CEN/TS 15790:2008

Animal feeding stuffs - PCR typing of probiotic strains of Saccharomyces cerevisiae (yeast)

This Technical Specification defines a polymerase chain reaction (PCR) methodology for the identification of S. cerevisiae probiotic yeast strains. Additionally, a method for the extraction of high quality DNA from yeast is suggested.

Futtermittel - PCR-Typisierung der probiotischen Stämme von Saccharomyces cerevisiae (Hefe)

Diese Technische Spezifikation legt eine Polymerase-Kettenreaktion-(PCR)-Methodik für die Identifizierung
von probiotischen Saccharomyces cerevisiae-Hefestämmen fest. Zusätzlich wird ein Verfahren für die
Extraktion hochreiner DNA der Hefe vorgeschlagen.

Aliments des animaux - Typage par réaction de polymérisation en chaîne (PCR) des souches probiotiques de Saccharomyces cerevisiae (levure)

La présente Spécification technique définit une méthode d’amplification en chaîne par polymérase (ACP)
destinée à l’identification des souches de levure probiotiques de Saccharomyces cerevisiae. Elle présente
également une méthode d’extraction d’un ADN de bonne qualité à partir de la levure.

Krma - PCR-tipizacija probiotičnih sevov Saccharomyces cerevisiae (kvasovk)

General Information

• Status: Published
• Publication Date: 09-Dec-2008
• ICS: 07.100.30 - Food microbiology
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: CEN/TC 327 - Animal feeding stuffs - Methods of sampling and analysis
• Drafting Committee: CEN/TC 327/WG 3 - Feed additives and drugs
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 26-May-2011
• Completion Date: 26-May-2011
• Directive: 70/373/EC - Council Directive 70/373/EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feeding-stuffs
• Mandate: M/382 - Mandate for standardisation addressed to CEN in the field of Methods of analysis for animal Feedingstuffs
• Ref Project: SIST-TS CEN/TS 15790:2009 - Animal feeding stuffs - PCR typing of probiotic strains of Saccharomyces cerevisiae (yeast)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 15790:2009
01-april-2009
.UPD3&5WLSL]DFLMDSURELRWLþQLKVHYRY6DFFKDURP\FHVFHUHYLVLDHNYDVRYN
Animal feeding stuffs - PCR typing of probiotic strains of Saccharomyces cerevisiae
(yeast)
Futtermittel - PCR-Typisierung der probiotischen Stämme von Saccharomyces
cerevisiae (Hefe)
Aliments des animaux - Typage ACP des souches probiotiques de Saccharomyces
cerevisiae (levure)
Ta slovenski standard je istoveten z: CEN/TS 15790:2008
ICS:
65.120 Krmila Animal feeding stuffs
SIST-TS CEN/TS 15790:2009 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 15790:2009

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SIST-TS CEN/TS 15790:2009
TECHNICAL SPECIFICATION
CEN/TS 15790
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
December 2008
ICS 07.100.30; 65.120

English Version
Animal feeding stuffs - PCR typing of probiotic strains of
Saccharomyces cerevisiae (yeast)
Aliments des animaux - Typage ACP des souches Futtermittel - PCR-Typisierung der probiotischen Stämme
probiotiques de Saccharomyces cerevisiae (levure) von Saccharomyces cerevisiae (Hefe)
This Technical Specification (CEN/TS) was approved by CEN on 25 August 2008 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 15790:2008: E
worldwide for CEN national Members.

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SIST-TS CEN/TS 15790:2009
CEN/TS 15790:2008 (E)
Contents Page
Foreword .3
Introduction .4
1 Scope .5
2 Principle .5
3 Reagents .5
3.1 PCR .5
3.1.1 Primers .5
3.1.2 dNTP mix .5
3.1.3 Buffer .6
3.1.4 Magnesium Chloride solution .6
3.1.5 DNA Taq polymerase .6
3.2 Gel Electrophoresis.6
3.2.1 Agarose, molecular-grade agarose free from DNase and RNase contamination. .6
3.2.2 Molecular weight marker .6
3.2.3 Tris-Borate-EDTA buffer .
...