EN ISO 22119:2011

Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011)

ISO 22119:2010 defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). ISO 22119:2010 specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR.
The minimum requirements laid down in ISO 22119:2010 provide the basis for comparable and reproducible results within individual and between different laboratories.
ISO 22119:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.

Mikrobiologie von Lebensmitteln und Futtermitteln - Real-time-Polymerase-Kettenreaktion (PCR) zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Allgemeine Anforderungen und Begriffe (ISO 22119:2011)

Die vorliegende Norm gilt für den Nachweis von pathogenen Mikroorganismen in Lebensmitteln und in aus Lebensmitteln isolierten Bakterienisolaten unter Anwendung der Polymerase-Kettenreaktion (PCR). Die Norm beschreibt die Anforderungen an die Amplifikation (Vervielfältigung) und den Nachweis von Nukleinsäure¬sequenzen (DNS oder RNS nach Umschreibung durch reverse Transkription) durch Real-Time-PCR.
Die in dieser Norm festgelegten Mindestanforderungen liefern die Grundlage für vergleichbare und reprodu¬zierbare Ergebnisse in einzelnen Laboratorien oder zwischen verschiedenen Laboratorien.
ANMERKUNG 1   Die Norm kann auch für andere Zwecke im Lebensmittelsektor (z. B. zum Nachweis von über Nahrungsmittel übertragenen pathogenen Mikroorganismen in Umgebungsproben oder für Tierfuttermittel angewendet werden.
ANMERKUNG 2   Auf Grund des schnellen Fortschritts auf diesem Gebiet entsprechen die angegebenen Beispiele den zum Zeitpunkt der Erarbeitung dieser Norm häufigsten Anwendungen.

Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) en temps réel pour la détection des micro-organismes pathogènes dans les aliments - Exigences générales et définitions (ISO 22119:2011)

L'ISO 22119:2010 définit les termes relatifs à la détection de micro-organismes pathogènes présents dans les aliments et les extraits obtenus à partir de ces aliments, en faisant appel à la réaction de polymérisation en chaîne (PCR). L'ISO 22119:2010 spécifie également les exigences relatives à l'amplification et à la détection des séquences d'acide nucléique (ADN ou ARN après transcription inverse) par une PCR en temps réel.
Les exigences minimales spécifiées dans l'ISO 22119:2010 servent de base pour l'obtention de résultats comparables et reproductibles au sein d'un laboratoire et dans des laboratoires différents.
L'ISO 22119:2010 peut également être utilisée, par exemple, pour la détection de micro-organismes pathogènes d'origine alimentaire dans des prélèvements environnementaux ainsi que dans les aliments pour animaux.

Mikrobiologija živil in krme - Odkrivanje prisotnosti patogenih mikroorganizmov v živilih s polimerazno verižno reakcijo (PCR) v realnem času - Splošne zahteve in definicije (ISO 22119:2011)

Ta mednarodni standard določa izraze za odkrivanje prisotnosti patogenih mikroorganizmov v živilih in izolatih, pridobljenih iz njih, z uporabo polimerazne verižne reakcije (PRC). Ta mednarodni standard tudi opredeljuje zahteve za povečevanje in odkrivanje prisotnosti zaporedij nukleinskih kislin (DNK ali RNK po reverzni transkripciji) s PRC v realnem času. Minimalne zahteve, določene v tem mednarodnem standardu, zagotavljajo temelj za primerljive in ponovljive rezultate v posameznem laboratoriju in med različnimi laboratoriji. Ta mednarodni standard se uporablja tudi, na primer, za odkrivanje prisotnosti patogenih mikroorganizmov v živilih v okoljskih vzorcih in v krmah.

General Information

• Status: Published
• Publication Date: 14-Jul-2011
• Withdrawal Date: 15-Jan-2012
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463/WG 1 - General requirements relating to PCR methods
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Jul-2011
• Due Date: 04-Apr-2010
• Completion Date: 15-Jul-2011
• Ref Project: SIST EN ISO 22119:2011 - Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 22119:2011
01-oktober-2011
0LNURELRORJLMDåLYLOLQNUPH2GNULYDQMHSULVRWQRVWLSDWRJHQLKPLNURRUJDQL]PRYY
åLYLOLKVSROLPHUD]QRYHULåQRUHDNFLMR3&5YUHDOQHPþDVX6SORãQH]DKWHYHLQ
GHILQLFLMH,62
Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction
(PCR) for the detection of food-borne pathogens - General requirements and definitions
(ISO 22119:2011)
Mikrobiologie von Lebensmitteln und Futtermitteln - Real-Time-Polymerase-
Kettenreaktion (PCR) für den Nachweis von pathogenen Mikroorganismen in
Lebensmitteln - Allgemeine Anforderungen und Begriffe (ISO 22119:2011)
Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) en temps réel
pour la détection des micro-organismes pathogènes dans les aliments - Exigences
générales et définitions (ISO 22119:2011)
Ta slovenski standard je istoveten z: EN ISO 22119:2011
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 22119:2011 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 22119:2011

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SIST EN ISO 22119:2011


EUROPEAN STANDARD
EN ISO 22119

NORME EUROPÉENNE

EUROPÄISCHE NORM
July 2011
ICS 07.100.30
English Version
Microbiology of food and animal feeding stuffs - Real-time
polymerase chain reaction (PCR) for the detection of food-borne
pathogens - General requirements and definitions (ISO
22119:2011)
Microbiologie des aliments - Réaction de polymérisation en Mikrobiologie von Lebensmitteln und Futtermitteln - Real-
chaîne (PCR) en temps réel pour la détection des micro-
time-Polymerase-Kettenreaktion (PCR) zum Nachweis von
organismes pathogènes dans les aliments - Exigences pathogenen Mikroorganismen in Lebensmitteln -
générales et définitions (ISO 22119:2011) Allgemeine Anforderungen und Begriffe (ISO 22119:2011)
This European Standard was approved by CEN on 14 July 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22119:2011: E
worldwide for CEN national Members.

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SIST EN ISO 22119:2011
EN ISO 22119:2011 (E)
Contents Page
Foreword .3

2

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SIST EN ISO 22119:2011
EN ISO 22119:2011 (E)
Foreword
This document (EN ISO 22119:2011) has been prepared by Technical Committee CEN/TC 275 “Food
analysis - Horizontal methods”, the secretariat of which is held by DIN, in collaboration with Technical
Committee ISO/TC 34 "Food products".
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at
the latest by January 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland
...