EN ISO 22174:2005

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22174:2005)

ISO 22174:2004 gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR)
The minimum requirements laid down in ISO 22174:2004 are intended to ensure that comparable and reproducible results are obtained in different laboratories.
ISO 22174:2004 has been established for food-borne pathogens in or isolated from food and feed matrices, but is also applicable to other matrices (e.g. environmental samples) and for the detection of non-pathogenic microorganisms.

Mikrobiologie von Lebensmitteln und Futtermitteln - Polymerase- Kettenreaktion (PCR) zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Allgemeine Anforderungen und Begriffe (ISO 22174:2005)

Diese Internationale Norm beschreibt allgemeine Anforderungen an die in-vitro-Vervielfältigung (Amplifikation) von Nukleinsäuresequenzen (DNS oder RNS). Sie gilt für die Untersuchung von Lebensmitteln und daraus gewonnenen Isolaten auf mikrobielle Kontamination unter Anwendung der Polymerase-Kettenreaktion (PCR).
Die in dieser Internationalen Norm festgelegten Mindestanforderungen dienen dazu, vergleichbare und reproduzierbare Ergebnisse in verschiedenen Laboren sicherzustellen.
Diese Internationale Norm wurde für den Nachweis von pathogenen Mikroorganismen in oder isoliert aus Lebensmittel- und Futtermittelmatrices erarbeitet. Sie kann jedoch auch auf andere Matrices, z. B. Umweltproben, und zum Nachweis nicht pathogener Mikroorganismen angewendet werden.

Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) pour la recherche de micro-organismes pathogènes dans les aliments - Exigences générales et définitions (ISO 22174:2005)

L'ISO 22174:2005 s'applique aux essais d'aliments et aux extraits obtenus à partir de ces aliments pour la détection de micro-organismes pathogènes en faisant appel à la réaction de polymérisation en chaîne (PCR) et stipule les exigences générales relatives à l'amplification in vitro des séquences d'acide nucléique (ADN ou ARN).
Les exigences minimales déclarées dans l'ISO 22174:2005 sont destinées à garantir l'obtention de résultats comparables et reproductibles dans des laboratoires différents.
L'ISO 22174:2005 a été conçue pour les micro-organismes pathogènes présents dans les aliments ou extraits d'aliments et de matrices d'aliments mais elle peut également s'appliquer à d'autres matrices, comme les échantillons environnementaux, ainsi que pour la détection de micro-organismes non pathogènes.

Mikrobiologija živil in krme - Prisotnost patogenih mikroorganizmov v živilih z metodo verižne reakcije s polimerazo (PCR) - Splošne zahteve in definicije (ISO 22174:2005)

General Information

• Status: Published
• Publication Date: 14-Feb-2005
• Withdrawal Date: 30-Aug-2005
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463/WG 1 - General requirements relating to PCR methods
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 12-Jun-2009
• Completion Date: 12-Jun-2009
• Ref Project: SIST EN ISO 22174:2005 - Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22174:2005)

Relations

• Revised: prEN ISO 22174 - Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection and quantification of microorganisms - General requirements and definitions (ISO/DIS 22174:2022)
• Effective Date: 30-Dec-2020

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 22174:2005
01-junij-2005
Mikrobiologija živil in krme - Prisotnost patogenih mikroorganizmov v živilih z
metodo verižne reakcije s polimerazo (PCR) - Splošne zahteve in definicije (ISO
22174:2005)
Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the
detection of food-borne pathogens - General requirements and definitions (ISO
22174:2005)
Mikrobiologie von Lebensmitteln und Futtermitteln - Polymerase- Kettenreaktion (PCR)
zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Allgemeine
Anforderungen und Begriffe (ISO 22174:2005)
Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) pour la
recherche de micro-organismes pathogenes dans les aliments - Exigences générales et
définitions (ISO 22174:2005)
Ta slovenski standard je istoveten z: EN ISO 22174:2005
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 22174:2005 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 22174:2005

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SIST EN ISO 22174:2005



EUROPEAN STANDARD
EN ISO 22174

NORME EUROPÉENNE

EUROPÄISCHE NORM
February 2005
ICS 07.100.30
English version
Microbiology of food and animal feeding stuffs - Polymerase
chain reaction (PCR) for the detection of food-borne pathogens -
General requirements and definitions (ISO 22174:2005)
Microbiologie des aliments - Réaction de polymérisation en Mikrobiologie von Lebensmitteln und Futtermitteln -
chaîne (PCR) pour la recherche de micro-organismes Polymerase- Kettenreaktion (PCR) zum Nachweis von
pathogènes dans les aliments - Exigences générales et pathogenen Mikroorganismen in Lebensmitteln -
définitions (ISO 22174:2005) Allgemeine Anforderungen und Begriffe (ISO 22174:2005)
This European Standard was approved by CEN on 20 October 2004.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.

CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.



EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22174:2005: E
worldwide for CEN national Members.

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SIST EN ISO 22174:2005
EN ISO 22174:2005 (E)




Foreword



This document (EN ISO 22174:2005) has been prepared by Technical Committee CEN/TC 275 "Food
analysis - Horizontal methods", the secretariat of which is held by DIN, in collaboration with Technical
Committee ISO/TC 34 "Agricultural food products".

This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by August 2005, and conflicting national standards shall be withdrawn
at the latest by August 2005.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and United Kingdom.

2

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SIST EN ISO 22174:2005


INTERNATIONAL ISO
STANDARD 22174
First edition
2005-02-15


Microbiology of food and animal feeding
stuffs — Polymerase chain reaction
(PCR) for the detection of food-borne
pathogens — General requirements and
definitions
Microbiologie des aliments — Réaction de polymérisation en chaîne
(PCR) pour la recherche de micro-organismes pathogènes dans les
aliments — Exigences générales et définitions




Reference number
ISO 22174:2005(E)
©
ISO 2005

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