SIST EN ISO 22517:2021

Leather - Chemical tests - Determination of pesticide residues content (ISO 22517:2019)

This document specifies a quantitative test method to determine 24 kinds of pesticide residues in leather by gas chromatography-mass spectrometry (GC-MS).
This document is applicable to all types of leather that could release pesticides.

Leder - Chemische Prüfungen - Bestimmung des Pestizidrückstandsgehalts (ISO 22517:2019)

Dieses Dokument legt ein quantitatives Prüfverfahren zur Bestimmung von 24 Arten von Pestizidrückständen in Leder mittels Gaschromatographie-Massenspektrometrie (GC MS) fest.
Dieses Dokument ist anzuwenden für alle Lederarten, die Pestizide freisetzen könnten.

Cuir - Essais chimiques - Détermination de la teneur en résidus de pesticides (ISO 22517:2019)

Le présent document spécifie une méthode d'essai quantitative visant à déterminer 24 types de résidus de pesticides dans le cuir, par chromatographie en phase gazeuse couplée à la spectrométrie de masse (CG-SM).
Il est applicable à tous les types de cuir susceptibles de libérer des pesticides.

Usnje - Kemični preskusi - Določevanje ostankov pesticidov (ISO 22517:2019)

General Information

• Status: Published
• Public Enquiry End Date: 02-May-2021
• Publication Date: 29-Aug-2021
• ICS: 59.140.30 - Leather and furs
• ICS: 65.100.99 - Other pesticides and agrochemicals
• Technical Committee: IUSN - Leather
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 25-Aug-2021
• Due Date: 30-Oct-2021
• Completion Date: 30-Aug-2021
• Ref Project: EN ISO 22517:2021 - Leather - Chemical tests - Determination of pesticide residues content (ISO 22517:2019)

Overview

EN ISO 22517:2021 (identical to ISO 22517:2019) - titled Leather - Chemical tests - Determination of pesticide residues content - specifies a quantitative GC‑MS method for determining pesticide residues in leather. The standard covers the analysis of 24 pesticide compounds (listed in Annex A) and is applicable to all types of leather that could release pesticides. It was prepared by IULTCS in collaboration with CEN and adopted as a European standard in 2021.

Key topics and requirements

• Scope and principle: A quantitative method using gas chromatography–mass spectrometry (GC‑MS) with external standard calibration.
• Sample extraction: Ultrasonic extraction using n‑hexane : ethyl acetate (1 + 1, volume).
• Reagents and standards: Requirements for chromatographic‑grade solvents; preparation of pesticide standard solutions (example concentrations given: 100 µg/ml working, 1 µg/ml stock, calibration range 10–1 000 ng/ml).
• Apparatus: GC with mass‑selective detector (electron impact ionization), analytical balances, vortex, ultrasonic bath (40 kHz, 200 W), rotary evaporator, etc.
• Procedure elements: Extraction, purification, preparation of blanks, calibration solutions, GC‑MS analysis, and system checks.
• Data evaluation: Calculation of concentrations, recovery, precision and reliability assessment.
• Safety: Emphasis on high toxicity of pesticides and POPs; follow material safety data sheets and good laboratory practice.
• Supporting annexes: Detailed lists and GC‑MS parameters are provided - e.g., chromatographic conditions, retention times, ions for quantitation/confirmation, recovery ranges and precision data.

Practical applications

• Routine and confirmatory testing of leather products (raw, semi‑finished, finished) for pesticide contamination.
• Quality control in leather manufacturing to ensure product safety and regulatory compliance.
• Analytical service and environmental laboratories performing GC‑MS pesticide residue analysis.
• Compliance testing for import/export, consumer safety, occupational exposure and environmental monitoring where leather may release pesticide residues.

Who should use this standard

• Leather testing laboratories and QA/QC teams in tanneries.
• Third‑party testing and certification bodies.
• Regulatory agencies overseeing chemical safety in textiles and leather goods.
• R&D groups developing extraction and analytical workflows for multi‑residue GC‑MS methods.

Related standards

• ISO 2418 - Leather: sampling location for chemical, physical and fastness tests.
• ISO 4044 - Leather: preparation of chemical test samples.

