Refers to taper dimensions and tolerances for electrode caps, electrode adaptors, electrode holders and similar parts, where the electrode force Fmax, given for diameter d1 in tables 1, 2 and 3 is not exceeded. Establishes dimensions, designation and marking. Cancels and replaces ISO Recommendation R 1089-1969, of which it constitutes a technical revision.

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This document specifies acceptable criteria for microbiological cleanliness and adequacy of preservation of the specified toy materials. The requirements in this document apply to all toys that are, contain or are supplied with aqueous materials (e.g. paste, putty, liquid or gel). In addition, this document applies to toys that are or include a cosmetic (including those intended for use on a toy as well as on the child). Powders and similar substances intended to be mixed with water are also within the scope of this document. The cleanliness and preservation effectiveness requirements are applicable to a toy as it is initially received by the consumer in an unopened and undamaged container and do not apply after a toy is subjected to reasonably foreseeable conditions of normal use and abuse, unless specifically noted otherwise. The microbial limits and test methods contained in this document are inappropriate to apply to products that are consumer complaint returns, as there is no way to establish what conditions the toys have been subject to before being returned. The following are excluded from the scope of this document: — materials that are inaccessible during normal use or reasonably foreseeable abuse; — powder or powder-like materials intended to show biological phenomena, e.g. shrimp eggs, seeds, soil; — food.

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This document specifies general criteria to be applied in the determination of bacterial endotoxins on or in health care products, components or raw materials using bacterial endotoxins test (BET) methods, using amebocyte lysate reagents. This document is not applicable to the evaluation of pyrogens other than bacterial endotoxins. Other endotoxin detection methodologies are not included (see B.12). This document does not address setting specific endotoxin limit specifications.

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This document specifies a method for determining the bacteria, yeast and mould population on the surface of paper and paperboard. The enumeration relates to specific media. This document is applicable to all kinds of paper and paperboard, to dry market pulp in sheet form and to packaging material.

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This International Standard specifies requirements for the evaluation of membrane filters intended for the concentration for direct enumeration of specific microorganisms and mixed populations. These requirements are applicable to all membrane filters intended for the microbiological analysis of all kinds of water and other liquid samples.
These requirements are intended for the test to demonstrate the suitability of the whole system - membrane filter together with culture medium including the filtration step - required for specific tests described in International Standards.
The membrane filters required for use are described in each specific standard. This International Standard applies to producers and users such as:
— commercial bodies producing and/or distributing membrane filters;
— non-commercial bodies supplying membrane filters to third parties;
— microbiological laboratories using membrane filters for their own testing or providing these to other users.

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This method describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in
chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut
detected from the gene for corA 1.

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Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media

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This document specifies the detection of Clostridium perfringens. This part of ISO 15213 is applicable to:
• products intended for human consumption;
• products intended for animal feeding;
• environmental samples in the area of food and feed production, handling, and
• samples from the primary production stage.
This method is applicable when the number sought is expected to be below 100 per ml or per g of the test sample.

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Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media

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This method specifies a procedure for the qualitative detection of species specific DNA from
white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex realtime
PCR based on the genes MADS-D (mustard) and lectin (soya). A mustard content of 10
mg/kg or greater and a soya content of 10 mg/kg or greater can be detected with a probability of
> 95 %.

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This method describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in
chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [4, 5].

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This document specifies a method for the detection of hazelnut (Corylus avellana) in chocolate.
Real-time PCR (Polymerase chain reaction) detection of hazelnut is based on an152 bp (base pair) sequence from the corA 1 gene of hazelnut.

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This document specifies a procedure for the qualitative detection of species specific DNA from white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex real-time PCR based on the genes MADS-D (mustard) and lectin (soya).

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This document specifies a method for the detection of peanut (Arachis hypogaea) in chocolate.
Real-time PCR (Polymerase Chain Reaction) detection of peanut is based on an 86 bp (base pair) sequence from the Ara h 2 gene of peanut.

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Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media

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This document specifies the enumeration of sulfite-reducing Clostridium spp. by the colony-count technique.
This document is applicable to:
—    products intended for human consumption;
—    products for feeding animals;
—    environmental samples in the area of food and feed production and handling;
—    samples from the primary production stage.
NOTE      This method has been validated in an interlaboratory study for the following food categories:
—    ready-to-eat, ready-to-reheat meat products;
—    eggs and egg products (derivates);
—    processed fruits and vegetables;
—    infant formula and infant cereals;
—    multi-component foods or meal components.
It has also been validated for the following other categories:
—    pet food and animal feed;
—    environmental samples (food or feed production).
As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category.
This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.

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This document specifies the requirements for the performance testing of membrane filters used for the retention followed by direct enumeration of microorganisms by culture methods.
This document is applicable to membrane filters which are used for retention followed by direct enumeration of specific microorganisms on solid media or on other devices containing media, like absorbent pads[19].
This document is not applicable for membrane filters used for concentration and elution or for qualitative methods.
These tests are applicable to the membrane filters intended for the microbiological analysis of different types of water, such as:
—    drinking water, bottled water and other types of water with expected low numbers of microorganisms;
—    water with expected higher numbers of microorganisms, for example, surface water and process water.
These tests are intended to demonstrate the suitability of the whole system (membrane filter together with the culture medium including the filtration step) required for the specific tests described in References [3], [6], [8], [10], [12] and [13].
This document applies to:
—    manufacturers producing membrane filters;
—    microbiological laboratories using membrane filters for their own testing or providing these to other end users.

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This document specifies the enumeration of sulfite-reducing Clostridium spp. by the colony-count technique. This document is applicable to: — products intended for human consumption; — products for feeding animals; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. NOTE This method has been validated in an interlaboratory study for the following food categories: — ready-to-eat, ready-to-reheat meat products; — eggs and egg products (derivates); — processed fruits and vegetables; — infant formula and infant cereals; — multi-component foods or meal components. It has also been validated for the following other categories: — pet food and animal feed; — environmental samples (food or feed production). As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.

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This document specifies the requirements for the performance testing of membrane filters used for the retention followed by direct enumeration of microorganisms by culture methods. This document is applicable to membrane filters which are used for retention followed by direct enumeration of specific microorganisms on solid media or on other devices containing media, like absorbent pads[19]. This document is not applicable for membrane filters used for concentration and elution or for qualitative methods. These tests are applicable to the membrane filters intended for the microbiological analysis of different types of water, such as: — drinking water, bottled water and other types of water with expected low numbers of microorganisms; — water with expected higher numbers of microorganisms, for example, surface water and process water. These tests are intended to demonstrate the suitability of the whole system (membrane filter together with the culture medium including the filtration step) required for the specific tests described in References [3], [6], [8], [10], [12] and [13]. This document applies to: — manufacturers producing membrane filters; — microbiological laboratories using membrane filters for their own testing or providing these to other end users.

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This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
—    products intended for human consumption;
—    products intended for animal feed;
—    environmental samples from food and feed handling and production areas;
—    samples from the primary production stage.

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This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products.
The use of this document can be extended to yeasts that do not form mycelium.

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This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products.
The use of this document can be extended to yeasts that do not form mycelium.

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This document specifies the protocols for conducting microbiological challenge tests for inactivation studies on vegetative bacteria and bacterial spores in the raw materials and ingredients, intermediate or end products. The use of this document can be extended to yeasts which do not form mycelium.

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