Food authenticity - Determination of 16-O-Methylcafestol content of green and roasted coffee - HPLC-method

This document specifies a high-performance liquid chromatography (HPLC) method for determining the 16-O-Methylcafestol content in green and roasted coffee.
The method is suitable for a content of 40 mg/kg to 1 600 mg/kg of 16-O-Methylcafestol of green and roasted coffee, respectively. The collaborative study has shown that mass fractions also between 20 mg/kg to 40 mg/kg can be successfully analysed depending on the laboratory equipment.
The compliance assessment process is not part of this document.

Lebensmittelauthentizität - Bestimmung des Gehaltes an 16‑O‑Methylcafestol in Roh- und Röstkaffee - HPLC-Verfahren

Dieses Dokument legt ein Hochleistungsflüssigchromatographie-(HPLC-)Verfahren zur Bestimmung des Gehaltes an 16-O-Methylcafestol in Roh- und Röstkaffee fest.
Das Verfahren ist für einen Gehalt von 40 mg/kg bis 1 600 mg/kg 16-O-Methylcafestol in Roh- bzw. Röstkaffee geeignet. Der Ringversuch hat gezeigt, dass je nach Laborausrüstung auch Massenanteile zwischen 20 mg/kg und 40 mg/kg erfolgreich analysiert werden können.
Der Prozess der Konformitätsbewertung ist nicht Teil dieses Dokuments.

Authenticité des aliments - Détermination de la teneur en 16-O-méthylcafestol du café vert et torréfié - Méthode CLHP

Le présent document spécifie une méthode de chromatographie en phase liquide à haute performance (CLHP) permettant de déterminer la teneur en 16-O-méthylcafestol dans le café vert et le café torréfié.
La méthode est adaptée pour quantifier une teneur en 16-O-méthylcafestol comprise entre 40 mg/kg et 1 600 mg/kg pour le café vert et le café torréfié respectivement. L’étude interlaboratoires a montré que la méthode permet aussi de quantifier des fractions massiques comprises entre 20 mg/kg et 40 mg/kg, en fonction de l’équipement de laboratoire.
Le présent document ne couvre pas le processus d’évaluation de la conformité.

Pristnost živil - Določanje vsebnosti 16-O-metilkafestola v zeleni in praženi kavi - Metoda HPLC

General Information

Status
Not Published
Publication Date
25-Sep-2024
Current Stage
5020 - Submission to Vote - Formal Approval
Start Date
06-Jun-2024
Due Date
14-Nov-2024
Completion Date
06-Jun-2024

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prEN 18003:2023
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Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN 18003:2023
01-december-2023
Pristnost živil - Določanje vsebnosti 16-O-metilkafestola v zeleni in praženi kavi -
Metoda HPLC
Food Authenticity — Determination of 16-O-methylcafestol content of green and roasted
coffee — HPLC-method
Lebensmittelauthentizität - Bestimmung des Gehaltes an 16-O-Methylcafestol in Roh-
und Röstkaffee - HPLC-Verfahren
Authenticité des aliments - Détermination de la teneur en 16-O-méthylcafestol du café
vert et torréfié - Méthode CLHP
Ta slovenski standard je istoveten z: prEN 18003
ICS:
67.140.20 Kava in kavni nadomestki Coffee and coffee substitutes
oSIST prEN 18003:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 18003:2023

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oSIST prEN 18003:2023


DRAFT
EUROPEAN STANDARD
prEN 18003
NORME EUROPÉENNE

EUROPÄISCHE NORM

August 2023
ICS
English Version

Food Authenticity - Determination of 16-O-methylcafestol
content of green and roasted coffee - HPLC-method
Authenticité alimentaire du café et des produits á base Lebensmittelauthentizität - Bestimmung des Gehaltes
de café ? Détermination de la teneur en 16-O- an 16-O-Methylcafestol in Roh- und Röstkaffee - HPLC-
méthylcafestol dans le café vert et le café torréfié ? Verfahren
Méthode par HPLC
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 460.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 18003:2023 E
worldwide for CEN national Members.

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oSIST prEN 18003:2023
prEN 18003:2023 (E)
Contents Page
European foreword . Error! Bookmark not defined.
Introduction . Error! Bookmark not defined.
8.1 General. Error! Bookmark not defined.
8.2 Sample preparation of roasted coffee . Error! Bookmark not defined.
8.3 Sample preparation of green coffee . Error! Bookmark not defined.
8.4 Isolation of the unsaponifiable matter. Error! Bookmark not defined.
9.1 General. Error! Bookmark not defined.
9.2 Calculation . Error! Bookmark not defined.
Annex A (informative) Typical HPLC chromatograms . Error! Bookmark not defined.
Annex B (informative) Statistical parameters and precision data . Error! Bookmark not defined.
Annex C (informative) Common collaborative study of the HPLC and NMR methods
(prEN XXXX:2023 and prEN 17992:2023) regarding roasted coffeesError! Bookmark not defined.
C.1 Background of the collaborative study . Error! Bookmark not defined.
C.2 Explanati
...

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