EN ISO 20838:2006

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for amplification and detection for qualitative methods (ISO 20838:2006)

ISO 20838:2006 provides the overall framework for qualitative methods for the detection of food-borne pathogens using the polymerase chain reaction (PCR).
It covers the general requirements for the specific amplification of target nucleic acid sequences and the detection and confirmation of the identity of the amplified nucleic acid sequence.
Guidelines, minimum requirements and performance characteristics described in ISO 20838:2006 are intended to ensure that comparable and reproducible results are obtained in different laboratories.
ISO 20838:2006 has been established for food-borne pathogens in or isolated from food and feed matrices, but can also be applied to other matrices, for example environmental samples, or to the detection of other microorganisms under investigation.

Mikrobiologie von Lebensmitteln und Futtermitteln - Polymerase-Kettenreaktion (PCR) zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Anforderungen an Amplifikation und Nachweis bei qualitativen Verfahren (ISO 20838:2006)

Diese Internationale Norm legt Rahmenbedingungen für die qualitativen Verfahren zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln mittels der Polymerase-Kettenreaktion (PCR) fest.
Sie bezieht sich auf die allgemeinen Anforderungen an die spezifische Amplifikation von Target-Nukleinsäuresequenzen und den Nachweis sowie die Bestätigung der Identität der amplifizierten Nukleinsäuresequenz.
Die in dieser Internationalen Norm genannten Anleitungen, Mindestanforderungen und Leistungskriterien dienen dazu sicherzustellen, dass in verschiedenen Laboratorien vergleichbare und reproduzierbare Ergebnisse erhalten werden.
Diese Internationale Norm dient zum Nachweis von pathogenen Mikroorganismen in Lebensmittel- und Futtermittelproben oder aus ihnen isolierten pathogenen Mikroorganismen. Sie kann jedoch auch bei anderen Matrices, wie z. B. Umweltproben oder zum Nachweis von anderen in Untersuchung befindlichen Mikroorganismen, angewendet werden.

Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) pour la détection des micro-organismes pathogènes dans les aliments - Exigences relatives à l'amplification et à la détection pour les méthodes qualitatives (ISO 20838:2006)

L'ISO 20838:2006 fournit le cadre de travail général des méthodes qualitatives pour la détection des micro-organismes pathogènes présents dans les aliments à l'aide de la réaction de polymérisation en chaîne (PCR).
Elle concerne les exigences générales relatives à l'amplification spécifique des séquences d'acide nucléique cible et à la détection et la confirmation de la nature de la séquence d'acide nucléique amplifiée.
Les lignes directrices, les exigences minimales et les caractéristiques de performances décrites dans l'ISO 20838:2006 visent à garantir l'obtention de résultats comparables et reproductibles dans des laboratoires différents.
L'ISO 20838:2006 a été conçue pour les micro-organismes pathogènes présents dans les aliments et les matrices alimentaires ou isolés de ces derniers, mais elle peut également s'appliquer à d'autres matrices, par exemple les échantillons environnementaux, ainsi que pour la détection d'autres micro-organismes.

Mikrobiologija živil in krme – Odkrivanje prisotnosti patogenih mikroorganizmov v živilih z metodo verižne reakcije s polimerazo (PCR) – Zahteve za povečanje in odkrivanje pri kvalitativnih metodah (ISO 20838:2006)

General Information

• Status: Published
• Publication Date: 14-Apr-2006
• Withdrawal Date: 30-Oct-2006
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463/WG 1 - General requirements relating to PCR methods
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-Apr-2006
• Completion Date: 15-Apr-2006
• Ref Project: SIST EN ISO 20838:2006 - Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for amplification and detection for qualitative methods (ISO 20838:2006)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 20838:2006
01-september-2006
0LNURELRORJLMDåLYLOLQNUPH±2GNULYDQMHSULVRWQRVWLSDWRJHQLKPLNURRUJDQL]PRYY
åLYLOLK]PHWRGRYHULåQHUHDNFLMHVSROLPHUD]R3&5±=DKWHYH]DSRYHþDQMHLQ
RGNULYDQMHSULNYDOLWDWLYQLKPHWRGDK,62
Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the
detection of food-borne pathogens - Requirements for amplification and detection for
qualitative methods (ISO 20838:2006)
Mikrobiologie von Lebensmitteln und Futtermitteln - Polymerase-Kettenreaktion (PCR)
zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Anforderungen an
Amplifikation und Nachweis bei qualitativen Verfahren (ISO 20838:2006)
Microbiologie des aliments - Réaction de polymérisation en chaîne (PCR) pour la
détection des micro-organismes pathogenes dans les aliments - Exigences relatives a
l'amplification et a la détection pour les méthodes qualitatives (ISO 20838:2006)
Ta slovenski standard je istoveten z: EN ISO 20838:2006
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 20838:2006 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

EUROPEAN STANDARD
EN ISO 20838
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2006
ICS 07.100.30

English Version
Microbiology of food and animal feeding stuffs - Polymerase
chain reaction (PCR) for the detection of food-borne pathogens -
Requirements for amplification and detection for qualitative
methods (ISO 20838:2006)
Microbiologie des aliments - Réaction de polymérisation en Mikrobiologie von Lebensmitteln und Futtermitteln -
chaîne (PCR) pour la détection des micro-organismes Polymerase-Kettenreaktion (PCR) zum Nachweis von
pathogènes dans les aliments - Exigences relatives à pathogenen Mikroorganismen in Lebensmitteln -
l'amplification et à la détection pour les méthodes Anforderungen an Amplifikation und Nachweis bei
qualitatives (ISO 20838:2006) qualitativen Verfahren (ISO 20838:2006)
This European Standard was approved by CEN on 13 April 2006.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20838:2006: E
worldwide for CEN national Members.

---------------------- Page: 2 ----------------------

EN ISO 20838:2006 (E)





Foreword


This document (EN ISO 20838:2006) has been prepared by Technical Committee CEN/TC 275
"Food analysis - Horizontal methods", the secretariat of which is held by DIN, in collaboration
with Technical Committee ISO/TC 34 "Agricultural food products".

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by October 2006, and conflicting national
standards shall be withdrawn at the latest by October 2006.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.


2

---------------------- Page: 3 ----------------------

INTERNATIONAL ISO
STANDARD 20838
First edition
2006-04-15

Microbiology of food and animal feeding
stuffs — Polymerase chain reaction
(PCR) for the detection of food-borne
pathogens — Requirements for
amplification and detection for qualitative
methods
Microbiologie des aliments — Réaction de polymérisation en chaîne
(PCR) pour la détection des micro-organismes pathogènes dans les
aliments — Exigences relatives à l'amplification et à la dé
...