IWA 32:2019
(Main)Screening of genetically modified organisms (GMOs) in cotton and textiles
Screening of genetically modified organisms (GMOs) in cotton and textiles
This document provides requirements and recommendations to laboratories that perform genetically modified organism (GMO) analyses in cottonseed, leaf, cotton fibre and cotton fibre-derived materials. The following are within the scope of this document: a) identifying the materials to be assessed, based on the probability of obtaining good quality, fit for purpose DNA from the materials in subsequent steps in the cotton cloth production process; b) specifying a method for efficient DNA isolation from cotton and cotton-derived materials described under point a); c) specifying the cotton-specific method(s) to be used as control for amplifiable DNA; d) specifying the screening procedure that provides optimal chances to detect GMOs as a result of the performance of the lowest number of genetically modified (GM) element screening assays. NOTE 1 The protocol allows for the screening of all currently known GM cotton events and is set up in a way that optimizes the probability of also detecting unknown GM cotton events that possibly contain similar DNA sequences. Further information is given in CEN/TS 16707. Sampling is outside of the scope of this document. NOTE 2 A recommended sampling method is given in ISO 6497. General guidance for the sampling of bulk materials or for cotton-based products is available in standards such as ASTM D1441‑12 and CEN/TS 15568.
Criblage pour la détection des organismes génétiquement modifiés (OGM) dans le coton et les textiles
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INTERNATIONAL IWA
WORKSHOP 32
AGREEMENT
First edition
2019-04
Screening of genetically modified
organisms (GMOs) in cotton and
textiles
Criblage pour la détection des organismes génétiquement modifiés
(OGM) dans le coton et les textiles
Reference number
©
ISO 2019
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Sample preparation . 2
6 DNA isolation . 3
6.1 General . 3
6.2 Principle . 3
6.3 Chemicals, reagents and equipment. 4
6.3.1 Reagents . 4
6.3.2 Apparatus and equipment . 4
6.4 Procedure . 5
6.4.1 General. 5
6.4.2 Protocol . 5
6.5 Results . 6
6.5.1 Analysis . 6
7 DNA quality control . 6
7.1 General . 6
7.2 Principle . 6
7.3 Chemicals, reagents and equipment, including reference materials. 6
7.3.1 Reagents . 6
7.3.2 Apparatus and equipment . 7
7.4 Procedure . 8
7.4.1 General. 8
7.4.2 Safety precautions . 8
7.4.3 Pre-treatment . 8
7.4.4 Amount of sample . 8
7.4.5 Procedure . 8
7.5 Results . 8
7.5.1 Calculations . 8
7.5.2 Interpretation and expression of results . 8
7.5.3 Results . 8
8 GM element screening. 8
8.1 Principle . 8
8.2 Chemicals, reagents and equipment, including reference materials. 9
8.2.1 Reagents and materials . 9
8.2.2 Apparatus and equipment .10
8.3 Procedure .10
8.3.1 General.10
8.3.2 Safety precautions .10
8.3.3 Pre-treatment .10
8.3.4 Amount of sample .10
8.3.5 Procedure .10
8.4 Interpretation and expression of results .10
8.5 Results .11
8.6 Reporting of data collection.11
9 Test report .11
Annex A (informative) Overview of known GM cotton events .12
Annex B (informative) Overview of detection methods applied by RIKILT .16
Annex C (informative) In-house validation RIKILT .18
Annex D (informative) Workshop contributors .27
Bibliography .30
iv © ISO 2019 – All rights reserved
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
International Workshop Agreement IWA 32 was approved at a workshop hosted by the Netherlands
Standardization Institute (NEN), in association with the Organic Cotton Accelerator, held in New Delhi,
India, in January 2019.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
Introduction
0.1 General
This purpose of this document is to provide guidance to laboratories worldwide to assess, in a
standardized way, whether cotton, cotton fibre and/or cotton-derived materials are produced from, or
contain materials from, genetically modified (GM) cotton plants. This document is intended for non-GM
cotton and textiles production lines, but it can be applied to any production line that wants to check the
presence of GM cotton.
0.2 Protocol
The GM screening protocol described in this document is based on Polymerase Chain Reaction (PCR)-
based methods, as these methods are the minimal set of DNA-based methods to cover all know
...
INTERNATIONAL IWA
WORKSHOP 32
AGREEMENT
First edition
2019-04
Screening of genetically modified
organisms (GMOs) in cotton and
textiles
Criblage pour la détection des organismes génétiquement modifiés
(OGM) dans le coton et les textiles
Reference number
©
ISO 2019
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Sample preparation . 2
6 DNA isolation . 3
6.1 General . 3
6.2 Principle . 3
6.3 Chemicals, reagents and equipment. 4
6.3.1 Reagents . 4
6.3.2 Apparatus and equipment . 4
6.4 Procedure . 5
6.4.1 General. 5
6.4.2 Protocol . 5
6.5 Results . 6
6.5.1 Analysis . 6
7 DNA quality control . 6
7.1 General . 6
7.2 Principle . 6
7.3 Chemicals, reagents and equipment, including reference materials. 6
7.3.1 Reagents . 6
7.3.2 Apparatus and equipment . 7
7.4 Procedure . 8
7.4.1 General. 8
7.4.2 Safety precautions . 8
7.4.3 Pre-treatment . 8
7.4.4 Amount of sample . 8
7.4.5 Procedure . 8
7.5 Results . 8
7.5.1 Calculations . 8
7.5.2 Interpretation and expression of results . 8
7.5.3 Results . 8
8 GM element screening. 8
8.1 Principle . 8
8.2 Chemicals, reagents and equipment, including reference materials. 9
8.2.1 Reagents and materials . 9
8.2.2 Apparatus and equipment .10
8.3 Procedure .10
8.3.1 General.10
8.3.2 Safety precautions .10
8.3.3 Pre-treatment .10
8.3.4 Amount of sample .10
8.3.5 Procedure .10
8.4 Interpretation and expression of results .10
8.5 Results .11
8.6 Reporting of data collection.11
9 Test report .11
Annex A (informative) Overview of known GM cotton events .12
Annex B (informative) Overview of detection methods applied by RIKILT .16
Annex C (informative) In-house validation RIKILT .18
Annex D (informative) Workshop contributors .27
Bibliography .30
iv © ISO 2019 – All rights reserved
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
International Workshop Agreement IWA 32 was approved at a workshop hosted by the Netherlands
Standardization Institute (NEN), in association with the Organic Cotton Accelerator, held in New Delhi,
India, in January 2019.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
Introduction
0.1 General
This purpose of this document is to provide guidance to laboratories worldwide to assess, in a
standardized way, whether cotton, cotton fibre and/or cotton-derived materials are produced from, or
contain materials from, genetically modified (GM) cotton plants. This document is intended for non-GM
cotton and textiles production lines, but it can be applied to any production line that wants to check the
presence of GM cotton.
0.2 Protocol
The GM screening protocol described in this document is based on Polymerase Chain Reaction (PCR)-
based methods, as these methods are the minimal set of DNA-based methods to cover all know
...
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