SIST EN 16294:2013

Petroleum products and fat and oil derivatives - Determination of phosphorus content in fatty acid methyl esters (FAME) - Optical emission spectral analysis with inductively coupled plasma (ICP OES)

This European Standard specifies an inductively coupled plasma optical emission spectrometry (ICP OES) method for the determination of phosphorus content of Fatty Acid Methyl Esters (FAME) in the range of 2,5 mg/kg to 8,0 mg/kg.
WARNING - The use of this standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Mineralölprodukte und Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Bestimmung des Gehalts an Phosphor in Fettsäuremethylestern (FAME) - Direkte Bestimmung durch optische Emissionsspektralanalyse mit induktiv gekoppeltem Plasma (ICP OES)

Diese Europäische Norm legt ein Verfahren zur Bestimmung des Phosphorgehalts in Fettsäuremethylestern (FAME) im Bereich von 2,5 mg/kg bis 8,0 mg/kg mit Hilfe der optischen Emissionsspektrometrie mit induktiv gekoppeltem Plasma (ICP OES) fest. WARNUNG — Die Anwendung dieses Dokument kann den Einsatz gefährlicher Stoffe, Arbeitsgänge und Geräte mit sich bringen. Dieses Dokument gibt nicht vor, alle mit seiner Anwendung verbundenen Sicherheitsprobleme anzusprechen. Der Anwender dieses Dokuments ist dafür verantwortlich, vorher angemessene Maßnahmen in Hinblick auf Sicherheit und Gesundheit zu ergreifen und die Anwendbarkeit einschränkender Vorschriften zu ermitteln.

Produits pétroliers et produits dérivés des corps gras - Détermination de la teneur en phosphore des esters méthyliques d'acides gras (EMAG) - Méthode par spectrométrie d'émission atomique à couplage inductif par plasma (ICP OES)

La présente Norme Européenne spécifie une méthode pour la détermination de la teneur en phosphore des esters méthyliques d’acides gras (EMAG) par spectrométrie d’émission atomique par plasma à couplage inductif (ICP OES). Cette méthode est applicable à des teneurs en phosphore comprises entre 2,5 mg/kg et 8,0 mg/kg.
AVERTISSEMENT - L'utilisation de la présente Norme européenne peut impliquer l'intervention de produits, d'opérations et d'équipements à caractère dangereux. La présente Norme européenne n'est pas censée aborder tous les problèmes de sécurité concernés par son usage. Il est de la responsabilité de l'utilisateur de consulter et d'établir des règles de sécurité et d'hygiène appropriées et de déterminer l'applicabilité des restrictions réglementaires avant utilisation.

Naftni proizvodi in derivati maščob ter olj - Določevanje fosforja v metilnih estrih maščobnih kislin (FAME) - Optična emisijska spektrometrija z induktivno sklopljeno plazmo (ICP OES)

Ta evropski standard določa metodo optične emisijske spektrometrije z induktivno sklopljeno plazmo (ICP OES) za določevanje fosforja v metilnih estrih maščobnih kislin (FAME) v razponu od 2,5 mg/kg do 8,0 mg/kg.

General Information

• Status: Published
• Public Enquiry End Date: 16-Aug-2011
• Publication Date: 08-Jan-2013
• ICS: 75.160.20 - Liquid fuels
• Technical Committee: NAD - Petroleum products, lubricants and related products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 14-Dec-2012
• Due Date: 18-Feb-2013
• Completion Date: 09-Jan-2013
• Ref Project: EN 16294:2012 - Petroleum products and fat and oil derivatives - Determination of phosphorus content in fatty acid methyl esters (FAME) - Optical emission spectral analysis with inductively coupled plasma (ICP OES)

Overview

EN 16294:2012 (CEN) defines an analytical method for the determination of phosphorus content in Fatty Acid Methyl Esters (FAME) using inductively coupled plasma optical emission spectrometry (ICP OES). The standard covers direct optical emission spectral analysis of FAME samples and is intended for phosphorus concentrations in the range 2.5 mg/kg to 8.0 mg/kg. A general safety warning highlights that hazardous materials and equipment may be involved and that users must adopt appropriate safety practices.

