This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISOÂ 4387.
NOTEÂ Â Â Â Â Â ISOÂ 20778 and ISOÂ 22253 provide the determination method of nicotine in smoke with an intense smoking regime.

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This document specifies methods for the determination of total particulate matter and for the subsequent determination of nicotine-free dry particulate matter present in the smoke from cigarettes generated and collected using a routine analytical smoking machine.

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ISO/IEC 17025:2017 specifies the general requirements for the competence, impartiality and consistent operation of laboratories.
ISO/IEC 17025:2017 is applicable to all organizations performing laboratory activities, regardless of the number of personnel.
Laboratory customers, regulatory authorities, organizations and schemes using peer-assessment, accreditation bodies, and others use ISO/IEC 17025:2017 in confirming or recognizing the competence of laboratories.

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This International Standard specifies two methods of providing representative samples of a population of cigarettes manufactured for sale. Different procedures are specified (see Table 1) according to whether sampling is undertaken at the point of sale or at a factory.
a) Sampling “at one point in time” provides for appraisal of the chosen properties of the cigarettes on that occasion. Sampling is carried out within as short a period as possible.
b) Sampling “over a period of time” provides for on-going appraisals. It can be considered for practical purposes as a series of samples each taken “at one point in time”.

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This International Standard: - defines smoking parameters and specifies the standard conditions to be provided for the routine analytical machine smoking of cigarettes; - specifies the requirements for a routine analytical smoking machine complying with the standard conditions. Annex A specifies the ambient air velocities surrounding cigarettes in a routine analytical smoking machine, the mechanical design of the enclosures immediately surrounding them, and the methods of air velocity measurement including the location where air velocity is measured. Annex B describes, as an example, the special characteristics of a typical smoking machine incorporating a piston type of puffing mechanism. Annex C includes a diagram of a puff profile and illustrates definitions and standard conditions.

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This International Standard specifies a method for the determination of carbon monoxide (CO) in the vapour phase of cigarette smoke.

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