EN ISO 14673-3:2001

Milk and milk products - Determination of nitrate and nitrite contents - Part 3: Method using cadmium reduction and flow injection analysis with in-line dialysis (Routine method) (ISO 14673-3:2001)

TC forecasts updated : delay with the directives ( TC forecasts updating exercise.sj 00-11-09)++

Milch und Milchprodukte - Bestimmung des Nitrat- und Nitritgehaltes - Teil 3: Verfahren mit Cadmiumreduktion und Fließinjektionsanalyse mit In-line-Dialyse (Routineverfahren) (ISO14673-3:2001)

Dieser Teil von ISO 14673/IDF 189 legt ein Verfahren zur Bestimmung des Nitrat- und Nitritgehaltes von Milch und Milchprodukten durch Cadmiumreduktion und Fließinjektionsanalyse (FIA) fest. Das Verfahren gilt für Hart-, Schnitt- und Weichkäse unterschiedlichen Alters und für Schmelzkäse. Die Nachweisgrenzen des Verfahrens liegen bei 0,5 mg Nitrat-Ionen je Kilogramm und bei 1,0 mg Nitrit-Ionen je Kilogramm.
Das Verfahren gilt auch für Molkenpulver, Milchpulver und Säuglings- und Kleinkindernahrung auf Milchbasis.
ANMERKUNG 1   Das Verfahren ähnelt sehr dem in [2] beschriebenen FIA-Verfahren zur Bestimmung von Nitrat und Nitrit in Milch und flüssigen Molkereiprodukten. Um die Analyse von Käse zu ermöglichen und um eine ausreichende Empfindlichkeit für die Bestimmung geringer Nitritkonzentrationen in Käse und Säuglings- und Kleinkindernahrung auf Milchbasis zu erreichen, wurden Anpassungen vorgenommen.
ANMERKUNG 2   Für die Nitrit- und Nitratbestimmung mit Cadmiumreduktion wird die gleiche Farbreaktion verwendet, wie sie in ISO 14673-1/IDF 189-1 beschrieben ist.

Lait et produits laitiers - Détermination des teneurs en nitrates et en nitrites - Partie 3: Méthode par réduction au cadmium et d'analyse par injection avec dialyse en ligne (Méthode de routine) (ISO 14673-3:2001)

Mleko in mlečni proizvodi - Določevanje nitratov in nitritov - 3. del: Metoda uporabe redukcije s kadmijem in pretočna tehnika z dializo (rutinska metoda) (ISO 14673-3:2001)

General Information

• Status: Withdrawn
• Publication Date: 31-Oct-2001
• Withdrawal Date: 31-Jan-2004
• ICS: 67.100.01 - Milk and milk products in general
• Technical Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Drafting Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Start Date: 01-Feb-2004
• Completion Date: 01-Feb-2004
• Ref Project: SIST EN ISO 14673-3:2002 - Milk and milk products - Determination of nitrate and nitrite contents - Part 3: Method using cadmium reduction and flow injection analysis with in-line dialysis (Routine method) (ISO 14673-3:2001)

Relations

• Replaced By: EN ISO 14673-3:2004 - Milk and milk products - Determination of nitrate and nitrite contents - Part 3: Method using cadmium reduction and flow injection analysis with in-line dialysis (Routine method) (ISO 14673-3:2004)
• Effective Date: 22-Dec-2008
• Corrected By: EN ISO 14673-3:2001/AC:2002 - Milk and milk products - Determination of nitrate and nitrite contents - Part 3: Method using cadmium reduction and flow injection analysis with in-line dialysis (Routine method) (ISO 14673-3:2001)
• Effective Date: 22-Dec-2008

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 14673-3:2002
01-junij-2002
0OHNRLQPOHþQLSURL]YRGL'RORþHYDQMHQLWUDWRYLQQLWULWRYGHO0HWRGD
XSRUDEHUHGXNFLMHVNDGPLMHPLQSUHWRþQDWHKQLND]GLDOL]RUXWLQVNDPHWRGD,62

Milk and milk products - Determination of nitrate and nitrite contents - Part 3: Method
using cadmium reduction and flow injection analysis with in-line dialysis (Routine
method) (ISO 14673-3:2001)
Milch und Milchprodukte - Bestimmung des Nitrat- und Nitritgehaltes - Teil 3: Verfahren
mit Cadmiumreduktion und Fließinjektionsanalyse mit In-line-Dialyse (Routineverfahren)
(ISO14673-3:2001)
Lait et produits laitiers - Détermination des teneurs en nitrates et en nitrites - Partie 3:
Méthode par réduction au cadmium et d'analyse par injection avec dialyse en ligne
(Méthode de routine) (ISO 14673-3:2001)
Ta slovenski standard je istoveten z: EN ISO 14673-3:2001
ICS:
67.100.01 0OHNRLQPOHþQLSURL]YRGLQD Milk and milk products in
VSORãQR general
SIST EN ISO 14673-3:2002 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN ISO 14673-3:2002

