EN ISO 20418-3:2020

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020)

This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction.
NOTE 1    The composition of non-animal hair fibres can be measured by ISO 1833 (all parts). Both results are combined to determine the total fibre composition.
The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827[4]. It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present.
NOTE 2    In this case, the quantitative analysis is performed using microscopical analysis [for example, ISO 17751 (all parts)].

Textilien - Qualitative und quantitative Proteomanalyse einiger Tierhaarfasern - Teil 3: Peptiddetektion mit LC-MS ohne Proteinreduktion (ISO 20418-3:2020)

Dieses Dokument legt ein qualitatives und quantitatives Verfahren zur Bestimmung der Zusammensetzung von Tierhaarfasermischungen (aus Wolle, Kaschmir, Yak, Alpaka, Kamel oder Angora) mittels LC MS ohne Proteinreduktion fest.
ANMERKUNG 1 Die Zusammensetzung von Haarfasern nichttierischen Ursprungs kann mithilfe von ISO 1833 (alle Teile) bestimmt werden. Beide Ergebnisse werden kombiniert, um die Gesamtfaserzusammensetzung zu bestimmen.
Das Verfahren beruht auf einer Vorabidentifizierung, mittels Lichtmikroskopie, aller Fasern in der Mischung auf der Grundlage ihrer Morphologie nach ISO/TR 11827 [4]. Es ist nicht anzuwenden, wenn Fasern von der gleichen Tierart vorliegen (wie zum Beispiel Mischungen aus Kaschmir und Mohair).
ANMERKUNG 2 In diesem Fall wird die quantitative Analyse mittels mikroskopischer Analyse durchgeführt [zum Beispiel ISO 17751 (alle Teile)].

Textiles - Analyse protéomique qualitative et quantitative de certaines fibres animales - Partie 3: Détection des peptides par LC-MS sans réduction protéique (ISO 20418-3:2020)

Le présent document spécifie une méthode qualitative et quantitative pour déterminer la composition des mélanges de fibres animales ? poils - (composés de laine, de cachemire, de yack, d'alpaga, de chameau ou d'angora) par LC-MS sans réduction protéique.
NOTE 1    La composition des fibres autres que les poils animaux  peut être mesurée conformément à l'ISO 1833 (toutes les parties). Les résultats de ces deux méthodes sont ensuite combinés pour déterminer la composition globale des fibres.
La méthode repose sur une identification préliminaire, par microscopie optique, de toutes les fibres présentes dans le mélange sur la base de leur morphologie, conformément à l'ISO/TR 11827[4]. Elle n'est pas applicable si des fibres de la même espèce animale (telles que des mélanges de cachemire et de mohair) sont présentes.
NOTE 2    Dans ce cas, l'analyse quantitative est réalisée par microscopie [tel que décrit dans l'ISO 17751 (toutes les parties), par exemple].

Tekstilije - Kvalitativna in kvantitativna proteomska analiza nekaterih živalskih vlaken - 3. del: Odkrivanje peptida z uporabo LC-MS brez zmanjšanja proteina (ISO 20418-3:2020)

General Information

• Status: Published
• Publication Date: 30-Jun-2020
• Withdrawal Date: 30-Jan-2021
• ICS: 59.060.01 - Textile fibres in general
• Technical Committee: CEN/TC 248 - Textiles and textile products
• Drafting Committee: CEN/TC 248/WG 30 - Quantitative analysis of fibre mixtures
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 01-Jul-2020
• Completion Date: 01-Jul-2020
• Ref Project: SIST EN ISO 20418-3:2020 - Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 20418-3:2020
01-september-2020
Tekstilije - Kvalitativna in kvantitativna proteomska analiza nekaterih živalskih
vlaken - 3. del: Odkrivanje peptida z uporabo LC-MS brez zmanjšanja proteina (ISO
20418-3:2020)
Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres -
Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020)
Textilien - Qualitative und quantitative Proteomanalyse einiger Tierhaarfasern - Teil 3:
Peptiddetektion mit LC-MS ohne Proteinreduktion (ISO 20418-3:2020)
Textiles - Analyse protéomique qualitative et quantitative de certaines fibres animales -
Partie 3: Détection des peptides par LC-MS sans réduction protéique (ISO 20418-
3:2020)
Ta slovenski standard je istoveten z: EN ISO 20418-3:2020
ICS:
59.060.01 Tekstilna vlakna na splošno Textile fibres in general
SIST EN ISO 20418-3:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 20418-3:2020

---------------------- Page: 2 ----------------------
SIST EN ISO 20418-3:2020


EN ISO 20418-3
EUROPEAN STANDARD

NORME EUROPÉENNE

July 2020
EUROPÄISCHE NORM
ICS 59.060.01
English Version

Textiles - Qualitative and quantitative proteomic analysis
of some animal hair fibres - Part 3: Peptide detection using
LC-MS without protein reduction (ISO 20418-3:2020)
Textiles - Analyse protéomique qualitative et Textilien - Qualitative und quantitative
quantitative de certaines fibres animales - Partie 3: Proteomanalyse einiger Tierhaarfasern - Teil 3:
Détection des peptides par LC-MS sans réduction Peptiddetektion mit LC-MS ohne Proteinreduktion (ISO
protéique (ISO 20418-3:2020) 20418-3:2020)
This European Standard was approved by CEN on 7 June 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20418-3:2020 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN ISO 20418-3:2020
EN ISO 20418-3:2020 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------
SIST EN ISO 20418-3:2020
EN ISO 20418-3:2020 (E)
European foreword
This document (EN ISO 20418-3:2020) has been prepared by Technical Committee ISO/TC 38
"Textiles" in collaboration with Technical Committee CEN/TC 248 “Textiles and textile products” the
secretariat of which is held by BSI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2021, and conflicting national standards shall
be withdrawn at the latest by January 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 20418-3:2020 has been approved by CE
...