CEN/TC 332 - Laboratory equipment

This document provides guidance and establishes requirements for collecting and examining volumetric performance data of automated liquid handling systems (ALHS). It specifies how to index and track volumetric performance data and provides descriptive statistics for the evaluation of these data. This document also specifies reporting requirements of ALHS volumetric performance.
This document is applicable to all ALHS with complete, installed liquid handling devices, including tips and other essential parts needed for delivering a specified volume, which perform liquid handling tasks without human intervention into labware.
NOTE       For terminology and general requirements of automated liquid handling systems, see ISO 23783-1. Measurement procedures for the determination of volumetric performance are given in ISO 23783-2.

This document defines terms relating to automated liquid handling systems (ALHS). This document also specifies general requirements for the use of ALHS. It describes types of ALHS and specific use requirements, settings, and adjustments for each ALHS type. It also specifies environmental requirements for the use of ALHS.
This document is applicable to all ALHS with complete, installed liquid handling devices, including tips and other essential parts needed for delivering a specified volume, which perform liquid handling tasks without human intervention into labware.
NOTE       Measurement procedures for the determination of volumetric performance are given in ISO 23783-2. The determination, specification, and reporting of volumetric performance of automated liquid handling systems are described in ISO 23783-3.

This document specifies procedures for the determination of volumetric performance of automated liquid handling systems (ALHS), including traceability and estimations of measurement uncertainty of measurement results.
This document is applicable to all ALHS with complete, installed liquid handling devices, including tips and other essential parts needed for delivering a specified volume, which perform liquid handling tasks without human intervention into labware.
NOTE       For terminology and general requirements of automated liquid handling systems, see ISO 23783-1. Determination, specification, and reporting of volumetric performance of automated liquid handling systems is described in ISO 23783-3.

This document provides terms and definitions for micro process engineering and microfluidics applied in medical and veterinary diagnostics, chemistry, agriculture, pharmacy, biotechnology and the agrifood industry, as well as other application areas.

This document is a product specification, giving performance requirements for fire safety storage cabinets to be used for the storage of flammable liquids. It is applicable to cabinets with a total internal volume of not greater than 2 m3, which can be free standing, restrained to a wall or mounted on plinth or castors.
This document does not apply to brick enclosures or walk-in storage rooms.
This document does not apply to any support frame or mechanism other than the base which is integral to the fire safety storage cabinet.
Requirements are given in respect of the construction of the fire safety storage cabinet and its capacity to resist fire conditions on the outside. A classification of fire safety storage cabinets is given, according to the level of fire resistance offered, and a type test is included, see Annex A.
The tests described in this document are type tests.
This document does not discriminate between different flammable liquids, which can have considerably different physical properties.
Attention is drawn to national regulations, which can apply with regard to the storage of flammable liquids.

This document specifies requirements and tests for glass Petri dishes intended for general laboratory purposes and microbiological work.

This document specifies requirements for borosilicate 3,3 glass tubing according to ISO 3585 for laboratory apparatus in an outer diameter range from 4 mm to 300 mm. This document defines dimensions, material, denomination, designation, requirements and inspection methods.

This document specifies the characteristics of fume cupboards, as defined in EN 14175-1, for work with unsealed radioactive materials with specific requirements regarding radiation protection. It does not apply to fume cupboards, glove boxes or hot cells (shielded radiation containment cells which can incorporate fume extraction).
The purpose of this document is to set out rules for the design and testing of fume cupboards for work with unsealed radioactive materials, in order to provide guidelines for the manufacturer, planner, installer, operator, assessor and the authorities.
This document only covers bench type fume cupboards.

This document specifies requirements for the seamless integration with other microfluidic components and systems to facilitate the process of designing new microfluidic devices (e.g. microfluidic chips, sensors, actuators, connectors).
This document is applicable to devices in the field of “microfluidics” needing microfluidic interconnections.

