EN ISO 12966-4:2015

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4: Determination by capillary gas chromatography (ISO 12966-4:2015)

ISO 12966-4:2015 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.
The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources.
This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966.
ISO 12966-4:2015 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.

Tierische und pflanzliche Fette und Öle - Gaschromatographie von Fettsäuremethylestern - Teil 4: Bestimmung mittels Kapillargaschromatographie (ISO 12966-4:2015)

Dieser Teil der ISO 12966 legt ein Verfahren zur Bestimmung von Fettsäuremethylestern (FAME), die durch Umesterung oder Veresterung von Fetten, Ölen und Fettsäuren gewonnen wurden, mittels Kapillar-gaschromatographie (GLC) fest. Durch die Anwendung dieses Teiles von ISO 12966 können Fett-säuremethylester von C8 bis C24, einschließlich gesättigte Fettsäuremethylester, einfach ungesättigte cis  und trans Fettsäuremethylester sowie mehrfach ungesättigte cis  und trans-Fettsäure¬methylester abgetrennt werden.
Das Verfahren ist geeignet für rohe, raffinierte, teilweise oder vollständig hydrierte Fette, Öle und Fettsäuren, die aus tierischen und pflanzlichen Quellen gewonnen wurden.
Dieses Verfahren ist nicht geeignet für die Untersuchung von Milch, Fetten und Ölen von Wiederkäuern, oder Produkten ergänzt mit konjugierter Linolsäure (CLA). Milch und Milcherzeugnisse (oder Fett aus Milch und Milcherzeugnissen) sind vom Anwendungsbereich dieses Teiles der ISO 12966 ausgeschlossen.
Dieser Teil der ISO 12966 ist nicht geeignet für di-, tri-, polymerisierte und oxidierte Fettsäuren und Fette und Öle.

Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des esters méthyliques d'acides gras - Partie 4: Détermination par chromatographie capillaire en phase gazeuse (ISO 12966-4:2015)

ISO 12966-4:2015 spécifie une méthode de détermination des esters méthyliques d'acides gras (EMAG) obtenus par transestérification ou estérification de corps gras et d'acide gras par chromatographie en phase gazeuse (CPG) sur colonne capillaire. Les esters méthyliques d'acides gras de C8 à C24 peuvent être séparés en utilisant la présente partie de l'ISO 12966, y compris les esters méthyliques d'acides gras saturés, les esters méthyliques d'acides gras mono-insaturés cis et trans et les esters méthyliques d'acides gras poly-insaturés cis et trans.
La méthode s'applique aux corps gras et acides gras bruts, raffinés, partiellement hydrogénés ou totalement hydrogénés d'origines animale et végétale.
La présente méthode ne convient pas à l'analyse des matières grasses laitières provenant des ruminants, ou des produits supplémentés en acide linoléique conjugué (CLA). Le lait et les produits laitiers (ou corps gras dérivés du lait et des produits laitiers) ne relèvent pas du domaine d'application de la présente partie de l'ISO 12966.
ISO 12966-4:2015 ne s'applique pas aux acides gras et corps gras dimérisés, trimérisés, polymérisés et oxydés.

Živalske in rastlinske maščobe in olja - Plinska kromatografija metilnih estrov maščobnih kislin - 4. del: Določevanje s kapilarno plinsko kromatografsko metodo (ISO 12966-4:2015)

Ta del standarda ISO 12966 določa metodo za določanje metilnih estrov maščobnih kislin (FAME), pridobljenih s transesterifikacijo ali esterifikacijo iz maščob, olj in maščobnih kislin, s kapilarno plinsko kromatografsko metodo. Metilne estre maščobnih kislin C8 do C24 je mogoče ločiti z uporabo metode iz tega dela standarda ISO 12966, vključno z metilnimi estri nasičenih maščobnih kislin, metilnimi estri cis- in trans-enkratnenasičenih maščobnih kislin in metilnimi estri cis- in trans-večkratnenasičenih maščobnih kislin.
Ta metoda velja za surove, rafinirane in delno ali povsem hidrogenirane maščobe, olja in maščobne kisline, pridobljene iz živalskih in rastlinskih virov. Ta metoda ni primerna za analizo mlečnih izdelkov, maščob in olj prežvekovalcev ali izdelkov z dodano konjugirano linolno kislino (CLA). Mleko in mlečni izdelki (ali maščoba iz mleka in mlečnih izdelkov) niso zajeti v tem delu standarda ISO 12966. Ta del standarda ISO 12966 ne velja za dimerne, trimerne, polimerne in oksidirane maščobne kisline.

