SIST-TS CEN/TS 15634-4:2016

Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR

This Technical Specification describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [1], [2].

Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen Verfahren - Teil 4: Erdnuss (Arachis hypogaea) - Qualitativer Nachweis einer spezifischen DNA-Sequenz in Schokolade mittels Real-time PCR

Diese Technische Spezifikation beschreibt ein Verfahren zum qualitativen Nachweis von Erdnuss (Arachis hypogaea) in Schokolade mittels Real-time PCR auf der Basis des Gens für das Erdnussallergen Ara h 2 [4], [5].

Produits alimentaires - Détection d’allergènes alimentaires par des méthodes de biologie moléculaire - Partie 4 : Arachide (Arachis hypogaea) - Détection qualitative d’une séquence d’ADN spécifique dans du chocolat, par PCR en temps réel

Živila - Odkrivanje prisotnosti alergenov v živilih z metodami molekularne biologije - 4. del: Arašidi (Arachis hypogaea) - Kvalitativno odkrivanje specifičnega niza DNK v čokoladi s PCR v realnem času

Ta tehnična specifikacije opisuje postopek za kvalitativno odkrivanje arašidov (Arachis hypogaea) v čokoladi s PCR v realnem času na podlagi gena za alergen iz arašida Ara h 2 [1], [2].

General Information

• Status: Withdrawn
• Public Enquiry End Date: 30-Dec-2015
• Publication Date: 19-Apr-2016
• Withdrawal Date: 05-Apr-2023
• ICS: 07.100.30 - Food microbiology
• ICS: 67.050 - General methods of tests and analysis for food products
• ICS: 67.190 - Chocolate
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 29-Mar-2023
• Due Date: 21-Apr-2023
• Completion Date: 06-Apr-2023
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Ref Project: CEN/TS 15634-4:2016 - Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR

Relations

• Revises: SIST EN 15634-4:2023 - Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
• Effective Date: 01-May-2023

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 15634-4:2016
01-maj-2016
äLYLOD2GNULYDQMHSULVRWQRVWLDOHUJHQRYYåLYLOLK]PHWRGDPLPROHNXODUQHELRORJLMH
GHO$UDãLGL$UDFKLVK\SRJDHD.YDOLWDWLYQRRGNULYDQMHVSHFLILþQHJDQL]D
'1.YþRNRODGLV3&5YUHDOQHPþDVX
Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut
(Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by
real-time PCR
Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen
Verfahren - Teil 4: Erdnuss (Arachis hypogaea) - Qualitativer Nachweis einer
spezifischen DNA-Sequenz in Schokolade mittels Real-time PCR
Produits alimentaires - Détection d’allergènes alimentaires par des méthodes de biologie
moléculaire - Partie 4 : Arachide (Arachis hypogaea) - Détection qualitative d’une
séquence d’ADN spécifique dans du chocolat, par PCR en temps réel
Ta slovenski standard je istoveten z: CEN/TS 15634-4:2016
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.190 ýRNRODGD Chocolate
SIST-TS CEN/TS 15634-4:2016 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST-TS CEN/TS 15634-4:2016

---------------------- Page: 2 ----------------------

SIST-TS CEN/TS 15634-4:2016


CEN/TS 15634-4
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

March 2016
TECHNISCHE SPEZIFIKATION
ICS 07.100.30; 67.190
English Version

Foodstuffs - Detection of food allergens by molecular
biological methods - Part 4: Peanut (Arachis hypogaea) -
Qualitative detection of a specific DNA sequence in
chocolate by real-time PCR
Produits alimentaires - Détection d'allergènes Lebensmittel - Nachweis von Lebensmittelallergenen
alimentaires par des méthodes de biologie moléculaire mit molekularbiologischen Verfahren - Teil 4: Erdnuss
- Partie 4 : Arachide (Arachis hypogaea) - Détection (Arachis hypogaea) - Qualitativer Nachweis einer
qualitative d'une séquence d'ADN spécifique dans du spezifischen DNA-Sequenz in Schokolade mittels Real-
chocolat, par PCR en temps réel time PCR
This Technical Specification (CEN/TS) was approved by CEN on 11 February 2016 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 15634-4:2016 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST-TS CEN/TS 15634-4:2016
CEN/TS 15634-4:2016 (E)
Contents Page
European foreword . 3
1 Scope . 4
2 Principle . 4
3 Reagents . 4
3.1 DNA extraction with CTAB . 4
3.2 DNA purification by means of solid phase extraction . 5
3.3 Real-time PCR reagents .
...