This document applies to an articulated extraction arm used as a local exhaust device in laboratories and comprised of a capture device (receiving, enclosing or capture hood, nozzle or flat screen) connected to an extraction arm which is articulated ducting to move air from the capture device to discharge.
This standard specifies:
- a method for type testing;
- a method to assess the three-dimensional capture zone of local exhaust devices mounted on an articulated extract arm;
- a method for assessing the emission release capture efficiency of local exhaust devices connected to an articulated extract arm and its robustness to a challenge of air disturbance directly in front of and in close proximity to the capture hood and release source;
- a method for establishing the reachable, three-dimensional workspace of local exhaust devices mounted on an articulated extract arm by measuring the possible positions of the opening of the device;
- a method for measuring the pressure drop and noise level in the type test;
- instructions for marking the device and recommended content of information for use;
- guidance for use describing the limitations of local exhaust devices with articulated extract arm for different airflow rates establishing the capture zone;
- guidance on selection, installation, commissioning, and control testing of articulated extract arms and their local exhaust ventilation systems.
The scope does not include filtration requirements and impact of fully or partly recirculation of the airflow extracted by an articulated extract arm.

This document specifies
—    metrological requirements,
—    maximum permissible errors,
—    requirements for marking and
—    information to be provided for users,
for manually operated precision laboratory syringes made of glass or glass and metal designed to deliver their selected volume (Ex).
Manually operated precision laboratory syringes are instruments used for delivering liquids and gases. The barrel is typically made of glass and the plunger and the needle are typically made of metal.

This document specifies general requirements for piston-operated volumetric apparatus (POVA). It is applicable to pipettes, burettes, dilutors, dispensers and manually operated precision laboratory syringes. It furthermore defines terms for the use of piston-operated volumetric apparatus and gives user recommendations.
This document does not apply to medical products intended for use on humans, e.g. for medical syringes.

This document specifies
—    metrological requirements,
—    maximum permissible errors,
—    requirements for marking and
—    information to be provided for users,
for dilutors with a sample uptake capacity (In) from 5 μl to 1 ml and a diluent capacity (Ex) from 50 μl to 100 ml. They are designed to deliver the sample and diluent together in measured proportion and measured volume.

This document specifies alternative measurement procedures for the determination of  volume of piston-operated volumetric apparatus.
The procedures are applicable to complete systems comprising the basic apparatus and all parts selected for use with the apparatus, disposable or reusable, involved in the measurement by delivery process (Ex). Methods described in this document are suitable for various maximum nominal volumes of piston-operated volumetric apparatus. It is the responsibility of the user to select the appropriate method.

This document specifies
—    metrological requirements,
—    maximum permissible errors,
—    requirements for marking and
—    information to be provided for users,
for air-displacement (type A) and positive displacement (type D) single-channel and multi-channel pipettes, complete with their selected tip(s) and any other essential, consumable parts, designed to deliver the selected volume (Ex).

This document specifies
—    metrological requirements,
—    maximum permissible errors,
—    requirements for marking and
—    information to be provided for users,
for dispensers. It is applicable to dispensers with nominal volumes from 1 μl up to 200 ml, designed to deliver their volume (Ex).

This document specifies
—    metrological requirements,
—    maximum permissible errors,
—    requirements for marking and
—    information to be provided for users,
for burettes. This document is applicable to burettes with nominal volumes up to 100 ml, designed to deliver their specified volume (Ex).

This document specifies a gravimetric reference measurement procedure for the determination of volume of piston-operated volumetric apparatus (POVA). The procedure is applicable to complete systems comprising the basic apparatus and all parts selected for use with the apparatus, disposable or reusable, involved in the measurement by delivery (Ex) or contained (In).

This document specifies the photometric reference measurement procedure for the determination of volume of piston-operated volumetric apparatus (POVA). The procedure is applicable to complete systems comprising the basic apparatus with a maximum nominal volume of 5 000 µl and all parts selected for use with the apparatus, disposable or reusable, involved in the measurement by delivery (Ex).

