EN ISO 10927:2018

Plastics - Determination of the molecular mass and molecular mass distribution of polymer species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) (ISO 10927:2018)

This document specifies a general method for determining the average molecular mass and molecular mass distribution of polymers (see Reference [1]) from 2 000 g ⋅ mol−1 to 20 000 g ⋅ mol−1 by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
The average molecular masses and molecular mass distributions are calculated from a calibration curve constructed using synthetic-polymer and/or biopolymer standards. This method is therefore classified as a relative method.
The method is not applicable to polyolefins or to polymers with a polydispersity >1,2.

Kunststoffe - Bestimmung der Molmasse und Molmassenverteilung von polymeren Species durch matrixunterstütze Laser-Desorptions/Ionisations-Flugzeit-Massenspektrometrie (MALDI-TOF-MS) (ISO 10927:2018)

Dieses Dokument legt ein allgemeines Verfahren zum Bestimmen der mittleren Molmasse und der
Molmassenverteilung von Polymeren (siehe Literaturhinweis [1]) in einem Molmassenbereich von
2 000 g ⋅ mol−1 bis 20 000 g ⋅ mol−1 durch die matrixunterstützte Laser-Desorptions/Ionisations-Flugzeit-
Massenspektrometrie (MALDI-TOF-MS) fest.
Die mittleren Molmassen und Molmassenverteilungen werden über eine Kalibrierkurve berechnet, die mit
Hilfe von synthetischen und/oder Biopolymerstandards erstellt wird. Daher handelt es sich um eine relative
Methode.
Das Verfahren ist nicht für Polyolefine oder Polymere mit einer Polydispersität > 1,2 anwendbar.

Plastiques - Détermination de la masse moléculaire et de la distribution des masses moléculaires des polymères par spectrométrie de masse, à temps de vol, après désorption/ionisation laser assistée par matrice (SM-MALDI-TOF) (ISO 10927:2018)

Le présent document spécifie une méthode générale permettant de déterminer la masse moléculaire moyenne et la distribution des masses moléculaires moyennes de polymères (voir Référence [1]) comprises entre 2 000 g ⋅ mol−1 et 20 000 g ⋅ mol−1 par spectrométrie de masse, à temps de vol, après désorption/ionisation laser assistée par matrice (SM-MALDI-TOF).
Les masses moléculaires et les distributions des masses moléculaires moyennes sont calculées à partir d'une courbe d'étalonnage établie à l'aide d'étalons de polymères synthétiques et/ou de biopolymères. Par conséquent, la présente méthode est classée comme méthode relative.
La présente méthode n'est pas applicable aux polyoléfines et aux polymères ayant une polydispersité > 1,2.

Polimerni materiali - Ugotavljanje molekulske mase in porazdelitve molekulske mase polimerov z masno spektrometrijo po laserski desorpciji/ionizaciji v nosilcu (matriksu) (MALDI-TOF-MS) (ISO 10927:2018)

Ta dokument določa splošno metodo za ugotavljanje povprečne molekulske mase in porazdelitve molekulske mase polimerov (glej sklic [1]) od 2000 g ⋅ mol−1 do 20.000 g ⋅ mol−1 z masno spektrometrijo po laserski desorpciji/ionizaciji v nosilcu (matriksu) (MALDI-TOF-MS).
Povprečne molekulske mase in porazdelitve molekulskih mas se izračunajo na podlagi kalibracijske krivulje, izdelane z uporabo standardov za sintetični polimer in/ali biopolimer. Ta metoda je zato klasificirana kot relativna metoda.
Metoda se ne uporablja za poliolefine oziroma polimere s polidisperzijo > 1,2.

General Information

• Status: Published
• Publication Date: 10-Jul-2018
• Withdrawal Date: 30-Jan-2019
• ICS: 83.080.01 - Plastics in general
• Technical Committee: CEN/TC 139 - Paints and varnishes
• Drafting Committee: CEN/TC 139 - Paints and varnishes
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 11-Jul-2018
• Completion Date: 11-Jul-2018
• Ref Project: SIST EN ISO 10927:2018 - Plastics - Determination of the molecular mass and molecular mass distribution of polymer species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) (ISO 10927:2018)

Relations

• Replaces: EN ISO 10927:2011 - Plastics - Determination of the molecular mass and molecular mass distribution of polymer species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) (ISO 10927:2011)
• Effective Date: 26-Oct-2016

