EN ISO 9308-1:2014

Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014)

ISO 9308-1:2014 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growth can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is not suitable for these types of water.
ISO 9308-1:2014 is especially suitable for waters with low bacterial numbers that will cause less than 100 total colonies on chromogenic coliform agar (CCA). These may be drinking water, disinfected pool water, or finished water from drinking water treatment plants.
Some strains of E. coli which are β-D-glucuronidase negative, such as Escherichia coli O157, will not be detected as E. coli. As they are β-D-galactosidase positive, they will appear as coliform bacteria on this chromogenic agar.

Wasserbeschaffenheit - Zählung von Escherichia coli und coliformen Bakterien - Teil 1: Membranfiltrationsverfahren für Wässer mit niedriger Begleitflora (ISO 9308-1:2014)

Dieser Teil von ISO 9308 beschreibt ein Verfahren für die Zählung von Escherichia coli (E. coli) und coliformen Bakterien. Das Verfahren beruht auf einer Membranfiltration, einer anschließenden Kultivierung auf einem chromogenen Nähragar und der Berechnung der Anzahl der Zielorganismen in der Probe. Wegen der geringen Selektivität des Differenzierungsagars kann die Begleitflora eine verlässliche Zählung von E. coli und coliformen Bakterien stören, z. B. in Oberflächenwässern oder oberflächennahen Brunnen. Dieses Verfahren ist für solche Wasserarten nicht geeignet.
Dieser Teil von ISO 9308 eignet sich besonders für die Untersuchung von Wässern mit niedrigen Bakterienzahlen, bei denen insgesamt weniger als 100 Kolonien auf dem Chromogenen Coliformen Agar wachsen. Dies können Trinkwässer, desinfizierte Schwimmbeckenwässer oder fertig aufbereitete Wässer aus Wasseraufbereitungsanlagen sein.
Einige Escherichia coli-Stämme, die β-Glucuronidase-negativ sind, wie z. B. Escherichia coli O157, werden nicht als E. coli bestimmt. Da die β-Galaktosidase-positiv sind, erscheinen sie als coliforme Bakterien.

Qualité de l'eau - Dénombrement des Escherichia coli et des bactéries coliformes - Partie 1: Méthode par filtration sur membrane pour les eaux à faible teneur en bactéries (ISO 9308-1:2014)

L'ISO 9308 :2014 spécifie une méthode de dénombrement des Escherichia coli (E. coli) et des bactéries coliformes. La méthode consiste en une filtration sur membrane, suivie d'une mise en culture dans une gélose chromogène et d'un calcul du nombre des organismes cibles présents dans l'échantillon. La gélose de différenciation étant peu sélective, la flore bactérienne générale peut perturber la fiabilité du dénombrement des E. coli et des bactéries coliformes, par exemple, dans certaines eaux de surface ou certaines eaux de puits peu profonds. Cette méthode ne convient pas à ces types d'eau.
L'ISO 9308 :2014 est particulièrement adaptée aux eaux à faible teneur en bactéries qui produiront moins de 100 colonies totales sur de la gélose chromogène pour le dénombrement des bactéries coliformes. Ces eaux peuvent être l'eau potable, l'eau de piscine désinfectée ou l'eau propre sortant de stations de production d'eau potable.
Certaines souches d'E. coli négatives à la β-D-glucuronidase, telles qu'Escherichia coli O157, ne sont pas détectées en tant qu'E. coli. Comme elles sont positives à la β-D-galactosidase, elles sont considérées comme des bactéries coliformes sur la gélose chromogène pour bactéries coliformes (CCA).

Kakovost vode - Ugotavljanje števila Escherichia coli in koliformnih bakterij - 1. del: Metoda membranske filtracije za vode z majhnim številom spremljajočih bakterij (ISO 9308-1:2014)

