Analytical Method for the Analysis of Propiconazole in treated Wood Samples

This CEN Technical Report specifies a laboratory method for determining the content of propiconazole in treated wood using either Gas Chromatography (GC) or High Performance Liquid Chromatography (HPLC).
The method is aiming at determining the treatment quality at the time of treatment.
NOTE 1   Under appropriate circumstances the method is applicable for tebuconazole-treated wood as well as for the analysis of waste timber with respect to its propiconazole content.
The method has a detection limit lower than 1 µg propiconazole/g and a quantification limit corresponding to 30 µg propiconazole/g of wood material expressed as dry matter. It can be used over a measurement range up to a propiconazole content of 600 µg/g of dry matter.
NOTE 2   This method may need some modifications with some wood species such as hardwoods.

Analyseverfahren zum Nachweis von Propiconazol in behandelten Holzproben

Dieser Europäische Technische Bericht legt ein Laborverfahren zur Bestimmung des Gehalts an Propiconazol
in behandeltem Holz unter Anwendung entweder der Gaschromatographie (GC) oder der
Hochleistungsflüssigkeitschromatographie (HPLC) fest.
Das Verfahren zielt darauf ab, die Qualität der Behandlung zum Zeitpunkt dieser Behandlung zu bestimmen.
ANMERKUNG 1 Unter den geeigneten Umständen ist das Verfahren sowohl auf mit Tebuconazol behandeltes Holz als
auch für die Analyse von Altholz in Bezug auf dessen Propiconazol-Gehalt anwendbar.
Das Verfahren weist eine Nachweisgrenze von weniger als 1 µg Propiconazol/g und eine Bestimmungsgrenze
entsprechend 30 µg Propiconazol/g Holzmaterial auf, angegeben als Trockenmasse. Es kann über einen
Messbereich bis zu einem Propiconazolgehalt von 600 µg/g Trockenmasse angewendet werden.
ANMERKUNG 2 Dieses Verfahren erfordert bei einigen Holzarten, wie z. B. Laubhölzern, möglicherweise bestimmte
Veränderungen.

Méthode d'analyse du propiconazole dans des échantillons de bois traité

Le présent Rapport technique spécifie une méthode de détermination en laboratoire de la teneur en
propiconazole présent dans le bois traité, au moyen de la chromatographie en phase gazeuse (GC) ou de la
chromatographie liquide à haute performance (HPLC).
La méthode vise à déterminer la qualité du traitement au moment du traitement.
NOTE 1 La méthode est applicable, dans des conditions appropriées, tant au bois traité au tébuconazole qu’à l’analyse
du bois de rebut en ce qui concerne la teneur en propiconazole.
La limite de détection de la méthode est inférieure à 1 mg de propiconazole/g de bois et sa limite de
quantification correspond à 30 mg de propiconazole/g de bois exprimé en matière sèche. La méthode peut
être utilisée pour une teneur en propiconazole comprise dans une plage de mesures allant jusqu’à 600 mg de
propiconazole/g de matière sèche.
NOTE 2 La présente méthode peut nécessiter des modifications quand elle est utilisée sur des essences de bois
comme les bois de feuillus.

Analitska metoda za analizo propikonazola v obdelanih vzorcih lesa

To tehnično poročilo CEN določa laboratorijsko metodo za ugotavljanje vsebnosti propikonazola v obdelanem lesu s plinsko kromatografijo (GC) ali tekočinsko kromatografijo visoke ločljivosti (HPLC). Cilj metode je ugotoviti kakovost obdelave v času obdelave. Meja detekcije metode je nižja od 1 μg propikonazola na gram, meja kvantifikacije pa ustreza 30 μg propikonazola na gram lesnega materiala, izraženega s suho snovjo. Uporabi se lahko za merilno območje do 600 μg vsebnosti propikonazola na gram suhe snovi.

General Information

Status
Published
Publication Date
23-Oct-2012
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Due Date
24-Oct-2012
Completion Date
24-Oct-2012

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SLOVENSKI STANDARD
SIST-TP CEN/TR 16420:2013
01-januar-2013
Analitska metoda za analizo propikonazola v obdelanih vzorcih lesa
Analytical Method for the Analysis of Propiconazole in Treated Wood Samples
Analyseverfahren zum Nachweis von Propiconazol in behandelten Holzproben
Méthode d'analyse du propiconazole dans des échantillons de bois traité
Ta slovenski standard je istoveten z: CEN/TR 16420:2012
ICS:
79.040 Les, hlodovina in žagan les Wood, sawlogs and sawn
timber
SIST-TP CEN/TR 16420:2013 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TP CEN/TR 16420:2013
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SIST-TP CEN/TR 16420:2013
TECHNICAL REPORT
CEN/TR 16420
RAPPORT TECHNIQUE
TECHNISCHER BERICHT
October 2012
ICS 79.040
English Version
Analytical Method for the Analysis of Propiconazole in treated
Wood Samples

Méthode d'analyse du propiconazole dans des échantillons Analyseverfahren zum Nachweis von Propiconazol in

de bois traité behandelten Holzproben

This Technical Report was approved by CEN on 27 August 2012. It has been drawn up by the Technical Committee CEN/TC 38.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United

Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2012 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TR 16420:2012: E

worldwide for CEN national Members.
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CEN/TR 16420:2012 (E)
Contents Page

Foreword ..............................................................................................................................................................3