Key topics and technical requirements

• Scope and target analyte: Phosphorus in FAME (2.5–8.0 mg/kg).
• Analytical technique: ICP OES (optical emission spectrometry with inductively coupled plasma). Radial ICP-OES is recommended to minimize carbon-related matrix effects.
• Sample preparation: Weigh FAME, dilute with kerosene (boiling range 175–250 °C) and use oil-based stock and intermediate standards. Use plastic bottles (HDPE, FEP, PFA) to avoid contamination.
• Internal standard: Use element standards such as cobalt, scandium or yttrium to correct viscosity/matrix effects.
• Calibration: Two procedures described (A and B) that use intensity ratios or corrected intensities; calibration uses multiple standards and linear regression. Recommended P emission lines include 177.499 nm, 178.287 nm, 213.618 nm, 214.914 nm.
• Quality control: Regular calibration checks with QC samples; if QC differs by more than 20% from reference, recalibrate.
• Equipment and reagents: High-purity argon (≥ 99.996%), appropriate nebuliser and pump tubing for organic solvents, analytical balance (0.1 mg), volumetric flasks and solvent-cleaning protocols to avoid phosphorus contamination.
• Performance data: Contains precision statements (repeatability and reproducibility) and reporting requirements.

Applications and users

• Biodiesel producers and quality control labs assessing phosphorus in FAME to ensure fuel quality and protect engine and exhaust after‑treatment systems.
• Fuel testing and certification laboratories performing routine compositional analysis and regulatory compliance testing.
• Research laboratories studying feedstock impacts and refining processes that affect phosphorus levels.
• Refineries and blending facilities monitoring additives and contaminants during production and blending.

Practical benefits: accurate low-level phosphorus measurement, matrix correction via internal standards, and standardized calibration/QA procedures for consistent results across laboratories.

Related standards

• EN ISO 1042 - Laboratory glassware: one-mark volumetric flasks
• EN ISO 3170 - Petroleum liquids - Manual sampling
• EN ISO 3171 - Petroleum liquids - Automatic pipeline sampling

Keywords: EN 16294:2012, FAME phosphorus analysis, ICP OES, inductively coupled plasma, phosphorus in biodiesel, calibration, internal standard, fuel testing, CEN.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.NWLYQRMineralölprodukte und Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Bestimmung des Gehalts an Phosphor in Fettsäuremethylestern (FAME) - Direkte Bestimmung durch optische Emissionsspektralanalyse mit induktiv gekoppeltem Plasma (ICP OES)Produits pétroliers et produits dérivés des corps gras - Détermination de la teneur en phosphore des esters méthyliques d'acides gras (EMAG) - Méthode par spectrométrie d'émission atomique à couplage inductif par plasma (ICP OES)Petroleum products and fat and oil derivatives - Determination of phosphorus content in fatty acid methyl esters (FAME) - Optical emission spectral analysis with inductively coupled plasma (ICP OES)75.160.20Liquid fuelsICS:Ta slovenski standard je istoveten z:EN 16294:2012SIST EN 16294:2013en,de01-februar-2013SIST EN 16294:2013SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16294
November 2012 ICS 75.160.20 English Version
Petroleum products and fat and oil derivatives - Determination of phosphorus content in fatty acid methyl esters (FAME) - Optical emission spectral analysis with inductively coupled plasma (ICP OES)
Produits pétroliers et produits dérivés des corps gras - Détermination de la teneur en phosphore des esters méthyliques d'acides gras (EMAG) - Méthode par spectrométrie d'émission atomique par plasma
à couplage inductif (ICP OES)
Mineralölprodukte und Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Bestimmung des Gehalts an Phosphor in Fettsäuremethylestern (FAME) - Direkte Bestimmung durch optische Emissionsspektralanalyse mit induktiv gekoppeltem Plasma (ICP OES) This European Standard was approved by CEN on 13 October 2012.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16294:2012: ESIST EN 16294:2013

4.4 Phosphorus intermediate solution (25 mg/kg).
Weigh 0,50 g ± 0,01 g of phosphorus standard solution (4.3) into a 50 ml bottle (5.2). Add kerosene (4.2) to 20,00 g ± 0,01 g. Each mass shall be weighed to the nearest 0,1 mg. SIST EN 16294:2013
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