SIST EN 16930:2017
(Main)Animal feeding stuffs: Methods of sampling and analysis - Determination of carbadox and olaquindox by HPLC/UV
Animal feeding stuffs: Methods of sampling and analysis - Determination of carbadox and olaquindox by HPLC/UV
This European Standard specifies a high performance liquid chromatographic – UV detection (HPLC-UV) method for the simultaneous determination of two growth promoters Carbadox and Olaquindox contents in compound feeds and raw materials at levels ranging from the limit of quantification to 100 mg kg-1.
The limit of quantification of the method has been demonstrated to be better than 3 mg kg-1 for olaquindox and 4 mg kg-1 for carbadox.
Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von Carbadox und Olaquindox mittels HPLC/UV
Diese Europäische Norm legt ein Verfahren zur gleichzeitigen Bestimmung des jeweiligen Gehalts der beiden Wachstumsförderer Carbadox und Olaquindox in Mischfuttermitteln und Rohmaterialien bei Konzentrationen im Bereich der Bestimmungsgrenze (en: limit of quantification, LOQ) bis zu 100 mg kg−1 mit Hochleistungs¬flüssigkeitschromatographie und UV Detektion (HPLC-UV) fest.
Die Bestimmungsgrenze des Verfahrens hat gezeigt, dass sie besser als 3 mg kg−1 bei Olaquindox und 4 mg kg−1 bei Carbadox ist.
Aliments des animaux - Méthodes d'échantillonnage et d'analyse - Détermination des teneurs en carbadox et olaquindox par CLHP/UV
La présente Norme européenne spécifie une méthode de chromatographie liquide à haute performance avec détection UV (CLHP-UV) pour la détermination simultanée des teneurs en carbadox et olaquindox, deux promoteurs de croissance, dans les aliments composés pour animaux et les matières premières à des niveaux compris entre la limite de quantification et 100 mg kg-1.
La limite de quantification de la méthode s’est révélée être inférieure à 3 mg kg-1 pour l’olaquindox et inférieure à 4 mg kg-1 pour le carbadox.
Krma: metode vzorčenja in analize - Določevanje karbadoksa in olakvindoksa s HPLC/UV
Ta evropski standard določa metodo tekočinske kromatografije visoke ločljivosti z UV-detekcijo (HPLC-UV) za hkratno določanje vsebnosti dveh pospeševalcev rasti karbadoksa in olakvindoksa v krmnih mešanicah in surovinah pri koncentracijah od mejne vrednosti kvantifikacije do 100 mg kg-1.
Mejna vrednost kvantifikacije metode je bila dokazano boljša od 3 mg kg-1 za olakvindoks in 4 mg kg-1 za karbadoks.
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.+3/&89Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von Carbadox und Olaquindox mittels HPLC/UVAliments des animaux - Méthodes d'échantillonnage et d'analyse - Détermination des teneurs en carbadox et olaquindox par CLHP/UVAnimal feeding stuffs: Methods of sampling and analysis - Determination of carbadox and olaquindox by HPLC/UV71.040.50Fizikalnokemijske analitske metodePhysicochemical methods of analysis65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 16930:2017SIST EN 16930:2017en,fr,de01-september-2017SIST EN 16930:2017SLOVENSKI
STANDARD
SIST EN 16930:2017
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16930
July
t r s y ICS
x wä s t r English Version
Animal feeding stuffsã Methods of sampling and analysis æ Determination of carbadox aAliments des animaux æ Méthodes d 5échantillonnage et d 5analyse æ Détermination des teneurs en carbadox et
Futtermittel æ Probenahmeæ und Untersuchungsverfahren æ Bestimmung von Carbadox This European Standard was approved by CEN on
s v May
t r s yä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Serbiaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
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9
t r s y CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s x { u rã t r s y ESIST EN 16930:2017
EN 16930:2017 (E) 2 Contents Page European foreword . 4 1 Scope . 5 2 Normative references . 5 3 Principle . 5 4 Reagents and materials . 5 4.1 Water, demineralized or deionized or at least equivalent . 6 4.2 Methanol . 6 4.3 Acetonitrile . 6 4.4 Glacial acetic acid, minimum purity 96 % . 6 4.5 Ammonium acetate water free salt, CH3CO2NH4 . 6 4.6 Extraction solvent: methanol water mixture (1:1 v:v) . 6 4.7 Ammonium acetate buffer, 25 mM, pH = 4,35 . 6 4.8 Mobile phase for HPLC: acetonitrile: buffer mixture (10:90; v:v) . 6 4.9 Carbadox dissolving solvent: methanol: acetonitrile mixture (1:1; v:v) . 6 4.10 Reference standards . 6 4.11 Standard solutions . 6 4.12 Neutral aluminium oxide, Brockmann activity I . 8 4.13 Neutral aluminium oxide SP
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