Keywords: EN ISO 22517:2021, ISO 22517:2019, pesticide residues, leather testing, GC‑MS, gas chromatography‑mass spectrometry, analytical method, leather chemical tests.

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2021
Usnje - Kemični preskusi - Določevanje ostankov pesticidov (ISO 22517:2019)
Leather - Chemical tests - Determination of pesticide residues content (ISO 22517:2019)
Leder - Chemische Prüfungen - Bestimmung des Pestizidrückstandsgehalts (ISO
22517:2019)
Cuir - Essais chimiques - Détermination de la teneur en résidus de pesticides (ISO
22517:2019)
Ta slovenski standard je istoveten z: EN ISO 22517:2021
ICS:
59.140.30 Usnje in krzno Leather and furs
65.100.99 Drugi pesticidi in Other pesticides and
agrokemikalije agrochemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 22517
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2021
EUROPÄISCHE NORM
ICS 59.140.30
English Version
Leather - Chemical tests - Determination of pesticide
residues content (ISO 22517:2019)
Cuir - Essais chimiques - Détermination de la teneur en Leder - Chemische Prüfungen - Bestimmung des
résidus de pesticides (ISO 22517:2019) Pestizidrückstandsgehalts (ISO 22517:2019)
This European Standard was approved by CEN on 9 August 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22517:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 22517:2019 has been prepared by Technical Committee ISO/IULTCS "International
Union of Leather Technologists and Chemists Societies” of the International Organization for
Standardization (ISO) and has been taken over as EN ISO 22517:2021 by Technical Committee CEN/TC
289 “Leather” the secretariat of which is held by UNI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2022, and conflicting national standards
shall be withdrawn at the latest by February 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 22517:2019 has been approved by CEN as EN ISO 22517:2021 without any modification.

INTERNATIONAL ISO
STANDARD 22517
IULTCS/IUC 38
First edition
2019-05
Leather — Chemical tests —
Determination of pesticide residues
content
Cuir — Essais chimiques — Détermination de la teneur en résidus de
pesticides
Reference numbers
ISO 22517:2019(E)
IULTCS/IUC 38:2019(E)
©
ISO 2019
ISO 22517:2019(E)
IULTCS/IUC 38:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 22517:2019(E)
IULTCS/IUC 38:2019(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Safety precautions . 1
6 Reagents . 2
7 Apparatus . 2
8 Procedure. 3
8.1 Sample . 3
8.2 Extraction . 3
8.3 Purification . 3
8.4 Calibration solutions . 4
8.4.1 Preparation of calibration solution mixture . 4
8.4.2 Preparation of calibration mixture for recovery rate . 4
8.5 Preparation of blank sample . 4
8.6 Analysis by GC-MS . 4
8.7 Check of the analytical system . 4
9 E valuation of results . 5
9.1 Calculation of results . 5
9.2 Reliability of the method . 5
10 Test report . 5
Annex A (normative) 24 kinds of pesticide compounds . 6
Annex B (informative) GC-MS chromatographic conditions and parameters.7
Annex C (informative) Retention time, quantitative and qualitative ions and ion abundance
ratios . 8
Annex D (informative) Start time and dwell time of each group of detecting ions.9
Annex E (informative) Chromatogram and selected ion of the 24 pesticides standard
obtained by GC-MS .10
Annex F (informative) Recovery range of pesticides in leather (n = 6) .11
Annex G (informative) Precision .13
Bibliography .15
ISO 22517:2019(E)
IULTCS/IUC 38:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
This document was prepared by the Chemical Tests Commission of the International Union of Leather
Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, the secretariat of
which is held by UNI, in accordance with the Agreement on technical cooperation between ISO and CEN
(Vienna Agreement).
IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to
further the advancement of leather science and technology. IULTCS has three Commissions, which
are responsible for establishing international methods for the sampling and testing of leather. ISO
recognizes IULTCS as an international standardizing body for the preparation of test methods for
leather.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
iv © ISO 2019 – All rights reserved

