EN ISO 17993:2003

Water quality - Determination of 15 polycyclic aromatic hydrocarbons (PAH) in water by HPLC with fluorescence detection after liquid-liquid extraction (ISO 17993:2002)

ISO 17993:2002 specifies a method using high performance liquid chromatography (HPLC) with fluorescence detection for the determination of 15 selected polycyclic aromatic hydrocarbons (PAH) [naphthalene, acenaphthene, phenanthrene, fluoranthene, benzo(a)anthracene, benzo(b)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, fluorene, anthracene, pyrene, chrysene, benzo(k)fluoranthene, indeno(1,2,3-cd)pyrene, benzo(ghi)perylene] in drinking and ground water in mass concentrations greater than 0,005 microgram/litre (for each single compound) and surface waters in mass concentrations above 0,01 microgram/litre.
This method is, with some modification, also suitable for the analysis of waste water. This method may be applicable to other PAH, provided the method is validated for each case.

Wasserbeschaffenheit - Bestimmung von 15 polycyclischen aromatischen Kohlenwasserstoffen (PAK) in Wasser durch HPLC mit Fluoreszenzdetektion nach Flüssig-Flüssig-Extraktion (ISO 17993:2002)

Diese Internationale Norm legt ein Verfahren zur Bestimmung von 15 ausgewählten PAK (siehe Tabelle 1) mit Hilfe der hochauflösenden Dampfraumnanalyse (HPLC) nach flüssig-flüssig-Extraktion und Fluoreszenzs-pektrometrie fest. Das Verfahren wird angewendet auf Trink- und Grundwasser in Massenkonzentrationen oberhalb 0,005 µg/l (Einzelsubstanz) und auf Oberflächenwasser in Massenkonzentrationen oberhalb 0,01 µg/l fest.
Das Verfahren ist nach Anpassung auch geeignet für die Untersuchung von Abwasser. Das Verfahren darf auch auf andere PAK angewendet werden, vorausgesetzt, es wird in jedem Einzelfall validiert.

Qualité de l'eau - Dosage de 15 hydrocarbures aromatiques polycycliques (HAP) dans l'eau par HPLC avec détection par fluorescence après extraction liquide-liquide (ISO 17993:2002)

La présente Norme internationale spécifie une méthode pour le dosage, par chromatographie en phase liquide à haute performance (HPLC) avec détection par fluorescence, de 15 HAP sélectionnés (voir Tableau 1) dans l'eau potable et dans les eaux souterraines à des concentrations en masse supérieures à 0,005 microgrammes par litre (pour chaque composé individuel), et dans les eaux de surface à des concentrations en masse supérieures à 0,01 microgrammes par litre.
Cette méthode est également appropriée, avec certaines modifications, pour l'analyse des eaux usées. Elle peut être appliquée à d'autres HAP, sous réserve de valider la méthode dans chaque cas.

Kakovost vode – Določevanje 15 policikličnih aromatskih ogljikovodikov (PAH) v vodi s tehniko HPLC s fluorescenčno detekcijo po ekstrakciji tekoče-tekoče (ISO 17993:2002)

General Information

• Status: Published
• Publication Date: 25-Nov-2003
• ICS: 13.060.50 - Examination of water for chemical substances
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230/WG 1 - Physical and biochemical methods
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 21-May-2008
• Completion Date: 21-May-2008
• Directive: 01/990 - Proposal for a EP and Council Decision establishing the list of priority substances in the field of water policy COM(2000)47 New version of 01/990: PE-CONS 3645/01 (2001-09-04) : PE and Council Decision on List of Priority Substances for WFD
• Directive: 2000/60/EC - Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy (substitutes Directive Reference 01/991 - Common Position on WFD)
• Directive: 2001/990 - Proposal for a EP and Council Decision establishing the list of priority substances in the field of water policy COM(2000)47 New version of 01/990: PE-CONS 3645/01 (2001-09-04) : PE and Council Decision on List of Priority Substances for WFD
• Directive: 98/83/EC - Quality of water intended for human consumption
• Ref Project: SIST EN ISO 17993:2004 - Water quality - Determination of 15 polycyclic aromatic hydrocarbons (PAH) in water by HPLC with fluorescence detection after liquid-liquid extraction (ISO 17993:2002)

Standards Content (Sample)

SLOVENSKI STANDARD
01-februar-2004
.DNRYRVWYRGH±'RORþHYDQMHSROLFLNOLþQLKDURPDWVNLKRJOMLNRYRGLNRY3$+Y
YRGLVWHKQLNR+3/&VIOXRUHVFHQþQRGHWHNFLMRSRHNVWUDNFLMLWHNRþHWHNRþH,62