1 Scope ......................................................................................................................................................4

2 Normative references ............................................................................................................................4

3 Safety precautions .................................................................................................................................4

4 Principle ..................................................................................................................................................5

5 Reagents .................................................................................................................................................5

6 Apparatus ...............................................................................................................................................5

7 Test sample ............................................................................................................................................6

7.1 General ....................................................................................................................................................6

7.2 Dry matter content .................................................................................................................................6

8 Analytical procedure .............................................................................................................................6

8.1 General ....................................................................................................................................................6

8.2 Standard solutions ................................................................................................................................7

8.3 Determination .........................................................................................................................................7

9 Calculation and expression of results .................................................................................................9

10 Quality assurance ..................................................................................................................................9

11 Precision .................................................................................................................................................9

12 Test report ........................................................................................................................................... 10

Bibliography ..................................................................................................................................................... 11

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SIST-TP CEN/TR 16420:2013
CEN/TR 16420:2012 (E)
Foreword

This document (CEN/TR 16420:2012) has been prepared by Technical Committee CEN/TC 38 “Durability of

wood and wood-based products”, the secretariat of which is held by AFNOR.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

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SIST-TP CEN/TR 16420:2013
CEN/TR 16420:2012 (E)
1 Scope

This CEN Technical Report specifies a laboratory method for determining the content of propiconazole in

treated wood using either Gas Chromatography (GC) or High Performance Liquid Chromatography (HPLC).

The method is aiming at determining the treatment quality at the time of treatment.

NOTE 1 Under appropriate circumstances the method is applicable for tebuconazole-treated wood as well as for the

analysis of waste timber with respect to its propiconazole content.

The method has a detection limit lower than 1 µg propiconazole/g and a quantification limit corresponding to

30 µg propiconazole/g of wood material expressed as dry matter. It can be used over a measurement range

up to a propiconazole content of 600 µg/g of dry matter.

NOTE 2 This method may need some modifications with some wood species such as hardwoods.

2 Normative references

The following documents, in whole or in part, are normatively referenced in this document and are

indispensable for its application. For dated references, only the edition cited applies. For undated references,

the latest edition of the referenced document (including any amendments) applies.

EN 212, Wood preservatives – General guidance on sampling and preparation for analysis of wood

preservatives and treated timber

EN 351-2, Durability of wood and wood-based products – Preservative treated solid wood – Part 2: Guidance

on sampling for the analysis of preservative-treated wood

EN 13183-1, Moisture content of a piece of sawn timber – Part 1: Determination by oven dry method

3 Safety precautions

Persons using this method should be familiar with normal analytical laboratory procedures and practice.

This method does not purport to address all safety problems, if any, associated with its use.

It is the responsibility of the user to establish safety and health practices and to ensure compliance with any

European or national regulatory conditions.
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4 Principle

Propiconazole is quantitatively extracted from the wood material using methanol. The extracted compounds

are analysed by GC-ECD, GC-MS, GC-NPD, HPLC-UV or HPLC-DAD.

NOTE Other analytical techniques have been reported to be adequately applicable. However, when using other

analytical techniques the comparability to the method described in this CEN Technical Report should be demonstrated.

1) 2)
Neither FID nor NPD detectors are expected to be appropriate detecting devices.
5 Reagents
5.1 General

During the analysis, unless otherwise specified, use only reagents of recognised analytical grade that have

been checked in advance as to not interfere with the analytical results.
5.2 Methanol (CH OH), analytical grade.

NOTE Other solvents may be used instead of methanol (e.g. ethylacetate) as some extraction difficulties may occur

with some wood species (e.g. hardwoods). It is recommended to cross check the extraction efficiency of any other solvent

or solvent mixture with that of methanol.
5.3 Propiconazole [C H Cl N O ] [CAS 60207-90-1] of certified purity:
15 17 2 3 2

 purity >98 % - mixture of stereo isomers (e.g. Pestanal from Sigma-Aldrich, cat. 45642),

 alternatively, (typical purity 50 %) - mixture of stereo isomers, from Janssen Pharmaceutica, supplied

with Certificate of Analysis.
6 Apparatus
Ordinary laboratory apparatus and the following:

6.1 Grinder, capable of grinding timber samples such that the resultant particles will pass through a 1000

µm sieve.
6.2 Analytical balance, accurate to 0,1 mg.

6.3 Ultra-sonic bath equipped with a thermostat capable of controlling and maintaining a temperature of

(50 ± 1) °C.

6.4 Adjustable volumetric micropipettes, 10 µl, 100 µl and 1000 µl full capacity.

6.5 Volumetric pipettes, 10 ml capacity.
6.6 Volumetric flasks, 50 ml capacity.

6.7 Glass tubes 30 ml capacity, with screw caps provided with an insert of polytetrafluoroethylene (PTFE)

or alternatively with standard ground stoppers provided with PTFE standard ground sockets and conical joint

clips.
1) Concluded from the 2007 round robin test.
2) Concluded from the 2008 round robin test.
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6.8 PTFE filters 0,45 µm porosity with supporting device.
6.9 2 ml GC vials with sealed caps.

6.10 Gas chromatograph equipped with a splitless/split or a non-discriminating injection system and an

electron capture detector (ECD) for example.

6.11 Semi-polar separation column. One capillary column, or preferably two with a stationary phase of

different polarity; leng
...

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