, Lagerung und
de performance des milieux de culture - Amendement Leistungsprüfung von Nährmedien - Änderung 1 (ISO
1 (ISO 11133:2014/Amd 1:2018) 11133:2014/Amd 1:2018)
This amendment A1 modifies the European Standard EN ISO 11133:2014; it was approved by CEN on 28 December 2017.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11133:2014/A1:2018 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 11133:2014/A1:2018) has been prepared by Technical Committee ISO/TC 34
“Food products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal
methods” the secretariat of which is held by DIN.
This Amendment to the European Standard EN ISO 11133:2014 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by September 2018,
and conflicting national standards shall be withdrawn at the latest by September 2018.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11133:2014/A1:2018 has been approved by CEN as EN ISO 11133:2014/A1:2018
without any modification.
INTERNATIONAL ISO
STANDARD 11133
First edition
2014-05-15
AMENDMENT 1
2018-02
Microbiology of food, animal feed and
water — Preparation, production,
storage and performance testing of
culture media
AMENDMENT 1
Microbiologie des aliments, des aliments pour animaux et de l'eau —
Préparation, production, stockage et essais de performance des
milieux de culture
AMENDEMENT 1
Reference number
ISO 11133:2014/Amd.1:2018(E)
©
ISO 2018
ISO 11133:2014/Amd.1:2018(E)
© ISO 2018
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
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copyright@iso.org
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Published in Switzerland
ii © ISO 2018 – All rights reserved
ISO 11133:2014/Amd.1:2018(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www .iso .org/ iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology, in collaboration with Technical Committee ISO/TC 147 Water quality, Subcommittee SC 4,
Microbiological methods.
ISO 11133:2014/Amd.1:2018(E)
Microbiology of food, animal feed and water —
Preparation, production, storage and performance testing
of culture media
AMENDMENT 1
Introduction
Add the following text as the last paragraph:
When specific standards are revised and new standards developed, they will include a paragraph for
performance testing of the culture media used in the standard.
Scope
Replace the last paragraph with the following:
This document also sets criteria and describes methods for the performance testing of culture media.
This document is applicable to end-users of ready-to-use media and to producers such as
— commercial bodies producing and/or distributing ready-to-use or semi-finished reconstituted or
dehydrated media,
— non-commercial bodies supplying media to third parties, and
— microbiological laboratories preparing culture media for their own use.
3.2.6, electivity of culture medium
Replace the definition with the following:
demonstration, under defined conditions, that non-target organisms, if able to grow on the medium, do
not show the same visual characteristics as target microorganisms
4.3.1 General
Add the following text as the third paragraph:
When a formula indicates an ingredient in hydrated form (e.g. Na HPO ·12H O for BPW in ISO 6887-1)
2 4 2
it can be replaced by an anhydrous or hydrated ingredient with a different number of water molecules,
as long as the final quantity of the ingredient takes account of this difference by calculation of the
molar mass.
ISO 11133:2014/Amd.1:2018(E)
4.3.8.1 General
Replace the last sentence with the following:
In all cases, make reference to the appropriate International Standard or the manufacturer’s
instructions.
5.4.2.5.1.1 Quantitative testing
Replace the first two paragraphs with the following:
For the quantitative enumeration test, a level of approximately 100 cfu is necessary to achieve sufficient
precision (see Table 1). This can necessitate the use of more than one plate.
A practicable range of 80 cfu to 120 cfu per plate with a minimum number of 50 cfu per plate should
be used. The use of more than one plate will increase the precision. For filters, the same number of
cfu is needed using one or more filters. Table 1 shows the 95 % confidence intervals associated with
colony counts.
5.4.2.5.1.2 Qualitative testing
Replace the part of the sentence introducing the list with the following:
The volume of suspension used for testing should contain
5.4.2.5.2 Inoculum level for selectivity testing
Replace the sentence with the following:
For selectivity testing of culture media, a suspension of the non-target microorganism containing at
least 10 cfu is inoculated on to the plate or into the tube of medium.
5.4.2.5.3 Inoculum level for specificity testing
Replace the sentence with the following:
For qualitative tests of plate media, for specificity an inoculum level of at least 10 cfu is needed.
7.3 Testing of culture media used for membrane filtration
Add the following text as the last paragraph:
When testing with membrane filters, if the criteria in Table F1 are not achieved, the laboratory should
assess the discrepancies between the results.
8.3.2 Procedure
Replace the fourth list item with the following:
— Inoculation of non-target microorganisms: Inoculate one tube of test broth per microorganism
with an inoculum containing a higher number (at least 10 cfu) and mix.
2 © ISO 2018 – All rights reserved
ISO 11133:2014/Amd.1:2018(E)
Replace the seventh, eighth and ninth list items with the following:
— Remove one loopful (10 μl) from the tube containing the target organism and streak on a plate of the
relevant selective medium (e.g. XLD).
— Remove one loop (10 µl) from the culture of non-target microorganism and streak on a plate of a
non-selective medium (e.g. TSA).
— If a mixed culture of target and non-target organisms has been used, remove one loop (10 µl) and
streak on a plate of the specific medium for the target microorganism (e.g. XLD).
Replace the last paragraph with the following:
If a larger volume of medium is used (e.g. 225 ml), the user may choose to adjust the volume of inoculum
proportionately, but without changing the total number of organisms inoculated.
8.3.3 Calculation and interpretation of results
Replace the last paragraph with the following:
Selectivity of the liquid test broth is satisfactory if either no growth, or less than 10 cfu or less than 100
cfu of non-target microorganisms occurs on the non-selective agar plate, as specified in Annexes E and
F or the specific International Standard.
8.4.2.2 Confirmation media
Replace the first list item with the following:
— For performance testing of liquid confirmation media, inoculate the medium under test with ≥ 10
cfu of the target organism (for example, by using a working culture suspension containing more
than 10 cfu/ml and a 10 μl loop).
After 8.4.3
Add the following text as a new subclause:
8.5 Multipurpose liquid media
For multipurpose liquid media such as BPW, perform a qualitative pre-enrichment test as a minimum,
using a pathogenic organism appropriate to the laboratory’s range of test methods, e.g. Salmonella.
Commercial and non-commercial suppliers of multipurpose liquid media are also expected to test
these multipurpose liquid media as diluents for enumerations of microorganisms to further ensure the
quality of the culture media they supply.
If a new or revised standard uses a multipurpose medium as part of the isolation procedure for a target
organism not currently included in this document (
...
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