---------------------- Page: 2 ----------------------

SIST EN ISO 14673-3:2002
EUROPEAN STANDARD
EN ISO 14673-3
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2001
ICS 67.100.01
English version
Milk and milk products - Determination of nitrate and nitrite
contents - Part 3: Method using cadmium reduction and flow
injection analysis with in-line dialysis (Routine method) (ISO
14673-3:2001)
Lait et produits laitiers - Détermination des teneurs en Milch und Milchprodukte - Bestimmung des Nitrat- und
nitrates et en nitrites - Partie 3: Méthode par réduction au Nitritgehaltes - Teil 3: Verfahren mit Cadmiumreduktion
cadmium et d'analyse par injection avec dialyse en ligne und Fließinjektionsanalyse mit In-line-Dialyse
(Méthode de routine) (ISO 14673-3:2001) (Routineverfahren) (ISO14673-3:2001)
This European Standard was approved by CEN on 1 November 2001.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14673-3:2001 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST EN ISO 14673-3:2002
EN ISO 14673-3:2001 (E)
CORRECTED 2002-02-06
Foreword
This document (ISO 14673-3:2001) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 302 "Milk and
milk products - Methods of sampling and analysis", the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by May 2002, and conflicting national
standards shall be withdrawn at the latest by May 2002.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United
Kingdom.
Endorsement notice
The text of the International Standard ISO 14673-3:2001 has been approved by CEN as a
European Standard without any modifications.
NOTE Normative references to International Standards are listed in annex ZA (normative).
2

---------------------- Page: 4 ----------------------

SIST EN ISO 14673-3:2002
EN ISO 14673-3:2001 (E)
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions
of any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications,
indicated by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 1042 1998 Laboratory glassware – One-mark EN ISO 1042 1999
volumetric flasks
ISO 14673-1 2001 Milk and milk products - EN ISO 14673-1 2001
Determination of nitrate and nitrite
contents - Part 1: Method using
cadmium reduction and
spectrometry
3

---------------------- Page: 5 ----------------------

SIST EN ISO 14673-3:2002

---------------------- Page: 6 ----------------------

SIST EN ISO 14673-3:2002
INTERNATIONAL ISO
STANDARD 14673-3
IDF
189-3
First edition
2001-11-01
Milk and milk products — Determination
of nitrate and nitrite contents —
Part 3:
Method using cadmium reduction and flow
injection analysis with in-line dialysis
(Routine method)
Lait et produits laitiers — Détermination des teneurs en nitrates et en
nitrites —
Partie 3: Méthode par réduction au cadmium et d'analyse par injection avec
dialyse en ligne (Méthode de routine)
Reference numbers
ISO 14673-3:2001(E)
IDF 189-3:2001(E)
©
ISO and IDF 2001

---------------------- Page: 7 ----------------------

SIST EN ISO 14673-3:2002
ISO 14673-3:2001(E)
IDF 189-3:2001(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not
be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this
file, parties accept therein the responsibility of not infringing Adobe's licensing policy. Neither the ISO Central Secretariat nor the IDF accepts
any liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters
were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies and IDF national
committees. In the unlikely event that a problem relating to it is found, please inform the ISO Central Secretariat at the address given below.
© ISO and IDF 2001
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic
or mechanical, including photocopying and microfilm, without permission in writing from either ISO or IDF at the respective address below.
ISO copyright office International Dairy Federation
Case postale 56 � CH-1211 Geneva 20 41 Square Vergote � B-1030 Brussels
Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88
Fax + 41 22 749 09 47 Fax + 32 2 733 04 13
E-mail [e-mail] E-mail [e-mail]
Web www.iso.ch Web www.fil-idf.org
Printed in Switzerland
ii © ISO and IDF 2001 – All rights reserved

---------------------- Page: 8 ----------------------

SIST EN ISO 14673-3:2002
ISO 14673-3:2001(E)
IDF 189-3:2001(E)
Contents Page
Foreword.iv
1 Scope .1
2 Normative references .1
3 Terms and definitions .1
4 Principle.2
5 Reagents.2
6 Apparatus .3
7 Sampling.5
8 Preparation of test sample.5
8.1 Cheese .5
9 Procedure .6
9.1 Checking the reducing capacity of the cadmium column.6
9.2 Regeneration of the cadmium column .6
9.3 Test portion .6
9.4 Extraction .6
9.5 Determination of nitrite content .7
9.6 Determination of nitrate content .7
10 Calculation and expression of results.7
10.1 Nitrite content.7
10.2 Nitrate content.8
11 Precision.8
11.1 General.8
11.2 Nitrite content.8
11.3 Nitrate content.9
12 Test report .9
Bibliography.10
© ISO and IDF 2001 – All rights reserved iii

