EN ISO 6887-1:1999

Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:1999)

This standard gives general guidance for the preparation of dilutions for microbiological examination.

Mikrobiologie von Lebensmitteln und Futtermitteln - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen und Dezimalverdünnungen (ISO 6887-1:1999)

Dieser Teil von ISO 6887 legt allgemeine Regeln für die aerobe Herstellung von Erstverdünnungen und Dezimalverdünnungen für mikrobiologische Untersuchungen von Lebensmitteln und Futtermitteln fest. Dieser Teil von ISO 6887 ist im allgemeinen anwendbar, außer auf die in ISO 6887-2 gennanten Produkte.

Microbiologie des aliments - Préparation des échantillons de la suspension mère et des dilutions en vue de l'examen microbiologique - Partie 1: Règles générales pour la préparation de la suspension mère et des dilutions décimales (ISO 6887-1:1999)

Mikrobiologija živil in krmil - Priprava vzorcev za preskušanje ter osnovne in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna pravila za pripravo osnovne in decimalnih razredčin (ISO 6887-1:1999)

General Information

• Status: Withdrawn
• Publication Date: 14-Feb-1999
• Withdrawal Date: 04-Apr-2017
• ICS: 07.100 - Microbiology
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 6 - Microbiological contamination
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Completion Date: 05-Apr-2017
• Ref Project: SIST EN ISO 6887-1:1999 - Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:1999)

Relations

• Replaced By: EN ISO 6887-1:2017 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
• Effective Date: 12-Apr-2017

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-1999
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Microbiology of food and animal feeding stuffs - Preparation of test samples, initial
suspension and decimal dilutions for microbiological examination - Part 1: General rules
for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:1999)
Mikrobiologie von Lebensmitteln und Futtermitteln - Vorbereitung von
Untersuchungsproben und Herstellung von Erstverdünnungen und von
Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln
für die Herstellung von Erstverdünnungen und Dezimalverdünnungen (ISO 6887-1:1999)
Microbiologie des aliments - Préparation des échantillons de la suspension mere et des
dilutions en vue de l'examen microbiologique - Partie 1: Regles générales pour la
préparation de la suspension mere et des dilutions décimales (ISO 6887-1:1999)
Ta slovenski standard je istoveten z: EN ISO 6887-1:1999
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

INTERNATIONAL ISO
STANDARD 6887-1
First edition
1999-02-15
Microbiology of food and animal feeding
stuffs — Preparation of test samples, initial
suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial
suspension and decimal dilutions
Microbiologie des aliments — Préparation des échantillons, de la
suspension mère et des dilutions décimales en vue de l'examen
microbiologique —
Partie 1: Règles générales pour la préparation de la suspension mère et
des dilutions décimales
A
Reference number
ISO 6887-1:1999(E)
ISO 6887-1:1999(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
International Standard ISO 6887-1 was prepared by Technical Committee ISO/TC 34, Agricultural food products,
Subcommittee SC 9, Microbiology.
This first edition of ISO 6887-1 cancels and replaces ISO 6887:1983.
ISO 6887 consists of the following parts, under the general title Microbiology of food and animal feeding stuffs —
Preparation of test samples, initial suspension and decimal dilutions for microbiological examination:
 Part 1: General rules for the preparation of the initial suspension and decimal dilutions for microbiological
examination
 Part 2: Specific rules for the preparation of test samples and initial suspension
Part 2 will probably be divided into several parts, for specific products such as meat, milk, fish and other products.
©  ISO 1999
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic
or mechanical, including photocopying and microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 • CH-1211 Genève 20 • Switzerland
Internet [e-mail]
Printed in Switzerland
ii
© ISO
ISO 6887-1:1999(E)
Introduction
Because of the large variety of food and feed products, this horizontal method may not be appropriate in every
detail for certain products. In this case, different methods, which are specific to these products may be used if
absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this
horizontal method as far as possible.
When this part of ISO 6887 is next reviewed, account will be taken of all information then available regarding the
extent to which this horizontal method has been followed and the reasons for deviations from this method in the
case of particular products.
The harmonization of test methods cannot be immediate, and for certain group of products International Standards
and/or national standards may already exist that do not comply with this horizontal method. It is hoped that when
such standards are reviewed they will be changed to comply with this part of ISO 6887 so that eventually the only
remaining departures from this horizontal method will be those necessary for well-established technical reasons.
This part of ISO 6887 defines the general rules for the preparation of the initial suspension and of decimal dilutions
for microbiological examination. Part 2 of ISO 6887 (under preparation) will specify specific rules for the preparation
of the test sample and of the initial suspension, taking into account the variety of food and feed products to which
ISO 6887 applies.
For a number of products, it is necessary to take special precautions especially when preparing the initial
suspension, because of the physical state of the product (such as a dry product, a highly viscous product), or the
presence of inhibitory substances (such as spices, salted fishes), or the acidity, etc.
It is recommended that, whilst waiting for the publication of part 2, any special diluents or practices specified for
particular products in an appropriate specific standard be used in the preparation of the initial suspension. This may
include:
 adjustment of the pH of a food suspension to neutrality;
 the use of buffered peptone water, and no other diluent, for products with high inhibitory effect, or products
containing microorganisms that have been stressed (e.g. acidic pH);
 specific rehydration procedures for foods of low water activity to minimize osmotic shock;
 the use of adequate temperatures to aid suspension of cocoa, gelatine, milk powder, etc.;
 resuscitation procedures for the improved recovery of stressed microorganisms resulting from food processing
and storage;
 homogenization procedures and duration specific to certain products (e.g. cereals) and/or to certain
determinations (e.g. yeasts and moulds);
 the use of surface-active agents for high-fat foods.
iii
INTERNATIONAL STANDARD  © ISO ISO 6887-1:1999(E)
Microbiology of food and animal feeding stuffs — Preparation of
test samples, initial suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial suspension and decimal
dilutions
1 Scope
This part of ISO 6887 defines general rules for the aerobic preparation of the initial suspension and of decimal
dilutions for microbiological examinations of products intended for human or animal consumption.
This part of ISO 6887 is applicable to the general case, except for products mentioned in ISO 6887-2.
2 Normative reference
The following normative document contains provisions which, through reference in this text, constitute provisions of
this part of ISO 6887. For dated references, subsequent amendments to, or revisions of, this publication do not
apply. However, parties to agreements based on this part of ISO 6887 are encouraged to investigate the possibility
of applying the most recent editions of the normative documents indicated below. For undated references, the latest
edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently valid
International Standards.
ISO 7218, Microbiology of food and animal feeding stuffs — General rules for microbiological examinations.
3 Definitions
For the purposes of this part of ISO 6887, the following definitions apply.
3.1
initial suspension (primary dilution)
suspension, solution or emulsion obtained after a weighed or measured quantity of the product under examination
(or of a test sample prepared from the product) has been mixed with a nine-fold quantity of diluent, allowing large
p
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