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SIST EN 14176:2004

(Main)

Foodstuffs - Determination of domoic acid in mussels by HPLC

Foodstuffs - Determination of domoic acid in mussels by HPLC

This European Standard specifies a method for the determination of domoic acid in mussels using high performance liquid chromatography (HPLC).
The method has been successfully validated in an interlaboratory study according to AOAC guidelines on mussels containing 14,1 mg/g (spiked sample) to 186 mg/g (naturally contaminated sample) domoic acid.
Laboratory experiences show that the method is also applicable on the common cockle (Cerastoderma edule), the peppery furrow shell (Scrobicularia plana), clams (Venerupis pullastra, Ruditapes decussata), oyster (Crassostrea japonica) and razor clams (Ensis spp., Solen spp) [1].

Lebensmittel - Bestimmung von Domoinsäure in Muscheln mit HPLC

Diese Europäische Norm legt ein Verfahren für die Bestimmung von Domoinsäure in Muscheln mit Hochleistungs-Flüssigchromatographie (HPLC) fest.
Das Verfahren wurde erfolgreich in einem Ringversuch nach den AOAC-Richtlinien an Muscheln validiert, die 14,1 µg/g (aufgestockt) bis 186 µg/g (natürlich kontaminiert) Domoinsäure enthielten.
Erfahrungen im Labor zeigen, dass das Verfahren auch auf Herzmuscheln (Cerastoderma edule), Pfeffermuscheln (Scrobicularia plana), Venus-Muscheln (Venerupis pullastra, Ruditapes decussata), Austern (Crassostrea japonica) und Scheidenmuscheln (Ensis spp., Solen spp.) anwendbar ist [1].

Produits alimentaires - Dosage de l'acide domoique dans les moules par CLHP

Le présent projet de Norme européenne spécifie une méthode de dosage de l'acide domoique dans les moules, par chromatographie liquide haute performance (CLHP).
La méthode a été validée avec succes lors d'une étude interlaboratoires réalisée conformément aux lignes directrices de l'AOAC sur des moules contenant de 14,1 µg/g (échantillon supplémenté) a 186 µg/g (échantillon naturellement contaminé) d'acide domoique.
Des expériences de laboratoire montrent que la méthode est aussi applicable sur la coque commune (Cerastoderma edule), le scrobiculaire (Scrobicularia plana), la palourde (Venerupis pullastra, Ruditapes decussata), l'huître (Crassostrea japonica) et le couteau (Ensis spp., Solen spp) [1].

Živila – Določevanje domojske kisline v školjkah s HPLC

General Information

Status
Withdrawn
Publication Date
30-Apr-2004
Withdrawal Date
19-Feb-2017
ICS
67.120.30 - Fish and fishery products
Technical Committee
KŽP - Agricultural food products
Current Stage
9900 - Withdrawal (Adopted Project)
Start Date
20-Feb-2017
Due Date
15-Mar-2017
Completion Date
20-Feb-2017
Ref Project
EN 14176:2003 - Foodstuffs - Determination of domoic acid in mussels by HPLC

Relations

Replaced By
SIST EN 14176:2017 - Foodstuffs - Determination of domoic acid in raw shellfish, raw finfish and cooked mussels by RP-HPLC using UV detection
Effective Date
01-Mar-2017

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Domoinsäure in Muscheln mit HPLCProduits alimentaires - Dosage de l'acide domoique dans les moules par CLHPFoodstuffs - Determination of domoic acid in mussels by HPLC67.120.30Ribe in ribji proizvodiFish and fishery productsICS:Ta slovenski standard je istoveten z:EN 14176:2003SIST EN 14176:2004en01-maj-2004SIST EN 14176:2004SLOVENSKI
STANDARD



SIST EN 14176:2004



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14176December 2003ICS 67.120.30English versionFoodstuffs - Determination of domoic acid in mussels by HPLCProduits alimentaires - Dosage de l'acide domoïque dansles moules par CLPHLebensmittel - Bestimmung von Domoinsäure in Muschelnmit HPLCThis European Standard was approved by CEN on 1 October 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14176:2003 ESIST EN 14176:2004



EN 14176:2003 (E)2ContentsPageForeword.31Scope.42Normative references.43Principle.44Reagents.45Apparatus.56Sampling.57Procedure.58Calculation.69Precision.710Test report.7Annex A (informative)
Precision data.9Bibliography.10SIST EN 14176:2004



EN 14176:2003 (E)3ForewordThis document (EN 14176:2003) has been prepared by Technical Committee CEN/TC 275 “Food analysis -Horizontal methods”, the secretariat of which is held by DIN.This European Standard shall be given the status of a national standard, either by publication of an identicaltext or by endorsement, at the latest by June 2004, and conflicting national standards shall be withdrawn atthe latest by June 2004.Annex A in this document is informative.WARNING —The use of this standard can involve hazardous materials, operations and equipment.This standard does not purport to address all the safety problems associated with its use. It is theresponsibility of the user of this standard to establish appropriate safety and health practices anddetermine the applicability of regulatory limitations prior to use.According to the CEN/CENELEC Internal
...

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