EN ISO 23611-4:2011

Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and identification of soil-inhabiting nematodes (ISO 23611-4:2007)

ISO 23611-4:2007 specifies a method for sampling and handling free-living nematodes from terrestrial field soils as a prerequisite for using them as bio-indicators (e.g. to assess the quality of a soil as a habitat for organisms).
ISO 23611-4:2007 applies to all terrestrial biotopes in which nematodes occur. It is not applicable to aquatic nematodes because these nematodes do not pass through the filter.

Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 4: Probenahme, Extraktion und Bestimmung von Boden bewohnenden Nematoden (ISO 23611-4:2007)

Dieser Teil von ISO 23611 legt ein Verfahren zur Probenahme und Handhabung frei lebender Nematoden aus terrestrischen Freilandböden fest, das als eine Voraussetzung für die Verwendung dieser Tiere als Bioindikatoren (z. B. zur Beurteilung der Beschaffenheit eines Bodens als Lebensraum für Organismen) dient. Dieser Teil von ISO 23611 gilt für alle terrestrischen Biotope, in denen Nematoden vorkommen. Die allgemeine Planung der Probenahme für Freilanduntersuchungen ist in ISO 10381-1 festgelegt. Dieser Teil von ISO 23611 gilt nicht für aquatische Nematoden, da diese nicht durch das Filter hindurchgehen. Die Verfahren für einige andere Bodenorganismengruppen wie z. B. Regenwürmer, Enchytraeen oder Collembolen werden in anderen Teilen von ISO 23611 behandelt. Bei den Nematoden, die mit dem vorgeschlagenen Verfahren charakterisiert werden, handelt es sich um alle im Boden vorkommenden, frei lebenden Formen von Nematoden. Sie umfassen Nematoden, die sich nicht von Pflanzen ernähren, sowie auch ektoparasitäre pflanzenfressende Nematoden und frei lebende Stadien endoparasitärer Nematoden. Die mengenmäßige Bestimmung obligater pflanzenfressender Nematoden in Wurzeln erfordert besondere Verfahren. ANMERKUNG Grundlegende Informationen zur Ökologie von Nematoden und ihrer Verwendung als Bioindikatoren sind im Literaturverzeichnis zu finden. Dieser Teil von ISO 23611 behandelt nicht die Charakterisierung des Standortes nach bodenkundlichen Gesichtspunkten, die für die Probenahme von Wirbellosen im Boden nachdrücklich empfohlen wird, da eine enge Beziehung zwischen dem Vorkommen bestimmter Arten und abiotischen Faktoren besteht. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 und ISO 11465 sind zur Bestimmung des pH-Wertes, der Korngrößenverteilung, des C/N-Verhältnisses, des Gehaltes an organischem Kohlenstoff und des Wasserrückhaltevermögens geeigneter.

Qualité du sol - Prélèvement des invertébrés du sol - Partie 4: Prélèvement, extraction et identification des nématodes du sol (ISO 23611-4:2007)

L'ISO 23611-4:2007 spécifie une méthode pour échantillonner et manipuler les nématodes du sol en tant que condition préalable pour les utiliser comme bio-indicateurs (par exemple pour évaluer la qualité d'un sol en tant qu'habitat pour des organismes).
L'ISO 23611-4:2007 s'applique à tous les biotopes terrestres dans lesquels des nématodes sont présents. Elle n'est pas applicable pour les nématodes aquatiques parce que ceux-ci ne traversent pas le filtre.

Kakovost tal - Vzorčenje nevretenčarjev v tleh - 4. del: Vzorčenje, ekstrakcija in identifikacija nematod iz tal  (ISO 23611-4:2007)

ISO 23611-4:2007 določa metodo za vzorčenje in delo s prostoživečimi nematodi iz poljskih tal kot nujen pogoj za uporabo teh živali kot bioindikatorjev (npr. za ocenjevanje kakovosti tal kot habitata za organizme).
ISO 23611-4:2007 velja za vse zemeljske biotope, v katerih so nematodi. Ne velja za vodne nematode, ker ti ne prehajajo skozi filter.

General Information

• Status: Withdrawn
• Publication Date: 12-Jul-2011
• Withdrawal Date: 30-Aug-2022
• ICS: 13.080.05 - Examination of soils in general
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444/WG 4 - Biological characterization
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Completion Date: 31-Aug-2022
• Ref Project: SIST EN ISO 23611-4:2012 - Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and identification of soil-inhabiting nematodes (ISO 23611-4:2007)

Relations

• Replaced By: EN ISO 23611-4:2022 - Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and identification of soil-inhabiting nematodes (ISO 23611-4:2022)
• Effective Date: 13-Feb-2019

Standards Content (Sample)

