ISO 23611-2:2024

Soil quality — Sampling of soil invertebrates — Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina)

This document specifies a method for sampling, extracting and preserving collembolans and mites from field soils as a prerequisite for using these animals as bio-indicators (e.g. to assess the quality of a soil as a habitat for organisms). The sampling and extraction methods of this document are applicable to almost all types of soils. Exceptions can be soils from extreme climatic conditions (hard, frozen or flooded soils) and other matrices than soil, e.g. tree trunks, plants or lichens.

Qualité du sol — Prélèvement des invertébrés du sol — Partie 2: Prélèvement et extraction des micro-arthropodes (Collembola et Acarina)

Le présent document spécifie une méthode pour le prélèvement, l’extraction et la conservation des collemboles et des acariens du sol prélevés sur le terrain comme prérequis à l’utilisation de ces animaux en tant que bio-indicateurs (par exemple pour évaluer la qualité d’un sol en tant qu’habitat pour les organismes). Les méthodes de prélèvement et d’extraction du présent document s’appliquent à la quasi-totalité des sols. Les sols présents sous des conditions climatiques extrêmes (sols durs, gelés ou inondés) et les matrices autres que le sol, à l’instar des troncs d’arbres, des plantes ou des lichens, peuvent être considérés comme des exceptions.

General Information

• Status: Published
• Publication Date: 23-Apr-2024
• ICS: 13.080.05 - Examination of soils in general
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: ISO/TC 190/SC 4 - Biological characterization
• Drafting Committee: ISO/TC 190/SC 4 - Biological characterization
• Current Stage: 6060 - International Standard published
• Start Date: 24-Apr-2024
• Due Date: 17-Jun-2024
• Completion Date: 24-Apr-2024

Relations

• Revises: ISO 23611-2:2006 - Soil quality — Sampling of soil invertebrates — Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina)
• Effective Date: 18-Jun-2022

Standards Content (Sample)

International
Standard
ISO 23611-2
Second edition
Soil quality — Sampling of soil
2024-04
invertebrates —
Part 2:
Sampling and extraction of micro-
arthropods (Collembola and
Acarina)
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 2: Prélèvement et extraction des micro-arthropodes
(Collembola et Acarina)
Reference number
ISO 23611-2:2024(en) © ISO 2024

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ISO 23611-2:2024(en)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland

© ISO 2024 – All rights reserved
ii

---------------------- Page: 2 ----------------------
ISO 23611-2:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Test materials . 2
5.1 Biological material .2
5.2 Reagents .2
6 Apparatus . 3
7 Procedure . 4
7.1 Collecting the soil samples . .4
7.2 Extracting Collembola and Acarina from soil samples .4
7.3 Sorting, preserving and identifying Collembola and Acarina .5
7.3.1 Sorting and preserving .5
7.3.2 Identification .5
8 A ssessment of results . 6
9 Study report .
...

Norme
internationale
ISO 23611-2
Deuxième édition
Qualité du sol — Prélèvement des
2024-04
invertébrés du sol —
Partie 2:
Prélèvement et extraction des
micro-arthropodes (Collembola et
Acarina)
Soil quality — Sampling of soil invertebrates —
Part 2: Sampling and extraction of micro-arthropods (Collembola
and Acarina)
Numéro de référence
ISO 23611-2:2024(fr) © ISO 2024

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ISO 23611-2:2024(fr)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2024
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: [phone]
E-mail: [e-mail]
Web: www.iso.org
Publié en Suisse

© ISO 2024 – Tous droits réservés
ii

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ISO 23611-2:2024(fr)
Sommaire Page
Avant-propos .iv
Introduction .v
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe. 1
5 Matériaux d’essai . 2
5.1 Matériel biologique .2
5.2 Réactifs .2
6 Appareillage . 3
7 Mode opératoire . 4
7.1 Collecte des échantillons de sol .4
7.2 Extraction des collemboles et des acariens des échantillons de sol .5
7.3 Tri, conservation et identification des collemboles et des acariens .6
7.3.1 Tri et conservation . . .6
7.3.2 Identification .6
8 Évaluation des résultats . 7
9 Rapport d’étude .
...

FINAL DRAFT
International
Standard
ISO/FDIS 23611-2
ISO/TC 190/SC 4
Soil quality — Sampling of soil
Secretariat: AFNOR
invertebrates —
Voting begins on:
2024-02-02
Part 2:
Sampling and extraction of micro-
Voting terminates on:
2024-03-29
arthropods (Collembola and
Acarina)
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 2: Prélèvement et extraction des micro-arthropodes
(Collembola et Acarina)
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 23611-2:2024(en) © ISO 2024

---------------------- Page: 1 ----------------------
FINAL DRAFT
ISO/FDIS 23611-2:2024(en)
International
Standard
ISO/FDIS 23611-2
ISO/TC 190/SC 4
Soil quality — Sampling of soil
Secretariat: AFNOR
invertebrates —
Voting begins on:
Part 2:
Sampling and extraction of micro-
Voting terminates on:
arthropods (Collembola and
Acarina)
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 2: Prélèvement et extraction des micro-arthropodes
(Collembola et Acarina)
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
COPYRIGHT PROTECTED DOCUMENT
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2024
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland Reference number
ISO/FDIS 23611-2:2024(en) © ISO 2024

© ISO 2024 – All rights reserved
ii

---------------------- Page: 2 ----------------------
ISO/FDIS 23611-2:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Test materials . 2
5.1 Biological material .2
5.2 Reagents .2
6 Apparatus .
...

2023-12-15
ISO/DIS FDIS 23611-2:2023(E)
ISO/TC 190/SC 4
Secretariat: AFNOR
Date: 2024-01-19
Soil quality — Sampling of soil invertebrates —
—
Part 2:
Sampling and extraction of micro-arthropods (Collembola and
Acarina)
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 2 : Prélèvement et extraction des micro-arthropodes (Collembola et Acarina)
Partie 2: Prélèvement et extraction des micro-arthropodes (Collembola et Acarina)
FDIS stage

---------------------- Page: 1 ----------------------
ISO/DIS FDIS 23611-2:2023(E2024(en)
© ISO 2023, Published in Switzerland2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. Phone: + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail: [e-mail]
Website: www.iso.org
Published in Switzerland
© ISO 2024 – All rights reserved

ii © ISO 2023 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/DIS FDIS 23611-2:2023(E2024(en)
Contents
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Test materials . 2
5.1 Biological material . 2
5.2 Reagents . 2
6 Apparatus . 3
7 Procedure . 4
7.1 Collecting the soil samples . 4
7.2 Extracting Collembola and Acarina from soil samples . 4
7.3 Sorting, preserving and identifying Collembola and Acarina . 5
7.3.1 Sorting and preserving . 5
7.3.2 Identification . 5
8 Assessment of results . 6
9 Study report . 7
Annex A (informative) Species determination in collembolans and mites .
...