ISO 22517:2019(E)
INTERNATIONAL STANDARD
IULTCS/IUC 38:2019(E)
Leather — Chemical tests — Determination of pesticide
residues content
1 Scope
This document specifies a quantitative test method to determine 24 kinds of pesticide residues in
leather by gas chromatography-mass spectrometry (GC-MS).
This document is applicable to all types of leather that could release pesticides.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 2418, Leather — Chemical, physical and mechanical and fastness tests — Sampling location
ISO 4044, Leather — Chemical tests — Preparation of chemical test samples
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https: //www .iso .org/obp
— IEC Electropedia: available at http: //www .electropedia .org/
4 Principle
The leather sample is ultrasonically extracted with a mixed solution of n-hexane and ethyl acetate
(1 + 1, volume). The extraction is detected and confirmed by GC-MS, and quantified using an external
standard method.
5 Safety precautions
5.1 The compounds of pesticides are classified as high toxicity substances. Some are persistent organic
pollutants (POPs) and suspected to be human carcinogens.
Any handling and disposal of these substances shall be in strict accordance with the applicable health
and safety requirements.
5.2 It is the user's responsibility to use safe and proper techniques when handling materials in
this test method. Consult manufacturers for specific details, such as safety data sheets and other
recommendations.
5.3 Good laboratory practice should be followed. Wear safety glasses in all laboratory areas, as well as
a dust respirator and single-use gloves while handling those compounds and leather samples.
ISO 22517:2019(E)
IULTCS/IUC 38:2019(E)
6 Reagents
Unless otherwise specified, analytical grade chemicals shall be used.
6.1 n-Hexane, chromatographic pure.
6.2 Ethyl acetate, chromatographic pure.
6.3 Acetonitrile, chromatographic pure.
6.4 Toluene, chromatographic pure.
6.5 n-Hexane and ethyl acetate (1 + 1, volume) mixed solution.
6.6 Acetonitrile and toluene (3 + 1, volume) mixed solution.
6.7 24 kinds of pesticide standards, as listed in Annex A, purity of 97 % or more. See Annex A for
individual details of the 24 pesticide compounds.
6.8 Standard solutions of pesticides, accurately weigh the amount of each pesticide standard, and
dissolve in a small amount of n-hexane to prepare a concentration 100 μg/ml of standard solution. A
commercially available certified solution is an alternative. The shelf life of commercially available certified
solution is given in the certificate. The shelf life of the solution prepared from powder is one month.
6.9 Standard stock solutions of pesticides, accurately pipette a certain volume of each standard
pesticide solution (6.8) into a separate volumetric flask. Dilute with n-hexane to prepare the intermediate
standard stock solution with a concentration of 1 μg/ml. The shelf life of the stock solution is one month.
6.10 Standard calibration solutions of pesticides, accurately pipette a certain volume of standard
stock solution (6.9) into a volumetric flask and dilute with n-hexane to prepare standard calibration
solutions with concentrations in the range of 10 ng/ml to 1 000 ng/ml. Prepare them before using.
7 Apparatus
Usual laboratory equipment and, in particular, the following.
7.1 Gas chromatograph (GC), with mass-selective detector (MSD) and electron impact source (EIS).
7.2 Analytical balance, weighing to an accuracy of 0,1 mg.
7.3 Laboratory balance, weighing to an accuracy of 0,01 g.
7.4 Vortex mixer.
7.5 Ultrasonic water-bath, ultrasonic frequency 40 KHz, ultrasonic power 200 W, controllable
heating.
7.6 Vacuum rotary evaporator, with vacuum control and water bath. Other types of evaporation
apparatus may be used, e.g. a water bath with a controlled flow of nitrogen over the liquid.
7.7 Solid phase extraction (SPE) system, with vacuum device and graphitized carbon black (Carb),
solid phase extraction column, 500 mg, 6 ml.
2 © ISO 2019 – All rights reserved

ISO 22517:2019(E)
IULTCS/IUC 38:2019(E)
7.8 Small volume pipetting device, 1 ml to 5 ml, and 100 μl to 1 000 μl.
7.9 Centrifuge, with a revolution speed of more than 5 000 r/min.
7.10 Stoppered polypr
...