SLOVENSKI STANDARD
01-januar-2012
.DNRYRVWWDO9]RUþHQMHQHYUHWHQþDUMHYYWOHKGHO9]RUþHQMHHNVWUDNFLMDLQ
LGHQWLILNDFLMDQHPDWRGL]WDO,62
Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and
identification of soil-inhabiting nematodes (ISO 23611-4:2007)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 4: Probenahme,
Extraktion und Bestimmung von Boden bewohnenden Nematoden (ISO 23611-4:2007)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 4: Prélèvement, extraction et
identification des nématodes du sol (ISO 23611-4:2007)
Ta slovenski standard je istoveten z: EN ISO 23611-4:2011
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 23611-4
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2011
ICS 13.080.30; 13.080.05
English Version
Soil quality - Sampling of soil invertebrates - Part 4: Sampling,
extraction and identification of soil-inhabiting nematodes (ISO
23611-4:2007)
Qualité du sol - Prélèvement des invertébrés du sol - Partie Bodenbeschaffenheit - Probenahme von Wirbellosen im
4: Prélèvement, extraction et identification des nématodes Boden - Teil 4: Probenahme, Extraktion und Bestimmung
du sol (ISO 23611-4:2007) von Boden bewohnenden Nematoden (ISO 23611-4:2007)
This European Standard was approved by CEN on 17 June 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-4:2011: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
The text of ISO 23611-4:2007 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 23611-4:2011 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at
the latest by January 2012.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 23611-4:2007 has been approved by CEN as a EN ISO 23611-4:2011 without any
modification.
INTERNATIONAL ISO
STANDARD 23611-4
First edition
2007-11-15
Soil quality — Sampling of soil
invertebrates —
Part 4:
Sampling, extraction and identification of
soil-inhabiting nematodes
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 4: Prélèvement, extraction et identification des nématodes du sol

Reference number
ISO 23611-4:2007(E)
©
ISO 2007
ISO 23611-4:2007(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.

©  ISO 2007
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Published in Switzerland
ii © ISO 2007 – All rights reserved

ISO 23611-4:2007(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Terms and definitions. 1
3 Principle. 2
4 Reagents. 3
5 Apparatus . 3
5.1 Sampling. 4
5.2 Extraction . 4
5.3 Counting . 4
5.4 Fixation and preparation of mass slides. 5
5.5 Identification. 5
6 Procedure . 5
6.1 General. 5
6.2 Sampling. 5
6.3 Extraction . 6
6.4 Counting . 7
6.5 Fixation and preparation of mass slides. 7
6.6 Identification. 8
7 Data assessment. 8
8 Study report. 9
Annex A (informative) Figures of equipment and methods for nematological research. 10
Annex B (informative) Information about the availability of the Oostenbrink elutriator. 13
Annex C (informative) Information about the Baermann funnel/tray extraction method . 15
Bibliography . 17

ISO 23611-4:2007(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 23611-4 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
methods.
ISO 23611 consists of the following parts, under the general title Soil quality — Sampling of soil invertebrates:
⎯ Part 1: Hand-sorting and formalin extraction of earthworms
⎯ Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina)
⎯ Part 3: Sampling and soil extraction of enchytraeids
⎯ Part 4: Sampling, extraction and identification of soil-inhabiting nematodes
iv © ISO 2007 – All rights reserved

ISO 23611-4:2007(E)
Introduction
This part of ISO 23611 has been drawn up since there is a growing need for the standardization of terrestrial
zoological field methods. Such methods, mainly covering the sampling, extraction and handling of soil
invertebrates, are necessary for the following purposes:
[15],[17],[28]
⎯ biological classification of soils including soil quality assessment ;
[9],[10],[13],[24]
⎯ terrestrial bio-indication and long-term monitoring ;
⎯ evaluation of the effects of chemicals on soil animals (ISO 11268-3).
Data for these purposes are gained by standardized methods since they can form the basis for far-reaching
decisions (e.g. whether a given site should be remediated or not). In fact, the lack of such standardized
methods is one of the most important reasons why bio-classification and bio-assessment in terrestrial (i.e. soil)
habitats has so far been relatively rarely used in comparison to aquatic sites.
Nematodes are an important and major part of the soil fauna. Some authors estimate that this group is
probably the most dominant one of the multicellular organisms (Metazoa) on earth. Nematodes occur from the
Antarctic to the tropics and from deep sea sediments to mountain regions. They are active in every place with
sufficient water and organic material. The species diversity and functional variety are impressive. Nematodes
are commonly known as parasites of animals and plants, but the major part of the nematode fauna
participates in decomposition processes by feeding on bacteria and fungi.
Nematodes occur in high numbers [(5 000 to 100 000)/kg fresh soil] and with a high (20 to 100) species
diversity in almost every soil sample. Moreover, there is a broad ecological spectrum of feeding types and
food web relations among the nematodes such as bacterivores, fungivores, herbivores, predators and
[27],[28]
omnivores . These factors make the group highly suitable as indicators for ecological soil
...