Sludge, treated biowaste and soil - Determination of viable plant seeds and propagules

This Technical Specification specifies a test procedure for the determination of the content of unwanted viable weed seeds and plant propagules in growing media and soil improvers (see also Annex B for validation results). The method in general is also applicable to soils and sludges.

Schlamm, behandelter Bioabfall und Boden - Bestimmung der Keimfähigkeit von wachstumsfähigen Grassamen und Pflanzenstecklingen

Diese Technische Spezifikation legt ein Prüfverfahren zur Bestimmung der Anzahl keimfähiger Unkrautsamen und Pflanzenkeimlinge in Kultursubstraten und Bodenverbesserungsmitteln fest (für Ergebnisse der Validierung siehe Anhang B).
Das Verfahren ist allgemein auch auf Böden und Schlämme anwendbar.

Boues, bio-déchets traités et sols - Détermination de la germination des graines adventices viables et des propagules végétales

La présente Spécification technique prescrit un mode opératoire d'essai en vue de la détermination de la teneur en graines adventices viables et propagules végétales indésirables dans des supports de culture et des amendements du sol (voir aussi l’Annexe B pour les résultats de validation).
En général, cette méthode est également applicable aux sols et aux boues.

Blato, obdelani biološki odpadki in tla - Določevanje kalivosti rastlinskih semen in preživetvene sposobnosti vegetativnih razmnoževalnih organov

Ta tehnična specifikacija določa preskusni postopek za določevanje vsebnosti neželenih semen plevela in rastlinskih propagul v rastnih substratih in izboljševalcih tal (glej tudi dodatek B za rezultate validacije). Ta metoda v splošnem velja tudi za tla in blato.

General Information

Status
Published
Public Enquiry End Date
31-Jan-2011
Publication Date
21-Nov-2013
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
07-Nov-2013
Due Date
12-Jan-2014
Completion Date
22-Nov-2013

Buy Standard

Standard + National Annex and/or Foreword
SIST-TS CEN/TS 16201:2013
Foreword and/or annex in Slovenian language, body of the standard in English language
14 pages
sale 10% off
Preview
sale 10% off
Preview

e-Library read for
1 day

Standards Content (sample)

SIST-TS CEN/TS 16201
SLOVENSKA
TEHNIČNA
december 2013
SPECIFIKACIJA
Blato, obdelani biološki odpadki in tla – Določevanje kalivosti rastlinskih
semen in preživetvene sposobnosti vegetativnih razmnoževalnih organov
Sludge, treated biowaste and soil – Determination of viable plant seeds and
propagules

Boues, bio-déchets traités et sols – Détermination de la germination des graines

adventices viables et des propagules végétales
Schlamm, behandelter Bioabfall und Boden – Bestimmung der Keimfähigkeit von
wachstumsfähigen Grassamen und Pflanzenstecklingen
Referenčna oznaka
ICS 13.030.20; 13.080.30 SIST-TS CEN/TS 16201:2013 ((sl),en)
Nadaljevanje na straneh II do III in od 1 do 12

© 2013-12. Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST-TS CEN/TS 16201 : 2013
NACIONALNI UVOD

Tehnična specifikacija SIST-TS CEN/TS 16201 ((sl),en), Blato, obdelani biološki odpadki in tla –

Določevanje kalivosti rastlinskih semen in preživetvene sposobnosti vegetativnih razmnoževalnih

organov, 2013, ima status slovenske tehnične specifikacije in je istovetna evropski tehnični

specifikaciji CEN/TS 16201, Sludge, treated biowaste and soil – Determination of viable plant seeds

and propagules, 2013.
NACIONALNI PREDGOVOR

Evropsko tehnično specifikacijo CEN/TS 16201:2013 je pripravil tehnični odbor Evropskega komiteja

za standardizacijo CEN/TC 400 Horizontalni standardi na področju blata, obdelanih bioloških

odpadkov in tal, katerega sekretariat vodi DIN.