Ta del ISO 9308 določa metodo za ugotavljanje števila Escherichia coli (E. coli) in koliformnih bakterij. Metoda temelji na membranski filtraciji, posledični kulturi na kromogenem koliformnem agarskem gojišču in izračunu števila ciljnih organizmov v vzorcu. Zaradi nizke selektivnosti diferencialnega agarskega gojišča je mogoče, da rast v ozadju vpliva na zanesljivo ugotavljanje števila E. coli in koliformnih bakterij, na primer v površinskih vodah ali plitvih vodah v vodnjakih. Ta metoda ni primerna za te vrste vod.
Ta del standarda ISO 9308 je še posebej primeren za vode z nizkim številom bakterij, ki bi povzročile manj kot 100 kolonij skupaj na kromogenem koliformnem agarju (CAA). Te so morda pitna voda, dezinficirana voda v bazenu ali končna voda v čistilnih napravah za pitno vodo.
Nekateri sevi E. coli, ki so negativni na β-D-glukuronidazo, kot so Escherichia coli O157, ne bodo
zaznani kot E. coli. Ker so pozitivni na β-D-glukuronidazo, se bodo na tem kromogenem agarju pojavili kor koliformne bakterije.

General Information

• Status: Published
• Publication Date: 23-Sep-2014
• Withdrawal Date: 30-Mar-2015
• ICS: 07.100.20 - Microbiology of water
• ICS: 13.060.30 - Sewage water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 24-Sep-2014
• Completion Date: 24-Sep-2014
• Ref Project: SIST EN ISO 9308-1:2014 - Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014)

Relations

• Replaces: EN ISO 9308-1:2000 - Water quality - Detection and enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method (ISO 9308-1:2000)
• Effective Date: 01-Oct-2014
• Replaces: EN ISO 9308-1:2000/AC:2008 - Water quality - Detection and enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method (ISO 9308-1:2000/Cor 1:2007)
• Effective Date: 01-Oct-2014
• Amended By: EN ISO 9308-1:2014/A1:2017 - Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016)
• Effective Date: 11-Nov-2015

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 9308-1:2014
01-december-2014
1DGRPHãþD
SIST EN ISO 9308-1:2001
SIST EN ISO 9308-1:2001/AC:2009
.DNRYRVWYRGH8JRWDYOMDQMHãWHYLOD(VFKHULFKLDFROLLQNROLIRUPQLKEDNWHULM
GHO0HWRGDPHPEUDQVNHILOWUDFLMH]DYRGH]PDMKQLPãWHYLORPVSUHPOMDMRþLK
EDNWHULM,62
Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane
filtration method for waters with low bacterial background flora (ISO 9308-1:2014)
Wasserbeschaffenheit - Zählung von Escherichia coli und coliformen - Teil 1:
Membranfiltrationsverfahren für Wässer mit niedriger Begleitflora (ISO 9308-1:2014)
Qualité de l'eau - Dénombrement des Escherichia coli et des bactéries coliformes -
Partie 1: Méthode par filtration sur membrane dans des eaux contenant peu de flore
bactérienne de fond (ISO 9308-1:2014)
Ta slovenski standard je istoveten z: EN ISO 9308-1:2014
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN ISO 9308-1:2014 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 9308-1:2014

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SIST EN ISO 9308-1:2014

EUROPEAN STANDARD
EN ISO 9308-1

NORME EUROPÉENNE

EUROPÄISCHE NORM
September 2014
ICS 07.100.20 Supersedes EN ISO 9308-1:2000
English Version
Water quality - Enumeration of Escherichia coli and coliform
bacteria - Part 1: Membrane filtration method for waters with low
bacterial background flora (ISO 9308-1:2014)
Qualité de l'eau - Dénombrement des Escherichia coli et Wasserbeschaffenheit - Zählung von Escherichia coli und
des bactéries coliformes - Partie 1: Méthode par filtration coliformen Bakterien - Teil 1: Membranfiltrationsverfahren
sur membrane pour les eaux à faible teneur en bactéries für Wässer mit niedriger Begleitflora (ISO 9308-1:2014)
(ISO 9308-1:2014)
This European Standard was approved by CEN on 24 August 2014.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 9308-1:2014 E
worldwide for CEN national Members.

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SIST EN ISO 9308-1:2014
EN ISO 9308-1:2014 (E)
Contents Page
Foreword .3
2

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SIST EN ISO 9308-1:2014
EN ISO 9308-1:2014 (E)
Foreword
This document (EN ISO 9308-1:2014) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is
held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by March 2015, and conflicting national standards shall be withdrawn at
the latest by March 2015.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 9308-1:2000.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hu
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