General Information

• Status: Published
• Publication Date: 09-Jun-2015
• Withdrawal Date: 30-Dec-2015
• ICS: 67.200.10 - Animal and vegetable fats and oils
• ICS: 67.220.10 - Spices and condiments
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 10-Jun-2015
• Completion Date: 10-Jun-2015
• Ref Project: SIST EN ISO 12966-4:2015 - Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4: Determination by capillary gas chromatography (ISO 12966-4:2015)

Relations

• Replaces: EN ISO 5508:1995 - Animal and vegetable fats and oils - Analysis by gas chromatography of methyl esters of fatty acids (ISO 5508:1990)
• Effective Date: 08-Jun-2022
• Replaces: EN ISO 15304:2002/AC:2004 - Animal and vegetable fats and oils - Determination of the content of trans fatty acid isomers of vegetable fats and oils - Gas chromatographic method (ISO 15304:2002/Cor.1:2003)
• Effective Date: 24-Jun-2015
• Replaces: EN ISO 15304:2002 - Animal and vegetable fats and oils - Determination of the content of trans fatty acid isomers of vegetable fats and oils - Gas chromatographic method (ISO 15304:2002)
• Effective Date: 24-Jun-2015

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 12966-4:2015
01-september-2015
1DGRPHãþD
SIST EN ISO 15304:2002
SIST EN ISO 15304:2002/AC:2005
SIST EN ISO 5508:1996
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD3OLQVNDNURPDWRJUDILMDPHWLOQLKHVWURY
PDãþREQLKNLVOLQGHO'RORþHYDQMHVNDSLODUQRSOLQVNRNURPDWRJUDIVNRPHWRGR
,62
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters -
Part 4: Determination by capillary gas chromatography (ISO 12966-4:2015)
Tierische und pflanzliche Fette und Öle - Gaschromatographie von
Fettsäuremethylestern - Teil 4: Bestimmung von cis-, trans-, gesättigten, mono- und
mehrfach ungesättigten Fettsäuren in pflanzlichen oder nicht-ruminanten Ölen und
Fetten (ISO 12966-4:2015)
Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des
esters méthyliques d'acides gras - Partie 4: Détermination des acides gras saturés,
mono- et poly-insaturés, cis ou trans, dans les corps gras d'origines végétale ou animale
(non ruminant)
Ta slovenski standard je istoveten z: EN ISO 12966-4:2015
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
SIST EN ISO 12966-4:2015 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 12966-4:2015

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SIST EN ISO 12966-4:2015

EUROPEAN STANDARD
EN ISO 12966-4

NORME EUROPÉENNE

EUROPÄISCHE NORM
June 2015
ICS 67.200.10 Supersedes EN ISO 15304:2002, EN ISO 5508:1995
English Version
Animal and vegetable fats and oils - Gas chromatography of fatty
acid methyl esters - Part 4: Determination by capillary gas
chromatography (ISO 12966-4:2015)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle -
Chromatographie en phase gazeuse des esters Gaschromatographie von Fettsäuremethylestern - Teil 4:
méthyliques d'acides gras - Partie 4: Détermination par Bestimmung mittels Kapillargaschromatographie (ISO
chromatographie capillaire en phase gazeuse (ISO 12966- 12966-4:2015)
4:2015)
This European Standard was approved by CEN on 16 April 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12966-4:2015 E
worldwide for CEN national Members.

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SIST EN ISO 12966-4:2015
EN ISO 12966-4:2015 (E)
Contents Page
Foreword .3

2

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SIST EN ISO 12966-4:2015
EN ISO 12966-4:2015 (E)
Foreword
This document (EN ISO 12966-4:2015) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and
oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by December 2015, and conflicting national standards shall be withdrawn
at the latest by December 2015.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 5508:1995, EN ISO 15304:200
...