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2018
1DGRPHãþD
SIST EN ISO 10927:2011
Polimerni materiali - Ugotavljanje molekulske mase in porazdelitve molekulske
mase polimerov z masno spektrometrijo po laserski desorpciji/ionizaciji v nosilcu
(matriksu) (MALDI-TOF-MS) (ISO 10927:2018)
Plastics - Determination of the molecular mass and molecular mass distribution of
polymer species by matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF-MS) (ISO 10927:2018)
Kunststoffe - Bestimmung der Molmasse und Molmassenverteilung von polymeren
Species durch matrixunterstütze Laser-Desorptions/Ionisations-Flugzeit-
Massenspektrometrie (MALDI-TOF-MS) (ISO 10927:2018)
Plastiques - Détermination de la masse moléculaire et de la distribution des masses
moléculaires des polymères par spectrométrie de masse, à temps de vol, après
désorption/ionisation laser assistée par matrice (SM-MALDI-TOF) (ISO 10927:2018)
Ta slovenski standard je istoveten z: EN ISO 10927:2018
ICS:
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
83.080.01 Polimerni materiali na Plastics in general
splošno
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 10927
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2018
EUROPÄISCHE NORM
ICS 83.080.01 Supersedes EN ISO 10927:2011
English Version
Plastics - Determination of the molecular mass and
molecular mass distribution of polymer species by matrix-
assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF-MS) (ISO 10927:2018)
Plastiques - Détermination de la masse moléculaire et Kunststoffe - Bestimmung der Molmasse und
de la distribution des masses moléculaires des Molmassenverteilung von polymeren Species durch
polymères par spectrométrie de masse, à temps de vol, matrixunterstütze Laser-Desorptions/Ionisations-
après désorption/ionisation laser assistée par matrice Flugzeit-Massenspektrometrie (MALDI-TOF-MS) (ISO
(SM-MALDI-TOF) (ISO 10927:2018) 10927:2018)
This European Standard was approved by CEN on 28 June 2018.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10927:2018 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 10927:2018) has been prepared by Technical Committee ISO/TC 61 "Plastics"
in collaboration with Technical Committee CEN/TC 139 “Paints and varnishes” the secretariat of which
is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2019, and conflicting national standards shall
be withdrawn at the latest by January 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 10927:2011.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 10927:2018 has been approved by CEN as EN ISO 10927:2018 without any modification.

INTERNATIONAL ISO
STANDARD 10927
Second edition
2018-06
Plastics — Determination of the
molecular mass and molecular mass
distribution of polymer species by
matrix-assisted laser desorption/
ionization time-of-flight mass
spectrometry (MALDI-TOF-MS)
Plastiques — Détermination de la masse moléculaire et de la
distribution des masses moléculaires des polymères par spectrométrie
de masse, à temps de vol, après désorption/ionisation laser assistée
par matrice (SM-MALDI-TOF)
Reference number
ISO 10927:2018(E)
©
ISO 2018
ISO 10927:2018(E)
© ISO 2018
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii © ISO 2018 – All rights reserved

ISO 10927:2018(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
5.1 Matrices . 2
5.2 Solvents . 2
5.3 Salts . 3
5.4 Molecular mass standards . 3
6 Apparatus . 3
6.1 General . 3
6.2 Sample introduction chamber/target . 3
6.3 Laser source . 4
6.4 Flight tube . 4
6.5 Detector . 4
6.6 Data recording . 4
6.7 Data handling . 5
7 Procedure. 5
7.1 General . 5
7.2 Sample preparation . 5
7.2.1 General. 5
7.2.2 Preparation of polymer/matrix/salt solutions . 5
7.2.3 Deposition of the sample on the sample plate (target) . 5
7.2.4 Preparation and spotting of biopolymer/matrix solutions . 6
7.3 Instrument settings . 6
7.4 Recording spectra . 7
8 Data acquisition and processing . 7
8.1 General . 7
8.2 Calibration . 8
8.2.1 General. 8
8.2.2 Calibration of mass axis using synthetic-polymer standards . 8
8.2.3 Calibration of mass axis using biopolymer standards . 8
8.2.4 Self-calibration method . 8
8.3 Generation of calibration curve . 8
8.4 Signal intensity axis calibration . 8
9 Expression of results . 9
9.1 Calculation of molecular mass distribution . 9
9.2 Calculation of the average molecular masses .
...