This document provides methods for the testing, calibration and use of volumetric instruments made from glass and plastic in order to obtain the best accuracy in use.
NOTE      Testing is the process by which the conformity of the individual volumetric instrument with the appropriate standard is determined, resulting in the determination of its error of measurement at one or more points.
This document is applicable to volumetric instruments with nominal capacities in the range of 100 µl to 10 000 ml. These include single-volume pipettes (see ISO 648), graduated pipettes (see ISO 835), burettes (see ISO 385), volumetric flasks (see ISO 1042 and ISO 5215), and graduated measuring cylinders (see ISO 4788 and ISO 6706).
The methods are not intended for testing of volumetric instruments with capacities below 100 µl such as micro-glassware.
This document does not deal specifically with pycnometers as specified in ISO 3507. However, the procedures specified for the determination of volume of glassware can, for the most part, also be followed for the determination of a pycnometer volume. For some types of pycnometers, special handling can be necessary.

This document is a product specification, giving performance requirements for water overhead emergency safety body showers installed on industrial and logistic sites, (in combination with safety eyewashes and hand-held showers as well),
a)   which are permanently connected to a water supply; or
b)   which are equipped with a store tank and optionally connected to an uninterrupted or a temporary water supply.
Emergency safety body showers using fluid other than water are not considered in this document
This document also specifies requirements in respect of installation, adjustment and marking of the showers as well as operation and maintenance instructions to be given by the manufacturer.
NOTE 1   Plumbed-in body showers designed for laboratory facilities are dealt with in EN 15154-1.
NOTE 2   Water multiple nozzle body showers for sites other than laboratories are dealt with in prEN 15154-6.
NOTE 3   Attention is drawn to national regulations which can apply in respect of the installation and use of emergency safety showers

This document applies for the planning, design, installation and commissioning of ventilation systems in laboratories. It also applies for scientific classrooms in schools when equipped with a ventilation system.
The application of this document depends not on the term laboratory in its narrower sense but this document also applies also for laboratory-related rooms in which work with dangerous or health hazardous substances is performed.

This document is a product specification, giving performance requirements for plumbed-in multiple nozzle emergency safety body showers which are permanently connected to a water supply and installed on industrial and logistic sites.
Emergency safety body showers using fluid other than water are not considered in this document.
This document also specifies requirements in respect of installation, adjustment and marking of the showers as well as operation and maintenance instructions to be given by the manufacturer.
NOTE 1   Plumbed-in emergency safety body showers designed for laboratory facilities are dealt with in EN 15154-1.
NOTE 2   Water overhead body showers for sites other than laboratories are dealt with in FprEN 15154-5.
NOTE 3   Attention is drawn to national regulations which can apply in respect of the installation and use of emergency safety showers.

This document specifies type test methods for the assessment of safety and performance of fume cupboards connected to an exhaust air system. Relevant requirements are specified in EN 14175-2.
For terms and their definitions, EN 14175-1 applies. For safety and performance requirements of fume cupboards, EN 14175-2 applies. For on-site test methods of fume cupboards, EN 14175-4 applies. For the type testing and on-site testing of variable air volume (VAV) fume cupboards, EN 14175-6 applies in addition to this standard. For fume cupboards for high heat and acidic load, EN 14175-7 applies.
For the testing of recirculation filtration fume cupboards, EN 17242:-  applies.
For the testing of microbiological safety cabinets, EN 12469 applies.

ISO 16496:2016 recommends dimensions and specifies requirements and test methods for laboratory glassware manufactured from borosilicate glass 3.3 and provided with a vacuum jacket for thermal insulation. It covers Dewar vessels, vacuum-jacketed reaction vessels and vacuum-jacketed columns intended for laboratory use and laboratory related applications. Typical dimensions are given in Tables 1 to 5.
ISO 16496:2016 does not apply to large scale production equipment and equipment operated with pressures of more than 0,1 bar above atmospheric pressure.

ISO 4796-1:2016 specifies a series of screw-neck bottles suitable for the storage of fluid liquid and solid chemicals and reagents in general laboratory use. These bottles with nominal volumes ranging from 25 ml to 20 000 ml are also suitable for the preparation and storage of microbiological growth media.

ISO 384:2015 sets out principles for the design of volumetric instruments manufactured from glass or from plastics in order to facilitate the most reliable and convenient use to the intended degree of accuracy.

ISO 4797:2015 specifies requirements for an internationally acceptable series of boiling flasks with conical ground joints for general laboratory purposes.

