Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-3:2017)

ISO 18363-3:2017 specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis.
ISO 18363-3:2017 is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.

Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 3: Verfahren mittels Säureumesterung und Messung für 2 MCPD, 3 MCPD und Glycidol (ISO 18363-3:2017)

Dieses Dokument legt ein Verfahren für die gleichzeitige Bestimmung von 2 MCPD-Estern (gebundenem 2 MCPD), 3 MCPD-Estern (gebundenem 3 MCPD) und Glycidylestern (gebundenem Glycidol) in einem einzigen Assay fest, basierend auf einer vor der GC/MS-Analyse erfolgenden säurekatalysierten Esterspaltung und Derivatisierung der abgespaltenen (freien) Analyten mit Phenylboronsäure (en: phenylboronic acid, PBA).
Dieses Dokument ist auf feste und flüssige Fette und Öle anwendbar. Für alle drei Analyten liegt die Quantifizierungsgrenze (LOQ, en: limit of quantification) bei 0,1 mg/kg und die Nachweisgrenze (LOD, en: limit of detection) bei 0,03 mg/kg.

Corps gras d'origines animale et végétale - Détermination des esters de chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras par CPG/SM - Partie 3: Méthode par transestérification acide et mesure du 2-MCPD, du 3-MCPD et du glycidol (ISO 18363-3:2017)

ISO 18363-3:2017 spécifie un mode opératoire permettant la détermination simultanée des esters de 2-MCPD (2-MCPD lié), des esters de 3-MCPD (3-MCPD lié) et des esters de glycidol (glycidol lié) en un seul essai, basé sur le clivage des esters par catalyse acide et la dérivatisation des analytes clivés (libres) à l'acide phénylboronique (PBA) avant analyse par CPG/SM.
ISO 18363-3:2017 est applicable aux corps gras solides et liquides. Pour l'ensemble des trois analytes, la limite de quantification (LOQ) est de 0,1 mg/kg et la limite de détection (LOD) est de 0,03 mg/kg.

Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih kloropropandiolov (MCPD) in glicidola z GC/MS - 3. del: Metoda z uporabo kislinske transesterifikacije in meritev 2-MCPD, 3-MCPD in glicidola (ISO 18363-3:2017)

Ta dokument določa postopek za hkratno določevanje estrov 2-MCPD (vezan 2-MCPD), estrov 3-MCPD (vezan 3-MCPD) in glicidil estrov (vezan glicidol) v enem samem testu, ki temelji na cepljenju estrov, kataliziranih s kislino, in derivatizaciji razcepljenih (prostih) analitov s fenilboronsko kislino (PBA) pred analizo GC/MS.
Ta dokument se uporablja za trdne in tekoče maščobe ter olja. Za vse tri analite je meja kvantifikacije (LOQ) 0,1 mg/kg, meja zaznavanja (LOD) pa 0,03 mg/kg.

General Information

Status
Published
Publication Date
14-Dec-2021
Withdrawal Date
29-Jun-2022
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
15-Dec-2021
Due Date
27-Oct-2023
Completion Date
15-Dec-2021

Buy Standard

Standard
EN ISO 18363-3:2022
English language
27 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day
Draft
prEN ISO 18363-3:2021
English language
24 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 18363-3:2022
01-februar-2022
Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih
kloropropandiolov (MCPD) in glicidola z GC/MS - 3. del: Metoda z uporabo
kislinske transesterifikacije in meritev 2-MCPD, 3-MCPD in glicidola (ISO 18363-
3:2017)
Animal and vegetable fats and oils - Determination of fatty-acid-bound
chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid
transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-
3:2017)
Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen
Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 3: Verfahren mittels
Säureumesterung und Messung für 2 MCPD, 3 MCPD und Glycidol (ISO 18363 3:2017)
Corps gras d'origines animale et végétale - Détermination des esters de
chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras
par CPG/SM - Partie 3: Méthode par transestérification acide et mesure du 2-MCPD, du
3-MCPD et du glycidol (ISO 18363-3:2017)
Ta slovenski standard je istoveten z: EN ISO 18363-3:2021
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
SIST EN ISO 18363-3:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 18363-3:2022

