EN ISO 11290-2:2017

Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method (ISO 11290-2:2017)

ISO 11290-2:2017 specifies a horizontal method for
-      the enumeration of L. monocytogenes, and
-      the enumeration of Listeria spp. (including L. monocytogenes).
ISO 11290-2:2017 is applicable to
-      products intended for human consumption and for the feeding of animals, and
-      environmental samples in the area of food production and food handling.

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für den Nachweis und die Zählung von Listeria monocytogenes und von Listeria spp. - Teil 2: Zählverfahren (ISO 11290-2:2017)

Dieser Teil von ISO 11290 legt ein horizontales Verfahren fest für:
—   die Zählung von L. monocytogenes;
—   die Zählung von Listeria spp. (einschließlich L. monocytogenes).
Dieser Teil von ISO 11290 gilt für:
—   Produkte, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind;
—   Umgebungsproben im Bereich der Herstellung von Lebensmitteln und beim Umgang mit Lebensmitteln.
Bestimmte zusätzlich beschriebene Listeria Arten sind mit diesem Verfahren möglicherweise nicht zählbar oder bestätigbar (siehe Literaturhinweise [3], [6], [9], [11]).

Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le dénombrement de Listeria monocytogenes et de Listeria spp. - Partie 2: Méthode de dénombrement (ISO 11290-2:2017)

ISO 11290-2:2017 spécifie une méthode horizontale pour:
-      le dénombrement de L. monocytogenes; et
-      le dénombrement de Listeria spp. (y compris L. monocytogenes).
ISO 11290-2:2017 s'applique aux:
-      produits destinés à la consommation humaine ou à l'alimentation animale; et
-      échantillons de l'environnement de production et de distribution des aliments.

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp. - 2. del: Metoda štetja (ISO 11290-2:2017)

Ta standard opisuje način ugotavljanja števila Listeria monocytogenes (referenčni dokument: EN/ISO 11290 -2 vklj./dod. 1)

General Information

• Status: Published
• Publication Date: 13-Jun-2017
• Withdrawal Date: 30-Dec-2017
• ICS: 07.100 - Microbiology
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 14-Jun-2017
• Due Date: 01-Mar-2014
• Completion Date: 14-Jun-2017
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Mandate: M/381 - Mandate for standardisation addressed to CEN in the field of Methods of analysis of foodstuffs concerning Food Hygiene
• Ref Project: SIST EN ISO 11290-2:2017 - Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method (ISO 11290-2:2017)

Relations

• Replaces: EN ISO 11290-2:1998/A1:2004 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 2: Enumeration method - Amendment 1: Modification of the enumeration medium (ISO 11290-2:1998/AM1:2004)
• Effective Date: 21-Jun-2017
• Replaces: EN ISO 11290-2:1998 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 2: Enumeration method (ISO 11290-2:1998)
• Effective Date: 21-Jun-2017

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2017
1DGRPHãþD
SIST EN ISO 11290-2:1999
SIST EN ISO 11290-2:1999/A1:2005
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti in števila Listeria monocytogenes in Listeria spp. - 2. del: Metoda štetja
(ISO 11290-2:2017)
Microbiology of the food chain - Horizontal method for the detection and enumeration of
Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method (ISO 11290-
2:2017)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für den Nachweis und die
Zählung von Listeria monocytogenes und von Listeria spp. - Teil 2: Zählverfahren (ISO
11290-2:2017)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le
dénombrement de Listeria monocytogenes et de Listeria spp. - Partie 2: Méthode de
dénombrement (ISO 11290-2:2017)
Ta slovenski standard je istoveten z: EN ISO 11290-2:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 11290-2
EUROPEAN STANDARD
NORME EUROPÉENNE
June 2017
EUROPÄISCHE NORM
ICS 07.100.30 Supersedes EN ISO 11290-2:1998
English Version
Microbiology of the food chain - Horizontal method for the
detection and enumeration of Listeria monocytogenes and
of Listeria spp. - Part 2: Enumeration method (ISO 11290-
2:2017)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour la recherche et le dénombrement de Verfahren für den Nachweis und die Zählung von
Listeria monocytogenes et de Listeria spp. - Partie 2: Listeria monocytogenes und von Listeria spp. - Teil 2:
Méthode de dénombrement (ISO 11290-2:2017) Zählverfahren (ISO 11290-2:2017)
This European Standard was approved by CEN on 6 April 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11290-2:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 11290-2:2017) has been prepared by Technical Committee ISO/TC 34 ” Food
products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by December 2017, and conflicting national standards
shall be withdrawn at the latest by December 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11290-2:1998.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11290-2:2017 has been approved by CEN as EN ISO 11290-2:2017 without any
modification.
INTERNATIONAL ISO
STANDARD 11290-2
Second edition
2017-05
Microbiology of the food chain —
Horizontal method for the detection
and enumeration of Listeria
monocytogenes and of Listeria spp. —
Part 2:
Enumeration method
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche et le dénombrement de Listeria monocytogenes et de
Listeria spp. —
Partie 2: Méthode de dénombrement
Reference number
ISO 11290-2:2017(E)
©
ISO 2017
ISO 11290-2:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
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ii © ISO 2017 – All rights reserved

ISO 11290-2:2017(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 2
4.1 General . 2
4.2 Initial suspension . 2
4.3 Surface plating . 2
4.4 Incubation . 2
4.5 Confirmation . 3
4.6 Enumeration . 3
5 Culture media and reagents . 3
6 Equipment and consumables . 3
7 Sampling . 3
8 Preparation of test sample . 4
9 Procedure. 4
9.1 Test portion, initial suspension and dilutions . 4
9.2 Inoculation and incubation . 4
9.3 Enumeration of characteristic colonies . 4
9.4 Confirmation of L. monocytogenes or Listeria spp. . 5
9.4.1 Selection of colonies for confirmation . 5
9.4.2 Confirmation of L. monocytogenes . 6
9.4.3 Confirmation of Listeria spp. . 9
9.5 Interpretation of morphological and physiological properties and of the
biochemical reactions .10
9.6 Additional characterization of isolated strains (optional) .10
10 Expression of results .10
11 Performance characteristics of the method .10
11.1 Method validation study .10
11.2 Repeatability limit .11
11.3 Reproducibility limit .11
12 Test report .11
13 Quality assurance .12
Annex A (normative) Diagram of procedure .13
Annex B (normative) Composition and preparation of media and reagents .14
Annex C (informative) Distinction of Listeria spp. from other genera .22
Annex D (informative) Reactions for the identification of Listeria species .23
Annex E (informative) Results of interlaboratory studies for enumeration of
Listeria monocytogenes .25
Bibliography .28
ISO 11290-2:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national
...