EN ISO 20837:2006

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection (ISO 20837:2006)

ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.
It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.
ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

Mikrobiologie von Lebensmitteln und Futtermitteln - Polymerase-Kettenreaktion (PCR) zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Anforderungen an die Probenvorbereitung für den qualitativen Nachweis (ISO 20837:2006)

Diese Internationale Norm liefert Kriterien und Beispiele für die Probenvorbereitung, um für die PCR geeignete Proben oder Nukleinsäuren von für die PCR geeigneter Qualität und Quantität zu erhalten.
Sie liefert eine Beschreibung der in Frage kommenden allgemeinen Prinzipien, gefolgt von Verweisungen auf ISO-Normen (Anhang A) und einem ausführlich beschriebenen Verfahren der DNS-Extraktion (Anhang B).
Diese Internationale Norm wurde für Lebensmittelproben aufgestellt, gegebenenfalls kann sie auch mit einigen Anpassungen auf Futtermittel- und landwirtschaftliche/Umweltproben angewendet werden.

Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) pour la détection des micro-organismes pathogènes dans les aliments - Exigences relatives à la préparation des échantillons pour la détection qualitative (ISO 20837:2006)

L'ISO 20837:2006 fournit des critères et des exemples de préparation d'échantillons permettant d'obtenir des échantillons compatibles avec la méthode PCR ou des acides nucléiques de qualité appropriée et en quantité suffisante pour la méthode PCR.
Elle contient une description des principes généraux mis en oeuvre. Les références de normes relatives à l'enrichissement de micro-organismes sont données dans l'Annexe A et une méthode détaillée d'extraction de l'ADN est donnée dans l'Annexe B.
L'ISO 20837:2006 a été élaborée pour des matrices alimentaires mais pourrait également être appliquée à des aliments pour animaux et à des matrices agricoles/environnementales avec quelques adaptations, si nécessaire.

Mikrobiologija živil in krme – Odkrivanje prisotnosti patogenih mikroorganizmov v živilih z metodo verižne reakcije s polimerazo (PCR) – Zahteve za pripravo vzorca za kvalitativno odkrivanje (ISO 20837:2006)

General Information

• Status: Published
• Publication Date: 14-Apr-2006
• Withdrawal Date: 30-Oct-2006
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463/WG 1 - General requirements relating to PCR methods
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Apr-2006
• Completion Date: 15-Apr-2006
• Ref Project: SIST EN ISO 20837:2006 - Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection (ISO 20837:2006)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 20837:2006
01-september-2006
Mikrobiologija živil in krme – Odkrivanje prisotnosti patogenih mikroorganizmov v
živilih z metodo verižne reakcije s polimerazo (PCR) – Zahteve za pripravo vzorca
za kvalitativno odkrivanje (ISO 20837:2006)
Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the
detection of food-borne pathogens - Requirements for sample preparation for qualitative
detection (ISO 20837:2006)
Mikrobiologie von Lebensmitteln und Futtermitteln - Polymerase-Kettenreaktion (PCR)
zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Anforderungen an
die Probenvorbereitung für den qualitativen Nachweis (ISO 20837:2006)
Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) pour la
détection des micro-organismes pathogenes dans les aliments - Exigences relatives a la
préparation des échantillons pour la détection qualitative (ISO 20837:2006)
Ta slovenski standard je istoveten z: EN ISO 20837:2006
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 20837:2006 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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EUROPEAN STANDARD
EN ISO 20837
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2006
ICS 07.100.30

English Version
Microbiology of food and animal feeding stuffs - Polymerase
chain reaction (PCR) for the detection of food-borne pathogens -
Requirements for sample preparation for qualitative detection
(ISO 20837:2006)
Microbiologie des aliments - Réaction de polymérisation en Mikrobiologie von Lebensmitteln und Futtermitteln -
chaîne (PCR) pour la détection des micro-organismes Polymerase-Kettenreaktion (PCR) zum Nachweis von
pathogènes dans les aliments - Exigences relatives à la pathogenen Mikroorganismen in Lebensmitteln -
préparation des échantillons pour la détection qualitative Anforderungen an die Probenvorbereitung bei qualitativem
(ISO 20837:2006) Nachweis (ISO 20837:2006)
This European Standard was approved by CEN on 13 April 2006.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20837:2006: E
worldwide for CEN national Members.

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EN ISO 20837:2006 (E)





Foreword


This document (EN ISO 20837:2006) has been prepared by Technical Committee CEN/TC 275
"Food analysis - Horizontal methods", the secretariat of which is held by DIN, in collaboration
with Technical Committee ISO/TC 34 "Agricultural food products".

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by October 2006, and conflicting national
standards shall be withdrawn at the latest by October 2006.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.


2

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INTERNATIONAL ISO
STANDARD 20837
First edition
2006-04-15

Microbiology of food and animal feeding
stuffs — Polymerase chain reaction
(PCR) for the detection of food-borne
pathogens — Requirements for sample
preparation for qualitative detection
Microbiologie des aliments — Réaction de polymérisation en chaîne
(PCR) pour la détection des micro-organismes pathogènes dans les
aliments — Exigences relatives à la préparation des échantillons pour la
détection qualitative

...