CEN je pripravil ta dokument na podlagi mandata M/330 Evropske komisije in Evropskega združenja

za prosto trgovino, ki narekuje pripravo standardov za vzorčenje in analizne metode za higienske in

biološke kakor tudi za anorganske in organske parametre, ki bi bili primerni za blata, obdelane

biološke odpadke in tla, kolikor je to tehnično izvedljivo.

Odločitev za privzem te tehnične specifikacije je dne 25. septembra 2013 sprejel tehnični odbor

SIST/TC KAT Kakovost tal.
ZVEZE S STANDARDI

S privzemom te evropske tehnične specifikacije veljajo za omenjeni namen referenčnih standardov vsi

standardi, navedeni v izvirniku, razen tistih, ki so že sprejeti v nacionalno standardizacijo:

SIST EN 13037 (en) Izboljševalci tal in rastni substrati – Določevanje pH

SIST EN 13038 (en) Izboljševalci tal in rastni substrati – Določevanje električne

prevodnosti

SIST EN 13040 (en) Izboljševalci tal in rastni substrati – Priprava vzorcev za kemijske in

fizikalne preskuse, določevanje suhe snovi, vlage in laboratorijsko
stisnjene prostorninske gostote
SIST EN 15933 (en) Blato, obdelani biološki odpadki in tla – Določevanje pH

SIST-TS CEN/TS 15937 (en) Blato, obdelani biološki odpadki in tla – Določevanje specifične

električne prevodnosti

SIST EN ISO 3696 (en) Voda za analitsko laboratorijsko uporabo – Specifikacija in preskusne

metode (ISO 3696)
IZRAZI IN DEFINICIJE
V tem dokumentu se uporabljajo naslednji izrazi in definicije:
3.1

vegetativni razmnoževalni organi (angl. plant propagule; nem. Pflanzenfortpflanzungskeim)

del rastline, ki je sposoben odgnati
3.2
plevel (angl. weed; nem. Unkraut)
nezaželene kaleče ali vznikle rastline
OSNOVA ZA IZDAJO TEHNIČNE SPECIFIKACIJE
– privzem tehnične specifikacije CEN/TS 16201:2013
---------------------- Page: 2 ----------------------
SIST-TS CEN/TS 16201 : 2013
OPOMBE

– Povsod, kjer se v besedilu tehnične specifikacije uporablja izraz “evropska tehnična

specifikacija”, v SIST-TS CEN/TS 16201:2013 to pomeni “slovenska tehnična specifikacija”.

– Nacionalni uvod in nacionalni predgovor nista sestavni del te tehnične specifikacije.

– Ta nacionalni dokument je istoveten s CEN/TS 16201:2013 in je objavljen z dovoljenjem

CEN
Management Centre
Avenue Marnix 17
B-1000 Brussels

This national document is identical with CEN/TS 16201:2013 and is published with the permission of

CEN
Management Centre
Avenue Marnix 17
B-1000 Brussels
III
---------------------- Page: 3 ----------------------
TECHNICAL SPECIFICATION
CEN/TS 16201
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
October 2013
ICS 13.030.01
English Version
Sludge, treated biowaste and soil - Determination of viable plant
seeds and propagules

Boues, bio-déchets traités et sols - Détermination de la Schlamm, behandelter Bioabfall und Boden - Bestimmung

germination des graines adventices viables et des keimfähiger Pflanzensamen und Keimlinge

propagules végétales

This Technical Specification (CEN/TS) was approved by CEN on 5 March 2011 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their

comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available

promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)

until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United

Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 16201:2013: E

worldwide for CEN national Members.
---------------------- Page: 4 ----------------------
CEN/TS 16201:2013 (E)
Contents Page

Foreword ....................................................................................................................................................... 3

Introduction .................................................................................................................................................. 4

1 Scope ................................................................................................................................................ 5

2 Normative references ....................................................................................................................... 5

3 Terms and definitions ...................................................................................................................... 5

4 Principle............................................................................................................................................ 5

5 Reagents and test materials ............................................................................................................ 5

6 Apparatus ......................................................................................................................................... 6