---------------------- Page: 9 ----------------------

SIST EN ISO 14673-3:2002
ISO 14673-3:2001(E)
IDF 189-3:2001(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this part of ISO 14673�IDF 189 may be the subject
of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 14673-3�IDF 189-3 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with
AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International.
This first edition of ISO 14673-3�IDF 189-3, together with ISO 14673-1�IDF 189-1 and ISO 14673-2�IDF 189-2,
cancels and replaces ISO 4099:1984, ISO 6736:1982, ISO 6739:1988, ISO 6740:1985 and ISO 8195:1987, which
have been technically revised.
ISO 14673�IDF 189 consists of the following parts, under the general title Milk and milk products — Determination
of nitrate and nitrite contents:
— Part 1: Method using cadmium reduction and spectrometry
— Part 2: Method using segmented flow analysis (Routine method)
— Part 3: Method using cadmium reduction and flow injection analysis with in-line dialysis (Routine method)
iv © ISO and IDF 2001 – All rights reserved

---------------------- Page: 10 ----------------------

SIST EN ISO 14673-3:2002
ISO 14673-3:2001(E)
IDF 189-3:2001(E)
Foreword
IDF (the International Dairy Federation) is a worldwide federation of the dairy sector with a National Committee in
every member country. Every National Committee has the right to be represented on the IDF Standing Committees
carrying out the technical work. IDF collaborates with ISO and AOAC International in the development of standard
methods of analysis and sampling for milk and milk products.
Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National
Committees for voting. Publication as an International Standard requires approval by at least 50 % of National
Committees casting a vote.
International Standard ISO 14673-3�IDF 189-3 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with
AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International.
All work was carried out by the Joint ISO/IDF/AOAC Action Team, Minerals and minor compounds,of the
Standing Committee on Minor components characterization of physical properties, under the aegis of its project
leader Mr G. Bråthen (NO).
© ISO and IDF 2001 – All rights reserved v

---------------------- Page: 11 ----------------------

SIST EN ISO 14673-3:2002

---------------------- Page: 12 ----------------------

SIST EN ISO 14673-3:2002
ISO 14673-3:2001(E)
INTERNATIONAL STANDARD
IDF 189-3:2001(E)
Milk and milk products — Determination of nitrate and nitrite
contents —
Part 3:
Method using cadmium reduction and flow injection analysis with
in-line dialysis (Routine method)
WARNING — The use of this International Standard may involve hazardous materials, operations and
equipment. This standard does not purport to address all the safety problems associated with its use. It is
the responsibility of the user of this standard to establish safety and health practices and determine the
applicability of regulatory limitations prior to use.
1 Scope
This part of ISO 14673�IDF 189 specifies a routine method for the determination of the nitrate and nitrite contents
of milk and milk products by cadmium reduction and flow injection analysis (FIA). The method is applicable to hard,
semi-hard and soft cheeses of various ages, and processed cheese. The detection limits of the method are 0,5 mg
of nitrate ions per kilogram and 1,0 mg of nitrite ions per kilogram, respectively.
The method is also applicable to whey powder, milk powder and milk-based infant food.
NOTE 1 The method closely resembles the FIA method described in reference [2] for the determination of nitrate and nitrite
in milk and fluid dairy products. Adaptations were made to allow for the analysis of cheese and to obtain sufficient sensitivity for
the determination of low levels of nitrite in cheese and milk-based infant foods.
NOTE 2 For determination of nitrite and nitrate following cadmium reduction, use is made of the same colour reaction as
described in ISO 14673-1�IDF 189-1.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this part of ISO 14673�IDF 189. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this part of ISO 14673�IDF 189 are
encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated
below. For undated references, the latest edition of the normative document referred to applies. Members of ISO
and IEC maintain registers of currently valid International Standards.
ISO 1042, Laboratory glassware — One-mark volumetric flasks
ISO 14673-1�IDF 189-1, Milk and milk products — Determination of nitrate and nitrite contents — Part 1: Method
using cadmium reduction and spectrometry
3 Terms and definitions
For the purposes of this part of ISO 14673�IDF 189, the following terms and definitions apply.
© ISO and IDF 2001 – All rights reserved 1

---------------------- Page: 13 ----------------------

SIST EN ISO 14673-3:2002
ISO 14673-3:2001(E)
IDF 189-3:2001(E)
3.1
nitrate content
mass fraction of nitrate determined by the procedure specified in this part of ISO 14673�IDF 189
–
NOTE The nitrate content is expressed as the mass in milligra
...