ISO 3819:2015 specifies requirements for an internationally acceptable series of glass beakers for laboratory use.

ISO 6556:2012 specifies requirements to filter flasks with conical or cylindrical shape for general laboratory purposes.

This document applies for
- fume cupboards for high heat load
- fume cupboards for handling of perchloric acid
- fume cupboards for handling of hydrofluoric acid
- fume cupboards for high heat load in combination with acidic digestions.
This document applies in conjunction with EN 14175-1 to EN 14175-4 and, where appropriate, to EN 14175-6 and specifies supplementary information relevant to vocabulary, safety and performance requirements, type test methods, on-site test methods and marking of the listed special purpose fume cupboards.
NOTE   EN 14175-6 applies for variable air volume fume cupboards. Experience shows that fume cupboards for high heat  load offer much safer working conditions when operated with fixed air volume flow.
This document does not apply for microbiological safety cabinets, recirculatory filtration fume cupboards and fume cupboards for carrying out work on radioactive materials.

ISO 13079:2011 specifies requirements for single-use and re-usable glass and plastics tubes for measuring the erythrocyte sedimentation rate (ESR) by the Westergren method, and for a support to hold tubes during the performance of the test. These so-called "Westergren tubes" are also sometimes designated as "Westergren pipettes". A procedure for measuring the erythrocyte sedimentation rate by the Westergren method is given in an informative annex.
ISO 13079:2011 does not apply to single-use containers for human venous blood specimen collection and their accessories for which other standards apply. It also does not apply for devices where the Westergren method has been used as basis to develop other, similar methods or equipment for the erythrocyte sedimentation rate determination.

ISO 13130:2011 specifies requirements and tests for desiccators and vacuum desiccators intended for general laboratory purposes such as drying of substances or material.

ISO 24998:2008 specifies requirements and test methods for plain, single-use Petri dishes for microbiological use.
ISO 24998:2008 does not apply to products of similar design which may be used for cell or tissue culture purposes. Neither does it apply to dishes supplied ready loaded with microbiological media.

ISO 648:2008 specifies metrological and constructional requirements for volumetric pipettes with one mark (total delivery) and for volumetric pipettes with two marks, both of which are adequate for general laboratory purposes.
The details specified are in conformity with the principles of design and construction of volumetric glassware given in ISO 384.

This European Standard is a product specification, giving minimum performance requirements for non plumbed-in emergency safety body showers.
It is applicable to body showers filled with a washing fluid by the manufacturer and to empty devices to be filled prior to putting into service. Both variations are for first aid use when the body or parts of the body have been exposed to harmful substances or heat.
Requirements are also given concerning labelling, marking and information to be supplied by the manufacturer.
Throughout this standard, the term non plumbed-in emergency safety body shower is referred to as body shower.

This European Standard is a product specification, giving minimum performance requirements for non plumbed-in emergency safety eyewash units.
It is applicable to non plumbed-in emergency safety eyewash units filled with a rinsing fluid by the manufacturer for first aid use when the eyes have been exposed to harmful substances.
Requirements are also given concerning labelling, marking and information to be supplied by the manufacturer.
Throughout this standard, the term “non plumbed-in emergency safety eyewash unit” is referred to as "eyewash unit".

TC - Modifications in Clauses 2, 3, 6, C.1 and C.2.

TC - Modifications in Clause 2 and Subclause 6.1.

ISO 835:2007 specifies metrological and constructional requirements for graduated pipettes, adequate for general laboratory purposes.

This document is a product specification, giving performance requirements for emergency safety body showers connected to the water supply. It is applicable to plumbed-in body showers only, located in laboratory facilities. It is not applicable to emergency safety showers used on industrial sites or in other such areas.
Requirements are given in respect of the performance, installation, adjustment and marking of the showers as well as installation, operation and maintenance instructions to be given by the manufacturer.
NOTE   Attention is drawn to national regulations which may apply in respect of the installation and use of emergency safety showers.