---------------------- Page: 2 ----------------------
SIST EN ISO 18363-3:2022


EN ISO 18363-3
EUROPEAN STANDARD

NORME EUROPÉENNE

December 2021
EUROPÄISCHE NORM
ICS 67.200.10
English Version

Animal and vegetable fats and oils - Determination of fatty-
acid-bound chloropropanediols (MCPDs) and glycidol by
GC/MS - Part 3: Method using acid transesterification and
measurement for 2-MCPD, 3-MCPD and glycidol (ISO
18363-3:2017)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination des esters de chloropropanediols von fettsäuregebundenen Chlorpropandiol (MCPD)
(MCPD) et d'acides gras et des esters de glycidol et und Glycidol mittels GC/MS - Teil 3: Verfahren mittels
d'acides gras par CPG/SM - Partie 3: Méthode par Säureumesterung und Messung für 2 MCPD, 3 MCPD
transestérification acide et mesure du 2-MCPD, du 3- und Glycidol (ISO 18363 3:2017)
MCPD et du glycidol (ISO 18363-3:2017)
This European Standard was approved by CEN on 5 December 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18363-3:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN ISO 18363-3:2022
EN ISO 18363-3:2021 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------
SIST EN ISO 18363-3:2022
EN ISO 18363-3:2021 (E)
European foreword
The text of ISO 18363-3:2017 has been prepared by Technical Committee ISO/TC 34 "Food products” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 18363-
3:2021 by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-
products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2022, and conflicting national standards shall be
withdrawn at the latest by June 2022.
Attention is drawn to the possibi
...

SLOVENSKI STANDARD
oSIST prEN ISO 18363-3:2021
01-oktober-2021
Živalske in rastlinske maščobe ter olja - Določevanje maščobnokislinsko vezanih
kloropropandiolov (MCPD) in glicidola z GC/MS - 3. del: Metoda z uporabo
kislinske transesterifikacije in meritev 2-MCPD, 3-MCPD in glicidola (ISO 18363-
3:2017)
Animal and vegetable fats and oils - Determination of fatty-acid-bound
chloropropanediols (MCPDs) and glycidol by GC/MS - Part 3: Method using acid
transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO 18363-
3:2017)
Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenen
Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 3: Verfahren mittels
Säureumesterung und Messung für 2 MCPD, 3 MCPD und Glycidol (ISO 18363 3:2017)
Corps gras d'origines animale et végétale - Détermination des esters de
chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras
par CPG/SM - Partie 3: Méthode par transestérification acide et mesure du 2-MCPD, du
3-MCPD et du glycidol (ISO 18363-3:2017)
Ta slovenski standard je istoveten z: prEN ISO 18363-3
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
oSIST prEN ISO 18363-3:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN ISO 18363-3:2021

---------------------- Page: 2 ----------------------
oSIST prEN ISO 18363-3:2021
INTERNATIONAL ISO
STANDARD 18363-3
First edition
2017-12
Animal and vegetable fats and oils —
Determination of fatty-acid-bound
chloropropanediols (MCPDs) and
glycidol by GC/MS —
Part 3:
Method using acid transesterification
and measurement for 2-MCPD,
3-MCPD and glycidol
Corps gras d'origines animale et végétale — Détermination des
esters de chloropropanediols (MCPD) et d'acides gras et des esters de
glycidol et d'acides gras par CPG/SM —
Partie 3: Méthode par transestérification acide et mesure pour le
2-MCPD, le 3-MCPD et le glycidol
Reference number
ISO 18363-3:2017(E)
©
ISO 2017

---------------------- Page: 3 ----------------------
oSIST prEN ISO 18363-3:2021
ISO 18363-3:2017(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2017 – All rights reserved

---------------------- Page: 4 ----------------------
oSIST prEN ISO 18363-3:2021
ISO 18363-3:2017(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
5.1 Standard and reference compounds . 2
5.2 Standard solutions . 3
5.2.1 G
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.