Leather - Chemical determination of formaldehyde content - Part 1: Method using high-performance liquid chromatography (ISO 17226-1:2021)

This document specifies a method for the determination of free and released formaldehyde in leathers.
This method, based on high-performance liquid chromatography (HPLC), is selective and not sensitive
to coloured extracts and is intended to be used for precise quantification of formaldehyde.
The formaldehyde content is taken to be the quantity of free formaldehyde and formaldehyde extracted
through hydrolysis contained in a water extract from the leather under standard conditions of use.

Leder - Chemische Bestimmung des Formaldehydgehalts - Teil 1: Verfahren mittels Flüssigkeitschromatographie (ISO 17226-1:2021)

Dieses Dokument legt ein Verfahren zur Bestimmung von freiem und freigesetztem Formaldehyd in Leder fest. Dieses Verfahren, das auf Hochleistungsflüssigkeitschromatographie (HPLC, en: high-performance liquid chromatography) beruht, ist selektiv und unempfindlich gegenüber gefärbten Extrakten und dient der präzisen Quantifizierung von Formaldehyd.
Der Formaldehydgehalt ist die Menge an freiem Formaldehyd und durch Hydrolyse freigesetztem Formaldehyd, die in einem wässrigen Extrakt, der unter standardisierten Anwendungsbedingungen aus dem Leder erhalten wurde, enthalten ist.

Cuir - Dosage chimique du formaldéhyde - Partie 1: Méthode par chromatographie en phase liquide à haute performance (ISO 17226-1:2021)

Usnje - Kemijsko določevanje formaldehida - 1. del: Metoda s tekočinsko kromatografijo visoke ločljivosti (ISO 17226-1:2021)

General Information

Status
Published
Public Enquiry End Date
01-Jul-2020
Publication Date
24-Mar-2021
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
17-Mar-2021
Due Date
22-May-2021
Completion Date
25-Mar-2021

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SLOVENSKI STANDARD
SIST EN ISO 17226-1:2021
01-maj-2021
Nadomešča:
SIST EN ISO 17226-1:2019
Usnje - Kemijsko določevanje formaldehida - 1. del: Metoda s tekočinsko
kromatografijo visoke ločljivosti (ISO 17226-1:2021)

Leather - Chemical determination of formaldehyde content - Part 1: Method using high-

performance liquid chromatography (ISO 17226-1:2021)
Leder - Chemische Bestimmung des Formaldehydgehalts - Teil 1: Verfahren mittels
Flüssigkeitschromatographie (ISO 17226-1:2021)

Cuir - Dosage chimique du formaldéhyde - Partie 1: Méthode par chromatographie en

phase liquide à haute performance (ISO 17226-1:2021)
Ta slovenski standard je istoveten z: EN ISO 17226-1:2021
ICS:
59.140.30 Usnje in krzno Leather and furs
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
SIST EN ISO 17226-1:2021 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 17226-1:2021
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SIST EN ISO 17226-1:2021
EN ISO 17226-1
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2021
EUROPÄISCHE NORM
ICS 59.140.30 Supersedes EN ISO 17226-1:2019
English Version
Leather - Chemical determination of formaldehyde content
- Part 1: Method using high-performance liquid
chromatography (ISO 17226-1:2021)

Cuir - Dosage chimique du formaldéhyde - Partie 1: Leder - Chemische Bestimmung des

Méthode par chromatographie en phase liquide à haute Formaldehydgehalts - Teil 1: Verfahren mittels

performance (ISO 17226-1:2021) Flüssigkeitschromatographie (ISO 17226-1:2021)
This European Standard was approved by CEN on 12 February 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17226-1:2021 E

worldwide for CEN national Members.
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SIST EN ISO 17226-1:2021
EN ISO 17226-1:2021 (E)
Contents Page

European foreword ....................................................................................................................................................... 3

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SIST EN ISO 17226-1:2021
EN ISO 17226-1:2021 (E)
European foreword

This document (EN ISO 17226-1:2021) has been prepared by Technical Committee IULTCS

"International Union of Leather Technologists and Chemists Societies" in collaboration with Technical

Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by September 2021, and conflicting national standards

shall be withdrawn at the latest by September 2021.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN ISO 17226-1:2019.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,

Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,

Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of

North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the

United Kingdom.
Endorsement notice

The text of ISO 17226-1:2021 has been approved by CEN as EN ISO 17226-1:2021 without any

modification.
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SIST EN ISO 17226-1:2021
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SIST EN ISO 17226-1:2021
INTERNATIONAL ISO
STANDARD 17226-1
IULTCS
IUC 19-1
Third edition
2021-02
Leather — Chemical determination of
formaldehyde content —
Part 1:
Method using high-performance liquid
chromatography
Cuir — Dosage chimique du formaldéhyde —
Partie 1: Méthode par chromatographie en phase liquide à haute
performance
Reference numbers
ISO 17226-1:2021(E)
IULTCS/IUC 19-1:2021(E)
ISO 2021
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SIST EN ISO 17226-1:2021
ISO 17226-1:2021(E)
IULTCS/IUC 19-1:2021(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2021

All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may

be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting

on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address

below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2021 – All rights reserved
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SIST EN ISO 17226-1:2021
ISO 17226-1:2021(E)
IULTCS/IUC 19-1:2021(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Conformity .................................................................................................................................................................................................................. 1

5 Principle ........................................................................................................................................................................................................................ 1

6 Reagents ........................................................................................................................................................................................................................ 2

7 Apparatus ..................................................................................................................................................................................................................... 2

8 Sampling ........................................................................................................................................................................................................................ 3

9 Sample preparation and analysis ........................................................................................................................................................ 3

9.1 Procedure for the determination of formaldehyde in the stock solution ............................................. 3

9.1.1 Preparation of the formaldehyde stock solution .................. ................................................................ 3

9.1.2 Determination .................................................................................................................................................................... 3

9.2 Procedure for the determination of formaldehyde in leather by the HPLC method .................. 4

9.2.1 Extraction of the leather ........................................................................................................................................... . 4

9.2.2 Derivatization with DNPH and determination of formaldehyde in leather

with HPLC .............................................................................................................................................................................. 4

9.2.3 Calibration of HPLC ....................................................................................................................................................... 4

10 Expression of results ........................................................................................................................................................................................ 5

10.1 Calculation of the formaldehyde content in leather samples ......................................................................... 5

10.2 Spiking — determination of recovery rate ..................................................................................................................... 5

10.3 Expression of the results ................................................................................................................................................................ 6

10.4 Precision ....................................................................................................................................................................................................... 6

11 Test report ................................................................................................................................................................................................................... 6

Annex A (informative) Precision: reliability of the HPLC method ......................................................................................... 7

Annex B (informative) HPLC conditions............................................................................................................................................................ 8

Bibliography ................................................................................................................................................................................................................................ 9

© ISO 2021 – All rights reserved iii
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SIST EN ISO 17226-1:2021
ISO 17226-1:2021(E)
IULTCS/IUC 19-1:2021(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www .iso .org/ patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO's adherence to the

World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso .org/

iso/ foreword .html.

This document was prepared by the Chemical Test Commission of the International Union of Leather

Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European

Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, the secretariat of

which is held by UNI, in accordance with the agreement on technical cooperation between ISO and CEN

(Vienna Agreement). This document is technically similar to the Colorimetric Section of the method

IUC 19 which was declared an official method at the IULTCS Delegates meeting on 31st May 2003 in

Cancún, Mexico.

IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to

further the advancement of leather science and technology. IULTCS has three Commissions, which

are responsible for establishing international methods for the sampling and testing of leather. ISO

recognizes IULTCS as an international standardizing body for the preparation of test methods for

leather.

This third edition cancels and replaces the second edition (ISO 17226-1:2018), which has been

technically revised.
The main changes to the previous edition are as follows:
— The listing of reagents in Clause 6 has been reorganised.

— The composition of the dinitrophenylhydrazine (DNPH) solution (6.10) has changed. It no longer

contains concentrated o-phosphoric acid. Under acid conditions some extracted synthetic tanning

agents and resins can continue to release formaldehyde over time, giving incorrect high results.

— With the change in composition of the DNPH solution (6.10), the reaction time limits in the previous

edition are no longer necessary. In 9.2.2 the reaction time and temperature have been increased

to 180 min and 50 °C, respectively. Consequently, the text in 9.2.2, 9.2.3.1 and 9.2.3.2 has been

modified.
— A new Clause 10 has been added.
iv © ISO 2021 – All rights reserved
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SIST EN ISO 17226-1:2021
ISO 17226-1:2021(E)
IULTCS/IUC 19-1:2021(E)

— In Annex A, results of a new collaborative interlaboratory trial are presented.

— Annex B has been technically revised.
A list of all parts in the ISO 17226 series can be fou
...

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