This document is a product specification, giving performance requirements for emergency safety eye wash units connected to the water supply. It is applicable to plumbed-in eye wash units only.
Requirements are given in respect of the performance, installation, adjustment and marking of the eye wash units, as well as installation, operation and maintenance instructions to be given by the manufacturer.
NOTE   Attention is drawn to national regulations which may apply in respect of the installation and use of eye wash units.

This European standard is a product specification, giving performance requirements for fire safety cabinets used for storing pressurised gas cylinders. It is applicable to cabinets with a total internal volume suitable to store pressurised gas cylinders with a total volume not exceeding 220 l, including cylinders of purging gases.
NOTE 1   This means that up to four gas cylinders of 50 l or up to three gas cylinders of 70 l can be stored in a single cabinet.
NOTE 2   It is intended that the pressurised gas cylinders can be in use while in the cabinet.
NOTE 3   Attention is drawn to national regulations which can apply with regard to the storage and use of pressurised gas cylinders.
NOTE 4   The safety cabinet can be free standing, restrained to a wall or mounted on wheels or castors.
This standard is not applicable to brick enclosures, walk-in storage rooms or cabinets which do not take their weight on their base.
Requirements are given in respect to the construction of the cabinet and its capacity to resist fire conditions on the outside. A type test is included, which is based on the already existing fire resistance (heating curve) tests given in EN 14470-1.
The tests described in this European Standard are type tests (for the storage of flammable liquids EN 14470-1 is applicable – the criteria for failure are different – see Annex A).

This document specifies requirements and type test methods for VAV systems. It also specifies additional requirements to those in EN 14175-2 and additional test methods to those in EN 14175-3 and EN 14175-4 for fume cupboards with VAV systems (VAV fume cupboards).
For terms and definitions, EN 14175-1 applies. For safety and performance requirements of fume cupboards, EN 14175-2 applies. For type testing of fume cupboards with preset air volume flow, EN 14175-3 applies. For on-site test methods of fume cupboards already installed in a laboratory, EN 14175-4 applies.

This Technical Specification specifies a selection of recommendations for the installation and maintenance of fume cupboards in accordance with EN 14175-2 and EN 14175-6. The maintenance recommendations can be applicable to other fume cupboards as well.

ISO 24450:2005 specifies requirements and dimensions for an internationally acceptable series of conical flasks and of flat-bottom and round-bottom flasks with wide neck for general laboratory purpose. The flasks are provided for direct use in laboratory, fitting together with other equipment for general laboratory purposes, and for further work up to other products.
For narrow-necked boiling flasks, ISO 1773 applies. For boiling flasks with conical ground joints, see ISO 4797.

ISO 4788:2005 specifies dimensions, material and constructional and metrological requirements of graduated measuring cylinders of tall form (Type 1a and Type 1b) and of squat form (Type 2). All types are suitable for general laboratory use.
The specifications in ISO 4788:2005 are in conformity with the principles of design and construction of volumetric glassware given in ISO 384.

This International Standard provides metrological and construction requirements for an internationally acceptable series of burettes, suitable for general laboratory purposes.
The details specified are in accordance with the principles of design and construction of volumetric glassware given in ISO 384.
For piston burettes, see ISO 8655-3.

This Part 4 of the European Standard specifies a selection of on-site test methods for the following fume cupboards:
- Fume cupboards designed in accordance with Part 2 of this European Standard and type tested in accordance with Part 3 of this European Standard.
- Fume cupboards designed in accordance with Part 2 of this European Standard and not type tested.
- General purpose fume cupboards designed in accordance with Part 2 of this European Standard which are used under  customer-specific conditions, such as for the handling of very toxic substancies or bulky load of the work space.
The test methods are designed to be used at the place of installation of the fume cupboard, usually a laboratory. They are used for commissioning after installation, maintenance and qualification purposes.

This part 1 of EN 14175 provides terms and definitions for fume cupboards (see 3.1). Corresponding terms in eight European languages are given in the normative annex A.
For safety and performance requirements of fume cupboards EN 14175-2 applies.
For type testing of fume cupboards EN 14175-3 applies.
For microbiological safety cabinets EN 12469 applies.
This part of EN 14175 does not address recirculatory filtration fume cupboards or devices used as animal accomodation.