ISO 20128:2006

Milk products — Enumeration of presumptive Lactobacillus acidophilus on a selective medium — Colony-count technique at 37 degrees C

ISO 20128|IDF 192:2006 specifies a method for the enumeration of presumptive Lactobacillus acidophilus in milk products on a selective medium by using a colony-count technique at 37 °C. The method is applicable to fermented and non-fermented milks, milk powders and infant formulae where presumptive L. acidophilus is present and in combination with other lactic acid bacteria and bifidobacteria. The method is not applicable when the number of presumptive L. acidophilus is less than 104 CFU/g and the numbers of Lactobacillus rhamnosus, Lactobacillus reuteri and Lactobacillus paracasei subsp. paracasei are greater than 106 CFU/g.

Produits laitiers — Dénombrement de Lactobacillus acidophilus présomptifs sur un milieu sélectif — Technique de comptage des colonies à 37 degrés C

General Information

• Status: Published
• Publication Date: 11-May-2006
• ICS: 07.100.30 - Food microbiology
• ICS: 67.100.10 - Milk and processed milk products
• Technical Committee: ISO/TC 34/SC 5 - Milk and milk products
• Drafting Committee: ISO/TC 34/SC 5 - Milk and milk products
• Current Stage: 9093 - International Standard confirmed
• Completion Date: 28-Sep-2023

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 20128
IDF
192
First edition
2006-05-15

Milk products — Enumeration of
presumptive Lactobacillus acidophilus on
a selective medium — Colony-count
technique at 37 °C
Produits laitiers — Dénombrement de Lactobacillus acidophilus
présomptifs sur un milieu sélectif — Technique de comptage des
colonies à 37 °C




Reference numbers
ISO 20128:2006(E)
IDF 192:2006(E)
©
ISO and IDF 2006

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ISO 20128:2006(E)
IDF 192:2006(E)
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ii © ISO and IDF 2006 – All rights reserved

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ISO 20128:2006(E)
IDF 192:2006(E)
Contents Page
Foreword. iv
Foreword. v
Introduction . vi
1 Scope .1
2 Normative references .1
3 Terms and definitions .1
4 Principle.2
5 Diluents, culture media and reagents.2
5.1 Basic materials.2
5.2 Diluent.2
5.3 Culture media.2
6 Apparatus and glassware .4
7 Sampling.5
8 Procedure .5
8.1 Sample preparation and decimal dilutions .5
8.2 Inoculation and incubation .6
8.3 Enumeration of colonies.6
9 Calculation and expression of results.
...