7 Preparation of the sample ................................................................................................................ 6

7.1 General preparation ......................................................................................................................... 6

7.2 Dilution ............................................................................................................................................. 6

7.3 Moistening ........................................................................................................................................ 7

8 Procedure ......................................................................................................................................... 7

8.1 Experimental design ........................................................................................................................ 7

8.2 Calculation and expression of results ............................................................................................ 7

9 Precision ........................................................................................................................................... 8

10 Test report ........................................................................................................................................ 8

Annex A (informative) Additional pretreatment methods ............................................................................ 9

A.1 Pretreatment to break seed dormancy ............................................................................................ 9

A.1.1 Stratification ..................................................................................................................................... 9

A.1.2 Treatment with gibberellic acid (GA) ............................................................................................... 9

A.1.3 Treatment with potassium nitrate (KNO ) ....................................................................................... 9

A.2 Leaching procedure ......................................................................................................................... 9

A.3 Fist test ........................................................................................................................................... 10

Annex B (informative) Repeatability and reproducibility data................................................................... 11

B.1 Materials used in the interlaboratory comparison study.............................................................. 11

B.2 Interlaboratory comparison results ............................................................................................... 11

Bibliography................................................................................................................................................ 12

---------------------- Page: 5 ----------------------
CEN/TS 16201:2013 (E)
Foreword

This document (CEN/TS 16201:2013) has been prepared by Technical Committee CEN/TC 400 “Project

Committee - Horizontal standards in the field of sludge, biowaste and soil”, the secretariat of which is held by

DIN.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

This document has been prepared under a mandate given to CEN by the European Commission and the

European Free Trade Association.

This Technical Specification is part of a modular horizontal approach in which this document belongs to the

analytical step.

The preparation of this document by CEN is based on a mandate by the European Commission (Mandate

M/330), which assigned the development of standards on sampling and analytical methods for hygienic and

biological parameters as well as inorganic and organic determinants, aiming to make these standards

applicable to sludge, treated biowaste and soil as far as this is technically feasible.

According to the CEN-CENELEC Internal Regulations, the national standards organisations of the following

countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Croatia, Cyprus,

Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany,

Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,

Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.

---------------------- Page: 6 ----------------------
CEN/TS 16201:2013 (E)
Introduction

This Technical Specification is the result of a desk study "Horizontal European Standards for Contamination

with Viable Weed Seeds and Plant Propagules" which aimed at evaluating the latest developments in

assessing weeds and plant propagules in sludge, treated biowaste and soil.
---------------------- Page: 7 ----------------------
CEN/TS 16201:2013 (E)
1 Scope

This Technical Specification specifies a test procedure for the determination of the content of unwanted viable

weed seeds and plant propagules in growing media and soil improvers (see also Annex B for validation

results).
The method in general is also applicable to soils and sludges.
2 Normative references

The following documents, in whole or in part, are normatively referenced in this document and are

indispensable for its application. For dated references, only the edition cited applies. For undated references,

the latest edition of the referenced document (including any amendments) applies.

EN 13037, Soil improvers and growing media — Determination of pH

EN 13038, Soil improvers and growing media — Determination of electrical conductivity

EN 13040, Soil improvers and growing media — Sample preparation for chemical and physical tests,

determination of dry matter content, moisture content and laboratory compacted bulk density

EN 15933, Sludge, treated biowaste and soil — Determination of pH

CEN/TS 15937, Sludge, treated biowaste and soil — Determination of specific electrical conductivity

EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696)

3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
plant propagule
part of a plant capable of tillering
3.2
weed
any unwanted plant that germinates or emerges
4 Principle

Samples are gently pretreated (by sieving, mixing, sub-dividing), the pH and the electrical conductivity are

measured, and the development of plants, whether from seeds, or plant propagules, is determined after a

21-day incubation period under controlled conditions.
5 Reagents and test materials
5.1 Water, quality 3 according to EN ISO 3696:1995 (tap water).

5.2 Sphagnum peat, with a degree of humification of H3 to H5, according to von Post scale,

...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.