oSIST prEN ISO 11133:2025
(Main)Microbiology of the food chain, animal feed and water - Preparation, production, storage and performance testing of culture media and reagents (ISO/DIS 11133:2025)
Microbiology of the food chain, animal feed and water - Preparation, production, storage and performance testing of culture media and reagents (ISO/DIS 11133:2025)
Mikrobiologie der Lebensmittelkette, Futtermittel und Wasser - Vorbereitung, Herstellung, Lagerung und Leistungsprüfung von Nährmedien und Reagenzien (ISO/DIS 11133:2025)
Microbiologie de la chaîne alimentaire, des aliments pour animaux et de l’eau - Préparation, production, stockage et essais de performance des milieux de culture et des réactifs (ISO/DIS 11133:2025)
Mikrobiologija v prehranski verigi, živalski krmi in vodi - Priprava, izdelava, skladiščenje in preskušanje lastnosti gojišč in reagentov (ISO/DIS 11133:2025)
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Standards Content (Sample)
SLOVENSKI STANDARD
01-september-2025
Nadomešča:
SIST EN ISO 11133:2014
SIST EN ISO 11133:2014/A1:2018
SIST EN ISO 11133:2014/A2:2020
Mikrobiologija v prehranski verigi, živalski krmi in vodi - Priprava, izdelava,
skladiščenje in preskušanje lastnosti gojišč in reagentov (ISO/DIS 11133:2025)
Microbiology of the food chain, animal feed and water - Preparation, production, storage
and performance testing of culture media and reagents (ISO/DIS 11133:2025)
Mikrobiologie der Lebensmittelkette, Futtermittel und Wasser - Vorbereitung,
Herstellung, Lagerung und Leistungsprüfung von Nährmedien und Reagenzien (ISO/DIS
11133:2025)
Microbiologie de la chaîne alimentaire, des aliments pour animaux et de l’eau -
Préparation, production, stockage et essais de performance des milieux de culture et
des réactifs (ISO/DIS 11133:2025)
Ta slovenski standard je istoveten z: prEN ISO 11133
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
DRAFT
International
Standard
ISO/DIS 11133
ISO/TC 34/SC 9
Microbiology of the food chain,
Secretariat: AFNOR
animal feed and water —
Voting begins on:
Preparation, production, storage
2025-06-23
and performance testing of culture
Voting terminates on:
media and reagents
2025-09-15
ICS: 07.100.30; 07.100.20
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
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147/SC 4 before casting their ballot to the e-Balloting application.
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IN ADDITION TO THEIR EVALUATION AS
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Reference number
ISO/DIS 11133:2025(en)
DRAFT
ISO/DIS 11133:2025(en)
International
Standard
ISO/DIS 11133
ISO/TC 34/SC 9
Microbiology of the food chain,
Secretariat: AFNOR
animal feed and water —
Voting begins on:
Preparation, production, storage
and performance testing of culture
Voting terminates on:
media and reagents
ICS: 07.100.30; 07.100.20
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
Member bodies are requested to consult relevant national interests in ISO/TC AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
147/SC 4 before casting their ballot to the e-Balloting application.
PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
This document is circulated as received from the committee secretariat.
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2025
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
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NATIONAL REGULATIONS.
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Published in Switzerland Reference number
ISO/DIS 11133:2025(en)
ii
ISO/DIS 11133:2025(en)
Contents Page
Foreword .vi
Introduction .viii
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
3.1 General terms and definitions .2
3.2 Terminology of performance testing .2
3.3 Terminology of culture media .3
3.4 Terminology for test microorganisms .6
3.5 Terminology for reagents and supplements .8
4 Quality assurance management . 8
4.1 Documentation .8
4.1.1 Documentation from manufacturer or producer .8
4.1.2 Delivery acceptance of products .8
4.2 Storage .9
4.2.1 General .9
4.2.2 Quality management and product control of dehydrated culture media and
supplements.9
4.2.3 Quality management and product control of ready‐to‐use culture media .9
4.2.4 Quality management and product control of reagents .9
4.3 Laboratory preparation of culture media and reagents .10
4.3.1 General .10
4.3.2 Quality of basic culture medium components .10
4.3.3 Water .10
4.3.4 Weighing and rehydration .11
4.3.5 Dissolution and dispersion .11
4.3.6 Measurement and adjustment of pH .11
4.3.7 Dispensing .11
4.3.8 Sterilization . . .11
4.3.9 Preparation of supplements . 12
4.4 Storage and shelf-life of prepared culture media and reagents. 12
4.4.1 Commercially supplied culture media and reagents . 12
4.4.2 Laboratory prepared culture media and reagents . 12
4.5 Preparation for use . 13
4.5.1 Melting of agar culture media . 13
4.5.2 De-aeration of culture media .14
4.5.3 Addition of supplements .14
4.5.4 Preparation of solid and semi-solid culture media in Petri dishes .14
4.5.5 Preparation of plated culture media for inoculation .14
4.6 Incubation of solid and semi-solid culture media in Petri dishes . 15
4.7 Disposal of culture media and reagents . 15
5 Test microorganisms for performance testing .15
5.1 General . 15
5.2 Selection of test microorganisms . 15
5.3 Preservation and maintenance of test microorganisms .16
5.3.1 General .16
5.3.2 Test microorganisms from commercial sources .16
5.3.3 Laboratory prepared reference stocks.16
5.3.4 Stock cultures.16
5.3.5 Working cultures .17
5.4 Microorganisms for performance testing .17
5.4.1 General .17
5.4.2 Preparation .17
iii
ISO/DIS 11133:2025(en)
6 Quality control and performance testing of culture media and reagents .20
6.1 General requirements . 20
6.2 Physical and chemical quality control .
...
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