Water quality - Determination of 15 polycyclic aromatic hydrocarbons (PAH) in water by
HPLC with fluorescence detection after liquid-liquid extraction (ISO 17993:2002)
Wasserbeschaffenheit - Bestimmung von 15 polycyclischen aromatischen
Kohlenwasserstoffen (PAK) in Wasser durch HPLC mit Fluoreszenzdetektion nach
Flüssig-Flüssig-Extraktion (ISO 17993:2002)
Qualité de l'eau - Dosage de 15 hydrocarbures aromatiques polycycliques (HAP) dans
l'eau par HPLC avec détection par fluorescence apres extraction liquide-liquide (ISO
17993:2002)
Ta slovenski standard je istoveten z: EN ISO 17993:2003
ICS:
13.060.50 3UHLVNDYDYRGHQDNHPLþQH Examination of water for
VQRYL chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 17993
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2003
ICS 13.060.50
English version
Water quality - Determination of 15 polycyclic aromatic
hydrocarbons (PAH) in water by HPLC with fluorescence
detection after liquid-liquid extraction (ISO 17993:2002)
Qualité de l'eau - Dosage de 15 hydrocarbures aromatiques Wasserbeschaffenheit - Bestimmung von 15 polycyclischen
polycycliques (HAP) dans l'eau par HPLC avec détection aromatischen Kohlenwasserstoffen (PAK) in Wasser durch
par fluorescence après extraction liquide-liquide (ISO HPLC mit Fluoreszenzdetektion nach Flüssig-Flüssig-
17993:2002) Extraktion (ISO 17993:2002)
This European Standard was approved by CEN on 3 November 2003.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2003 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17993:2003 E
worldwide for CEN national Members.

Foreword
The text of ISO 17993:2002 has been prepared by Technical Committee ISO/TC 147 "Water
quality” of the International Organization for Standardization (ISO) and has been taken over as
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by May 2004, and conflicting national standards
shall be withdrawn at the latest by May 2004.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium, Czech
Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,
Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and
the United Kingdom.
Endorsement notice
The text of ISO 17993:2002 has been approved by CEN as EN ISO 17993:2003 without any
modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions of
any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications, indicated
by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 5667-2 1991 Water quality - Sampling - Part 2: EN 25667-2 1993
Guidance on sampling techniques
ISO 5667-3 1994 Water quality - Sampling - Part 3: EN ISO 5667-3 1995
Guidance on the preservation and
handling of samples
INTERNATIONAL ISO
STANDARD 17993
First edition
2002-08-15
Water quality — Determination of 15
polycyclic aromatic hydrocarbons (PAH) in
water by HPLC with fluorescence detection
after liquid-liquid extraction
Qualité de l'eau — Dosage de 15 hydrocarbures aromatiques polycycliques
(HAP) dans l'eau par HPLC avec détection par fluorescence après
extraction liquide-liquide
Reference number
ISO 17993:2002(E)
©
ISO 2002
ISO 17993:2002(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not
be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this
file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this
area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters
were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event
that a problem relating to it is found, please inform the Central Secretariat at the address given below.

©  ISO 2002
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic
or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body
in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.ch
Printed in Switzerland
ii © ISO 2002 – All rights reserved

ISO 17993:2002(E)
Contents Page
Foreword . iv
Introduction. v
1 Scope. 1
2 Normative references. 1
3 Principle . 1
4 Interferences. 3
5 Reagents . 4
6 Apparatus. 5
7 Sampling . 6
8 Procedure. 6
9 Calculation . 11
10 Precision . 11
11 Test report. 13
Annex A (informative) Examples of chromatographic conditions and columns . 14
Annex B (informative) Examples for the construction of special apparatus. 18

ISO 17993:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
The main task of technical committees is to prepare International Standards. Draft International Standards adopted
by the technical committees are circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 17993 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2, Physical,
chemical and biochemical methods.
Annexes A and B of this International Standard are for information only.
iv © ISO 2002 – All rights reserved

ISO 17993:2002(E)
Introduction
Polycyclic aromatic hydrocarbons (PAH) occur in nearly all types of waters. These compounds are adsorbed on
solids (sediments, suspended matter) as well as dissolved in the liquid phase.
Some PAH are known or suspected to cause cancer. The Council Directive 98/83/EC on the quality of water
intended for human consumption set the maximum acceptable level for benzo(a)pyrene at 0,010 µg/l, and for the
sum of four specified PAH [benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(ghi)perylene, indeno(1,2,3-cd)-
pyrene] at 0,100 µg/l.
INTERNATIONAL STANDARD ISO 17993:2002(E)

Water quality — Determination of 15 polycyclic aromatic
hydrocarbons (PAH) in water by HPLC with fluorescence detection
after liquid-liquid extraction
WARNING — Some compounds being measured are presumed to be carcinogenic. Acetonitrile and hexane
are toxic.
Persons using this International Standard should be familiar with normal laboratory practice. This
International Standard does not purport to address all of the safety problems, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure
compliance with any national regulatory conditions.
1 Scope
This International Standard specifies a method using high performance liquid chromatography (HPLC) with
fluorescence detection after liquid-liquid extraction for the determination of 15 selected PAH (see Table 1) in
drinking and ground water in mass concentrations greater than 0,005 µg/l (for each single compound) and surface
waters in mass concentrations above 0,01 µg/l.
This method is, with some modification, also suitable for the analysis of wastewater. This method may be
applicable to other PAH, provided the method is validated for each case.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent editions of the normative documents indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 5667-2, Water quality — Sampling — Part 2: Guidance on sampling techniques
ISO 5667-3, Water quality — Sampling — Part 3: Guidance on the preservation and handling of samples
ISO 8466-1, Water quality — Calibration and evaluation of analytical methods and estimation of performance
characteristics — Part 1: Statistical evaluation of the linear calibration function
3 Principle
The PAH present in the aqueous sample are extracted from the water sample with hexane. The extract is
concentrated by evaporation and the residue taken up in a solvent appropriate for HPLC analysis.
If necessary, extracts of surface water or more contaminated water samples are cleaned by chromatography over
silica prior to analysis.
PAH are separated by HPLC on a suitable stationary phase using gradient elution. Identi
...